BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0004.p (401 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5D3G0.1 C5D3G0_9BACI Germination protein, Ger(X)C family 191 9e-47 Q4V1V9.1 Q4V1V9_BACCZ Spore germination protein KC 162 8e-38 A7GUP7.1 A7GUP7_BACCN Spore germination B3 GerAC family protein 151 9e-35 Q9KBJ2.1 Q9KBJ2_BACHD Spore germination protein KC 140 2e-31 B1HQ82.1 B1HQ82_LYSSC Spore germination protein KC 134 2e-29 >C5D3G0.1 C5D3G0_9BACI Germination protein, Ger(X)C family Length = 397 Score = 191 bits (486), Expect = 9e-47, Method: Compositional matrix adjust. Identities = 121/389 (31%), Positives = 203/389 (52%), Gaps = 17/389 (4%) Query: 19 LVMAATVLLFSTGCWDRKEVNDLAIVTTAGLDLKNNGEIELTLEIVHPKEVGGDTQQXXX 78 +V A L+ +GCWDR EVNDLA++ G+D NGEI +T+EIV PK +GG Q Sbjct: 10 IVFACVHLILLSGCWDRTEVNDLALIVGLGIDQTKNGEIRITVEIVVPKSIGGGGQMMGG 69 Query: 79 XXXXXXXXXXXGSTLIWXXXXXXXXXXXXELQVKLSRTVYWGQLEILVIGEALSRSQLRE 138 T+I LQ KL R V+WG +++V GE +++ +R+ Sbjct: 70 GGAGG------SETIIRSGTGVTVADAISNLQEKLPRRVFWGHTKVIVFGEKAAKAGIRQ 123 Query: 139 QLDYLVRDSNVRLRVRPFVCKGTARDFLASDPPLEKTKADFLG--GESKRLFRRAITLNS 196 LD+L R+ VRLR V +GTA+ L PP+E++ ++ L ES+ L R +T+ Sbjct: 124 HLDFLSRNPEVRLRSNVLVSEGTAKSVLELVPPIEQSSSEVLRELSESQLLLR--VTVKD 181 Query: 197 LVQDLGNISKEAFLPYVDT--TQDGG---ESVPYVKGYAAFSSYRMAGLIQGESYSGAKW 251 ++Q L + A LP V ++G + + +++ A F +M G I + G W Sbjct: 182 VLQMLSGDAGAAALPMVKIKPPEEGKKELQPIAFIQRTAIFKKDKMIGDIDDQLTRGVLW 241 Query: 252 ILKQVKGDVETVKLERPSSPLISLGVLSSSTRIDPKFAGEIPRMDIVIEAEMSLVQNTTR 311 + ++K T+K + I+ ++ + T + PK+ +M + AE ++ N T+ Sbjct: 242 LRNEIKEANITIK-PKGEKGNITATLIRAHTELIPKYEKGKWKMIVKATAEDDIILNGTK 300 Query: 312 FKTTDSKFIRNVEEAAVDKIRKKAEITIKQAQR-MKADIFGFGEEIERHNPRKWKHIHDQ 370 + K+ + +E+ + K+ T+K+ Q+ MKADIFGF + R PR+W + D+ Sbjct: 301 LDLLNPKYTKMIEKELAKETNKRIRNTLKKVQKEMKADIFGFADTFHRKYPREWNRVKDR 360 Query: 371 WERMYPLVQANVQVKVRIRQTGMNSVPVG 399 WE ++P V+ V+ KV +R+ G+++ P G Sbjct: 361 WEEIFPDVEVVVKTKVYVRRPGIDTAPQG 389 >Q4V1V9.1 Q4V1V9_BACCZ Spore germination protein KC Length = 411 Score = 162 bits (409), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 100/381 (26%), Positives = 182/381 (47%), Gaps = 24/381 (6%) Query: 31 GCWDRKEVNDLAIVTTAGLDLKNNGEIELTLEIVHPKEVG-GDTQQXXXXXXXXXXXXXX 89 GCWDR+EVND A+V +D + G I LT++I+ P+ V G T Sbjct: 34 GCWDRREVNDTALVLGTAIDKEKRGNIRLTIQILIPRAVSSGQTAGGGGGGGGDH----- 88 Query: 90 GSTLIWXXXXXXXXXXXXELQVKLSRTVYWGQLEILVIGEALSRS-QLREQLDYLVRDSN 148 L+ +LQ K R ++WG +I + GE L++ + +Q+D+L+R Sbjct: 89 -QVLVRSAIGENMVDAASKLQTKFPRKIFWGHCKIYIFGEKLAKKGDIHKQIDFLLRHPE 147 Query: 149 VRLRVRPFVCKGTARDFLASDPPLEKTKADFLGGESKRLFRRAITLNSLVQDLGNI---- 204 R R FV G A+D L PPLE+ +G ++L I +N V+D + Sbjct: 148 PRERAYLFVSDGKAKDLLTLQPPLERD----VGEALRKLSEMHIGMNVTVKDFDQMVIGE 203 Query: 205 SKEAFLPYVD-----TTQDGGESVPYVKGYAAFSSYRMAGLIQGESYSGAKWILKQVKGD 