BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0022.p (293 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B4AI51.1 B4AI51_BACPU 3-hydroxybutyryl-coa dehydrogenase (Beta-h... 351 4e-95 A8FAQ7.1 A8FAQ7_BACP2 Possible 3-hydroxybutyryl-CoA dehydrogenas... 348 3e-94 Q7BQ74.1 Q7BQ74_BACCE ZmaG 292 2e-77 C3FUA6.1 C3FUA6_BACTB 3-hydroxybutyryl-CoA dehydrogenase 292 2e-77 C3DAQ5.1 C3DAQ5_BACTU 3-hydroxybutyryl-CoA dehydrogenase 292 2e-77 >B4AI51.1 B4AI51_BACPU 3-hydroxybutyryl-coa dehydrogenase (Beta-hydroxybutyryl-coa dehydrogenase) (Bhbd) EC=1.1.1.157 Length = 285 Score = 351 bits (901), Expect = 4e-95, Method: Compositional matrix adjust. Identities = 167/278 (60%), Positives = 211/278 (75%) Query: 7 IGIIGAGVMGSDLALDLASQGYRVILKDIHEEALEKAKVKIREDFKLVKLMKKELPVKKI 66 +G+IGAGVMG+D+ALDL++ GY+V L D+ EE L +A KI++ ++LV+ ++K+ + Sbjct: 6 VGVIGAGVMGADMALDLSANGYQVTLVDVTEEKLNEALEKIKKTYQLVQFVRKKKISLSL 65 Query: 67 EQVLSNIMFTTTYQSFHAVPFVIENITENWEAKQKVYLELQEHCQEDTIYGVNTSCISIT 126 E VLS I +T+ + H VIEN+TE+WE K+ +Y EL++ C ++TIY VNTSCISIT Sbjct: 66 EDVLSQIQLSTSLEGLHDAHIVIENVTEDWEIKKPIYEELRDICAKETIYFVNTSCISIT 125 Query: 127 KVGAILPRPEHAIGMHFMNPVPLKQMVEIIKGYYTSEDTIRKIKDFLKAIDKKPVLVNDS 186 KVG+++ PE+ IG HFMNPVPLK +VE+I+G TS+DT+ KDFLK+ DK PV+V+D Sbjct: 126 KVGSLMHHPENVIGAHFMNPVPLKALVEVIRGKATSDDTVETAKDFLKSFDKTPVVVHDF 185 Query: 187 PGFVTNRVLMPMINECIWAVHDGVAAPQDVDKIFRLGFSHKMGPLATADLIGLDTILNSI 246 PGFV+NRVLM INE IWAV D VA P DVDKIFR GF HKMGPLAT DLIGLDTILNS+ Sbjct: 186 PGFVSNRVLMLTINEAIWAVQDQVATPADVDKIFRGGFGHKMGPLATGDLIGLDTILNSL 245 Query: 247 LVLYESYNDPKYRPCPLLVKMVDAXXXXXXXXXXFFDY 284 LVLYESY DPK+RPCPLLVKMVDA FF Y Sbjct: 246 LVLYESYKDPKFRPCPLLVKMVDAGEYGKKSGKGFFQY 283 >A8FAQ7.1 A8FAQ7_BACP2 Possible 3-hydroxybutyryl-CoA dehydrogenase EC=1.1.1.157 Length = 285 Score = 348 bits (893), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 165/278 (59%), Positives = 210/278 (75%) Query: 7 IGIIGAGVMGSDLALDLASQGYRVILKDIHEEALEKAKVKIREDFKLVKLMKKELPVKKI 66 +G+IGAGVMG+D+ALDL++ GY+V L D+ EE L +A KI++ ++LV+ ++K+ + Sbjct: 6 VGVIGAGVMGADMALDLSANGYQVTLVDVTEEKLNEALEKIKKTYQLVQFVRKKKISLSL 65 Query: 67 EQVLSNIMFTTTYQSFHAVPFVIENITENWEAKQKVYLELQEHCQEDTIYGVNTSCISIT 126 E VLS I +T+ + H +IEN+TE+WE K+ +Y EL++ C ++TIY VNTSCISIT Sbjct: 66 