BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0183.0001.p (1288 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0TD58.1 B0TD58_HELMI Multidomain protein with s-layer homology ... 381 e-103 A3DF10.1 A3DF10_CLOTH Cellulosome anchoring protein, cohesin region 321 3e-85 P71143.1 P71143_CLOTM Scaffolding dockerin binding protein A 321 3e-85 B4BET0.1 B4BET0_CLOTM Cellulosome anchoring protein cohesin region 321 3e-85 Q53I45.1 Q53I45_9BACL Xylanase XynA GH 10 (Fragment) 282 2e-73 >B0TD58.1 B0TD58_HELMI Multidomain protein with s-layer homology region, glug motif, ig motif, i-set domain, pkd domain Length = 2940 Score = 381 bits (978), Expect = e-103, Method: Compositional matrix adjust. Identities = 199/368 (54%), Positives = 255/368 (69%), Gaps = 1/368 (0%) Query: 922 NNGTAFVKVLDLAKAKEFMEKNVTLDIPAAQGVNSYSVGLPAAALTSGTKDDKLTISTEF 981 N G A + LAK L +P+ GVNSY+VG+PAA+L+ + LT ST Sbjct: 2572 NAGNAATDLGTLAKDIFTGAGTAVLTVPSISGVNSYTVGIPAASLSGSQGEGALTFSTGA 2631 Query: 982 GQVVISGNMLTGTTESSGKEVTMEIGKGDKAKLPAEVKAALGDRPVIQLSLKVDGKETAW 1041 G + I ML G E+ GK+ + I +GD+ LP +VK A+GDRP++QL+L +DGK+T W Sbjct: 2632 GSITIPSGMLAGIPETEGKKAGITIARGDRTSLPEDVKTAIGDRPIVQLTLTLDGKQTNW 2691 Query: 1042 SNPFAPVTVSVPYKPSADELKNPEMIVVWYIDGSGDVLTVPSGRYAPKTGMVTFTTTHFS 1101 SNP APVTVS+PY P+A EL NPE IVVWYIDGSG+V++VP+GRY P TG VTFTTTHFS Sbjct: 2692 SNPDAPVTVSIPYTPTAAELANPEHIVVWYIDGSGNVVSVPNGRYDPATGTVTFTTTHFS 2751 Query: 1102 SYAVAYVSRTFTDLGTAVWAKNAVEVLASKDILKTEGH-VFNPSTGITRADFLYSLVRAL 1160 YAVAYV +TF DLG A WA+ VEV+ASK I+ G F+P+ ITRAD+L L+R L Sbjct: 2752 YYAVAYVHKTFGDLGGAEWARKPVEVMASKGIISGTGKGTFSPAANITRADYLVLLIRTL 2811 Query: 1161 GLNARVNGNFSDVQKSAYYYNEIAIAKALGITNGIDNDRFGSAYKITRQDMMVLTERALK 1220 GL A +GNF DV+ AYYY + IAK LGI G N+RF I+RQDMMVLT RAL+ Sbjct: 2812 GLMAEFDGNFDDVEPGAYYYEALGIAKKLGIAAGSGNNRFNPKESISRQDMMVLTARALE 2871 Query: 1221 LEKKLSNQAEAADLERFTDKSEVASYAVNSVAAMVKEGLIEGSGNKVNPAGNTTKAEAAV 1280 + L L++++DK ++A YA NS+A +VKEGLI+GSG+++NP N T+AEAAV Sbjct: 2872 KFRGLKAVDANGLLDKYSDKGDIAGYAANSLATLVKEGLIKGSGDRLNPRANATRAEAAV 2931 Query: 1281 FLYRLYNK 1288 FLYR+YNK Sbjct: 2932 FLYRIYNK 2939 >A3DF10.1 