BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0008.p (217 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLN6.1 B3KLN6_9BACI Spore germination protein 227 7e-58 B1DJT6.1 B1DJT6_9BACL Spore germination protein 177 5e-43 Q9KG71.1 Q9KG71_BACHD Spore germination protein 99 2e-19 C0ZIL7.1 C0ZIL7_BREBN Spore germination protein 97 1e-18 B7HJJ5.1 B7HJJ5_BACC4 Spore germination protein GerD 96 2e-18 >B3KLN6.1 B3KLN6_9BACI Spore germination protein Length = 235 Score = 227 bits (578), Expect = 7e-58, Method: Compositional matrix adjust. Identities = 117/186 (62%), Positives = 144/186 (77%), Gaps = 3/186 (1%) Query: 3 MALTACXXXXXXXXXXXXXYKEMKTMVVDILKSEEGKKAVEEAMXXXXXXXXXXXXXXXX 62 M LTAC YKE+K+MVVDILK++EGKKAVEEA+ Sbjct: 17 MVLTACGVEQSSSPQLG--YKEIKSMVVDILKTDEGKKAVEEALGGGSGGSSGSGGSMGM 74 Query: 63 MKMMPLQTTEQIRVAVKDTITAPEYQKEIEKIMTDPKFAGDFAKAVNSQSKELHLQLIKD 122 M Q+ +QIR+AVKDT+ + EY+KEIEKIMTDP+FAGDFAKA+NS+SK+LH+QLIKD Sbjct: 75 RMMSA-QSGDQIRMAVKDTLVSDEYKKEIEKIMTDPQFAGDFAKAINSESKQLHMQLIKD 133 Query: 123 PTYQKSVAEIMKSPEMMKMFLDLTKTSDYRKQSMTIMQEAMQNPLFRMEVLDLLKTVVQD 182 PTYQK++ +++KSP++MK FL+LT T DYRKQSMT+MQEAMQNPLFRMEVL+LLKTVV+D Sbjct: 134 PTYQKAIQDMLKSPDVMKSFLELTNTPDYRKQSMTVMQEAMQNPLFRMEVLELLKTVVKD 193 Query: 183 ELQPKV 188 ELQPKV Sbjct: 194 ELQPKV 199 >B1DJT6.1 B1DJT6_9BACL Spore germination protein Length = 235 Score = 177 bits (450), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 86/183 (46%), Positives = 128/183 (69%), Gaps = 6/183 (3%) Query: 5 LTACXXXXXXXXXXXXXYKEMKTMVVDILKSEEGKKAVEEAMXXXXXXXXXXXXXXXXMK 64 LT+C YK+MK+MV+DILK+E+ +KA++E+ K Sbjct: 16 LTSCGAESSGGNNQQISYKDMKSMVIDILKTEDAQKALQES------AQQMSGYNGSGTK 69 Query: 65 MMPLQTTEQIRVAVKDTITAPEYQKEIEKIMTDPKFAGDFAKAVNSQSKELHLQLIKDPT 124 ++ +Q EQ+R+AVKD + +PEY K I +MTD +FAG+FAKAVN Q+K++H +L+KDPT Sbjct: 70 LLSVQDQEQVRLAVKDVMVSPEYDKVIRTLMTDTRFAGEFAKAVNKQNKDIHKELMKDPT 129 Query: 125 YQKSVAEIMKSPEMMKMFLDLTKTSDYRKQSMTIMQEAMQNPLFRMEVLDLLKTVVQDEL 184 YQ+ + + K+PEM KM L++ +++ Y+KQ M++MQE+MQNPLFR+EVLDL+K VQ+EL Sbjct: 130 YQEQMIKAFKTPEMDKMILEVLQSTQYKKQVMSLMQESMQNPLFRLEVLDLMKKAVQEEL 189 Query: 185 QPK 187 +PK Sbjct: 190 KPK 192 >Q9KG71.1 Q9KG71_BACHD Spore germination protein Length = 212 Score = 99.4 bits (246), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 53/147 (36%), Positives = 95/147 (64%), Gaps = 12/147 (8%) Query: 22 YKEMKTMVVDILKSEEGKKAVEEAMXXXXXXXXXXXXXXXXMKMMPLQTTEQIRVAVKDT 81 Y+ K M+VD+L++EEGK+A++E M ++ + ++ A++DT Sbjct: 32 YESTKKMMVDMLQTEEGKQAIKELMADEE------------VRRTVVMDNAFVKKAIQDT 79 Query: 82 