BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0016.p (490 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5KYK0.2 IOLA3_GEOKA Methylmalonate semialdehyde dehydrogenase [... 774 0.0 C3J4C3.1 C3J4C3_9BACI Methylmalonate-semialdehyde dehydrogenase 772 0.0 B7YEI0.1 B7YEI0_9BACI Methylmalonate-semialdehyde dehydrogenase 772 0.0 B4BM53.1 B4BM53_9BACI Methylmalonate-semialdehyde dehydrogenase 771 0.0 A4IPF5.2 IOLA2_GEOTN Methylmalonate semialdehyde dehydrogenase [... 769 0.0 >Q5KYK0.2 IOLA3_GEOKA Methylmalonate semialdehyde dehydrogenase [acylating] 3 EC=1.2.1.27 EC=1.2.1.18 Length = 484 Score = 774 bits (1999), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/483 (76%), Positives = 423/483 (87%) Query: 8 VKQTEKVKNYVNGVWVDSSSGQQEEVFNPATGEVIAYVPISSRDELNDAVKAAAEAYRSW 67 + +T+ +KN++ G WV S+SG++E V NPATGEV+A VP+SSR+EL+ AV AA EA+R W Sbjct: 1 MTETKTLKNFIGGQWVASTSGKEEVVPNPATGEVLAKVPLSSREELDAAVAAAKEAFREW 60 Query: 68 KKVAVPRRARYFFQYQQLLAQHRNELAGLITLENGKSLEEALGEVQRGIECVEFAAGIPT 127 +KV VPRRAR F+YQQLL +H +ELA L+TLENGK E+A GEVQRGIECVEFAAGIPT Sbjct: 61 RKVPVPRRARILFRYQQLLVEHWDELARLVTLENGKVYEDAYGEVQRGIECVEFAAGIPT 120 Query: 128 LMMGSQLPDIASGVESGMYRYPLGVVGGIAPFNFPMMVPCWMFPLAVACGNTFVLKPSER 187 LMMG QLPDIA+ +ESGMYRYPLGVV GI PFNFPMMVPCWMFPLA+ACGNTFVLKPSER Sbjct: 121 LMMGQQLPDIATDIESGMYRYPLGVVAGITPFNFPMMVPCWMFPLAIACGNTFVLKPSER 180 Query: 188 TPLLMNRLAELFAEAGFPPGVLNVVHGAHEVVNGLLDHEEVKAVSFVGSQPVAEYVYKRG 247 TP+L NRLAELF EAG PPGVLN+VHGAHEVV G+L+H+++KAVSFVGSQPVAEYVYK Sbjct: 181 TPMLANRLAELFTEAGLPPGVLNIVHGAHEVVGGILEHKDIKAVSFVGSQPVAEYVYKTA 240 Query: 248 TSSGKRVQALAGAKNHSIVLPDADLGSAVKNIISAAFGSAGERCMACSVVVAHEAVADEL 307 + GKRVQALAGAKNHSIV+PDADL AV NII+AAFGSAGERCMACSVVVA +ADEL Sbjct: 241 AAHGKRVQALAGAKNHSIVMPDADLDMAVTNIINAAFGSAGERCMACSVVVAVGDIADEL 300 Query: 308 VRRLVEAADSLKIGDGKDEGVFLGPVIRQSNKERTAAYIETGIQEQAELVRDGRLDSAAG 367 VRRL EAAD ++IG+G D+GVFLGPVIR+S+KERT YIE G +E A LVRDGR DSA Sbjct: 301 VRRLKEAADRIQIGNGLDKGVFLGPVIRESHKERTIKYIEIGEKEGALLVRDGRRDSATS 360 Query: 368 