BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0181.0004.p (316 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DAG6.1 B1DAG6_9BACL Binding-protein-dependent transport system... 466 e-129 B3K542.1 B3K542_9BACI Binding-protein-dependent transport system... 383 e-104 A4IP82.1 A4IP82_GEOTN Putative uncharacterized protein 367 e-100 C3J6V5.1 C3J6V5_9BACI Binding-protein-dependent transport system... 364 7e-99 B7YFA2.1 B7YFA2_9BACI Binding-protein-dependent transport system... 364 7e-99 >B1DAG6.1 B1DAG6_9BACL Binding-protein-dependent transport systems inner membrane component Length = 315 Score = 466 bits (1199), Expect = e-129, Method: Compositional matrix adjust. Identities = 219/313 (69%), Positives = 258/313 (82%) Query: 4 NTIQQVNPGKPLRQSRKKKVKQPWMLHLMVLPAAVMVFIFSYIPMGGILMAFQDYKPKPG 63 NT + P + ++++ KQPW+LH MVLPA ++ F+FSYIPM G++MAFQDYK + G Sbjct: 3 NTTNSLELSTPPKAGKRRRYKQPWILHFMVLPAVLLSFVFSYIPMSGVVMAFQDYKARLG 62 Query: 64 LFGSPWVGLKHFEYMWQNDYFLQITWNTLFFAGTKIIMNLIIPFFFALLLNEVRKMALKR 123 SPWVG KHF YM+ NDYF++I NTL FA +KI++NLIIPF FALLLNEVR M LKR Sbjct: 63 FLHSPWVGFKHFNYMFSNDYFVKIIGNTLLFASSKIVLNLIIPFAFALLLNEVRSMGLKR 122 Query: 124 TIQTLVYLPHFLSWVTLSGILIDMLAQTGLINQFISSVFGIKPIFFLGDGSWFRFTIIFS 183 ++QTLVYLPHFLSWVTLSGILID+L QTG++NQF++S FGI+PIFFLGDG+WFR TI+ S Sbjct: 123 SVQTLVYLPHFLSWVTLSGILIDVLGQTGIVNQFLTSAFGIQPIFFLGDGTWFRMTIVVS 182 Query: 184 DVWKEFGFNMIIFLAALSGISPSLYEAAEVDGAGRWKQTIYXXXXXXXXXXXXXXXXXLG 243 DVWKEFGFN I+FLAALSGI+P+LYEAAEVDGA RWKQT++ +G Sbjct: 183 DVWKEFGFNTIVFLAALSGINPALYEAAEVDGATRWKQTMHITLPSLLPMLIVVATLAMG 242 Query: 244 NVLNANFDQVFNLYSPLIYQQGDIIDTFVYREGLLSGQFSFATAVNLFKSAISLILIVIS 303 NVLNANFDQVFNLYSP+IYQQGDIIDTFVYREGLLSGQFSFATAV LFKS ISL+LIVIS Sbjct: 243 NVLNANFDQVFNLYSPMIYQQGDIIDTFVYREGLLSGQFSFATAVGLFKSVISLVLIVIS 302 Query: 304 YRLAYRFAGYRIF 316 YR+AY+FAGYRIF Sbjct: 303 YRIAYKFAGYRIF 315 >B3K542.1 B3K542_9BACI Binding-protein-dependent transport systems inner membrane component Length = 296 Score = 383 bits (983), Expect = e-104, Method: Compositional matrix adjust. Identities = 178/296 (60%), Positives = 235/296 (79%), Gaps = 1/296 (0%) Query: 21 KKVKQPWMLHLMVLPAAVMVFIFSYIPMGGILMAFQDYKPKPGLFGSPWVGLKHFEYMWQ 80 K++K+ W H+M+LPA + +F+FSY+PMGGI+MAFQD+KP G+ GS WVGL +F Y+++ Sbjct: 2 KRMKKTWPFHVMLLPALICLFLFSYLPMGGIIMAFQDFKPWEGVGGSAWVGLDNFRYLFE 61 Query: 81 NDYFLQITWNTLFFAGTKIIMNLIIPFFFALLLNEVRKMALKRTIQTLVYLPHFLSWVTL 140 +Q+ WNT A KI+ NL+IPF FA+LLNE+RK AL+R+IQT+VYLPHFLSWV L Sbjct: 62 RQDSMQVIWNTFVIAVMKIVFNLLIPFVFAILLNEIRKTALQRSIQTMVYLPHFLSWVIL 121 Query: 141 