BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0184.0014.p
         (199 letters)

Database: pfamseq 
           9,421,015 sequences; 3,060,696,203 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

C4CX58.1 C4CX58_9SPHI Glycogen debranching enzyme                     120   8e-26
A4CIT1.1 A4CIT1_9FLAO Predicted glycogen debranching enzyme           120   9e-26
A5UU77.1 A5UU77_ROSS1 Amylo-alpha-1,6-glucosidase                      69   3e-10
A7NL81.1 A7NL81_ROSCS Amylo-alpha-16-glucosidase                       69   3e-10

>C4CX58.1 C4CX58_9SPHI Glycogen debranching enzyme
          Length = 815

 Score =  120 bits (301), Expect = 8e-26,   Method: Compositional matrix adjust.
 Identities = 62/190 (32%), Positives = 98/190 (51%), Gaps = 26/190 (13%)

Query: 1   MEAGIAEPDKARRALDNMRNDTFIGEYGTYLSGM---------------------FQQGT 39
           ME G+A+PDKA  AL       F   +G +++G+                     +    
Sbjct: 588 METGVADPDKAAIALRTASK--FTNPFGVFVTGIDRDESAGTDEGSFNLNKKVFTYTGAV 645

Query: 40  MTISTGVHAVAEAASGNPDAALALLGRMLRSFSRVLPGSFSEMSPDYGCVVQAWTIYALA 99
           MT+ TGV  + E   G+PD AL  L RM ++FS   PGS  E+SPDYG V QAW +Y+ A
Sbjct: 646 MTLPTGVATIGENNYGHPDEALGYLKRMTKTFSYAFPGSIYEVSPDYGMVTQAWNMYSYA 705

Query: 100 VPVVRHFFGAEPFAARKELRLKPQLPSAWSGKACTLERLVIGEAEFGLSLTEKDGVLQ-A 158
           VP+++ FFG  P A+R+ + ++PQ+PS W      +E++ +G+    ++  +    L  +
Sbjct: 706 VPIIKQFFGIRPEASRQLVTIRPQMPSDWDN--ARVEKVTVGDNLLSMAYAKAGQQLTLS 763

Query: 159 LIHNEAGWKV 168
           +   +  W V
Sbjct: 764 ITQKQPNWTV 773


>A4CIT1.1 A4CIT1_9FLAO Predicted glycogen debranching enzyme
          Length = 804

 Score =  120 bits (300), Expect = 9e-26,   Method: Compositional matrix adjust.
 Identities = 67/161 (41%), Positives = 86/161 (53%), Gaps = 24/161 (14%)

Query: 1   MEAGIAEPDKARRALDNMRNDTFIGEYGTYLSGM--------------------FQQGTM 40
           ME GIA+  KA  AL+  R   F   +G +++G+                    +    M
Sbjct: 577 MEMGIADSAKAYTALETARK--FTNPFGVFVTGIDRDESAGSDSGSFKGSSAFSYTGAVM 634

Query: 41  TISTGVHAVAEAASGNPDAALALLGRMLRSFSRVLPGSFSEMSPDYGCVVQAWTIYALAV 100
           T+ TGV AVAE   G PD AL  L RM RSFS  LPGS  E+SPDYG + QAW IYA AV
Sbjct: 635 TLPTGVQAVAENNYGRPDQALDYLRRMTRSFSYALPGSMYEVSPDYGMIAQAWNIYAFAV 694

Query: 101 PVVRHFFGAEPFAARKELRLKPQLPSAWSGKACTLERLVIG 141
           P+V  FFG  P A+ + +R+ P +P  W     +LE + IG
Sbjct: 695 PIVEQFFGISPRASDRLIRIDPLMPEDW--DVASLENVSIG 733


>A5UU77.1 A5UU77_ROSS1 Amylo-alpha-1,6-glucosidase
          Length = 676

 Score = 68.6 bits (166), Expect = 3e-10,   Method: Compositional matrix adjust.
 Identities = 44/149 (29%), Positives = 72/149 (48%), Gaps = 3/149 (2%)

Query: 4   GIAEPDKARRALDNMRNDTFIGEYGTYLSGMFQQGTMTISTGVHAVAEAASGNPDAALAL 63
           GIA P++A+R LD +  + ++ E+G   +        T+ TG+ A+A    G+ D  + L
Sbjct: 489 GIAAPERAQRVLDRIVRE-WVNEWGMVHTRGADMRVWTLPTGLLALAAFRHGDADTGIQL 547

Query: 64  LGRMLRSFSRVLPGSFSEMSPDYGCVVQAWTIYALAVPVVRHFFGAEPFAARKELRLKPQ 123
           L  +  +      G+  E+ P   C +Q W+       +    FG  P A R EL ++P+
Sbjct: 548 LRNIGVTARYGTLGTLKELIPQGLCFIQLWSAALFVQGITEGLFGLSPHAHRHELIIEPR 607

Query: 124 LPSAWSGKACTLERLVIGEAEFGLSLTEK 152
           LPS WS    TL  L +G+    L +T +
Sbjct: 608 LPSGWS--EATLHGLRVGDQTLDLHVTPE 634


>A7NL81.1 A7NL81_ROSCS Amylo-alpha-16-glucosidase
          Length = 676

 Score = 68.6 bits (166), Expect = 3e-10,   Method: Compositional matrix adjust.
 Identities = 47/159 (29%), Positives = 75/159 (47%), Gaps = 3/159 (1%)

Query: 4   GIAEPDKARRALDNMRNDTFIGEYGTYLSGMFQQGTMTISTGVHAVAEAASGNPDAALAL 63
           GIA P++A+R LD +  + ++ E+G   +        T+ TG+ A+A   +G  D  L L
Sbjct: 489 GIATPERAQRTLDRIMRE-WVSEWGMLHTRGADSRVWTLPTGLLALAAFRTGRADDGLRL 547

Query: 64  LGRMLRSFSRVLPGSFSEMSPDYGCVVQAWTIYALAVPVVRHFFGAEPFAARKELRLKPQ 123
           L  +  +      G+  E+ P   C +Q W+   L   V    FG  P A R EL ++P 
Sbjct: 548 LRCIGETARHGTLGTLKELIPQGLCFIQLWSAGLLVQGVTEGIFGLHPQAHRHELMVEPH 607

Query: 124 LPSAWSGKACTLERLVIGEAEFGLSLTEKDGVLQALIHN 162
           LP+ W  +  TL  L +GE    L +  +   ++ L  N
Sbjct: 608 LPATW--RHTTLHGLRVGEHTLDLHIAREAITIRHLKGN 644


  Database: pfamseq
    Posted date:  Aug 5, 2010  3:22 PM
  Number of letters in database: 3,060,696,203
  Number of sequences in database:  9,421,015
  
Lambda     K      H
   0.318    0.133    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 9421015
Number of Hits to DB: 1,211,752,628
Number of extensions: 48016554
Number of successful extensions: 108139
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 108136
Number of HSP's successfully gapped: 4
Length of query: 199
Length of database: 3,060,696,203
Length adjustment: 129
Effective length of query: 70
Effective length of database: 1,845,385,268
Effective search space: 129176968760
Effective search space used: 129176968760
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 127 (53.5 bits)