BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0183.0015.p (205 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B2V0Q6.1 B2V0Q6_CLOBA Intracellular protease, ThiJ/PfpI family 309 1e-82 A6LY46.1 A6LY46_CLOB8 ThiJ/PfpI domain protein 306 9e-82 Q46FK5.1 Q46FK5_METBF Intracellular protease, ThiJ/PfpI family 276 8e-73 C2PNU2.1 C2PNU2_BACCE ThiJ/pfpI 261 3e-68 Q2BBG5.1 Q2BBG5_9BACI Putative uncharacterized protein 261 4e-68 >B2V0Q6.1 B2V0Q6_CLOBA Intracellular protease, ThiJ/PfpI family Length = 208 Score = 309 bits (791), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 142/204 (69%), Positives = 171/204 (83%) Query: 1 MKHTVYLYVFDTMADWEIGYLTAELNSGRYYKKGLAPSTIVTVANEKTLVTTMGGLTIMP 60 MK+TVYLYVFDTMADWEIGYL+ E+NSGRYYKKGL P IVTV KT +TTMGGL I+P Sbjct: 1 MKNTVYLYVFDTMADWEIGYLSTEINSGRYYKKGLMPLKIVTVGITKTPITTMGGLKILP 60 Query: 61 DITVDECSMASAAALILPGGNTWTEAIHNPILQLAERYIQKEILVAAICGATMGLAQAGL 120 +I + ECS+ AALILPGGNTWTEAIH PI+++AE+Y++K I+V AICGAT+GLA G+ Sbjct: 61 EIELKECSVKDTAALILPGGNTWTEAIHAPIIRMAEKYLEKGIVVGAICGATIGLAMGGV 120 Query: 121 LNSRPHTSNDLEYLRMVCPAYTGGDFYQMQSVVTDGTLITASGIAPLEFAVHVLKALDVM 180 LN R HTSNDL YL+MVCP+Y G +Y+ + VVTDG+LITASGIAPLEFA H+LK LDV Sbjct: 121 LNKRAHTSNDLGYLKMVCPSYDGEKYYKQECVVTDGSLITASGIAPLEFAFHILKILDVF 180 Query: 181 TPEKLKAWYSLYKTQEAKYFYELM 204 P+ L +WY+LYKTQE+KYF+ELM Sbjct: 181 LPQTLDSWYNLYKTQESKYFFELM 204 >A6LY46.1 A6LY46_CLOB8 ThiJ/PfpI domain protein Length = 208 Score = 306 bits (783), Expect = 9e-82, Method: Compositional matrix adjust. Identities = 141/204 (69%), Positives = 172/204 (84%) Query: 1 MKHTVYLYVFDTMADWEIGYLTAELNSGRYYKKGLAPSTIVTVANEKTLVTTMGGLTIMP 60 MK+TVYLYVFDTMADWEIGYL AE+NSGRYYKKGL P +VTV K +TTMGGLTI+P Sbjct: 1 MKNTVYLYVFDTMADWEIGYLIAEINSGRYYKKGLMPLKVVTVGITKAPITTMGGLTILP 60 Query: 61 DITVDECSMASAAALILPGGNTWTEAIHNPILQLAERYIQKEILVAAICGATMGLAQAGL 120 +I ++ECS AALILPGGNTWTEAIH PI+++AE+Y++K I+V AICGAT+GLA G+ Sbjct: 61 EIELEECSTHDTAALILPGGNTWTEAIHAPIIRMAEKYLEKGIVVGAICGATIGLAMGGV 120 Query: 121 LNSRPHTSNDLEYLRMVCPAYTGGDFYQMQSVVTDGTLITASGIAPLEFAVHVLKALDVM 180 L+ R HTSNDL YL+MVCP Y G +Y+ + VVTDG+LITASGIAPLEFA+H+LK LDV Sbjct: 121 LDKRDHTSNDLGYLKMVCPNYDGEMYYEQECVVTDGSLITASGIAPLEFALHILKILDVF 180 Query: 181 TPEKLKAWYSLYKTQEAKYFYELM 204 +P+ L +WY+LYKTQE+KYF+ELM Sbjct: 181 SPQTLDSWYNLYKTQESKYFFELM 204 >Q46FK5.1 Q46FK5_METBF Intracellular protease, ThiJ/PfpI family Length = 208 Score = 276 bits (706), Expect = 8e-73, Method: Compositional matrix adjust. Identities = 130/204 (63%), Positives = 161/204 (78%) Query: 1 MKHTVYLYVFDTMADWEIGYLTAELNSGRYYKKGLAPSTIVTVANEKTLVTTMGGLTIMP 60 MK TVYLYVFDTMADWE+GYL+AELNSGR+Y+K P IVTV + T V TMGG+ ++P Sbjct: 1 MKSTVYLYVFDTMADWEVGYLSAELNSGRFYRKESQPLKIVTVGIDNTPVITMGGVKVLP 60 Query: 61 DITVDECSMASAAALILPGGNTWTEAIHNPILQLAERYIQKEILVAAICGATMGLAQAGL 