ITAPEYQKEIEKIMTDPKFAGDFAKAVNSQSKELHLQLIKDPTYQKSVAEIMKSPEMMKM 141 +T+ ++ ++ M DP+FA FA+++ +++++L L+KDP YQ+ + E++++PEM +M Sbjct: 80 LTSEAGKQFWQETMQDPEFAKTFAESMQAENEKLLKGLMKDPEYQQMMVEVLQNPEMEQM 139 Query: 142 FLDLTKTSDYRKQSMTIMQEAMQNPLF 168 FLDL KT +YR+Q MTIM EA ++P F Sbjct: 140 FLDLMKTKEYRQQVMTIMNEAFESPYF 166 >C0ZIL7.1 C0ZIL7_BREBN Spore germination protein Length = 218 Score = 97.1 bits (240), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 58/181 (32%), Positives = 100/181 (55%), Gaps = 13/181 (7%) Query: 2 VMALTACXXXXXXXXXXXXXYKEMKTMVVDILKSEEGKKAVEEAMXXXXXXXXXXXXXXX 61 + LT+C YK +K MV+DIL+++E K++V + M Sbjct: 15 CLVLTSCGGGGQAQSSSQPDYKSVKDMVLDILQTDEAKQSVGKMMKDDKFKQ-------- 66 Query: 62 XMKMMPLQTTEQIRVAVKDTITAPEYQKEIEKIMTDPKFAGDFAKAVNSQSKELHLQLIK 121 +M ++T +R + ++T P I++ DPKFA AK++ ++ K L L+K Sbjct: 67 --NLMLDEST--VRTTLIQSMTNPN-NTHIKEAFKDPKFASAMAKSMKNEQKTLMKDLMK 121 Query: 122 DPTYQKSVAEIMKSPEMMKMFLDLTKTSDYRKQSMTIMQEAMQNPLFRMEVLDLLKTVVQ 181 DP YQK + +MK PE K +DL K+S YR+Q+M +M++++Q+PLF+ E++ ++ V + Sbjct: 122 DPEYQKELISVMKDPEFEKNLMDLMKSSAYRQQTMQVMKDSLQSPLFQAELMKIMTKVSE 181 Query: 182 D 182 D Sbjct: 182 D 182 >B7HJJ5.1 B7HJJ5_BACC4 Spore germination protein GerD Length = 205 Score = 96.3 bits (238), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 56/181 (30%), Positives = 99/181 (54%), Gaps = 14/181 (7%) Query: 1 MVMALTACXXXXXXXXXXXXXYKEMKTMVVDILKSEEGKKAVEEAMXXXXXXXXXXXXXX 60 +++AL AC Y + K M+VDILK+++GKKA+++ + Sbjct: 13 LLIALAACAQGKETKSELD--YDQTKKMIVDILKTDQGKKAIQDVLTDEK---------- 60 Query: 61 XXMKMMPLQTTEQIRVAVKDTITAPEYQKEIEKIMTDPKFAGDFAKAVNSQSKELHLQLI 120 MK + ++ ++D + + + Q+ EK+ DPKF+ FAK++ + +L L+ Sbjct: 61 --MKQALILDETVVKKTIEDAMVSDKGQQFWEKLFKDPKFSSKFAKSMGKEQTQLMKTLL 118 Query: 121 KDPTYQKSVAEIMKSPEMMKMFLDLTKTSDYRKQSMTIMQEAMQNPLFRMEVLDLLKTVV 180 KDP YQ V EIMK+PE+ KM L K+ +YR+ ++ E ++PLF+ +++D++ V Sbjct: 119 KDPEYQAGVIEIMKNPEVEKMMLQTMKSKEYRQYLQQVLTETAESPLFQAKMIDIISKGV 178 Query: 181 Q 181 Q Sbjct: 179 Q 179 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.127 0.334 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 727,572,460 Number of extensions: 21809767 Number of successful extensions: 73992 Number of sequences better than 1.0e-05: 99 Number of HSP's gapped: 73690 Number of HSP's successfully gapped: 99 Length of query: 217 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 87 Effective length of database: 1,835,964,253 Effective search space: 159728890011 Effective search space used: 159728890011 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)