SGILIDMLAQTGLINQFISSVFGIKPIFFLGDGSWFRFTIIFSDVWKEFGFNMIIFLAAL 200 GILI+MLA G +N+ +++V GI PIFFLGD +WFRFT+I SDVWKEFG+N I+FLAAL Sbjct: 122 GGILIEMLAVDGFVNRILAAV-GIDPIFFLGDNNWFRFTVIVSDVWKEFGYNTIVFLAAL 180 Query: 201 SGISPSLYEAAEVDGAGRWKQTIYXXXXXXXXXXXXXXXXXLGNVLNANFDQVFNLYSPL 260 +G+SP+LYEAAE+DGAGR KQT++ LGN+LNA FDQ+FNLY+PL Sbjct: 181 AGVSPTLYEAAEIDGAGRLKQTLHITIPSMIPITIVVGTLALGNILNAGFDQIFNLYNPL 240 Query: 261 IYQQGDIIDTFVYREGLLSGQFSFATAVNLFKSAISLILIVISYRLAYRFAGYRIF 316 +Y++GDIIDTFVYR +L+G+ F TA+ LFKS IS++LI++SYRLAY++AGYRIF Sbjct: 241 VYEKGDIIDTFVYRTAILNGEMGFGTAIGLFKSVISMVLILVSYRLAYKWAGYRIF 296 >A4IP82.1 A4IP82_GEOTN Putative uncharacterized protein Length = 316 Score = 367 bits (942), Expect = e-100, Method: Compositional matrix adjust. Identities = 179/301 (59%), Positives = 227/301 (75%), Gaps = 2/301 (0%) Query: 18 SRKK-KVKQPWMLHLMVLPAAVMVFIFSYIPMGGILMAFQDYKPKPGLFGSPWVGLKHFE 76 +RK+ K+K W LH M+LPA V+VF+F YIPM G++MAFQDY+P G SPWVGL+HF+ Sbjct: 16 TRKQWKLKGTWQLHTMLLPALVIVFVFQYIPMLGLVMAFQDYEPWLGFLHSPWVGLEHFK 75 Query: 77 YMWQNDYFLQITWNTLFFAGTKIIMNLIIPFFFALLLNEVRKMALKRTIQTLVYLPHFLS 136 M++ + Q+ WNTL + KII NL IP FALLLNE+ M KRT+QT+VYLPHFLS Sbjct: 76 TMFEYEDARQVIWNTLVISTLKIIFNLAIPLAFALLLNEIYVMKFKRTVQTIVYLPHFLS 135 Query: 137 WVTLSGILIDMLA-QTGLINQFISSVFGIKPIFFLGDGSWFRFTIIFSDVWKEFGFNMII 195 WV L GILIDML+ + G++N+ ++++FGI+PIFFLGD WFR ++ +DVWKE GFN I+ Sbjct: 136 WVILGGILIDMLSPEGGMVNRVLTALFGIEPIFFLGDNDWFRTVVVVTDVWKECGFNTIV 195 Query: 196 FLAALSGISPSLYEAAEVDGAGRWKQTIYXXXXXXXXXXXXXXXXXLGNVLNANFDQVFN 255 FLAAL+ I+PSLYEAA VDGA RW+QTIY LGN+LNA FDQ+ N Sbjct: 196 FLAALTSINPSLYEAAIVDGANRWQQTIYITLPSMLPIIIVVGTLSLGNILNAGFDQILN 255 Query: 256 LYSPLIYQQGDIIDTFVYREGLLSGQFSFATAVNLFKSAISLILIVISYRLAYRFAGYRI 315 LY+PL+Y+ GDIIDT+VYR GLL+G FS+ATAV LFKS IS IL+V YRLAY++A Y+I Sbjct: 256 LYNPLVYKTGDIIDTYVYRVGLLNGDFSYATAVGLFKSIISFILVVTGYRLAYKYANYKI 315 Query: 316 F 316 F Sbjct: 316 F 316 >C3J6V5.1 C3J6V5_9BACI Binding-protein-dependent transport systems inner membrane component Length = 315 Score = 364 bits (934), Expect = 7e-99, Method: Compositional matrix adjust. Identities = 177/303 (58%), Positives = 225/303 (74%), Gaps = 2/303 (0%) Query: 15 LRQSRKKKVKQPWMLHLMVLPAAVMVFIFSYIPMGGILMAFQDYKPKPGLFGSPWVGLKH 74 + R +K K W LH+M++PA ++V IF Y+PM G++MAFQDY+P G SPWVGL+H Sbjct: 14 VETKRVQKRKGTWQLHVMLIPALIIVLIFQYVPMLGLVMAFQDYEPWLGFLHSPWVGLEH 73 Query: 75 FEYMWQNDYFLQITWNTLFFAGTKIIMNLIIPFFFALLLNEVRKMALKRTIQTLVYLPHF 134 F+ M++ + Q+ WNTL + KII NL +P FALLLNEV M KRT+QT+VYLPHF Sbjct: 74 FKTMFEYEDARQVIWNTLVISTLKIIFNLGVPLAFALLLNEVYVMKFKRTVQTIVYLPHF 133 Query: 135 