BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0013.p (554 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9U7Q1.1 A9U7Q1_PHYPA Predicted protein (Fragment) 435 e-120 A4CIT1.1 A4CIT1_9FLAO Predicted glycogen debranching enzyme 308 8e-82 C4CX58.1 C4CX58_9SPHI Glycogen debranching enzyme 308 8e-82 Q1AZ91.1 Q1AZ91_RUBXD Glycogen debranching enzyme-like protein 151 2e-34 A5UU77.1 A5UU77_ROSS1 Amylo-alpha-1,6-glucosidase 104 3e-20 >A9U7Q1.1 A9U7Q1_PHYPA Predicted protein (Fragment) Length = 409 Score = 435 bits (1118), Expect = e-120, Method: Compositional matrix adjust. Identities = 211/374 (56%), Positives = 262/374 (70%), Gaps = 1/374 (0%) Query: 145 VGVHDGVRDEPTVLNPREVLVQDEGNPWFTLVGTDLAGEELEVRSDLYGPEFTAGAGCGI 204 +G+HDG D ++ R + +D + WF L+GTDL GE+ + LYGPEFT+G G G+ Sbjct: 1 MGIHDGQEDVIEKVSNRLAIAKDCEHDWFALIGTDLPGEQFD-SGQLYGPEFTSGNGRGV 59 Query: 205 AFRTELTMAAGERRRFHLFVAGSCTSREECEDSYRXXXXXXXXXXXXKRGVYSAVAERAK 264 F T +T+ E+ FHL +AGS SREECE +Y K A+ ERA+ Sbjct: 60 KFETAVTLQPQEQLTFHLLIAGSYASREECERTYSVISGNFDELLEAKMRACEAIRERAE 119 Query: 265 LTVEGEPLWNEAFKWAKWNNQWLIQQVDGTGRGLTAGGPTYPWWFGCDSTYALQGVLAIG 324 L VEGEP N+ F W KWNNQWL+QQVDG GRGLTAG PTYPWWFGCD++Y++QGVLAIG Sbjct: 120 LEVEGEPDINDIFAWVKWNNQWLVQQVDGIGRGLTAGSPTYPWWFGCDNSYSIQGVLAIG 179 Query: 325 DPQLAKDTLNLLLEKSEEVNGNGRFIHELTTLGAVSNRGNTQETAHYIAFVWELFRWTGD 384 D QLAKDT L+L+ S + NGNGR +HE+TT+GAVSN GNTQETAHYIA VWEL+RWTGD Sbjct: 180 DHQLAKDTARLILDSSVKANGNGRIVHEVTTMGAVSNPGNTQETAHYIALVWELYRWTGD 239 Query: 385 EELLRRHYGYCVKGIGWLLGEMDPDGDLFPSGYGIIEIAGLNMELIDSAVYTAKALQAMA 444 +L +++ YCVKGI WLLGEMDPDGDL PSGYGIIEIAGLNMELIDSAVYTAKA+QA A Sbjct: 240 RNILEQNFEYCVKGIDWLLGEMDPDGDLLPSGYGIIEIAGLNMELIDSAVYTAKAVQAAA 299 Query: 445 EMSGYLQEAEAQQKYSELAQRCTAAVNRSFWQENEGLFXXXXXXXXXXXXXXXFLVSLAE 504 MS L + ++Y++L++R AVN +W E EGL+ F+VSLAE Sbjct: 300 FMSRELGDEAKAEQYTQLSERIVRAVNDRYWNEREGLYSDAVAPKKDILPKVEFMVSLAE 359 Query: 505 AQGVTGYREYMDRL 518 +G YR Y++R+ Sbjct: 360 KRGAADYRAYLERM 373 >A4CIT1.1 A4CIT1_9FLAO Predicted glycogen debranching enzyme Length = 804 Score = 308 bits (789), Expect = 8e-82, Method: Compositional matrix adjust. Identities = 168/471 (35%), Positives = 245/471 (52%), Gaps = 9/471 (1%) Query: 13 