BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0005.p (372 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5D3G1.1 C5D3G1_9BACI Spore germination protein 256 2e-66 A7GUP5.1 A7GUP5_BACCN Spore germination protein 236 3e-60 C3HC12.1 C3HC12_BACTU Spore germination protein 224 8e-57 C3HTF9.1 C3HTF9_BACTU Spore germination protein 224 1e-56 Q4V1W1.1 Q4V1W1_BACCZ Spore germination protein 213 2e-53 >C5D3G1.1 C5D3G1_9BACI Spore germination protein Length = 370 Score = 256 bits (654), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 132/370 (35%), Positives = 223/370 (60%), Gaps = 4/370 (1%) Query: 1 MIEKGKISAAQLGLLFYSVTAYDGILYIPKVTGKEAGHDLWLSPIWAHXXXXXXXXXXXX 60 MIEKGKISAAQ+G++ Y IL +P +T + A D+WLSP+WA Sbjct: 1 MIEKGKISAAQMGMMMYPTIIATAILLVPAITARHAKQDMWLSPLWASLIGFLIVHIAVQ 60 Query: 61 XSSRFPKETIIQYSVRLLGPWAGKAVGLVVILYGINLTSVILREFGDFISAVFLRETPPL 120 +P++T+I+YS +LG + GK VGL+ + + ++++ +I+RE+G+F+ FL TP Sbjct: 61 LHQLYPEKTLIEYSRDILGKFLGKVVGLIFLFFHLHISGIIVREYGEFVVGNFLFHTPLF 120 Query: 121 IAIGGIVLLVAYAVRGGLEVLGRLAELFLPITFLVFGLLIILSIPEWDVNNILPVMGEGI 180 +G ++L+ ++AVRGG+EV+GRLAE+ +PI ++ + IIL IP+ + N+ P+M +GI Sbjct: 121 FIMGSMILVCSFAVRGGVEVIGRLAEMIVPIVIILIVVNIILLIPDMEAKNMFPIMEKGI 180 Query: 181 APSIKGALVPFSWFSGYLMLGLYFPFVSNQKKTTVCIVLTWFALMVTLSVSGLVSVFLFG 240 PS+ GA+VP WFS +L++ P+V+++ K +++ A+M+TLS + +F+ G Sbjct: 181 MPSLMGAMVPQGWFSEFLLIAFLLPYVADRNKGKKWGMISVTAVMLTLSFINIAILFILG 240 Query: 241 KHVSTLNYPFIEVVRYIGIGKFIQHVDALLLIVWLPGTFIQLTTYLYTAALGASQWFGLK 300 YP + VRYI I F++H++A+++ +W+ G F+++ + Y LG +QW L Sbjct: 241 GITDRFTYPGMSAVRYISIADFLEHLEAVVMALWVSGVFLKIAVFYYALVLGTAQWLNLS 300 Query: 301 NYRQLVFPVGFIALVLSMWRTSGVEEFQIYLGTSHVIFDFFNIALG---LLLFLTAWIRS 357 +YR +VFP+G + L+ ++W + E +LG+S F +I G LLLF+ W + Sbjct: 301 DYRPIVFPMGLLLLLFAIWSAPNLMELIHFLGSSSP-FYLMSIQTGLPLLLLFIAKWRKR 359 Query: 358 RKGRRQAVAE 367 Q + Sbjct: 360 TNNPNQVKCQ 369 >A7GUP5.1 A7GUP5_BACCN Spore germination protein Length = 365 Score = 236 bits (602), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 127/368 (34%), Positives = 211/368 (57%), Gaps = 4/368 (1%) Query: 1 MIEKGKISAAQLGLLFYSVTAYDGILYIPKVTGKEAGHDLWLSPIWAHXXXXXXXXXXXX 60 M+EKGKISA Q+ L+ +L +P +T + A D+W+SPIWA Sbjct: 1 MVEKGKISAFQMALIIIPTIIATAVLIVPAITSEHAERDIWISPIWASLNGFFTVFVVYQ 60 Query: 61 XSSRFPKETIIQYSVRLLGPWAGKAVGLVVILYGINLTSVILREFGDFISAVFLRETPPL 120 +P+++IIQYS ++G GK +GLV + + +++ +I RE+ DF+ FL +TP + Sbjct: 61 LHKCYPEKSIIQYSKYIVGRIPGKILGLVYLFFILHICGIITREYADFVIGSFLPKTPII 120 Query: 121 IAIGGIVLLVAYAVRGGLEVLGRLAELFLPITFLVFGLLIILSIPEWDVNNILPVMGEGI 180 + IG ++L+ +AVRGG+EVLGR ++LF+P+ FL +L L IP+ + N+ P+M G+ Sbjct: 121 VVIGSMILICGFAVRGGIEVLGRASQLFVPL-FLFPPVLFFLLIPDLKIENMFPIMEHGV 179 Query: 181 APSIKGALVPFSWFSGYLMLGLYFPFVSNQKKTTVCIVLTWFALMVTLSVSGLVSVFLFG 