259 + A LP + + ++ E++ Y+ G A F +M G I + G W+ + + Sbjct: 204 AGAALLPLITKLPPMSRENKEEAIAYIIGTAIFKKDKMVGRINTKVTRGLLWLRNETQVS 263 Query: 260 VETVKLERPSSPLISLGVLSSSTRIDPKFAGEIPRMDIVIEAEMSLVQNTTRFKTTDSKF 319 TV ++ + ISL T++ P ++ + + +E ++VQ+ + S Sbjct: 264 AVTVSPKKGET--ISLDPTRQKTKLVPSIKNGKWKIFVKVYSEGTVVQDGSHLDIMSSSV 321 Query: 320 IRNVEEAAVDKIRKKAEITIKQAQR-MKADIFGFGEEIERHNPRKWKHIHDQWERMYPLV 378 + +E+ I++ +++KQ ++ M D+FGF + R P++W + +QW+++ P + Sbjct: 322 TKKIEKELEADIKRYINLSLKQVKKGMNVDVFGFADAFHRKYPKEWGKVKNQWDKVLPQL 381 Query: 379 QANVQVKVRIRQTGMNSVPVG 399 + VK R+R+ G+++ P G Sbjct: 382 DVKIDVKTRVRRPGLSTTPAG 402 >A7GUP7.1 A7GUP7_BACCN Spore germination B3 GerAC family protein Length = 402 Score = 151 bits (382), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 99/381 (25%), Positives = 181/381 (47%), Gaps = 23/381 (6%) Query: 30 TGCWDRKEVNDLAIVTTAGLDLKNNGEIELTLEIVHPKEVGGDTQQXXXXXXXXXXXXXX 89 +GCWDR+EVND A+V G+D + I LT++I+ P+ V G + Sbjct: 25 SGCWDRREVNDTALVLGTGIDKEKGKNIRLTVQILVPRAVKGGQSEAGGGSGGGS----- 79 Query: 90 GSTLIWXXXXXXXXXXXXELQVKLSRTVYWGQLEILVIGEALSRS-QLREQLDYLVRDSN 148 L+ +LQ K SR ++WG +I + + ++ + +Q+D+L+R Sbjct: 80 -QILVRSAIGENMADAASKLQAKFSRKLFWGHCKIYIFSKKFAKEGDIHKQIDFLLRHPE 138 Query: 149 VRLRVRPFVCKGTARDFLASDPPLEKTKADFLGGESKRLFRRAITLNSLVQDLGNI---- 204 R R FV G A D L P LE ++G ++L I +N V D + Sbjct: 139 PRERAYLFVSDGEAIDLLKLQPSLE----SYVGEALRKLSELNIGINVTVIDFEQMLISE 194 Query: 205 SKEAFLPYVD-----TTQDGGESVPYVKGYAAFSSYRMAGLIQGESYSGAKWILKQVKGD 259 + A LP + + ++ E++ V G A F +M G + + G W+ +++ Sbjct: 195 AGVALLPLIKKLPPVSRKNKDETMAIVVGTAVFKKDKMIGRMNTKVTRGILWLRNEIQES 254 Query: 260 VETVKLERPSSPLISLGVLSSSTRIDPKFAGEIPRMDIVIEAEMSLVQNTTRFKTTDSKF 319 V T+ ++ S ISL + T++ P ++ +++E ++VQN +RF Sbjct: 255 VVTITPKKGES--ISLDPVRQKTKLLPTIENGKWKIVAKVQSEGTIVQNGSRFDIMSPNI 312 Query: 320 IRNVEEAAVDKIRKKAEITIKQAQR-MKADIFGFGEEIERHNPRKWKHIHDQWERMYPLV 378 ++ VE+ I++ + +KQ ++ M D FGF + R P++W + +W+++ P V Sbjct: 313 VKKVEKELEKDIKQYMNLALKQVKKEMNVDAFGFADAFHRKYPKEWDKVKHRWDKLLPQV 372 Query: 379 QANVQVKVRIRQTGMNSVPVG 399 + +K R+R+ G+++ PVG Sbjct: 373 DVKIVMKTRVRRPGLSTTPVG 393 >Q9KBJ2.1 Q9KBJ2_BACHD Spore germination protein KC Length = 399 Score = 140 bits (353), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 111/404 (27%), Positives = 187/404 (46%), Gaps = 33/404 (8%) Query: 15 KSILLVMAATVLLFSTGCWDRKEVNDLAIVTTAGLDLKNNGEIELTLEIVHPKEVGGDTQ 74 K+ LLV+ LL S+ CWD E++DL+I++ G+D NG +++ +I++P EV D Sbjct: 5 KTQLLVLFFATLLMSS-CWDASELSDLSIISGVGIDKGENGTYKVSFQIINPSEVSADED 63 Query: 75 QXXXXXXXXXXXXXXGSTLIWXXXXXXXXXXXXELQVKLSRTVYWGQLEILVIGEALSRS 134 G+++ + +LSR +Y ++VIGE +R Sbjct: 64 GFQQGSTSTVVISSVGNSIF---------EAVRKASEQLSRRIYSPHTNLVVIGEDFARE 114 Query: 135 Q-LREQLDYLVRDSNVRLRVRPFVCKG-TARDFLASDPPLEKTKADFLG----------G 182 + L + LD+ RD R + + +G TA + L P E+ A + G Sbjct: 115 EGLNKALDWFERDHAFRTNIPMVIARGSTAEEVLLVQSPFERVPAKKIDEAIKTSGAVWG 174 Query: 183 ESKRLFRRAITLNSLVQDLGN-ISKEAFLPYVDTTQDG---GESVPYV----KGYAAFSS 234 E+K + + + +N L N IS + +G G+S P V G A F Sbjct: 175 ETKEVEIQDVIINQLRMGRENTISGIKIVGNFSEAVEGEGLGKSKPPVILQAAGMAMFRD 234 Query: 235 YRMAGLIQGESYSGAKWILKQVKGDVETVKLERPSSPLISLGVLSSSTRIDPKFAG-EIP 293 + + ++ G WI ++ V V L + I++ ++ S+ +++ K E Sbjct: 235 GHLHSWLDHKAARGTNWIDNAIESTV--VTLPCHNDEKIAIEIIQSNAQMEAKMTDQETL 292 Query: 294 RMDIVIEAEMSLVQNTTRFKTTDSKFIRNVEEAAVDKIRKKAEITIKQAQRMKADIFGFG 353 +++ I AE ++ + R T + I VE+ D+IR +A I+ +Q + DIFGFG Sbjct: 293 EINLTIFAEGNIGEVNCRVDLTVIENIHKVEKQLEDEIRSEALEAIQISQEEQIDIFGFG 352 Query: 354 EEIERHNPRKWKHIHDQWERMYPLVQANVQVKVRIRQTGMNSVP 397 EE+ R P +WK + DQW + +Q NVQV IR+TG + P Sbjct: 353 EEVHRKYPDQWKMLKDQWNEHFAELQVNVQVNTFIRRTGFRNNP 396 >B1HQ82.1 B1HQ82_LYSSC Spore germination protein KC Length = 379 Score = 134 bits (336), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 98/383 (25%), Positives = 170/383 (44%), Gaps = 34/383 (8%) Query: 30 TGCWDRKEVNDLAIVTTAGLDLKNNGEIELTLEIVHPKEVGGDTQQXXXXXXXXXXXXXX 89 TGCW+++E+N+LAIVT G+D K + IEL+++IV+P ++ T+ Sbjct: 3 TGCWNKRELNELAIVTAVGVD-KTDEFIELSIQIVNPSQIA--TKNSTSFQVPVVTYHAK 59 Query: 90 GSTLIWXXXXXXXXXXXXELQVKLSRTVYWGQLEILVIGEALSRSQLREQLDYLVRDSNV 149 G TL +L + R Y+ ++++IGE ++ + LD RD Sbjct: 60 GKTLF---------EAIRKLTTQTPRKPYYAHTQVIIIGEEMAEYGMNSILDLFQRDPEG 110 Query: 150 RLRVRPFVC-KGTARDFLASDPPLEKTKADFL-----GGESKRLFRRAITLNSLVQDLGN 203 R V + TA+D L+ PLE A L E ++ L+ L+ LG+ Sbjct: 111 RSDFNIIVAHESTAQDILSILTPLEDIPASKLLKSLKDSEDALGTTESVILDDLINSLGS 170 Query: 204 ISKEAFLPYVDT--TQDGGESVPYVK-----------GYAAFSSYRMAGLIQGESYSGAK 250 I A LP + D G+S ++ G A F +Y++ G + E G Sbjct: 171 IDHSAVLPSIQIHGDADAGKSSSNIEKMDSPANLQYGGLAVFKNYKLIGFLSEEESRGYN 230 Query: 251 WILKQVKGDVETVKLERPSSPLISLGVLSSSTRIDPKFAGEIPRMDIVIEAEMSLVQNTT 310 ++ +KG V+ V IS V +SST++ F + P + + +E E ++ + + Sbjct: 231 FLHNHIKGTVQKVPCSEKGK--ISAEVFNSSTKVTGSFKNDKPSIHVQVELEQNIAEISC 288 Query: 311 RFKTTDSKFIRNVEEAAVDKIRKKAEITIKQAQRM-KADIFGFGEEIERHNPRKWKHIHD 369 T + I + + ++ E T Q+ + DI F E + R + + WK I Sbjct: 289 PLDLTKPETIDTLNKETSKLVQANLEQTFHTIQKTYQVDILKFSEVLHREDHQAWKRIKK 348 Query: 370 QWERMYPLVQANVQVKVRIRQTG 392 W +YP + +++VKV+ + G Sbjct: 349 DWATLYPNLPVHIEVKVKTKGIG 371 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,068,974,140 Number of extensions: 77570187 Number of successful extensions: 191957 Number of sequences better than 1.0e-05: 392 Number of HSP's gapped: 191777 Number of HSP's successfully gapped: 396 Length of query: 401 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 263 Effective length of database: 1,760,596,133 Effective search space: 463036782979 Effective search space used: 463036782979 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)