BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0028.p (222 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIX3.1 B3KIX3_9BACI SNARE associated Golgi protein 224 5e-57 Q8ERW8.1 Q8ERW8_OCEIH Hypothetical conserved protein 130 8e-29 C2W505.1 C2W505_BACCE SNARE associated Golgi protein 127 8e-28 C3B0C6.1 C3B0C6_BACMY SNARE associated Golgi protein 125 3e-27 C3AIL7.1 C3AIL7_BACMY SNARE associated Golgi protein 125 3e-27 >B3KIX3.1 B3KIX3_9BACI SNARE associated Golgi protein Length = 214 Score = 224 bits (570), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 106/202 (52%), Positives = 146/202 (72%) Query: 11 FLDSMSWXXXXXXXXXXXXXXXXGPLPGIGLTFMKSFVPPLPTIAIVGLNGAVYGLWLGF 70 +LD MS+ GPLPGI LTF+KSF+PP+PTI IVG+N AVYGLW GF Sbjct: 7 YLDIMSYFTQSNLEWFLDQFRALGPLPGILLTFLKSFIPPMPTIVIVGVNAAVYGLWAGF 66 Query: 71 LYSWLGLVAGCVTTFLIIRQIASHPYLRKWALRPKVARGMQWVRNSGFSYVFLLSLFPVG 130 LYSW+G+VAGC+TTFL++R+I+ P + +WA +PKV RGM+W++ +GFSYVFLL + PVG Sbjct: 67 LYSWIGMVAGCLTTFLLVRKISGTPLIERWAKKPKVQRGMRWIQRNGFSYVFLLGILPVG 126 Query: 131 PFVVINMAAGLAQMRLRSYLLALCAGKAIMVFAVSYIGNDMNRFTRHPGEIIYVLLFIGG 190 PFV +N+AAGLA+M + SYL+A+ GK IMVF VSYIG +++ F P +++ VL FI Sbjct: 127 PFVAVNVAAGLARMNVMSYLMAVTLGKGIMVFCVSYIGANLSDFMAEPFKLLGVLAFILL 186 Query: 191 SLWGVKAIEARFTRAAQAREKQ 212 SLWG + +++ F R +++ Sbjct: 187 SLWGSRKLDSYFVRKQSFHDQE 208 >Q8ERW8.1 Q8ERW8_OCEIH Hypothetical conserved protein Length = 212 Score = 130 bits (327), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 61/145 (42%), Positives = 95/145 (65%) Query: 34 GPLPGIGLTFMKSFVPPLPTIAIVGLNGAVYGLWLGFLYSWLGLVAGCVTTFLIIRQIAS 93 GPLPGI L F+++F+P LP I V N A YGL+ GFLYSW+G G + FL+IR++ + Sbjct: 35 GPLPGILLPFLEAFLPFLPLIVFVLGNAAAYGLFQGFLYSWIGATVGAIVVFLLIRKLVN 94 Query: 94 HPYLRKWALRPKVARGMQWVRNSGFSYVFLLSLFPVGPFVVINMAAGLAQMRLRSYLLAL 153 L+K +V + QWV GF +F+L FP P +IN+ AGL+++ + ++LA+ Sbjct: 95 TRLLQKIRKNSQVKKITQWVDKHGFGPLFILLCFPFSPSSIINVVAGLSKINMYQFILAV 154 Query: 154 CAGKAIMVFAVSYIGNDMNRFTRHP 178 C GK +M+F++SY+G+ + F ++P Sbjct: 155 CLGKTVMIFSISYVGSSIMEFAQNP 179 >C2W505.1 C2W505_BACCE SNARE associated Golgi protein Length = 197 Score = 127 bits (318), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 71/171 (41%), Positives = 101/171 (59%), Gaps = 6/171 (3%) Query: 34 GPLPGIGLTFMKSFVPPLPTIAIVGLNGAVYGLWLGFLYSWLGLVAGCVTTFLIIRQIAS 93 GPL GIGL +++ +P LP I V N +GLW GFLYSW+G V G + F +IR+ Sbjct: 26 GPLLGIGLPMVEAIIPALPLIVFVMANAVAFGLWFGFLYSWIGSVLGALIVFSVIRRFGR 85 Query: 94 HPYLRKWALRPKVARGMQWVRNSGFSYVFLLSLFPVGPFVVINMAAGLAQMRLRSYLLAL 