240 PSI G+ VP +WFS ++ + PFV++ +K +++ F M L + ++ V LFG Sbjct: 180 LPSILGSTVPQAWFSEVSLISMLLPFVTDCEKGRKWGIISVFVAMFILLYANIIGVLLFG 239 Query: 241 KHVSTLNYPFIEVVRYIGIGKFIQHVDALLLIVWLPGTFIQLTTYLYTAALGASQWFGLK 300 V + YP RYI + F +H++++++ +W+ G FI+++ + Y LG +QW L Sbjct: 240 GTVISHIYPVFTAFRYISVATFFEHLESIVITLWVLGAFIKISVFYYVLVLGTAQWLHLS 299 Query: 301 NYRQLVFPVGFIALVLSMWRTSGVEEFQIYLGT-SHVIFDFFNIALGLLLFLTAWIRSRK 359 +YR +VFP+GF +++ +W S +E +LG S + +L L +I RK Sbjct: 300 DYRPIVFPLGFFVILIGVWAFSSGQEMTRFLGNISPFYIPCMMTLMPTVLLLITFI--RK 357 Query: 360 GRRQAVAE 367 R V E Sbjct: 358 KRSYTVKE 365 >C3HC12.1 C3HC12_BACTU Spore germination protein Length = 367 Score = 224 bits (572), Expect = 8e-57, Method: Compositional matrix adjust. Identities = 122/363 (33%), Positives = 206/363 (56%), Gaps = 1/363 (0%) Query: 2 IEKGKISAAQLGLLFYSVTAYDGILYIPKVTGKEAGHDLWLSPIWAHXXXXXXXXXXXXX 61 +EKG+IS+ Q+ + IL +P +TGK AG D+W+S A Sbjct: 1 MEKGRISSFQMACMIVPTIIATAILIVPAITGKFAGRDMWISSTLASVNGFFTVFILYRL 60 Query: 62 SSRFPKETIIQYSVRLLGPWAGKAVGLVVILYGINLTSVILREFGDFISAVFLRETPPLI 121 FP +T +QYS ++G GK +G +L+ +++ I RE+ DF+ FL +TP ++ Sbjct: 61 HRIFPNKTFVQYSECIIGRIPGKILGFAYLLFFLHVAGSICREYADFVIGSFLPKTPMIV 120 Query: 122 AIGGIVLLVAYAVRGGLEVLGRLAELFLPITFLVFGLLIILSIPEWDVNNILPVMGEGIA 181 +G IV++ A+A+RGG+EVLGR A+L L F++ L+IL +PE V NI P+M G+ Sbjct: 121 VLGSIVIICAFAIRGGVEVLGRAAQL-LVPLFILPLFLLILILPELKVVNIFPIMEHGVM 179 Query: 182 PSIKGALVPFSWFSGYLMLGLYFPFVSNQKKTTVCIVLTWFALMVTLSVSGLVSVFLFGK 241 P I GA VP WF+ M+ + P+V++++K +++ M+ L+ + ++S+F FG+ Sbjct: 180 PVIMGAAVPQGWFAEVFMISMLLPYVADRQKGMKWSIISVIVAMLLLTYTNIISIFHFGE 239 Query: 242 HVSTLNYPFIEVVRYIGIGKFIQHVDALLLIVWLPGTFIQLTTYLYTAALGASQWFGLKN 301 V++ +YP V RYI G F +H++++++ W+ G F++++ + Y LG +QW Sbjct: 240 SVTSYSYPVFSVFRYISFGSFFEHLESVVITFWILGIFVKISVFYYALTLGIAQWLQCYE 299 Query: 302 YRQLVFPVGFIALVLSMWRTSGVEEFQIYLGTSHVIFDFFNIALGLLLFLTAWIRSRKGR 361 YR +VFPVGF+ + MW S +E +LG + F + L + L I +K + Sbjct: 300 YRPIVFPVGFLLTLFGMWAASSEQEMTQFLGNVFPFYGFLMLTLIPTILLVITILQKKWK 359 Query: 362 RQA 364 R++ Sbjct: 360 RKS 362 >C3HTF9.1 C3HTF9_BACTU Spore germination protein Length = 367 Score = 224 bits (571), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 122/363 (33%), Positives = 206/363 (56%), Gaps = 1/363 (0%) Query: 2 IEKGKISAAQLGLLFYSVTAYDGILYIPKVTGKEAGHDLWLSPIWAHXXXXXXXXXXXXX 61 +EKG+IS+ Q+ + IL +P +TGK AG D+W+S A Sbjct: 1 MEKGRISSFQMACMIVPTIIATAILIVPAITGKFAGRDMWISSTLASVNGFFTVFILYRL 60 Query: 62 SSRFPKETIIQYSVRLLGPWAGKAVGLVVILYGINLTSVILREFGDFISAVFLRETPPLI 121 FP +T +QYS ++G GK +G +L+ +++ I RE+ DF+ FL +TP ++ Sbjct: 61 HRIFPNKTFVQYSECIIGRIPGKILGFAYLLFFLHVAGSICREYADFVIGSFLPKTPMIV 120 Query: 122 AIGGIVLLVAYAVRGGLEVLGRLAELFLPITFLVFGLLIILSIPEWDVNNILPVMGEGIA 181 +G IV++ A+A+RGG+EVLGR