GAGYFLGPTLFDHVKPGMTIWREEIFAPLLAVVRVKDLAEAIAVTNQSPFANGACIYTDS 427 G GYF+GPT+FDHVKPGMTIW +EIFAP+L+VVR +DL EAI + N+S FANGACIYTDS Sbjct: 361 GQGYFIGPTIFDHVKPGMTIWTDEIFAPVLSVVRARDLDEAIEIANRSEFANGACIYTDS 420 Query: 428 AKAIREFREEIDAGMLGINLGVPAPMAFFPFSGYKKSFYGDLHANGRDGVEFYTRKKMVT 487 AKAIR+FREEIDAGMLG+N+ VPAPMAFFPFSGYK SFYGDLHANGRDGVEFYTRKKMVT Sbjct: 421 AKAIRQFREEIDAGMLGVNVAVPAPMAFFPFSGYKNSFYGDLHANGRDGVEFYTRKKMVT 480 Query: 488 ARY 490 ARY Sbjct: 481 ARY 483 >C3J4C3.1 C3J4C3_9BACI Methylmalonate-semialdehyde dehydrogenase Length = 486 Score = 772 bits (1994), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/483 (76%), Positives = 421/483 (87%) Query: 8 VKQTEKVKNYVNGVWVDSSSGQQEEVFNPATGEVIAYVPISSRDELNDAVKAAAEAYRSW 67 V +T+ +KN++ G WV S+SG++E V NPATGEV+ VP+SSR+EL+ AV AA EA+R W Sbjct: 4 VTETKTLKNFIGGQWVTSTSGKEEIVPNPATGEVLMKVPLSSREELDAAVAAAKEAFREW 63 Query: 68 KKVAVPRRARYFFQYQQLLAQHRNELAGLITLENGKSLEEALGEVQRGIECVEFAAGIPT 127 +KV VPRRA F+YQQLL +H ELA L+TLENGK E+A GEVQRGIECVEFAAGIPT Sbjct: 64 RKVPVPRRAHILFRYQQLLVEHWEELARLVTLENGKVYEDAYGEVQRGIECVEFAAGIPT 123 Query: 128 LMMGSQLPDIASGVESGMYRYPLGVVGGIAPFNFPMMVPCWMFPLAVACGNTFVLKPSER 187 LMMG QLPDIA+G+ESGMYRYPLGVV GI PFNFPMMVPCWMFPLA+ACGNTFVLKPSER Sbjct: 124 LMMGQQLPDIATGIESGMYRYPLGVVAGITPFNFPMMVPCWMFPLAIACGNTFVLKPSER 183 Query: 188 TPLLMNRLAELFAEAGFPPGVLNVVHGAHEVVNGLLDHEEVKAVSFVGSQPVAEYVYKRG 247 TP+L NRLAELF EAG PPGVLN+VHGAHEVV G+L+H+++KAVSFVGSQPVAEYVYK Sbjct: 184 TPMLANRLAELFTEAGLPPGVLNIVHGAHEVVGGILEHKDIKAVSFVGSQPVAEYVYKTA 243 Query: 248 TSSGKRVQALAGAKNHSIVLPDADLGSAVKNIISAAFGSAGERCMACSVVVAHEAVADEL 307 + GKRVQALAGAKNHSIV+PDADL AV NII+AAFGSAGERCMACSVVVA +ADEL Sbjct: 244 AAHGKRVQALAGAKNHSIVMPDADLDMAVTNIINAAFGSAGERCMACSVVVAVGDIADEL 303 Query: 308 VRRLVEAADSLKIGDGKDEGVFLGPVIRQSNKERTAAYIETGIQEQAELVRDGRLDSAAG 367 V+RL EAAD ++IG+G D+GVFLGPVIR+S+KERT YIE G +E A LVRDGR DSA Sbjct: 304 VKRLKEAADRIQIGNGLDQGVFLGPVIRESHKERTIKYIEIGEKEGALLVRDGRRDSATS 363 Query: 368 GAGYFLGPTLFDHVKPGMTIWREEIFAPLLAVVRVKDLAEAIAVTNQSPFANGACIYTDS 427 G GYF+GPT+FDHVKPGMTIW +EIFAP+L+VVR +DL EAI + N+S FANGACIYTDS Sbjct: 364 GKGYFIGPTIFDHVKPGMTIWTDEIFAPVLSVVRARDLDEAIEIANRSEFANGACIYTDS 423 Query: 428 AKAIREFREEIDAGMLGINLGVPAPMAFFPFSGYKKSFYGDLHANGRDGVEFYTRKKMVT 487 AKAIR+FREEIDAGMLG+N+ VPAPMAFFPFSGYK SFYGDLHANGRDGVEFYTRKKMVT Sbjct: 424 AKAIRQFREEIDAGMLGVNVAVPAPMAFFPFSGYKNSFYGDLHANGRDGVEFYTRKKMVT 483 Query: 488 ARY 490 ARY Sbjct: 484 ARY 486 >B7YEI0.1 B7YEI0_9BACI Methylmalonate-semialdehyde dehydrogenase Length = 486 Score = 772 bits (1994), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/483 (76%), Positives = 421/483 (87%) Query: 8 VKQTEKVKNYVNGVWVDSSSGQQEEVFNPATGEVIAYVPISSRDELNDAVKAAAEAYRSW 67 V +T+ +KN++ G WV S+SG++E V NPATGEV+ VP+SSR+EL+ AV AA EA+R W Sbjct: 4 VTETKTLKNFIGGQWVTSTSGKEEIVPNPATGEVLMKVPLSSREELDAAVAAAKEAFREW 63 Query: 68 KKVAVPRRARYFFQYQQLLAQHRNELAGLITLENGKSLEEALGEVQRGIECVEFAAGIPT 127 +KV VPRRA F+YQQLL +H ELA L+TLENGK E+A GEVQRGIECVEFAAGIPT Sbjct: 64 RKVPVPRRAHILFRYQQLLVEHWEELARLVTLENGKVYEDAYGEVQRGIECVEFAAGIPT 123 Query: 128 LMMGSQLPDIASGVESGMYRYPLGVVGGIAPFNFPMMVPCWMFPLAVACGNTFVLKPSER 187 LMMG QLPDIA+G+ESGMYRYPLGVV GI PFNFPMMVPCWMFPLA+ACGNTFVLKPSER Sbjct: 124 LMMGQQLPDIATGIESGMYRYPLGVVAGITPFNFPMMVPCWMFPLAIACGNTFVLKPSER 183 Query: 188 TPLLMNRLAELFAEAGFPPGVLNVVHGAHEVVNGLLDHEEVKAVSFVGSQPVAEYVYKRG 247 TP+L NRLAELF EAG PPGVLN+VHGAHEVV G+L+H+++KAVSFVGSQPVAEYVYK Sbjct: 184 TPMLANRLAELFTEAGLPPGVLNIVHGAHEVVGGILEHKDIKAVSFVGSQPVAEYVYKTA 243 Query: 248 TSSGKRVQALAGAKNHSIVLPDADLGSAVKNIISAAFGSAGERCMACSVVVAHEAVADEL 307 + GKRVQALAGAKNHSIV+PDADL AV NII+AAFGSAGERCMACSVVVA +ADEL Sbjct: 244 AAHGKRVQALAGAKNHSIVMPDADLDMAVTNIINAAFGSAGERCMACSVVVAVGDIADEL 303 Query: 308 VRRLVEAADSLKIGDGKDEGVFLGPVIRQSNKERTAAYIETGIQEQAELVRDGRLDSAAG 367 V+RL EAAD ++IG+G D+GVFLGPVIR+S+KERT YIE G +E A LVRDGR DSA Sbjct: 304 VKRLKEAADRIQIGNGLDQGVFLGPVIRESHKERTIKYIEIGEKEGALLVRDGRRDSATS 363 Query: 368 GAGYFLGPTLFDHVKPGMTIWREEIFAPLLAVVRVKDLAEAIAVTNQSPFANGACIYTDS 427 G GYF+GPT+FDHVKPGMTIW +EIFAP+L+VVR +DL EAI + N+S FANGACIYTDS Sbjct: 364 