BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0003.p (511 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q4V1V8.1 Q4V1V8_BACCZ Spore germination protein KA 473 e-131 C3HTG2.1 C3HTG2_BACTU GerA spore germination protein 472 e-131 A7GUP8.1 A7GUP8_BACCN GerA spore germination protein 462 e-128 C5D3G2.1 C5D3G2_9BACI GerA spore germination protein 447 e-123 C3HC09.1 C3HC09_BACTU GerA spore germination protein 442 e-122 >Q4V1V8.1 Q4V1V8_BACCZ Spore germination protein KA Length = 523 Score = 473 bits (1216), Expect = e-131, Method: Compositional matrix adjust. Identities = 231/485 (47%), Positives = 323/485 (66%) Query: 14 KDRTALVPELDLNIEAFKRVLGEADDVSFRPFLIGGTIRAELVNIPSMSNRQEIDNNVLK 73 + ++ L +LD N++ + + + DV FR F IGG +A L+ + +SN +EIDN VL Sbjct: 26 RQQSLLSSDLDGNLKVLRSMYKDCFDVIFRTFWIGGQAKATLIYVEGLSNVEEIDNYVLT 85 Query: 74 PLMQSGDESSNDLQSVKMRLLPVMSADEAGTIEECAGQLIKGYPVLLVDGCAGALLLGLV 133 PLM +E ++ L + + + + E T +C + G PVLLV L LGL Sbjct: 86 PLMSETEEIAHSLHEMLEKKIHISKIKEVKTFADCIENISIGNPVLLVQQEMSGLALGLP 145 Query: 134 QWDKRSIEEPQTETSLRGPREGFTESITTNLSQLRRRIRSVKLQVQSYSIGSYTGTEVCI 193 +W+KRSIEEP+ E+ +RGPREGF E++ N + LR++I+S +++S IG YT T + I Sbjct: 146 KWEKRSIEEPEAESVVRGPREGFVETLGVNTALLRKKIKSPDFKMKSIKIGRYTDTSIVI 205 Query: 194 AYIDGLAKPSLLHEVQSRLERINIDSVLETGYIEELTEDNPYSPFPQQQFTERVDVAAGS 253 AYI+G+A +L+ EVQ+RL+RI ID VLE+GYIEEL EDNPYSPFPQ TER DVAA + Sbjct: 206 AYIEGIADQTLIEEVQNRLQRIEIDGVLESGYIEELIEDNPYSPFPQLLNTERPDVAAAN 265 Query: 254 LLEGRIVLLVDGTPDVLIVPXXXXXXXXXXDDYYNRTVYSSFLRFLRYWTLMLSMILPAV 313 LLEGR+++LVDGTP VLI P +DYY R + SS +R LR+ ++ S++LP++ Sbjct: 266 LLEGRVIILVDGTPFVLIAPISFFSLLQSPEDYYQRFLISSAIRLLRFVFMVFSLVLPSL 325 Query: 314 YVAILNFHHEMVPGKLLASIASAREEIPFPTFVEVMMMQLAFEVLREAGLRLPRQIGSAV 373 YVAIL +H EMVP LL S AS+RE +PFP VE +MM++ FE LREAG+RLP+Q+G+AV Sbjct: 326 YVAILTYHQEMVPTSLLISAASSREAVPFPALVEALMMEVTFEALREAGVRLPKQVGAAV 385 Query: 374 TIVGALVVGEAAVSAGLVSAPIVIIIAFTGIAGFTAPHYSLEFAIRXXXXXXXXXXXXXX 433 +IVGALV+G+AAV AGLVSAP+VI++A TG++ F PHY+ A+R Sbjct: 386 