A+L L F++ L+IL +PE V NI P+M G+ Sbjct: 121 VLGSIVIICAFAIRGGVEVLGRAAQL-LVPLFILPLFLLILILPELKVVNIFPIMEHGVM 179 Query: 182 PSIKGALVPFSWFSGYLMLGLYFPFVSNQKKTTVCIVLTWFALMVTLSVSGLVSVFLFGK 241 P I GA VP WF+ M+ + P+V++++K +++ M+ L+ + ++S+F FG+ Sbjct: 180 PVIMGAAVPQGWFAEVFMISMLLPYVADRQKGMKWSIISVIVAMLLLTYTNIISIFHFGE 239 Query: 242 HVSTLNYPFIEVVRYIGIGKFIQHVDALLLIVWLPGTFIQLTTYLYTAALGASQWFGLKN 301 V++ +YP V RYI G F +H++++++ W+ G F++++ + Y LG +QW Sbjct: 240 SVTSYSYPVFSVFRYISFGSFFEHLESVVITFWILGIFVKISVFYYALTLGIAQWLQCYE 299 Query: 302 YRQLVFPVGFIALVLSMWRTSGVEEFQIYLGTSHVIFDFFNIALGLLLFLTAWIRSRKGR 361 YR +VFPVGF+ + MW S +E +LG + F + L + L I +K + Sbjct: 300 YRPIVFPVGFLLTLFGMWVASSEQEMTQFLGNVFPFYGFLMLTLIPTILLVITILQKKWK 359 Query: 362 RQA 364 R++ Sbjct: 360 RKS 362 >Q4V1W1.1 Q4V1W1_BACCZ Spore germination protein Length = 365 Score = 213 bits (543), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 126/359 (35%), Positives = 206/359 (57%), Gaps = 2/359 (0%) Query: 1 MIEKGKISAAQLGLLFYSVTAYDGILYIPKVTGKEAGHDLWLSPIWAHXXXXXXXXXXXX 60 MIE+GKISA Q+ L+ L +P ++ K AG D+W+SP+ A Sbjct: 1 MIERGKISALQMALMIIPTIIATAALIVPAISSKYAGRDVWISPVLASLNGFFTVFIVYQ 60 Query: 61 XSSRFPKETIIQYSVRLLGPWAGKAVGLVVILYGINLTSVILREFGDFISAVFLRETPPL 120 +PKETIIQYS ++G GK VG + + + +++ V RE+ DFI FL +TP + Sbjct: 61 LHKCYPKETIIQYSRHIVGQIPGKIVGFIYLFFVLHMCGVTTREYADFIIGSFLPKTPMI 120 Query: 121 IAIGGIVLLVAYAVRGGLEVLGRLAELFLPITFLVFGLLIILSIPEWDVNNILPVMGEGI 180 + IG ++++ A+AVRGG+EVLGR+A+L + FL+ LL +L IP+ + N+ P+M G+ Sbjct: 121 VVIGSVIIVCAFAVRGGVEVLGRVAQLLV-PLFLLPPLLFVLLIPDLKIENMFPIMEHGM 179 Query: 181 APSIKGALVPFSWFSGYLMLGLYFPFVSNQKKTTVCIVLTWFALMVTLSVSGLVSVFLFG 240 PSI GA VP +W S ++ + PFV + +K +++ +M+ L ++SV LFG Sbjct: 180 MPSILGAAVPQAWISEVSLISMLLPFVIDHEKGRKWGIISVIVVMLILVYINIISVLLFG 239 Query: 241 KHVSTLNYPFIEVVRYIGIGKFIQHVDALLLIVWLPGTFIQLTTYLYTAALGASQWFGLK 300 V+ YP RYI + F +H++++++ +W+ G FI+++ Y LG +QW L Sbjct: 240 GTVNNHIYPVFTAFRYISVATFFEHLESIVITLWVLGAFIKISVLYYALVLGIAQWLHLS 299 Query: 301 NYRQLVFPVGFIALVLSMWRTSGVEEFQIYLGT-SHVIFDFFNIALGLLLFLTAWIRSR 358 +YR +VFP+GF+ L+ +W + +E +LG+ S + +L L A+IR + Sbjct: 300 DYRPIVFPLGFLVLLFGIWASYNGQEMAQFLGSISPFYIPCIMTLIPTMLLLIAFIRKK 358 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.145 0.450 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,146,432,580 Number of extensions: 87288937 Number of successful extensions: 263718 Number of sequences better than 1.0e-05: 649 Number of HSP's gapped: 263058 Number of HSP's successfully gapped: 649 Length of query: 372 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 235 Effective length of database: 1,770,017,148 Effective search space: 415954029780 Effective search space used: 415954029780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)