EDVLSQIQLSTSLEGLHDAHIIIENVTEDWEIKKPIYEELRDICAKETIYFVNTSCISIT 125 Query: 127 KVGAILPRPEHAIGMHFMNPVPLKQMVEIIKGYYTSEDTIRKIKDFLKAIDKKPVLVNDS 186 KVG+++ PE+ IG HFMNPVPLK +VE+I+G TS+DT+ K FLK+ DK PV+V+D Sbjct: 126 KVGSLMHHPENVIGAHFMNPVPLKALVEVIRGKATSDDTVETAKGFLKSFDKTPVVVHDF 185 Query: 187 PGFVTNRVLMPMINECIWAVHDGVAAPQDVDKIFRLGFSHKMGPLATADLIGLDTILNSI 246 PGFV+NRVLM INE IWAV D VA P DVDKIFR GF HKMGPLAT DLIGLDTILNS+ Sbjct: 186 PGFVSNRVLMLTINEAIWAVQDQVATPADVDKIFRGGFGHKMGPLATGDLIGLDTILNSL 245 Query: 247 LVLYESYNDPKYRPCPLLVKMVDAXXXXXXXXXXFFDY 284 LVLYESY DPK+RPCPLLVKMVDA FF Y Sbjct: 246 LVLYESYKDPKFRPCPLLVKMVDAGEYGKKSGKGFFQY 283 >Q7BQ74.1 Q7BQ74_BACCE ZmaG Length = 282 Score = 292 bits (748), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 141/281 (50%), Positives = 186/281 (66%) Query: 4 FETIGIIGAGVMGSDLALDLASQGYRVILKDIHEEALEKAKVKIREDFKLVKLMKKELPV 63 F+ IGI+GAG MG +A+DL G +L D+ EE LEKA+ KI E + L+ K P Sbjct: 2 FKKIGIVGAGTMGIGMAVDLVLHGLETVLIDVTEEQLEKAEEKILETVRFAPLINKAFPR 61 Query: 64 KKIEQVLSNIMFTTTYQSFHAVPFVIENITENWEAKQKVYLELQEHCQEDTIYGVNTSCI 123 E+VLS I +T +++EN+ ENW+ K+ +Y L E C++DTI+GVNTSCI Sbjct: 62 MHKEEVLSLISSSTNLDEVAECDYIVENVPENWQIKEPIYRRLDEICKKDTIFGVNTSCI 121 Query: 124 SITKVGAILPRPEHAIGMHFMNPVPLKQMVEIIKGYYTSEDTIRKIKDFLKAIDKKPVLV 183 SITKVG + RP+ IGMHFMNPV +K +E+I+G+ TS++T+ K + FLK +DK ++V Sbjct: 122 SITKVGGVTKRPDKIIGMHFMNPVYMKPSIEVIRGHLTSDETVEKAQSFLKQLDKDAIVV 181 Query: 184 NDSPGFVTNRVLMPMINECIWAVHDGVAAPQDVDKIFRLGFSHKMGPLATADLIGLDTIL 243 ND GFV+NR+ +NE W V DGVA P+ VD IF+ F H MGPL TADLIGLDT+L Sbjct: 182 NDQTGFVSNRISHLFMNESAWVVMDGVATPKQVDDIFKKCFGHTMGPLETADLIGLDTVL 241 Query: 244 NSILVLYESYNDPKYRPCPLLVKMVDAXXXXXXXXXXFFDY 284 +S+ VLYE Y DPK+R CPLL KMVDA F+ Y Sbjct: 242 HSLNVLYEEYQDPKFRCCPLLKKMVDAGECGRKSGKGFYAY 282 >C3FUA6.1 C3FUA6_BACTB 3-hydroxybutyryl-CoA dehydrogenase Length = 282 Score = 292 bits (748), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 141/281 (50%), Positives = 186/281 (66%) Query: 4 FETIGIIGAGVMGSDLALDLASQGYRVILKDIHEEALEKAKVKIREDFKLVKLMKKELPV 63 F+ IGI+GAG MG +A+DL G +L D+ EE LEKA+ KI E + L+ K P Sbjct: 2 FKKIGIVGAGTMGIGMAVDLVLHGLETVLIDVTEEQLEKAEEKILETVRFAPLINKAFPR 61 Query: 64 KKIEQVLSNIMFTTTYQSFHAVPFVIENITENWEAKQKVYLELQEHCQEDTIYGVNTSCI 123 E+VLS I +T +++EN+ ENW+ K+ +Y L E C++DTI+GVNTSCI Sbjct: 62 