A3DF10_CLOTH Cellulosome anchoring protein, cohesin region Length = 631 Score = 321 bits (822), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 178/390 (45%), Positives = 240/390 (61%), Gaps = 16/390 (4%) Query: 914 KVTMQATVNNGTA----------FVKVLDLAKAKEFMEKNVTLDIPAAQGVNSYSVGLPA 963 KV ++ ++N T K LD AK E +K V L+I + ++Y LPA Sbjct: 243 KVELEPVLDNATGEAKAAIDEEKLNKALDEAKKSE-DDKLVELNIKKVENADAYIQQLPA 301 Query: 964 AALTSGTKDDKLTISTEFGQVVISGNMLTGTTES----SGKEVTMEIGKGDKAKLPAEVK 1019 L + KL I+TE G + + NML S + V I K +L AE+K Sbjct: 302 KFLIKSDAEYKLRIATEQGIIEVPANMLNTADISKLVKNDSVVEFVIRKVKVDELGAELK 361 Query: 1020 AALGDRPVIQLSLKVDGKETAWSNPFAPVTVSVPYKPSADELKNPEMIVVWYIDGSGDVL 1079 +G+RPVI +S+ VDGK+ WSN A V +S+PYKP A EL+N E IVV +ID +G + Sbjct: 362 EKIGNRPVIDISVVVDGKKVEWSNYKAKVKISIPYKPDAKELENHEHIVVLHIDDAGKAV 421 Query: 1080 TVPSGRYAPKTGMVTFTTTHFSSYAVAYVSRTFTDLGTAVWAKNAVEVLASKDILK-TEG 1138 +VPSG+Y P G+VTF T H S YAV+YV +TF D+G+ WAK +EVLASK ++ T Sbjct: 422 SVPSGKYEPSLGVVTFETNHLSKYAVSYVYKTFADIGSYAWAKKQIEVLASKGVINGTSD 481 Query: 1139 HVFNPSTGITRADFLYSLVRALGLNARVNGNFSDVQKSAYYYNEIAIAKALGITNGIDND 1198 F P ITRADF+ LV+ALGL A V NF DV + YYY + IAK LGIT G+ N+ Sbjct: 482 TTFTPQADITRADFMILLVKALGLTAEVTSNFDDVSEKDYYYEYVGIAKELGITTGVGNN 541 Query: 1199 RFGSAYKITRQDMMVLTERALKLEKKLSNQAEAADLERFTDKSEVASYAVNSVAAMVKEG 1258 +F KITRQDMMVLT AL++ K+S+ AD+ERF+DK ++ASYAV VA +VKEG Sbjct: 542 KFNPKAKITRQDMMVLTTNALRIAGKISSTGTRADVERFSDKDQIASYAVEGVATLVKEG 601 Query: 1259 LIEGSGNKVNPAGNTTKAEAAVFLYRLYNK 1288 ++ GSG+ +NP GN ++AE A +Y++Y K Sbjct: 602 IVVGSGDIINPRGNASRAELAAIIYKIYYK 631 >P71143.1 P71143_CLOTM Scaffolding dockerin binding protein A Length = 631 Score = 321 bits (822), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 178/390 (45%), Positives = 240/390 (61%), Gaps = 16/390 (4%) Query: 914 KVTMQATVNNGTA----------FVKVLDLAKAKEFMEKNVTLDIPAAQGVNSYSVGLPA 963 KV ++ ++N T K LD AK E +K V L+I + ++Y LPA Sbjct: 243 KVELEPVLDNATGEAKAAIDEEKLNKALDEAKKSE-DDKLVELNIKKVENADAYIQQLPA 301 Query: 964 AALTSGTKDDKLTISTEFGQVVISGNMLTGTTES----SGKEVTMEIGKGDKAKLPAEVK 1019 L + KL I+TE G + + NML S + V I K +L AE+K Sbjct: 302 