YYNGGEYVKICGTQDGYFPDFGHHTANEMGGIWLHPIKLLDGFWLKLTDTKRNISVWSRA 72 Y G+ + G QDG FP+ G H EMGGIW HPIKL+DGF + + N + + Sbjct: 52 YVTPGDRAYMVGHQDGSFPEIGWHIPGEMGGIWAHPIKLMDGFQTSI-EVGGNPLPPADS 110 Query: 73 DGFTSEPWGSRFQYDHGLSHIPVAMERTQFAPERVKGMTAAYELHHYGDEDLHLQVELLV 132 F + P + + P+ M R QF P+ + G+ YE + G+E L ++ L Sbjct: 111 TRFVNYPMANSHHW--SWDGQPLRMSRWQFVPDHLPGVALEYEFENTGEEALTVEFSFLG 168 Query: 133 RTDLRPVWFSDEVGVHDGVRDEPTVLNPREVLVQDEGNPWFTLVGTDLAGEE-LEVRSDL 191 +TDLRP W + G+ DG +D +PW+ G + A + L D Sbjct: 169 QTDLRPTWLGERTGMADGRDSAYYDSGINAWTARDGQSPWYVAFGPNRAPDGILPANRD- 227 Query: 192 YGPEFTAGAGCGIAFRTELTMAAGERRRFHLFVAGSCTSREECEDSYRXXXXXXXXXXXX 251 G G +R L + GE R + V GS ++ + Sbjct: 228 ---SIQRGEGIAAGYRFSLKIRPGETERIRMAVGGSTSTEAAARQVQQSILEEASSLLES 284 Query: 252 KRGVYSAVAERAKLTVEGEPLWNEAFKWAKWNNQWLIQQVDGTGRGLTAGGPTYPWWFGC 311 KR Y+ +A R+KLTV L +AF+W K+N+ W+IQ+V G G G+TAG P YPWWFG Sbjct: 285 KRDRYAELAGRSKLTVPDTAL-QQAFEWLKYNSDWMIQEVPGIGTGVTAGIPDYPWWFGV 343 Query: 312 DSTYALQGVLAIGDPQLAKDTLNLLLEKSEEVNGNGRFIHELTTLGAVSNRGNTQETAHY 371 DS YAL+G +A+G ++ T+ L+ S VNGNGR IHE++T GAV N GN ET + Sbjct: 344 DSEYALRGYMAVGQQEVVFQTIRLIDSVSRAVNGNGRIIHEMSTNGAVFNSGNINETPQF 403 Query: 372 IAFVWELFRWTGDEELLRRHYGYCVKGIGWLLGEMDPDGDLFPSGYGIIEIAGLNMELID 431 + +W ++RW+GD + LRR++ G+ WL+ E D DG+ FP G+G++EI GL+ E+ID Sbjct: 404 ASLIWHIYRWSGDRDFLRRYFQTIRDGLDWLMRENDGDGNGFPDGFGMMEIHGLDSEMID 463 Query: 432 SAVYTAKALQAMAEMSGYLQEAEAQQKYSELAQRCTAAVNRSFWQENEGLF 482 A YT + ++ L E + ++Y+ LA A +N FW E G F Sbjct: 464 VASYTQAGFDHASRIAAELGEEDLSRQYAALASELKARINSEFWSERYGSF 514 >C4CX58.1 C4CX58_9SPHI Glycogen debranching enzyme Length = 815 Score = 308 bits (789), Expect = 8e-82, Method: Compositional matrix adjust. Identities = 164/471 (34%), Positives = 246/471 (52%), Gaps = 8/471 (1%) Query: 13 YYNGGEYVKICGTQDGYFPDFGHHTANEMGGIWLHPIKLLDGFWLKLTDTKRNISVWSRA 72 + G + + G QDG FPD G H EMGGIW HPIKL+DGF ++ ++ I + +A Sbjct: 62 FVTAGNRLYMVGHQDGGFPDLGWHITGEMGGIWDHPIKLMDGFTAAVSINQKQICL-DKA 120 Query: 73 DGFTSEPWGSRFQYDHGLSHIPVAMERTQFAPERVKGMTAAYELHHYGDEDLHLQVELLV 132 D F + P+ ++ Y + + + ER QF P+ +GM Y + + L LQ Sbjct: 121 DTFINYPFANKHLYQQVANGLDI--ERLQFVPDNKEGMVVEYTFINKQAKPLTLQFTWTG 178 Query: 133 RTDLRPVWFSDEVGVHDGVRDEPTVLNPREVLVQDEGNPWFTLVGTDLAGEELEVRSDLY 