BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0019.p (330 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9KNN1.1 A9KNN1_CLOPH Lipolytic protein G-D-S-L family 339 3e-91 B0MN26.1 B0MN26_9FIRM Putative uncharacterized protein 311 7e-83 O13496.1 O13496_NEOPA Acetylxylan esterase (Fragment) 258 4e-67 B4BD29.1 B4BD29_CLOTM Glycoside hydrolase family 5 147 1e-33 P10477.1 GUNE_CLOTM Endoglucanase E EC=3.2.1.4 147 2e-33 >A9KNN1.1 A9KNN1_CLOPH Lipolytic protein G-D-S-L family Length = 357 Score = 339 bits (869), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 173/333 (51%), Positives = 223/333 (66%), Gaps = 4/333 (1%) Query: 1 VLWLALSGGGIEFSFCGTK-AEITIKGDAI--ASTGNNLARIGISVNGKRVIDDQVDQPL 57 + WLALSG GIEF C T+ EI I D+ G + AR + +N + V D + + Sbjct: 26 IRWLALSGSGIEF-ICETELVEIAIVSDSSVHGQNGTDYARFAMYINEELVFCDTLREEQ 84 Query: 58 KSYTVFESETAQNVVIRVIKLSEAAMSTVGVQCISVHAADGIKPTPDKIHTIEFIGDSIT 117 K+ +FES Q + +R++K+SEA S +G++ I +H+ K T D H IEFIGDSIT Sbjct: 85 KTVKIFESIEKQEITVRILKISEAPHSMIGIEKIILHSDIPPKKTEDLAHKIEFIGDSIT 144 Query: 118 CGYGVDDEHELHSFSTATEDVTKTYAYLTAEQLEADYSMVSYSGYGIITGYTENDHKLIT 177 CGYGVD E EL F+TATEDVTK YAYLT++ L +DYSMVSYSGYGI++GYTEND K Sbjct: 145 CGYGVDAESELIHFTTATEDVTKAYAYLTSKALNSDYSMVSYSGYGIVSGYTENDKKDTI 204 Query: 178 HLLPDYYEKVGKSEGKFDNRLLPQDIRWDFRKFVPDLIVINLGTNDDSYTKEDTAKQTEY 237 ++P YY+ V + G F + L ++I WDF K++P+LIVINLGTND++Y K+ T + EY Sbjct: 205 QIVPKYYQSVARCFGTFKDLPLLENIPWDFSKYIPELIVINLGTNDNTYCKDKTKRHEEY 264 Query: 238 AEEYVKFLKKVRRNNPHAPILCTLGIMGDRLYPYVEQAVKQFIRETGDSKITAMKLDEQL 297 +EYVKFLK VR N A ILC LG+MG LYP VE+AV + ET D I +L EQ Sbjct: 265 RDEYVKFLKVVREKNMDATILCALGLMGANLYPMVEEAVALYQSETKDDNIVTFQLTEQD 324 Query: 298 TADGYAADFHPSRATHSKAAKQLTSYIKELMKW 330 ADGY AD+HPS+ H KAAKQLT IK++M W Sbjct: 325 KADGYGADWHPSKVAHQKAAKQLTEEIKKIMGW 357 >B0MN26.1 B0MN26_9FIRM Putative uncharacterized protein Length = 358 Score = 311 bits (796), Expect = 7e-83, Method: Compositional matrix adjust. Identities = 161/332 (48%), Positives = 220/332 (66%), Gaps = 4/332 (1%) Query: 2 LWLALSGGGIEFSFCGTKAEITIKGDAIASTGN--NLARIGISVNGKRVIDDQVDQPLKS 59 LW A SG G EF F G K EIT+ GD ++ GN N ARI I V+ +RV+DD +D+ K+ Sbjct: 27 LWAAFSGAGAEFIFTGKKLEITVIGDFASTAGNDENYARIAIYVDNERVVDDMLDEKEKT 86 Query: 60 YTVFESETAQNVVIRVIKLSEAAMSTVGVQCISVHAADGIKPTPDKIHTIEFIGDSITCG 119 Y V ESE+A+ +R+IKLSE AMST G++ + + + I+P P K +EFIGDSITCG Sbjct: 87 YKVLESESAECRNVRIIKLSECAMSTFGIKPVMIAEDEKIEPAPAKNIKMEFIGDSITCG 146 Query: 120 YGVDDEHELHSFSTATEDVTKTYAYLTAEQLEADYSMVSYSGYGIITGYTENDHKLITHL 179 YGVDD + H F TATEDVTK YAY TA L ADYSMVS SGYGII+G+T + +K+ Sbjct: 