LSWVTLSGILIDMLA-QTGLINQFISSVFGIKPIFFLGDGSWFRFTIIFSDVWKEFGFNM 193 LSWV L GILIDML+ + G++N+F+ SVFG++PIFFLGD WFR ++ +DVWKE GFN Sbjct: 134 LSWVILGGILIDMLSPEGGIVNRFL-SVFGVEPIFFLGDNDWFRTIVVVTDVWKECGFNT 192 Query: 194 IIFLAALSGISPSLYEAAEVDGAGRWKQTIYXXXXXXXXXXXXXXXXXLGNVLNANFDQV 253 I+FLAAL+ I+PSLYEAA VDGA RW+QTIY LGN+LNA FDQ+ Sbjct: 193 IVFLAALTSINPSLYEAAIVDGANRWQQTIYITLPSMLPIIIVVGTLSLGNILNAGFDQI 252 Query: 254 FNLYSPLIYQQGDIIDTFVYREGLLSGQFSFATAVNLFKSAISLILIVISYRLAYRFAGY 313 NLY+PL+Y+ GDIIDT+VYR GLL+G FS+ATAV LFKS IS IL+V YRLAY++A Y Sbjct: 253 LNLYNPLVYKTGDIIDTYVYRVGLLNGDFSYATAVGLFKSVISFILVVTGYRLAYKYANY 312 Query: 314 RIF 316 +IF Sbjct: 313 KIF 315 >B7YFA2.1 B7YFA2_9BACI Binding-protein-dependent transport systems inner membrane component Length = 315 Score = 364 bits (934), Expect = 7e-99, Method: Compositional matrix adjust. Identities = 177/303 (58%), Positives = 225/303 (74%), Gaps = 2/303 (0%) Query: 15 LRQSRKKKVKQPWMLHLMVLPAAVMVFIFSYIPMGGILMAFQDYKPKPGLFGSPWVGLKH 74 + R +K K W LH+M++PA ++V IF Y+PM G++MAFQDY+P G SPWVGL+H Sbjct: 14 VETKRVQKRKGTWQLHVMLIPALIIVLIFQYVPMLGLVMAFQDYEPWLGFLHSPWVGLEH 73 Query: 75 FEYMWQNDYFLQITWNTLFFAGTKIIMNLIIPFFFALLLNEVRKMALKRTIQTLVYLPHF 134 F+ M++ + Q+ WNTL + KII NL +P FALLLNEV M KRT+QT+VYLPHF Sbjct: 74 FKTMFEYEDARQVIWNTLVISTLKIIFNLGVPLAFALLLNEVYVMKFKRTVQTIVYLPHF 133 Query: 135 LSWVTLSGILIDMLA-QTGLINQFISSVFGIKPIFFLGDGSWFRFTIIFSDVWKEFGFNM 193 LSWV L GILIDML+ + G++N+F+ SVFG++PIFFLGD WFR ++ +DVWKE GFN Sbjct: 134 LSWVILGGILIDMLSPEGGIVNRFL-SVFGVEPIFFLGDNDWFRTIVVVTDVWKECGFNT 192 Query: 194 IIFLAALSGISPSLYEAAEVDGAGRWKQTIYXXXXXXXXXXXXXXXXXLGNVLNANFDQV 253 I+FLAAL+ I+PSLYEAA VDGA RW+QTIY LGN+LNA FDQ+ Sbjct: 193 IVFLAALTSINPSLYEAAIVDGANRWQQTIYITLPSMLPIIIVVGTLSLGNILNAGFDQI 252 Query: 254 FNLYSPLIYQQGDIIDTFVYREGLLSGQFSFATAVNLFKSAISLILIVISYRLAYRFAGY 313 NLY+PL+Y+ GDIIDT+VYR GLL+G FS+ATAV LFKS IS IL+V YRLAY++A Y Sbjct: 253 LNLYNPLVYKTGDIIDTYVYRVGLLNGDFSYATAVGLFKSVISFILVVTGYRLAYKYANY 312 Query: 314 RIF 316 +IF Sbjct: 313 KIF 315 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.144 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,776,629,120 Number of extensions: 71508496 Number of successful extensions: 210801 Number of sequences better than 1.0e-05: 4369 Number of HSP's gapped: 207877 Number of HSP's successfully gapped: 4370 Length of query: 316 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 181 Effective length of database: 1,788,859,178 Effective search space: 323783511218 Effective search space used: 323783511218 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)