BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0183.0010.p (431 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2BBT3.1 Q2BBT3_9BACI Sugar ABC transporter sugar-binding protein 144 2e-32 A6TTD0.1 A6TTD0_ALKMQ Extracellular solute-binding protein, fami... 142 5e-32 A8VRA1.1 A8VRA1_9BACI ABC-type sugar transport system periplasmi... 139 5e-31 Q8ELU3.1 Q8ELU3_OCEIH Sugar ABC transporter sugar-binding protein 138 9e-31 B3KA21.1 B3KA21_9BACI Extracellular solute-binding protein family 1 136 4e-30 >Q2BBT3.1 Q2BBT3_9BACI Sugar ABC transporter sugar-binding protein Length = 433 Score = 144 bits (363), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 112/366 (30%), Positives = 177/366 (48%), Gaps = 16/366 (4%) Query: 71 VELKYEYIQDETYKTKLSVALAANELPDVFQQHAGKSYRTPVLQSKTVAPLNDTLESTGL 130 V++ E + +E YK KL V +N+LPDV A + P + K PL+D LE L Sbjct: 72 VKVDLEVLSNEQYKDKLKVLSTSNQLPDVGLTWAA-GFLDPYVSGKKFTPLDDLLEGD-L 129 Query: 131 GAQFLDNQL-VTEEDGNIYSVPSNISTTLVLYYNKKLISELGAAAPATWDDLQALIKTAN 189 +F+ E DG+ Y +P ++ V +YNK + + G AP T+++ Q +++T + Sbjct: 130 KDKFVSGTAEAYEVDGSTYGLPLELNIATV-FYNKAIFEKYGLEAPKTYEEFQNVVETLD 188 Query: 190 DKGMIPIALGGKERWQGDLLYDMLVAR-EDVDAFDKAINGQAKFTDTPFVEAANKVVTLV 248 G+ PIALG K+RW G L Y L R D KAI+ F D V AA KV LV Sbjct: 189 KNGVAPIALGNKDRWTGSLWYMYLADRIGGGDVLTKAIDRSGSFEDPALVSAAEKVQELV 248 Query: 249 NSNAFQKGFLGSAYLDAQELFKNDKAVMWIDGSFNFSSLST------AMGDNLGYIAFPK 302 + NAF KGF G A +A+ +F N++A M++ +++ + +T D++GY FP Sbjct: 249 DMNAFVKGFNGLADEEAKSMFMNEQAAMYLIATWDLPNYTTNEDVPQEFRDSVGYFNFPT 308 Query: 303 TGAEDVLSATIGFQNAQAPYSLFVNNSSANLDKAKEFAIRLSLKLNDEFVRK-GLPGYAK 361 + + + +G LFV S ++AKEFA + ++ V G+ K Sbjct: 309 VDGKGDMDSFVGGPGV----GLFVAEDSDVKEEAKEFASYFIQQWGEKSVTDAGVIPATK 364 Query: 362 SEVKSESQNKELSAYAADINKTSKTQAMWFGLLSADVGQEYRDLTQALYGGQLTADEFTA 421 + S D+N S +SAD Q + D+ Q+L+G +++ ++F Sbjct: 365 VDAASLDLPDMYVDVLEDLNSASNITLFADVQMSADTAQVHLDMIQSLFGKEVSPEDFAK 424 Query: 422 QLETLL 427 E L Sbjct: 425 AHEEAL 430 >A6TTD0.1 A6TTD0_ALKMQ Extracellular solute-binding protein, family 1 Length = 441 Score = 142 bits (359), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 115/371 (30%), Positives = 184/371 (49%), Gaps = 25/371 (6%) Query: 71 VELKYEYIQDETYKTKLSVALAANELPDV-FQQHAGKSYRTPVLQSKTVAPLNDTLESTG 129 V++ E +++E YKTKL V A+NELPDV AG + P ++ PL+D L+ G Sbjct: 77 VKIVQEVLENEQYKTKLQVISASNELPDVGMTWPAG--FMEPYVKGNRFTPLDDLLKG-G 133 Query: 130 LGAQFLDNQLVTEE---DGNIYSVPSNISTTLVLYYNKKLISELGAAAPATWDDLQALIK 186 L F+ TE DG Y+ P ++ + LYYNK + +E P T++D + ++K Sbjct: 134 LKESFVAG--TTEAYAVDGKTYAFPLELNI-VPLYYNKSIFAEYNLEVPETYEDFKHVVK 190 Query: 187 TANDKGMIPIALGGKERWQGDLLYDMLVAR-EDVDAFDKAINGQAKFTDTPFVEAANKVV 245 T D ++PIALG K+RW G L Y L R + AIN F D V+AA +V Sbjct: 191 TLVDNNVVPIALGNKDRWTGSLWYMYLADRLGGPEILTNAINRSGTFEDESLVKAAEEVQ 250 Query: 246 TLVNSNAFQKGFLGSAYLDAQELFKNDKAVMWIDGSFNFSSLST------AMGDNLGYIA 299 TLV+ NAF +GF G + + + F N+KA M++ GS+ + +T DN+GY Sbjct: 251 TLVDMNAFVRGFNGLSNDEGKSEFLNEKAAMYLMGSWELPNFTTNEEIPQEFRDNVGYFK 310 Query: 300 FPKT-GAEDVLSATIGFQNAQAPYSLFVNNSSANLDKAKEFAIRLSLKLNDE-FVRKGL- 356 FP G + + + +G LFV +S+ +++K+F ++ VR G+ Sbjct: 311 FPTVEGGKGDIDSWVGGPGV----GLFVAENSSVKEESKKFVKFFVESWGEQAVVRAGVT 366 Query: 357 PGYAKSEVKSESQNKELSAYAADINKTSKTQAMWFGLLSADVGQEYRDLTQALYGGQLTA 416 P A E + D+ + S +SA V + + ++ Q+L+G Q+T Sbjct: 367 PATAVDTTALELPQLYIDVL-NDLGRASNMTLFADVQMSAAVAETHLNMIQSLFGNQVTP 425 Query: 417 DEFTAQLETLL 427 +++ + E L Sbjct: 426 EDYAKRHEEAL 436 >A8VRA1.1 A8VRA1_9BACI ABC-type sugar transport system periplasmic component-like protein Length = 454 Score = 139 bits (350), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 112/371 (30%), Positives = 184/371 (49%), Gaps = 24/371 (6%) Query: 71 VELKYEYIQDETYKTKLSVALAANELPDV-FQQHAGKSYRTPVLQSKTVAPLNDTLESTG 129 V ++ E +++E YK K+ V ++N+LPDV AG Y P + APL+D L+ G Sbjct: 90 VTVELEVLENEQYKNKMQVISSSNQLPDVGMTWPAG--YMEPYVNGNMFAPLDDILDEDG 147 Query: 130 LGAQFLDNQL-VTEEDGNIYSVPSNISTTLVLYYNKKLISELGAAAPATWDDLQALIKTA 188 L F+ L E +G+ Y++P ++ V +YNK + E G P T+DD I T Sbjct: 148 LRDDFVAGTLEAYEMNGDSYALPLELNIAPV-FYNKAIFDEYGVDVPETFDDFMDAIDTF 206 Query: 189 NDKGMIPIALGGKERWQGDLLYDMLVAR-EDVDAFDKAINGQAKFTDTPFVEAANKVVTL 247 + M PIALG ++RW G L Y L R DA + AI+ F D +EAA + V + Sbjct: 207 VENDMAPIALGNRDRWTGSLWYMYLADRIGGPDALNAAIDRSGSFEDERLIEAAERTVDM 266 Query: 248 VNSNAFQKGFLGSAYLDAQELFKNDKAVMWIDGSFNFSSLST------AMGDNLGYIAFP 301 V S+AF +GF G + +A+ F N A M++ GS++ + +T DN+G+ FP Sbjct: 267 VQSDAFIRGFNGLSDQEAKSEFMNSNAAMYLIGSWDLPNYTTNEEVPQEFRDNIGFFKFP 326 Query: 302 KT-GAEDVLSATIGFQNAQAPYSLFVNNSSANLDKAKEFAIRLSLKLNDEFVRKG--LPG 358 + G + +++ +G LFV +S ++AK F + + V +PG Sbjct: 327 EVDGGDGDINSWVGGPGV----GLFVAENSDIQEEAKAFVKYFAEEWGKYSVEDAGVIPG 382 Query: 359 YAKSEVKSESQN-KELSAYAAD-INKTSKTQAMWFGLLSADVGQEYRDLTQALYGGQLTA 416 ++V +++ + +L D +N S +SA V + + + QAL+G +T Sbjct: 383 ---TQVDTDAVDLPDLFVEVLDELNNASNITLFADVQMSAGVAETHLNQIQALFGEAVTP 439 Query: 417 DEFTAQLETLL 427 ++F + E L Sbjct: 440 EDFAREHEEAL 450 >Q8ELU3.1 Q8ELU3_OCEIH Sugar ABC transporter sugar-binding protein Length = 435 Score = 138 bits (348), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 118/430 (27%), Positives = 191/430 (44%), Gaps = 34/430 (7%) Query: 21 TSCGGAEANNGGTQGQEAAAADSK--KSVTLWIFDADPMY-----QSXXXXXXXXVGVEL 73 +C G E N +A+ DSK K + LW + + Q G+++ Sbjct: 21 VACSGEEDEN-------SASGDSKTVKFMHLWPEGSSAQHYKVVNQIIEDFETENEGIDI 73 Query: 74 KYEYIQDETYKTKLSVALAANELPDVFQQHAGKSYRTPVLQSKTVAPLNDTLESTGLGAQ 133 + E + +E YK K+ V +NELPDV A Y P + A L+D LE L + Sbjct: 74 ELEVLSNEQYKEKIKVQSTSNELPDVGMTWAA-GYMEPFVDGNMFASLDDILEEDNLSEE 132 Query: 134 FLDNQL-VTEEDGNIYSVPS--NISTTLVLYYNKKLISELGAAAPATWDDLQALIKTAND 190 F+ D Y +P NIS+ +YYNK + E G P T D+ ++ T N+ Sbjct: 133 FVAGTTDAFSIDDTAYGLPLELNISS---IYYNKAIFDEYGLEEPTTLDEFNQVVDTLNE 189 Query: 