SIVGALVIGQAAVQAGLVSAPMVIVVAITGVSSFMVPHYTQGIALRMLRFPIMFLAGSLG 445 Query: 434 XXXXXXXXIAIAIHLCMLRSYGVPFMSPFAPFVPNEMKDTMIRAPLWKMSRRPGFSSLQN 493 I I +HLC LRS+G+P+++P AP E+KDT+IRAP W M+ RP +S + Sbjct: 446 LLGVMLGMITIVVHLCTLRSFGIPYLTPIAPMKGRELKDTLIRAPWWMMNTRPRLTSEYS 505 Query: 494 RHRLA 498 ++R A Sbjct: 506 QYRQA 510 >C3HTG2.1 C3HTG2_BACTU GerA spore germination protein Length = 505 Score = 472 bits (1215), Expect = e-131, Method: Compositional matrix adjust. Identities = 235/478 (49%), Positives = 323/478 (67%), Gaps = 6/478 (1%) Query: 22 ELDLNIEAFKRVLGEADDVSFRPFLIGGTIRAELVNIPSMSNRQEIDNNVLKPLMQSGDE 81 ++D NI+ + + DV+FR FLI G I+A LV I +S+ +E++ VL PLM E Sbjct: 16 DVDENIKLLRSMYKNCFDVTFRTFLINGQIKAVLVYIEGLSSVEEVECYVLAPLMA---E 72 Query: 82 SSNDLQSVKMRL---LPVMSADEAGTIEECAGQLIKGYPVLLVDGCAGALLLGLVQWDKR 138 ++N + S + L + V E TIE+C + G P++L++ L GL +W+KR Sbjct: 73 TANGIHSPRELLEKKIHVSKIKEIKTIEDCMENISNGNPIVLIEQEIQGLAFGLAKWEKR 132 Query: 139 SIEEPQTETSLRGPREGFTESITTNLSQLRRRIRSVKLQVQSYSIGSYTGTEVCIAYIDG 198 SIEEPQ E +RGPREGF E++ N S LRR+I+S +++S IG +T T + IAYI+G Sbjct: 133 SIEEPQAEGVVRGPREGFVETLGVNTSLLRRKIKSPNFKMKSIKIGRFTDTNLVIAYIEG 192 Query: 199 LAKPSLLHEVQSRLERINIDSVLETGYIEELTEDNPYSPFPQQQFTERVDVAAGSLLEGR 258 ++ P+L+ EV++RL RINIDSVLE+GYIEEL EDNPYSPFPQ TER DVAA +LLEGR Sbjct: 193 ISDPTLIQEVENRLLRINIDSVLESGYIEELIEDNPYSPFPQLVNTERPDVAAANLLEGR 252 Query: 259 IVLLVDGTPDVLIVPXXXXXXXXXXDDYYNRTVYSSFLRFLRYWTLMLSMILPAVYVAIL 318 +V+LVDGTP VLI P +DYY R + S +R LR+ ++ S++LP++YVA+L Sbjct: 253 VVILVDGTPSVLIAPISFFTLLQSPEDYYQRFLIGSVIRMLRFVFMIFSLLLPSLYVAVL 312 Query: 319 NFHHEMVPGKLLASIASAREEIPFPTFVEVMMMQLAFEVLREAGLRLPRQIGSAVTIVGA 378 +H EMVP LL S+AS+RE +PFP VE +MM++ FE LREAG+RLP+Q+G+AV+IVGA Sbjct: 313 TYHQEMVPTALLISMASSREGVPFPALVEALMMEITFEALREAGIRLPKQVGAAVSIVGA 372 Query: 379 LVVGEAAVSAGLVSAPIVIIIAFTGIAGFTAPHYSLEFAIRXXXXXXXXXXXXXXXXXXX 438 LV+G+AAV AGLVSAP+VI++A TG++ F PHY+ A+R Sbjct: 373 LVIGQAAVQAGLVSAPMVIVVAITGVSSFMVPHYTQGIALRMLRFPIIFLAGSLGLLGVM 432 Query: 439 XXXIAIAIHLCMLRSYGVPFMSPFAPFVPNEMKDTMIRAPLWKMSRRPGFSSLQNRHR 496 IAI +HLC