192 TDLRP W + + D + L +D+ N WF + G ++ + Sbjct: 179 HTDLRPTWLGERTNMVDAPDAATWTAKSQSWLAKDQKNDWFVMFGANVPASQHHQNKPC- 237 Query: 193 GPEF-TAGAGCGIAFRTELTMAAGERRRFHLFVAGSCTSREECEDSYRXXXXXXXXXXXX 251 EF +AG GCG + +T+ A + + + +AGS S E ++ Sbjct: 238 --EFVSAGRGCGASLVYTITVPANGKTQLPITIAGSYKSAEMATRTFSDVQKNTYVYLAS 295 Query: 252 KRGVYSAVAERAKLTVEGEPLWNEAFKWAKWNNQWLIQQVDGTGRGLTAGGPTYPWWFGC 311 K+ Y+ + + A + V + L +AF+W K+N WLI++V GRGL+AG P YPWWFG Sbjct: 296 KKSRYATIDQLASVQVPDKKL-EQAFRWVKYNTDWLIREVPEIGRGLSAGLPDYPWWFGA 354 Query: 312 DSTYALQGVLAIGDPQLAKDTLNLLLEKSEEVNGNGRFIHELTTLGAVSNRGNTQETAHY 371 D Y L G++ G QL D+++LL + SE+ NGNGR IHE++T GAV N GN E + Sbjct: 355 DGCYTLTGLITTGHKQLVYDSVDLLNKISEKENGNGRIIHEVSTNGAVYNPGNLNEVPQF 414 Query: 372 IAFVWELFRWTGDEELLRRHYGYCVKGIGWLLGEMDPDGDLFPSGYGIIEIAGLNMELID 431 + + E++RWTGD+ LR+++ KG+ WLL E D D +L P G+G++EI G+N E+ID Sbjct: 415 ASMIAEVYRWTGDKGFLRKYFPTVKKGMTWLLAENDKDKNLLPDGFGMMEIHGMNSEMID 474 Query: 432 SAVYTAKALQAMAEMSGYLQEAEAQQKYSELAQRCTAAVNRSFWQENEGLF 482 AVYT +A A M+ L + Y A +N FW G + Sbjct: 475 VAVYTQEAFANAAYMASELGDKTLAMDYQRKADNLKTKINTDFWVPESGSY 525 >Q1AZ91.1 Q1AZ91_RUBXD Glycogen debranching enzyme-like protein Length = 869 Score = 151 bits (382), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 146/549 (26%), Positives = 216/549 (39%), Gaps = 104/549 (18%) Query: 13 YYNGGEYVKICGTQDGYFPDFGHHTANEMGGIWLHPIKLLDGFWLKLTDTKRNISVWSRA 72 Y GE + GT+DG FP G HT EMGG+W PIKLLDG W Sbjct: 49 YVTTGERAYVVGTEDGRFPAMGFHTRGEMGGVWSPPIKLLDGIWF--------------- 93 Query: 73 DGFTSE--PWGSRFQYDHGLSHIPV------AMERTQFAPERVKGMTAAYELHHYGDEDL 124 G E P +RF +G + + A+ RT F P+ ++ ++ L Sbjct: 94 -GVDGEWLPPATRFTSGYGYVRMELPGREGLAVTRTDFVPDGLRAALFGLTFSAETEQSL 152 Query: 125 HLQV----ELL-------VRTDLRPVWFSDEVGVHDGV-----RDEPTVLNPREVLVQDE 168 L+V EL+ + D V V G R P N L D Sbjct: 153 TLKVDAHSELMGAYPWGETTPSQKTYNLEDSVSVEHGRLVFRERGTPPAEN---ALPHD- 208 Query: 169 GNPWFTLVGTDLAGEELEVRSDLYGPEFTA-------------------GAGCGIAFRTE 209 W +VG+ L + D GP+ G G G R E Sbjct: 209 ---WAAVVGSSLEPSGSDTGEDFRGPQDPPVICPASPEEAPDRCDDTAYGKGKGGQLRYE 265 Query: 210 LTMAAGERRRFHLFVAGS-----CTSREECEDSYRXXXXXXXXXXXXKRGVYS------- 257 +T+ AGE R VAG+ + E ED+ + Sbjct: 266 ITVPAGETRTVWFAVAGADFDGEASDGEAPEDAVAAAIEEHEAALADPEALLEEKVSGRL 