120 DI +E + AAALILPGGNTWTE IH+PIL+ AE ++K ++V AICGATMGLA+ G+ Sbjct: 61 DIGHEELIIEDAAALILPGGNTWTEPIHDPILKKAEECLKKGVIVGAICGATMGLAEKGM 120 Query: 121 LNSRPHTSNDLEYLRMVCPAYTGGDFYQMQSVVTDGTLITASGIAPLEFAVHVLKALDVM 180 LN+R HTSND +L+M+CP YTG +Y+ + VTDG LITASG APLEF VHVLK LDV Sbjct: 121 LNNRWHTSNDPGFLKMMCPNYTGEMYYKQEPTVTDGNLITASGTAPLEFTVHVLKKLDVF 180 Query: 181 TPEKLKAWYSLYKTQEAKYFYELM 204 + E L +WY+LY+T E+KYFYEL+ Sbjct: 181 SLETLDSWYNLYRTHESKYFYELI 204 >C2PNU2.1 C2PNU2_BACCE ThiJ/pfpI Length = 210 Score = 261 bits (667), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 119/201 (59%), Positives = 152/201 (75%) Query: 5 VYLYVFDTMADWEIGYLTAELNSGRYYKKGLAPSTIVTVANEKTLVTTMGGLTIMPDITV 64 V+LYVF+TM+DWE GYL AELNSGRY+KKGL P ++TV K ++TTMGGL+I PDI++ Sbjct: 6 VFLYVFNTMSDWEYGYLIAELNSGRYFKKGLVPLKVITVGANKEMITTMGGLSIKPDISL 65 Query: 65 DECSMASAAALILPGGNTWTEAIHNPILQLAERYIQKEILVAAICGATMGLAQAGLLNSR 124 DEC++ S LILPGG TW+E IH PIL+ + ++ +VAAICGAT LA G L++R Sbjct: 66 DECTLESKDLLILPGGTTWSEEIHQPILERIGQALKLGTIVAAICGATEALANMGYLDTR 125 Query: 125 PHTSNDLEYLRMVCPAYTGGDFYQMQSVVTDGTLITASGIAPLEFAVHVLKALDVMTPEK 184 HTSN+LEY +MVCP Y G FY++ S V+D L+TASGIAPLEFA+ VLK LDV P+ Sbjct: 126 KHTSNNLEYTKMVCPNYKGEKFYELGSAVSDANLVTASGIAPLEFAMEVLKKLDVFAPDT 185 Query: 185 LKAWYSLYKTQEAKYFYELMG 205 L AWY+L KT + +YF+ELM Sbjct: 186 LHAWYNLNKTHKPEYFFELMN 206 >Q2BBG5.1 Q2BBG5_9BACI Putative uncharacterized protein Length = 209 Score = 261 bits (666), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 123/203 (60%), Positives = 149/203 (73%) Query: 2 KHTVYLYVFDTMADWEIGYLTAELNSGRYYKKGLAPSTIVTVANEKTLVTTMGGLTIMPD 61 K YLYVFDTM+DWE GYL AELNSGRY+++G+AP ++TV +K V TMGG+TI PD Sbjct: 3 KRKAYLYVFDTMSDWEAGYLIAELNSGRYFRRGVAPIEVMTVGADKNPVKTMGGVTIQPD 62 Query: 62 ITVDECSMASAAALILPGGNTWTEAIHNPILQLAERYIQKEILVAAICGATMGLAQAGLL 121 ++++E S+ S LILPGG+TW E IH IL+ A +Q+ +VAAICGATMGLA GLL Sbjct: 63 LSMEEFSLGSQDILILPGGDTWLEPIHESILEEASNGLQRGTIVAAICGATMGLANKGLL 122 Query: 122 NSRPHTSNDLEYLRMVCPAYTGGDFYQMQSVVTDGTLITASGIAPLEFAVHVLKALDVMT 181 ++R HTSND YL+ VCP Y G YQ + V DG L+TASGIAPLEFA VLK LDV Sbjct: 123 DTRRHTSNDPGYLKAVCPLYAGESLYQFEPAVADGNLVTASGIAPLEFASEVLKMLDVCK 182 Query: 182 PEKLKAWYSLYKTQEAKYFYELM 204 PE L+AW+ L KT EA YFYELM Sbjct: 183 PETLQAWFKLNKTHEAGYFYELM 205 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,233,101,309 Number of extensions: 47077739 Number of successful extensions: 126611 Number of sequences better than 1.0e-05: 288 Number of HSP's gapped: 126711 Number of HSP's successfully gapped: 288 Length of query: 205 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 76 Effective length of database: 1,845,385,268 Effective search space: 140249280368 Effective search space used: 140249280368 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)