KFLIKSDAEYKLRIATEQGIIEVPANMLNTADISKLVKNDSVVEFVIRKVKVDELGAELK 361 Query: 1020 AALGDRPVIQLSLKVDGKETAWSNPFAPVTVSVPYKPSADELKNPEMIVVWYIDGSGDVL 1079 +G+RPVI +S+ VDGK+ WSN A V +S+PYKP A EL+N E IVV +ID +G + Sbjct: 362 EKIGNRPVIDISVVVDGKKVEWSNYKAKVKISIPYKPDAKELENHEHIVVLHIDDAGKAV 421 Query: 1080 TVPSGRYAPKTGMVTFTTTHFSSYAVAYVSRTFTDLGTAVWAKNAVEVLASKDILK-TEG 1138 +VPSG+Y P G+VTF T H S YAV+YV +TF D+G+ WAK +EVLASK ++ T Sbjct: 422 SVPSGKYEPSLGVVTFETNHLSKYAVSYVYKTFADIGSYAWAKKQIEVLASKGVINGTSD 481 Query: 1139 HVFNPSTGITRADFLYSLVRALGLNARVNGNFSDVQKSAYYYNEIAIAKALGITNGIDND 1198 F P ITRADF+ LV+ALGL A V NF DV + YYY + IAK LGIT G+ N+ Sbjct: 482 TTFTPQADITRADFMILLVKALGLTAEVTSNFDDVSEKDYYYEYVGIAKELGITTGVGNN 541 Query: 1199 RFGSAYKITRQDMMVLTERALKLEKKLSNQAEAADLERFTDKSEVASYAVNSVAAMVKEG 1258 +F KITRQDMMVLT AL++ K+S+ AD+ERF+DK ++ASYAV VA +VKEG Sbjct: 542 KFNPKAKITRQDMMVLTTNALRIAGKISSTGTRADVERFSDKDQIASYAVEGVATLVKEG 601 Query: 1259 LIEGSGNKVNPAGNTTKAEAAVFLYRLYNK 1288 ++ GSG+ +NP GN ++AE A +Y++Y K Sbjct: 602 IVVGSGDIINPRGNASRAELAAIIYKIYYK 631 >B4BET0.1 B4BET0_CLOTM Cellulosome anchoring protein cohesin region Length = 631 Score = 321 bits (822), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 178/390 (45%), Positives = 240/390 (61%), Gaps = 16/390 (4%) Query: 914 KVTMQATVNNGTA----------FVKVLDLAKAKEFMEKNVTLDIPAAQGVNSYSVGLPA 963 KV ++ ++N T K LD AK E +K V L+I + ++Y LPA Sbjct: 243 KVELEPVLDNATGEAKAAIDEEKLNKALDEAKKSE-DDKLVELNIKKVENADAYIQQLPA 301 Query: 964 AALTSGTKDDKLTISTEFGQVVISGNMLTGTTES----SGKEVTMEIGKGDKAKLPAEVK 1019 L + KL I+TE G + + NML S + V I K +L AE+K Sbjct: 302 KFLIKSDAEYKLRIATEQGIIEVPANMLNTADISKLVKNDSVVEFVIRKVKVDELGAELK 361 Query: 1020 AALGDRPVIQLSLKVDGKETAWSNPFAPVTVSVPYKPSADELKNPEMIVVWYIDGSGDVL 1079 +G+RPVI +S+ VDGK+ WSN A V +S+PYKP A EL+N E IVV +ID +G + Sbjct: 362 EKIGNRPVIDISVVVDGKKVEWSNYKAKVKISIPYKPDAKELENHEHIVVLHIDDAGKAV 421 Query: 1080 TVPSGRYAPKTGMVTFTTTHFSSYAVAYVSRTFTDLGTAVWAKNAVEVLASKDILK-TEG 1138 +VPSG+Y P G+VTF T H S YAV+YV +TF D+G+ WAK +EVLASK ++ T Sbjct: 422 SVPSGKYEPSLGVVTFETNHLSKYAVSYVYKTFADIGSYAWAKKQIEVLASKGVINGTSD 481 Query: 1139 HVFNPSTGITRADFLYSLVRALGLNARVNGNFSDVQKSAYYYNEIAIAKALGITNGIDND 