325 Query: 258 AVAERAKLTVEGEPLWNEAFKWAKWNNQWLIQQVDG--------------------TGRG 297 A+A L + G+ +W+K N +Q + R Sbjct: 326 ALARYTSLDLPGDRRLERGIEWSKQNLADSVQAAEDLEIRETNAGQSYPAPEGELDRARF 385 Query: 298 LTAGGPTYPWWFGCDSTYALQGVLAIGDPQLAKDTLNLLLEKSEEVNG-NGRFIHELTTL 356 L AG P Y W F D Y +A G + KD L L + SE NG +G+ +HE+ T Sbjct: 386 LGAGFPDYQWLFATDGEYTAFASVAAGQFEPIKDHLRALRDVSEIDNGGSGKVVHEVVTD 445 Query: 357 GAV-----SNRGNTQETAHYIAFVWELFRWTGDEELLRRHYGYCVKGIGWLLGEMDPDGD 411 G+V ++ GNT ETA + + V ++RWTGD+ YG+ + + ++ E+D DGD Sbjct: 446 GSVYFGSNADPGNTDETAKFPSAVALVWRWTGDDAFRDEMYGFARRNMHYIFRELDEDGD 505 Query: 412 LFPSGYGIIEIAGLNMELIDSAVYTAKALQAMAEMSGYLQEAEAQQKYSELAQRCTAAVN 471 L+P G G +E G+ E +D+AVYT + L +A+M+ + E ++ + + Sbjct: 506 LWPEGLGNVEREGMGEEKLDNAVYTIRGLYDLADMARSRGDEETERWAVKRTRAMERRFE 565 Query: 472 RSFWQENEG 480 R++W EG Sbjct: 566 RAWWFGREG 574 >A5UU77.1 A5UU77_ROSS1 Amylo-alpha-1,6-glucosidase Length = 676 Score = 104 bits (259), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 61/185 (32%), Positives = 93/185 (50%), Gaps = 4/185 (2%) Query: 298 LTAGGPTYPWWFGCDSTYALQGVLAIGDPQLAKDTLNLLLEKSEEVNGNGRFIHELTTLG 357 L AG P YP FGCD+TY++ GV+A G A+ L L + R HE+TT G Sbjct: 284 LLAGLPEYPQLFGCDTTYSVPGVVASGFASTARSALEELGRYAW--RACSRVPHEITTNG 341 Query: 358 AVSNRGNTQETAHYIAFVWELFRWTGDEELLRRHYGYCVKGIGWLLGEMDPDGDLFPSGY 417 V + GN QET + W+ RW+GD + L R Y CV+G+ ++ G +P G Sbjct: 342 RVYHPGNIQETPQFAVACWDYVRWSGDLDFLERMYPLCVEGLEHFSATLEGRG--YPIGD 399 Query: 418 GIIEIAGLNMELIDSAVYTAKALQAMAEMSGYLQEAEAQQKYSELAQRCTAAVNRSFWQE 477 G++E+ G+ +D Y +AL + +M+ L +++ A TA +W E Sbjct: 400 GVVEVPGMGACKLDVVCYLYQALTTLRDMALTLGRPGDAVRFAGYAGELTARFESDWWIE 459 Query: 478 NEGLF 482 EG++ Sbjct: 460 QEGMY 464 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,387,794,484 Number of extensions: 149592270 Number of successful extensions: 263320 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 263315 Number of HSP's successfully gapped: 17 Length of query: 554 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 413 Effective length of database: 1,732,333,088 Effective search space: 715453565344 Effective search space used: 715453565344 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)