BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0002.p (272 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0MLW8.1 B0MLW8_9FIRM Putative uncharacterized protein 318 4e-85 A8SV58.1 A8SV58_9FIRM Putative uncharacterized protein 298 3e-79 B5YCU0.1 B5YCU0_DICT6 Beta-mannanase 236 1e-60 B8DZI4.1 B8DZI4_DICTD Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 236 2e-60 A4XH98.1 A4XH98_CALS8 Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 217 7e-55 >B0MLW8.1 B0MLW8_9FIRM Putative uncharacterized protein Length = 322 Score = 318 bits (814), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 146/272 (53%), Positives = 190/272 (69%), Gaps = 1/272 (0%) Query: 1 VTGKLPALCGFELLAYSPGINYGDAGEACLIEVEENKNTLDNAWDWVLNKRGLITFTWHW 60 VTGK PAL GFELL+YSP INY D + C+ EV EN TL W+W K+GLIT WHW Sbjct: 52 VTGKQPALLGFELLSYSPNINYSDTDDECMTEVMENYGTLKRVWEWA-EKKGLITMCWHW 110 Query: 61 FSPFGSRDKGFYTEYTTFDASRAVVEGTEEHKMLLSDMDHMAALLKPFCEKRIPVLWRPF 120 FSP R K F++E T FDAS+AV++GT E+K LLSDMD MA +L+PFCEK +P+LWRPF Sbjct: 111 FSPLYGRSKSFFSENTDFDASKAVIDGTPENKALLSDMDTMAGILRPFCEKGVPILWRPF 170 Query: 121 HESEGEWFWWGAKGPEVAKQLYRMMYERYTNHHSLNNLIWVWNSPLPEGYVGDDVCDVIS 180 HE +G+WFWWG KG + K+L+R+M++RYT+ L+NLIWVWNS +PE Y GD V D+IS Sbjct: 171 HEGDGDWFWWGKKGADTVKKLFRLMHDRYTDKFHLDNLIWVWNSQVPECYPGDGVVDIIS 230 Query: 181 LDLYPPKHTHTDLEKEYNGLVKITKANKLAALGEIGVLPGIPALSETRIPWVWYMIWSND 240 D+YPP+H HT + Y L +IT K+ +GE G LP A+ ++ W YM WS Sbjct: 231 RDMYPPEHEHTSQSEMYYNLCEITSEKKITIIGETGTLPSPEAVVSEKVGWSSYMTWSKP 290 Query: 241 YGASGEWTTKEELRRAYDHPYAVTLDTLPRLY 272 + + ++ T E+L++ Y+ YAVT DTLP LY Sbjct: 291 FCLTEKFNTFEQLKKVYNSEYAVTKDTLPDLY 322 >A8SV58.1 A8SV58_9FIRM Putative uncharacterized protein Length = 328 Score = 298 bits (764), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 140/263 (53%), Positives = 178/263 (67%) Query: 2 TGKLPALCGFELLAYSPGINYGDAGEACLIEVEENKNTLDNAWDWVLNKRGLITFTWHWF 61 TGK PAL GFELL YSP INY DA CL EV EN++TLD A +W G++TFT+HWF Sbjct: 64 TGKCPALRGFELLGYSPNINYNDASAECLTEVYENQDTLDTALEWAKKTNGIVTFTFHWF 123 Query: 62 SPFGSRDKGFYTEYTTFDASRAVVEGTEEHKMLLSDMDHMAALLKPFCEKRIPVLWRPFH 121 SP G RDK FYTE T F+A + + GT E DMD +A L+ F + IPVLWRPFH Sbjct: 124 SPVGGRDKSFYTENTDFNAEKVFIPGTRERDAFYHDMDIIAKELRRFQLEDIPVLWRPFH 183 Query: 