191 KGMIPIALGGKERWQGDLLYDMLVAR--EDVDAFDKAINGQAKFTDTPFVEAANKVVTLV 248 +IP+ALG ++ W G + Y L R D D AI+ F D ++AA + LV Sbjct: 190 NDVIPVALGNRDAWTGSMWYMYLANRLGGDEDVLTSAIDRSGSFEDPALLKAAEHLQGLV 249 Query: 249 NSNAFQKGFLGSAYLDAQELFKNDKAVMWIDGSFNFSSLST------AMGDNLGYIAFPK 302 N N+F GF G A +A+ +F +A M++ +++ + +T D++GY FP+ Sbjct: 250 NDNSFVSGFNGLADQEAKSMFMESQAAMYLIATWDLPNYTTNEDVPQEFRDSVGYFPFPQ 309 Query: 303 TGAEDVLSATIGFQNAQAPYSLFVNNSSANLDKAKEFAIRLSLKLNDEFVRK-GLPGYAK 361 ++ +G LFV+ +S ++AK F + + V + G+ K Sbjct: 310 VDGSGDFNSYVGGPGV----GLFVSENSEVKEEAKTFVSYFVKEWGERAVTEVGIIPATK 365 Query: 362 SEVKSESQNKELSAYAADINKTSKTQAMWFGLLSADVGQEYRDLTQALYGGQLTADEFTA 421 + ++ + D+N S +SA Q + D+ QAL+GG++T +EF Sbjct: 366 IDGEALDLPELYVEVLDDLNNASNITLYADVQMSASAAQVHLDMIQALFGGEITPEEFVK 425 Query: 422 QLETLLRTAE 431 E L E Sbjct: 426 THEETLANEE 435 >B3KA21.1 B3KA21_9BACI Extracellular solute-binding protein family 1 Length = 441 Score = 136 bits (343), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 109/369 (29%), Positives = 182/369 (49%), Gaps = 31/369 (8%) Query: 71 VELKYEYIQDETYKTKLSVALAANELPDVFQQHAGKSYRTPVLQSKTVAPLNDTLESTGL 130 V +K E + +E YK KL V A+NELPDV A + P ++ AP++D L+ L Sbjct: 78 VTIKQEVLDNEQYKNKLKVLSASNELPDVGVTWAA-GFLKPYVEGNLFAPVDDVLDGQ-L 135 Query: 131 GAQFLDNQLVTEE---DGNIYSVPSNISTTLVLYYNKKLISELGAAAPATWDDLQALIKT 187 G +F+ TE DG Y++P + V YYNK + + P T+++ + ++KT Sbjct: 136 GDKFVAG--TTEAYAIDGKTYALPLEFNIAPV-YYNKAIFEQHNLEIPQTYEEFKTVVKT 192 Query: 188 ANDKGMIPIALGGKERWQGDLLYDMLVAR-EDVDAFDKAINGQAKFTDTPFVEAANKVVT 246 +D G+ PIALG K+RW G L Y L R + AI+G F D V+AA +V + Sbjct: 193 LSDNGIAPIALGNKDRWTGSLWYMYLADRIGGQEKLAGAISGSGSFKDESLVKAAAEVQS 252 Query: 247 LVNSNAFQKGFLGSAYLDAQELFKNDKAVMWIDGSFNFSSLST------AMGDNLGYIAF 300 LV+ NAF KGF G + +A+ F N KA M++ G++ + +T D++G+ F Sbjct: 253 LVDGNAFVKGFNGLSNEEAKSEFLNGKAAMYMMGTWELPNFTTNEEIPQEFRDSVGFFKF 312 Query: 301 PKT-GAEDVLSATIGFQNAQAPYSLFVNNSSANLDKAKEFAIRLSLKLNDEFVRKG--LP 357 P G + +++ +G LF+ +S +++K+F K ++ V +P Sbjct: 313 PTVEGGKGDINSWVGGPGV----GLFIGENSKVKEESKKFVEYFVTKWGEQSVTGAGVIP 368 Query: 358 GYAKSEVKSESQNKELSAYAADI----NKTSKTQAMWFGLLSADVGQEYRDLTQALYGGQ 413 K ++ EL D+ NK S + A+ + + ++ QAL+G Sbjct: 369 A-----TKVDTSTLELPQLYIDLFDEMNKASSITLFADVQMQANAAETHLNMIQALFGKA 423 Query: 414 LTADEFTAQ 422 +T ++F+ + Sbjct: 424 VTPEKFSEE 432 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.129 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,310,605,896 Number of extensions: 90477633 Number of successful extensions: 177552 Number of sequences better than 1.0e-05: 542 Number of HSP's gapped: 177517 Number of HSP's successfully gapped: 545 Length of query: 431 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 292 Effective length of database: 1,751,175,118 Effective search space: 511343134456 Effective search space used: 511343134456 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)