153 + PKV + M W+ GF+ +F+L FP P +IN+ AGL+++ + ++LAL Sbjct: 86 TRFFSFVNKHPKVRKAMGWIERKGFAPIFILFCFPFTPSALINVVAGLSRISRKQFVLAL 145 Query: 154 CAGKAIMVFAVSYIGNDMNRFTRHPGEIIYVL--LFIGGSLWGV-KAIEAR 201 GK +MVF +SYIG+D+ F P + I VL +FI LW V K IE + Sbjct: 146 ALGKLVMVFILSYIGSDLTSFIYKPVKTIIVLGVIFI---LWYVGKKIEVK 193 >C3B0C6.1 C3B0C6_BACMY SNARE associated Golgi protein Length = 197 Score = 125 bits (314), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 69/171 (40%), Positives = 100/171 (58%), Gaps = 6/171 (3%) Query: 34 GPLPGIGLTFMKSFVPPLPTIAIVGLNGAVYGLWLGFLYSWLGLVAGCVTTFLIIRQIAS 93 GPL GI L +++ +P LP I V N +G WLGFLYSWLG V G + F +IR+ Sbjct: 26 GPLLGISLPMVEALIPALPLILFVMANAVAFGFWLGFLYSWLGSVLGALIVFSVIRRFGR 85 Query: 94 HPYLRKWALRPKVARGMQWVRNSGFSYVFLLSLFPVGPFVVINMAAGLAQMRLRSYLLAL 153 + PKV + M W+ GF+ +F+L FP P +IN+ AGL+++ R ++LAL Sbjct: 86 SRFFSFVNKHPKVRKAMGWIERKGFAPIFILFCFPFTPSALINVVAGLSRISRRQFVLAL 145 Query: 154 CAGKAIMVFAVSYIGNDMNRFTRHPGEIIYVL--LFIGGSLWGV-KAIEAR 201 GK++M+ +SYIG+D+ F P + I V+ +FI LW V K IE + Sbjct: 146 ALGKSVMILILSYIGHDLTSFIHKPVKTIIVIGVIFI---LWYVGKKIEVK 193 >C3AIL7.1 C3AIL7_BACMY SNARE associated Golgi protein Length = 197 Score = 125 bits (314), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 69/171 (40%), Positives = 100/171 (58%), Gaps = 6/171 (3%) Query: 34 GPLPGIGLTFMKSFVPPLPTIAIVGLNGAVYGLWLGFLYSWLGLVAGCVTTFLIIRQIAS 93 GPL GI L +++ +P LP I V N +G WLGFLYSWLG V G + F +IR+ Sbjct: 26 GPLLGISLPMVEALIPALPLILFVMANAVAFGFWLGFLYSWLGSVLGALIVFSVIRRFGR 85 Query: 94 HPYLRKWALRPKVARGMQWVRNSGFSYVFLLSLFPVGPFVVINMAAGLAQMRLRSYLLAL 153 + PKV + M W+ GF+ +F+L FP P +IN+ AGL+++ R ++LAL Sbjct: 86 SRFFSFVNKHPKVRKAMGWIERKGFAPIFILFCFPFTPSALINVVAGLSRISRRQFVLAL 145 Query: 154 CAGKAIMVFAVSYIGNDMNRFTRHPGEIIYVL--LFIGGSLWGV-KAIEAR 201 GK++M+ +SYIG+D+ F P + I V+ +FI LW V K IE + Sbjct: 146 ALGKSVMILILSYIGHDLTSFIHKPVKTIIVIGVIFI---LWYVGKKIEVK 193 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.143 0.460 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,236,439,388 Number of extensions: 46152890 Number of successful extensions: 139525 Number of sequences better than 1.0e-05: 299 Number of HSP's gapped: 139957 Number of HSP's successfully gapped: 299 Length of query: 222 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 91 Effective length of database: 1,826,543,238 Effective search space: 166215434658 Effective search space used: 166215434658 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)