MHKEEVLSLISSSTNLDEVAECDYIVENVPENWQIKEPIYRRLDEICKKDTIFGVNTSCI 121 Query: 124 SITKVGAILPRPEHAIGMHFMNPVPLKQMVEIIKGYYTSEDTIRKIKDFLKAIDKKPVLV 183 SITKVG + RP+ IGMHFMNPV +K +E+I+G+ TS++T+ K + FLK +DK ++V Sbjct: 122 SITKVGGVTKRPDKIIGMHFMNPVYMKPSIEVIRGHLTSDETVEKAQSFLKQLDKDAIVV 181 Query: 184 NDSPGFVTNRVLMPMINECIWAVHDGVAAPQDVDKIFRLGFSHKMGPLATADLIGLDTIL 243 ND GFV+NR+ +NE W V DGVA P+ VD IF+ F H MGPL TADLIGLDT+L Sbjct: 182 NDQTGFVSNRISHLFMNESAWVVMDGVATPKQVDDIFKKCFGHTMGPLETADLIGLDTVL 241 Query: 244 NSILVLYESYNDPKYRPCPLLVKMVDAXXXXXXXXXXFFDY 284 +S+ VLYE Y DPK+R CPLL KMVDA F+ Y Sbjct: 242 HSLNVLYEEYQDPKFRCCPLLKKMVDAGECGRKSGKGFYAY 282 >C3DAQ5.1 C3DAQ5_BACTU 3-hydroxybutyryl-CoA dehydrogenase Length = 282 Score = 292 bits (748), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 141/281 (50%), Positives = 186/281 (66%) Query: 4 FETIGIIGAGVMGSDLALDLASQGYRVILKDIHEEALEKAKVKIREDFKLVKLMKKELPV 63 F+ IGI+GAG MG +A+DL G +L D+ EE LEKA+ KI E + L+ K P Sbjct: 2 FKKIGIVGAGTMGIGMAVDLVLHGLETVLIDVTEEQLEKAEEKILETVRFAPLINKAFPR 61 Query: 64 KKIEQVLSNIMFTTTYQSFHAVPFVIENITENWEAKQKVYLELQEHCQEDTIYGVNTSCI 123 E+VLS I +T +++EN+ ENW+ K+ +Y L E C++DTI+GVNTSCI Sbjct: 62 MHKEEVLSLISSSTNLDEVAECDYIVENVPENWQIKEPIYRRLDEICKKDTIFGVNTSCI 121 Query: 124 SITKVGAILPRPEHAIGMHFMNPVPLKQMVEIIKGYYTSEDTIRKIKDFLKAIDKKPVLV 183 SITKVG + RP+ IGMHFMNPV +K +E+I+G+ TS++T+ K + FLK +DK ++V Sbjct: 122 SITKVGGVTKRPDKIIGMHFMNPVYMKPSIEVIRGHLTSDETVEKAQSFLKQLDKDAIVV 181 Query: 184 NDSPGFVTNRVLMPMINECIWAVHDGVAAPQDVDKIFRLGFSHKMGPLATADLIGLDTIL 243 ND GFV+NR+ +NE W V DGVA P+ VD IF+ F H MGPL TADLIGLDT+L Sbjct: 182 NDQTGFVSNRISHLFMNESAWVVMDGVATPKQVDDIFKKCFGHTMGPLETADLIGLDTVL 241 Query: 244 NSILVLYESYNDPKYRPCPLLVKMVDAXXXXXXXXXXFFDY 284 +S+ VLYE Y DPK+R CPLL KMVDA F+ Y Sbjct: 242 HSLNVLYEEYQDPKFRCCPLLKKMVDAGECGRKSGKGFYAY 282 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,649,926,965 Number of extensions: 65742696 Number of successful extensions: 219666 Number of sequences better than 1.0e-05: 3301 Number of HSP's gapped: 214293 Number of HSP's successfully gapped: 3464 Length of query: 293 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 159 Effective length of database: 1,798,280,193 Effective search space: 285926550687 Effective search space used: 285926550687 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)