147 YGVDDPDKEHHFKTATEDVTKAYAYKTALALNADYSMVSVSGYGIISGFTNDGNKIPQQT 206 Query: 180 LPDYYEKVGKSEGKFDNRLLPQDIRWDFRKFVPDLIVINLGTNDDSYTKEDTAKQTEYAE 239 +P YY+K+G S KF + + + WDF ++ PD+IVINLGTND +Y D ++ E+ + Sbjct: 207 IPQYYDKLGFSYNKFADSITVSETEWDFERYKPDIIVINLGTNDMNYATTD-ERKAEFED 265 Query: 240 EYVKFLKKVRRNNPHAPILCTLGIMGDRLYPYVEQAVKQFIRETGDSKITAMKLDEQ-LT 298 Y+ FLKKVR NP + I T G+MG L +E ++++ ETG+ +IT + L Q Sbjct: 266 GYLDFLKKVRSLNPDSYIFQTYGVMGTSLEENIENVRRKYMSETGNERITFIPLTMQDED 325 Query: 299 ADGYAADFHPSRATHSKAAKQLTSYIKELMKW 330 ADG AD+HPS THS ++++T+ IKE + + Sbjct: 326 ADGIVADWHPSPKTHSLVSQKVTAAIKETLGY 357 >O13496.1 O13496_NEOPA Acetylxylan esterase (Fragment) Length = 392 Score = 258 bits (660), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 143/336 (42%), Positives = 212/336 (63%), Gaps = 11/336 (3%) Query: 2 LWLALSGGGIEFSFCGTKAEITIKGDAIASTGNNLARIGISVNGKRVIDDQVDQPLKSYT 61 LW+ L+ G+E+ F G I++ D+ A++ +N ARI I +G+ D V + Sbjct: 61 LWIGLTDSGVEYKFNGKTTTISLTADSSAASTDNPARIVILADGELYEDTTVKSVESEFE 120 Query: 62 VFESETAQNVVIRVIKLSEAAMSTVGVQCISVHAADGIKPTPDKIHTIEFIGDSITCGYG 121 V + ++ V R +K+SE A TV + I A + I+PT D IEFIGDSITC YG Sbjct: 121 VTFDASGEHTV-RFMKVSECANGTVRITAIKADA-EKIEPTADADKKIEFIGDSITCAYG 178 Query: 122 VDDEHELHSFSTATEDVTKTYAYLTAEQLEADYSMVSYSGYGIITGYTENDHKLITHLLP 181 VD +FST TED TK+YAYL ++QL ADYSM S+SG+GII+GY+ + + +P Sbjct: 179 VDGTE--GTFSTKTEDGTKSYAYLASKQLNADYSMFSFSGFGIISGYSTDGTRNEVSTVP 236 Query: 182 DYYEKVGKS-EGKFDNRLLP-QDIRWDFRKFVPDLIVINLGTNDDSY---TKEDTAK-QT 235 YYEK+G S +F + + +++ WD +FVPDL+VINLGTND+SY K D AK + Sbjct: 237 QYYEKLGFSYWTQFGSDITQLKEVSWDASQFVPDLVVINLGTNDNSYMQNVKGDRAKEEA 296 Query: 236 EYAEEYVKFLKKVRRNNPHAPILCTLGIMGDRLYPYVEQAVKQFIRETGDSKITAMKLDE 295 ++ +YVKF++++R +P+A ILCTLGIMG LYP +E+AV + +GD K+ A K ++ Sbjct: 297 DFVADYVKFIEQIRSVHPNAEILCTLGIMGQELYPQIEEAVSTYKAASGDEKVNAYKFNQ 356 Query: 296 Q-LTADGYAADFHPSRATHSKAAKQLTSYIKELMKW 330 Q + +G D+HP+ +H +AA++L + IK+L W Sbjct: 357 QNINKNGKGIDWHPAPQSHVEAAEELVAEIKKLYGW 392 >B4BD29.1 B4BD29_CLOTM Glycoside hydrolase family 5 Length = 827 Score = 147 bits (371), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 111/344 (32%), Positives = 163/344 (47%), Gaps = 56/344 (16%) Query: 5 ALSGGGIEFSFCGTKAEITIKGDAIASTGNNLARIGISVNGKRVIDDQVDQPLKSYTVFE 64 A SG +E +F GT+A +TIK S G N + + N PL ++V Sbjct: 522 AWSGSNVELNFYGTEASVTIK-----SGGENWFQAIVDGN-----------PLPPFSVNA 565 Query: 65 SETAQNVV---------IRVIKLSEAAMSTVGVQCISVHAADG---IKPTPDKIHTIEFI 112 + + +V + + K +EA++ V Q + G P P + IEFI Sbjct: 566 TTSTVKLVSGLAEGAHHLVLWKRTEASLGEV--QFLGFDFGSGKLLAAPKPLE-RKIEFI 622 Query: 113 