1198 F P ITRADF+ LV+ALGL A V NF DV + YYY + IAK LGIT G+ N+ Sbjct: 482 TTFTPQADITRADFMILLVKALGLTAEVTSNFDDVSEKDYYYEYVGIAKELGITTGVGNN 541 Query: 1199 RFGSAYKITRQDMMVLTERALKLEKKLSNQAEAADLERFTDKSEVASYAVNSVAAMVKEG 1258 +F KITRQDMMVLT AL++ K+S+ AD+ERF+DK ++ASYAV VA +VKEG Sbjct: 542 KFNPKAKITRQDMMVLTTNALRIAGKISSTGTRADVERFSDKDQIASYAVEGVATLVKEG 601 Query: 1259 LIEGSGNKVNPAGNTTKAEAAVFLYRLYNK 1288 ++ GSG+ +NP GN ++AE A +Y++Y K Sbjct: 602 IVVGSGDIINPRGNASRAELAAIIYKIYYK 631 >Q53I45.1 Q53I45_9BACL Xylanase XynA GH 10 (Fragment) Length = 1467 Score = 282 bits (721), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 163/369 (44%), Positives = 223/369 (60%), Gaps = 12/369 (3%) Query: 924 GTAFVKVLDLAKAKEFMEKNVTLDIPAAQGVNSYSVGLPAAALTSGTKDD-KLTISTEFG 982 G K LD A +K V +D+P +Y+V LP +L S +D +LT Sbjct: 1102 GDDLKKALDQAAPAAGGKKQVIIDVPLQANAATYAVQLPTQSLKS--QDGYQLTAKIANA 1159 Query: 983 QVVISGNMLTGTTESSGKEVTMEIGKGDKAKLPAEVKAALGDRPVIQLSLKVDGKETAWS 1042 + I NML T ++ +V++ + K + A + +G+RPVI LSL G AW+ Sbjct: 1160 FIQIPSNMLANTNVTT-DQVSIRVAKASLDNVDAATRELIGNRPVIDLSLVAGGNVIAWN 1218 Query: 1043 NPFAPVTVSVPYKPSADELKNPEMIVVWYIDGSGDVLTVPSGRYAPKTGMVTFTTTHFSS 1102 NP APVTV+VPY P+A+ELK+PE I++WYIDGSG VP+ RY G V F TTHFS+ Sbjct: 1219 NPTAPVTVAVPYAPTAEELKHPEHILIWYIDGSGKATPVPNSRYDAALGAVVFQTTHFST 1278 Query: 1103 YAVAYVSRTFTDLGTAVWAKNAVEVLASKDILKTEG-HVFNPSTGITRADFLYSLVRALG 1161 YA V TF DL WAK A++ +AS+ ++K G + F+P+ I RADF+ LVRAL Sbjct: 1279 YAAVSVFTTFGDLAKVPWAKEAIDAMASRGVIKGTGENTFSPAASIKRADFIALLVRALE 1338 Query: 1162 LNARVNGN---FSDVQKSAYYYNEIAIAKALGITNGIDNDRFGSAYKITRQDMMVLTERA 1218 L+ + FSDV +AYYYNE+A+AK LGI G +++ F I+RQDMMVLT RA Sbjct: 1339 LHGTGTTDTAMFSDVPANAYYYNELAVAKQLGIATGFEDNTFKPDSSISRQDMMVLTTRA 1398 Query: 1219 LKLEKKLSNQAEA-ADLERFTDKSEVASYAVNSVAAMVKEGLIEGSGNKVNPAGNTTKAE 1277 L + L Q A L F+D + VA YA +SVAA+VK G+++GSG+K+ P T+AE Sbjct: 1399 LAV---LGKQLPAGGSLNAFSDAASVAGYAQDSVAALVKAGVVQGSGSKLAPNDQLTRAE 1455 Query: 1278 AAVFLYRLY 1286 AAV LYR++ Sbjct: 1456 AAVILYRIW 1464 Score = 96.3 bits (238), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 115/475 (24%), Positives = 182/475 (38%), Gaps = 144/475 (30%) Query: 277 QSAVAPLKSVYNKENGLFMVG-AIGTGPVTGTRAN----HYEIFVDGNNLKADGTHPRGP 331 Q +APLK VY + F++G AI + GTR H+++ GN +K D P Sbjct: 519 QKDLAPLKDVYKND---FLIGNAISAEDLEGTRLELLKMHHDVVTAGNAMKPDALQP--- 572 Query: 332 KWLKSVTGEALNGATTAPGLAEYSFPTNAYQAIRDSGTPGQYKSHGHVLAWYNQAPGWMT 391 + +F A A+ D K HGHVL W+ Q+P W+ Sbjct: 573 --------------------TKGNFTFTAADAMIDKVLAEGMKMHGHVLVWHQQSPAWLN 612 Query: 392 QIIPANLASGYNGGADFYGLGNGVTTTVKVDKEMARRVQFNHTMYVMRHFLTTDTKYGSS 451 N TV + ++ A H VM+HF Sbjct: 613 TKKDDN------------------NNTVPLGRDEALDNLRTHIQTVMKHF---------- 644 Query: 452 ESRGVIPFNSWDVLNEEVHESRHSELIPGDANSWRTSLKHTNWLAAMSDDQIGGDITDHY 511 VI SWDV+NE ++++ P + ++ SL+ T W A+ D + Sbjct: 645 -GNKVI---SWDVVNEAMNDN------PSNPADYKASLRQTPWYQAIGSDYV-------- 686 Query: 512 VYLLFKNAHIAAPNAKMAEAYKANYANLPEYMKLDGHDNEGSIDAYIVDNPP----KLTY 567 + A +AA ++D P KL Y Sbjct: 687 -----EQAFLAARE--------------------------------VLDENPSWNIKLYY 709 Query: 568 NDYGLATRSKARTVYNMVLELNTAWRYDPLYDGRPLIEDIGIQGHDAVGKTLASDNQYAM 627 NDY ++KA +YNMV ++N RY ++G+ LI+ +G+QGH + T + + ++ Sbjct: 710 NDYNEDNQNKATAIYNMVKDIND--RYAAAHNGKLLIDGVGMQGHYNI-NTNPDNVKLSL 766 Query: 628 ALYASLVDRGLLSGITYSELDLKVXXXXXXXXXXXXXVLNVRQSDALGYQYALLYKMFNK 687 + SL G+ ++ SELD+ L V Q+ Y YA L+K++ + Sbjct: 767 EKFISL---GV--EVSVSELDVTAGNNYTLPEN-----LAVGQA----YLYAQLFKLYKE 812 Query: 688 FAPYIDHIIXXXXXXXXXXXXY------VLFDGQSNANAGYYGAMKPDRFILGHS 736 A DHI + +LFD A YYG + PD+++ H+ Sbjct: 813 HA---DHIARVTFWGMDDNTSWRAENNPLLFDKNLQAKPAYYGVIDPDKYMEEHA 864 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.131 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 7,560,324,945 Number of extensions: 328647628 Number of successful extensions: 625121 Number of sequences better than 1.0e-05: 512 Number of HSP's gapped: 625107 Number of HSP's successfully gapped: 539 Length of query: 1288 Length of database: 3,060,696,203 Length adjustment: 148 Effective length of query: 1140 Effective length of database: 1,666,385,983 Effective search space: 1899680020620 Effective search space used: 1899680020620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 138 (57.8 bits)