LRS+GVPF++P AP E+KDT+IRAP WKM+ RP + N++R Sbjct: 433 LGVIAIVVHLCALRSFGVPFLTPIAPLKGLELKDTLIRAPWWKMNTRPHLTGEFNKYR 490 >A7GUP8.1 A7GUP8_BACCN GerA spore germination protein Length = 510 Score = 462 bits (1189), Expect = e-128, Method: Compositional matrix adjust. Identities = 229/483 (47%), Positives = 316/483 (65%) Query: 14 KDRTALVPELDLNIEAFKRVLGEADDVSFRPFLIGGTIRAELVNIPSMSNRQEIDNNVLK 73 + + L +LD N+E + + + DV F FLI G +RA L+ + +SN +EI +VL Sbjct: 13 QQQVLLSSDLDENLEILRSMYQDCFDVVFHMFLIRGQMRAVLIYVEGLSNVEEIHTHVLS 72 Query: 74 PLMQSGDESSNDLQSVKMRLLPVMSADEAGTIEECAGQLIKGYPVLLVDGCAGALLLGLV 133 PLM + + V + + + E T +C + G PVLLV L LGL Sbjct: 73 PLMSEVEGGGLNPLEVLEKQIHLSKTKEVRTFTDCIENISSGNPVLLVHQEMSGLALGLA 132 Query: 134 QWDKRSIEEPQTETSLRGPREGFTESITTNLSQLRRRIRSVKLQVQSYSIGSYTGTEVCI 193 +W+KRSIEEP E +RGPREGF E+++ N S LRRRI+S +++ G YT T V I Sbjct: 133 KWEKRSIEEPVAENVVRGPREGFVETLSVNTSLLRRRIKSPNFKMKLIQTGRYTDTSVVI 192 Query: 194 AYIDGLAKPSLLHEVQSRLERINIDSVLETGYIEELTEDNPYSPFPQQQFTERVDVAAGS 253 AYI+GLA +L+ EV++RL RI+IDS+LE+GYIEEL EDNPYSPFPQ TER DVAA + Sbjct: 193 AYIEGLANETLIEEVENRLRRIDIDSILESGYIEELIEDNPYSPFPQLINTERPDVAAAN 252 Query: 254 LLEGRIVLLVDGTPDVLIVPXXXXXXXXXXDDYYNRTVYSSFLRFLRYWTLMLSMILPAV 313 LLEGR+V+L+DGTP VLI P +DYY R + SS +R LR+ ++ S++LP++ Sbjct: 253 LLEGRVVILMDGTPFVLIAPISFFSLLQSPEDYYQRFLISSAIRLLRFVFMIFSLVLPSI 312 Query: 314 YVAILNFHHEMVPGKLLASIASAREEIPFPTFVEVMMMQLAFEVLREAGLRLPRQIGSAV 373 YVA+L +H EMVP LL S+AS+RE +PFP +E +MM++ FE LREAG+RLP+Q+G+AV Sbjct: 313 YVAVLTYHQEMVPTSLLISMASSRETVPFPALIEALMMEITFEALREAGVRLPKQVGAAV 372 Query: 374 TIVGALVVGEAAVSAGLVSAPIVIIIAFTGIAGFTAPHYSLEFAIRXXXXXXXXXXXXXX 433 +IVGALV+G+AAV AGLVS P++I++A TGI+ F PHY+ A+R Sbjct: 373 SIVGALVIGQAAVQAGLVSTPMIIVVAITGISSFMVPHYTQGIALRMLRFPIMFLAGSLG 432 Query: 434 XXXXXXXXIAIAIHLCMLRSYGVPFMSPFAPFVPNEMKDTMIRAPLWKMSRRPGFSSLQN 493 IAI IHLC LRS+ +P+++P AP +E+KDT+IR P WKM+ RP ++ N Sbjct: 433 LLGVMLGVIAIVIHLCALRSFDIPYLTPIAPMNAHELKDTLIRVPWWKMNTRPHLTNEFN 492 Query: 494 RHR 496 R+R Sbjct: 493 RYR 495 >C5D3G2.1 C5D3G2_9BACI GerA spore