122 ESEGEWFWWGAKGPEVAKQLYRMMYERYTNHHSLNNLIWVWNSPLPEGYVGDDVCDVISL 181 ESEG WFWWGAKGP VA +LYR+M++ YTN ++NNLIWVWN P Y GDD D++S+ Sbjct: 184 ESEGTWFWWGAKGPAVAAKLYRLMFDYYTNVLNINNLIWVWNCPTKGVYPGDDFVDIVSM 243 Query: 182 DLYPPKHTHTDLEKEYNGLVKITKANKLAALGEIGVLPGIPALSETRIPWVWYMIWSNDY 241 D+Y P++T TD +EY LV+ T NK+AAL E+G +P I L ++ PW +YM WS ++ Sbjct: 244 DIYLPEYTATDYSEEYTKLVEATSPNKVAALTEVGYIPDIHLLEKSHTPWAYYMTWSEEF 303 Query: 242 GASGEWTTKEELRRAYDHPYAVT 264 ++ + E L+ YD YAVT Sbjct: 304 CIGEKFNSNENLKAMYDSQYAVT 326 >B5YCU0.1 B5YCU0_DICT6 Beta-mannanase Length = 431 Score = 236 bits (603), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 112/272 (41%), Positives = 171/272 (62%) Query: 1 VTGKLPALCGFELLAYSPGINYGDAGEACLIEVEENKNTLDNAWDWVLNKRGLITFTWHW 60 +TG+ PAL GF+LL+Y+P + +IE+E NK +++ A +W + G++TF WHW Sbjct: 157 ITGEKPALRGFDLLSYTPATKTQEMTPHAIIEIEGNKGSIEKALEWGRDFGGIVTFCWHW 216 Query: 61 FSPFGSRDKGFYTEYTTFDASRAVVEGTEEHKMLLSDMDHMAALLKPFCEKRIPVLWRPF 120 ++P G +DK FYT T FD +A++EGTEE+K LL D+ + L F +P+LWRP Sbjct: 217 YAPLGGKDKTFYTINTDFDLEKALIEGTEENKALLRDIYMIGEKLSIFRGLDLPILWRPL 276 Query: 121 HESEGEWFWWGAKGPEVAKQLYRMMYERYTNHHSLNNLIWVWNSPLPEGYVGDDVCDVIS 180 HE++G WFWWGAKGPE K+LY ++YE T LNNLIW+WN+P P+ + +D+ D+ Sbjct: 277 HEADGRWFWWGAKGPENYKKLYHLLYEILTEKFKLNNLIWIWNAPHPDWEIEEDLYDIAG 336 Query: 181 LDLYPPKHTHTDLEKEYNGLVKITKANKLAALGEIGVLPGIPALSETRIPWVWYMIWSND 240 +D Y P H ++ Y+ L+K+T KL AL E G +P L E ++W+M W D Sbjct: 337 IDNYVPPGNHGPMKFSYDLLLKLTDYRKLVALTENGPIPDPDLLFEYEAYFLWFMPWFGD 396 Query: 241 YGASGEWTTKEELRRAYDHPYAVTLDTLPRLY 272 + + + +KE L++ Y+H +TL+ L +L+ Sbjct: 397 FVFNESFNSKEHLKKVYNHEKVITLNKLRKLF 428 >B8DZI4.1 B8DZI4_DICTD Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 Length = 417 Score = 236 bits (601), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 112/270 (41%), Positives = 169/270 (62%) Query: 2 TGKLPALCGFELLAYSPGINYGDAGEACLIEVEENKNTLDNAWDWVLNKRGLITFTWHWF 61 TGK PAL GF+LL+Y+P + D LIE+E NK +++ A +W + G+IT WHW+ Sbjct: 141 TGKKPALRGFDLLSYTPAVRTQDMTPHALIEIEGNKGSIEKAIEWAKDLGGIITLCWHWY 200 Query: 62 SPFGSRDKGFYTEYTTFDASRAVVEGTEEHKMLLSDMDHMAALLKPFCEKRIPVLWRPFH 121 +P G +DK FYT T F+ A++ GTEE+K LL D++ +A L + IP+LWRP H Sbjct: 201 APLGGKDKTFYTVNTDFNLEEALLNGTEENKTLLRDIEIIAEKLSILRDLDIPILWRPLH 260 