GDSITCGYGVDDEHELHSFSTATEDVTKTYAYLTAEQLEADYSMVSYSGYGIITGYTEND 172 GDSITC YG + + SF+ E+ +YA +TA L A +M+++SG G+ Y Sbjct: 623 GDSITCAYGNEGTSKEQSFTPKNENSYMSYAAITARNLNASANMIAWSGIGLTMNYGGAP 682 Query: 173 HKLITHLLPDYYEKVGKSEGKFDNRLLP-QDIRWDFRKFVPDLIVINLGTNDDSYTKEDT 231 LI P LP +RWDF K+VP ++VINLGTND S + D Sbjct: 683 GPLIMDRYP---------------YTLPYSGVRWDFSKYVPQVVVINLGTNDFSTSFAD- 726 Query: 232 AKQTEYAEEYVKFLKKVRRNNPHAPILCTLGIM-----GDRLYPYVEQAVKQFIRETGDS 286 +T++ Y + +VRRN P A I C +G M D YV + V R +GD Sbjct: 727 --KTKFVTAYKNLISEVRRNYPDAHIFCCVGPMLWGTGLDLCRSYVTEVVNDCNR-SGDL 783 Query: 287 KITAMKLDEQLTADGYAADFHPSRATHSKAAKQLTSYIKELMKW 330 K+ ++ +Q + GY D+HPS ATH A++LT+ IK + W Sbjct: 784 KVYFVEFPQQDGSTGYGEDWHPSIATHQLMAERLTAEIKNKLGW 827 >P10477.1 GUNE_CLOTM Endoglucanase E EC=3.2.1.4 Length = 814 Score = 147 bits (370), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 111/344 (32%), Positives = 163/344 (47%), Gaps = 56/344 (16%) Query: 5 ALSGGGIEFSFCGTKAEITIKGDAIASTGNNLARIGISVNGKRVIDDQVDQPLKSYTVFE 64 A SG +E +F GT+A +TIK S G N + + N PL ++V Sbjct: 509 AWSGSNVELNFYGTEASVTIK-----SGGENWFQAIVDGN-----------PLPPFSVNA 552 Query: 65 SETAQNVV---------IRVIKLSEAAMSTVGVQCISVHAADG---IKPTPDKIHTIEFI 112 + + +V + + K +EA++ V Q + G P P + IEFI Sbjct: 553 TTSTVKLVSGLAEGAHHLVLWKRTEASLGEV--QFLGFDFGSGKLLAAPKPLE-RKIEFI 609 Query: 113 GDSITCGYGVDDEHELHSFSTATEDVTKTYAYLTAEQLEADYSMVSYSGYGIITGYTEND 172 GDSITC YG + + SF+ E+ +YA +TA L A +M+++SG G+ Y Sbjct: 610 GDSITCAYGNEGTSKEQSFTPKNENSYMSYAAITARNLNASANMIAWSGIGLTMNYGGAP 669 Query: 173 HKLITHLLPDYYEKVGKSEGKFDNRLLP-QDIRWDFRKFVPDLIVINLGTNDDSYTKEDT 231 LI P LP +RWDF K+VP ++VINLGTND S + D Sbjct: 670 GPLIMDRYP---------------YTLPYSGVRWDFSKYVPQVVVINLGTNDFSTSFAD- 713 Query: 232 AKQTEYAEEYVKFLKKVRRNNPHAPILCTLGIM-----GDRLYPYVEQAVKQFIRETGDS 286 +T++ Y + +VRRN P A I C +G M D YV + V R +GD Sbjct: 714 --KTKFVTAYKNLISEVRRNYPDAHIFCCVGPMLWGTGLDLCRSYVTEVVNDCNR-SGDL 770 Query: 287 KITAMKLDEQLTADGYAADFHPSRATHSKAAKQLTSYIKELMKW 330 K+ ++ +Q + GY D+HPS ATH A++LT+ IK + W Sbjct: 771 KVYFVEFPQQDGSTGYGEDWHPSIATHQLMAERLTAEIKNKLGW 814 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,011,029,121 Number of extensions: 83686588 Number of successful extensions: 212927 Number of sequences better than 1.0e-05: 65 Number of HSP's gapped: 212772 Number of HSP's successfully gapped: 65 Length of query: 330 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 194 Effective length of database: 1,779,438,163 Effective search space: 345211003622 Effective search space used: 345211003622 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)