germination protein Length = 541 Score = 447 bits (1150), Expect = e-123, Method: Compositional matrix adjust. Identities = 228/480 (47%), Positives = 315/480 (65%), Gaps = 5/480 (1%) Query: 19 LVPELDLNIEAFKRVLGEADDVSFRPFLIGGTIRAELVNIPSMSNRQEIDNNVLKPLMQS 78 L +L+ N++ + + DV FRPF IGG +A L+ I +++ +EID VL PLM+ Sbjct: 34 LSTDLNKNLQKLQNIYQNCMDVVFRPFQIGGKTKAALIYIDGLADIKEIDAGVLTPLMKE 93 Query: 79 GDESSNDLQSVKM-----RLLPVMSADEAGTIEECAGQLIKGYPVLLVDGCAGALLLGLV 133 + N Q + + + L V TI EC Q+ G PVLL+D + LGL Sbjct: 94 NNAKENTDQFIHVNHILEQKLTVSKVKRVQTITECIEQISGGNPVLLIDQQSEGFALGLA 153 Query: 134 QWDKRSIEEPQTETSLRGPREGFTESITTNLSQLRRRIRSVKLQVQSYSIGSYTGTEVCI 193 W+KRSIEEP E+ +RGPREGFTES+ TNL+ LRR+IRS +L+ +S IG YT TEV I Sbjct: 154 HWEKRSIEEPVGESVIRGPREGFTESLGTNLALLRRKIRSPQLKTKSMKIGKYTQTEVVI 213 Query: 194 AYIDGLAKPSLLHEVQSRLERINIDSVLETGYIEELTEDNPYSPFPQQQFTERVDVAAGS 253 +YI+G+A+ +L+ E+++RL RI +D +LET YIEE EDNPYSPFPQ TER DV A + Sbjct: 214 SYIEGIAEQTLIQEIEARLNRIKVDGILETSYIEEFIEDNPYSPFPQVLNTERPDVVAAN 273 Query: 254 LLEGRIVLLVDGTPDVLIVPXXXXXXXXXXDDYYNRTVYSSFLRFLRYWTLMLSMILPAV 313 LLEGR+ + DGTP VLIVP +DYY R++ S+ R+LRY L++S++LP++ Sbjct: 274 LLEGRVAIFQDGTPFVLIVPVSFYSLLQSNEDYYQRSLMSTATRWLRYIFLLISLLLPSL 333 Query: 314 YVAILNFHHEMVPGKLLASIASAREEIPFPTFVEVMMMQLAFEVLREAGLRLPRQIGSAV 373 YVA+L FH EM+P LL +IA++RE++PFP VE ++M+LAFE +REAG+RLP+Q G+AV Sbjct: 334 YVALLTFHQEMLPTTLLITIATSREKVPFPALVEAVLMELAFEAIREAGVRLPKQAGAAV 393 Query: 374 TIVGALVVGEAAVSAGLVSAPIVIIIAFTGIAGFTAPHYSLEFAIRXXXXXXXXXXXXXX 433 +IVGALV+G+AAV AGLVSAP+++++A TGIA FT P Y+ A+R Sbjct: 394 SIVGALVIGQAAVQAGLVSAPLIMVVALTGIASFTFPRYTAGMALRLLRFPMIFLAGTLG 453 Query: 434 XXXXXXXXIAIAIHLCMLRSYGVPFMSPFAPFVPNEMKDTMIRAPLWKMSRRPGFSSLQN 493 + I H+ LRS+GVP++SP AP EMKD ++RAP WK + RP + N Sbjct: 454 LLGIMLGLLMIVTHMATLRSFGVPYLSPMAPIQGGEMKDVLLRAPFWKFNTRPHLTGADN 513 >C3HC09.1 C3HC09_BACTU GerA spore germination protein Length = 448 Score = 442 bits (1137), Expect = e-122, Method: Compositional matrix adjust. Identities = 218/435 (50%), Positives = 297/435 (68%), Gaps = 6/435 (1%) Query: 65 QEIDNNVLKPLMQSGDESSNDLQSVKMRL---LPVMSADEAGTIEECAGQLIKGYPVLLV 121 +E++ VL PLM E++N + S + L + V E TIE+C + G P++L+ Sbjct: 2 EEVECYVLAPLMA---ETANGIHSPRELLEKKIHVSKIKEIKTIEDCMENISNGNPIVLI 58 Query: 122 DGCAGALLLGLVQWDKRSIEEPQTETSLRGPREGFTESITTNLSQLRRRIRSVKLQVQSY 181 + L GL +W+KRSIEEPQ E +RGPREGF E++ N S LRR+I+S +++S Sbjct: 59 EQEIHGLAFGLAKWEKRSIEEPQAEGVVRGPREGFVETLGVNTSLLRRKIKSPNFKMKSI 118 Query: 182 SIGSYTGTEVCIAYIDGLAKPSLLHEVQSRLERINIDSVLETGYIEELTEDNPYSPFPQQ 241 IG +T T + IAYI+G++ P+L+ EV++RL RINIDSVLE+GYIEEL EDNPYSPFPQ Sbjct: 119 KIGRFTDTNLVIAYIEGISDPTLIQEVENRLLRINIDSVLESGYIEELIEDNPYSPFPQL 178 Query: 242 QFTERVDVAAGSLLEGRIVLLVDGTPDVLIVPXXXXXXXXXXDDYYNRTVYSSFLRFLRY 301 TER DVAA +LLEGR+V+LVDGTP VLI P +DYY R + S +R LR+ Sbjct: 179 VNTERPDVAAANLLEGRVVILVDGTPSVLIAPISFFTLLQSPEDYYQRFLIGSVIRMLRF 238 Query: 302 WTLMLSMILPAVYVAILNFHHEMVPGKLLASIASAREEIPFPTFVEVMMMQLAFEVLREA 361 ++ S++LP++YVA+L +H EMVP LL S+AS+RE +PFP VE +MM++ FE LREA Sbjct: 239 VFMIFSLLLPSLYVAVLTYHQEMVPTALLISMASSREGVPFPALVEALMMEITFEALREA 298 Query: 362 GLRLPRQIGSAVTIVGALVVGEAAVSAGLVSAPIVIIIAFTGIAGFTAPHYSLEFAIRXX 421 G+RLP+Q+G+AV+IVGALV+G+AAV AGLVSAP+VI++A TG++ F PHY+ A+R Sbjct: 299 GIRLPKQVGAAVSIVGALVIGQAAVQAGLVSAPMVIVVAITGVSSFMVPHYTQGIALRML 358 Query: 422 XXXXXXXXXXXXXXXXXXXXIAIAIHLCMLRSYGVPFMSPFAPFVPNEMKDTMIRAPLWK 481 IAI +HLC LRS+GVPF++P AP E+KDT+IRAP WK Sbjct: 359 RFPIIFLAGSLGLLGVMLGVIAIVVHLCALRSFGVPFLTPIAPLKGLELKDTLIRAPWWK 418 Query: 482 MSRRPGFSSLQNRHR 496 M+ RP + N++R Sbjct: 419 MNTRPHLTGEFNKYR 433 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,574,390,413 Number of extensions: 97269332 Number of successful extensions: 257302 Number of sequences better than 1.0e-05: 1092 Number of HSP's gapped: 255445 Number of HSP's successfully gapped: 1093 Length of query: 511 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 371 Effective length of database: 1,741,754,103 Effective search space: 646190772213 Effective search space used: 646190772213 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)