Query: 122 ESEGEWFWWGAKGPEVAKQLYRMMYERYTNHHSLNNLIWVWNSPLPEGYVGDDVCDVISL 181 E++G+WFWWGAKGPE K+LY ++YE +T + LNNLIW+WN+P PE + D+ D+ + Sbjct: 261 EADGKWFWWGAKGPENYKKLYMLLYEIFTEKYRLNNLIWIWNAPHPEWKIEKDLYDIAGI 320 Query: 182 DLYPPKHTHTDLEKEYNGLVKITKANKLAALGEIGVLPGIPALSETRIPWVWYMIWSNDY 241 D Y P + ++ Y+ L+++T KL AL E G +P L E ++W+M W D+ Sbjct: 321 DHYAPPGNYGPMKFSYDLLLRLTDNEKLVALTENGPIPDPDLLFEYEAYFLWFMPWFGDF 380 Query: 242 GASGEWTTKEELRRAYDHPYAVTLDTLPRL 271 + + +KE ++ Y+H +TL+ L L Sbjct: 381 VFNENFNSKEHFKKVYNHEKVITLENLKNL 410 >A4XH98.1 A4XH98_CALS8 Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 Length = 452 Score = 217 bits (553), Expect = 7e-55, Method: Compositional matrix adjust. Identities = 107/267 (40%), Positives = 156/267 (58%) Query: 2 TGKLPALCGFELLAYSPGINYGDAGEACLIEVEENKNTLDNAWDWVLNKRGLITFTWHWF 61 TGK L GF+ L+Y+ + + EV NK +++ A W G++TF WHWF Sbjct: 181 TGKKTTLRGFDFLSYTRHTITNNMTYDAMFEVILNKGSVEEAIKWHKELGGIVTFCWHWF 240 Query: 62 SPFGSRDKGFYTEYTTFDASRAVVEGTEEHKMLLSDMDHMAALLKPFCEKRIPVLWRPFH 121 SP G DK FYT+ T FD A+ TEE+K+L+ D+ + LK + +PV++RP H Sbjct: 241 SPIGGNDKTFYTKNTDFDIEVALCPNTEENKLLMEDIYEIGKWLKIMADADVPVIFRPLH 300 Query: 122 ESEGEWFWWGAKGPEVAKQLYRMMYERYTNHHSLNNLIWVWNSPLPEGYVGDDVCDVISL 181 E++G WFWWGAKG + K+LY ++Y+ YTNH LNNLIWVWN+P P+ + D DV + Sbjct: 301 EADGRWFWWGAKGFDAYKKLYYLLYDVYTNHFKLNNLIWVWNAPHPDWRIEMDYYDVAGV 360 Query: 182 DLYPPKHTHTDLEKEYNGLVKITKANKLAALGEIGVLPGIPALSETRIPWVWYMIWSNDY 241 D Y P + L Y+ + ++T+A K AL E G +P L ++ W+W+M W + Sbjct: 361 DFYAPAQNYGPLSFWYDYVYELTEAKKPIALTENGPIPDPDKLQSSKTYWLWFMPWWGGF 420 Query: 242 GASGEWTTKEELRRAYDHPYAVTLDTL 268 G+ + E LR+ Y+HPY +TLD Sbjct: 421 TTDGKINSFEHLRKVYNHPYVITLDKF 447 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.464 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,890,192,285 Number of extensions: 81683798 Number of successful extensions: 181094 Number of sequences better than 1.0e-05: 135 Number of HSP's gapped: 180806 Number of HSP's successfully gapped: 135 Length of query: 272 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 139 Effective length of database: 1,807,701,208 Effective search space: 251270467912 Effective search space used: 251270467912 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)