GKGYFIGPTIFDHVKPGMTIWTDEIFAPVLSVVRARDLDEAIEIANRSEFANGACIYTDS 423 Query: 428 AKAIREFREEIDAGMLGINLGVPAPMAFFPFSGYKKSFYGDLHANGRDGVEFYTRKKMVT 487 AKAIR+FREEIDAGMLG+N+ VPAPMAFFPFSGYK SFYGDLHANGRDGVEFYTRKKMVT Sbjct: 424 AKAIRQFREEIDAGMLGVNVAVPAPMAFFPFSGYKNSFYGDLHANGRDGVEFYTRKKMVT 483 Query: 488 ARY 490 ARY Sbjct: 484 ARY 486 >B4BM53.1 B4BM53_9BACI Methylmalonate-semialdehyde dehydrogenase Length = 486 Score = 771 bits (1991), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/483 (76%), Positives = 422/483 (87%) Query: 8 VKQTEKVKNYVNGVWVDSSSGQQEEVFNPATGEVIAYVPISSRDELNDAVKAAAEAYRSW 67 V +T+ +KN++ G WV S+SG++E V NPATGEV+A VPISSR+EL+ AV AA EA+R W Sbjct: 4 VTETKTLKNFIGGQWVASTSGKEEIVPNPATGEVLAKVPISSREELDAAVAAAKEAFREW 63 Query: 68 KKVAVPRRARYFFQYQQLLAQHRNELAGLITLENGKSLEEALGEVQRGIECVEFAAGIPT 127 +KV VPRRAR F+YQQLL +H ELA L+TLENGK E+A GEVQRGIECVEFAAGIPT Sbjct: 64 RKVPVPRRARILFRYQQLLVEHWEELARLVTLENGKVYEDAYGEVQRGIECVEFAAGIPT 123 Query: 128 LMMGSQLPDIASGVESGMYRYPLGVVGGIAPFNFPMMVPCWMFPLAVACGNTFVLKPSER 187 LMMG QLPDIA+G+ESGMYRYPLGVV GI PFNFPMMVPCWMFPLA+ACGNTFVLKPSER Sbjct: 124 LMMGQQLPDIATGIESGMYRYPLGVVAGITPFNFPMMVPCWMFPLAIACGNTFVLKPSER 183 Query: 188 TPLLMNRLAELFAEAGFPPGVLNVVHGAHEVVNGLLDHEEVKAVSFVGSQPVAEYVYKRG 247 TPLL NRLAELF EAG P GVLN+VHGAHEVVNG+L+H+++KAVSFVGSQPVAEYVYK Sbjct: 184 TPLLANRLAELFTEAGLPAGVLNIVHGAHEVVNGILEHKDIKAVSFVGSQPVAEYVYKTA 243 Query: 248 TSSGKRVQALAGAKNHSIVLPDADLGSAVKNIISAAFGSAGERCMACSVVVAHEAVADEL 307 + GKRVQALAGAKNHSIV+PDADL AV NII+AAFGSAGERCMACSVVVA +ADEL Sbjct: 244 AAYGKRVQALAGAKNHSIVMPDADLDMAVTNIINAAFGSAGERCMACSVVVAVGDIADEL 303 Query: 308 VRRLVEAADSLKIGDGKDEGVFLGPVIRQSNKERTAAYIETGIQEQAELVRDGRLDSAAG 367 V RL +AAD ++IG+G D+GVFLGPVIR+S+KERT YIE G +E A LVRDGR D+A Sbjct: 304 VERLKKAADRIQIGNGLDQGVFLGPVIRESHKERTIKYIEIGEREGALLVRDGRRDAATS 363 Query: 368 GAGYFLGPTLFDHVKPGMTIWREEIFAPLLAVVRVKDLAEAIAVTNQSPFANGACIYTDS 427 G GYF+GPT+FDHVKPGMTIW +EIFAP+L+VVR +DL EAI + N+S FANGACIYTDS Sbjct: 364 GKGYFVGPTIFDHVKPGMTIWTDEIFAPVLSVVRARDLDEAIEIANRSEFANGACIYTDS 423 Query: 428 AKAIREFREEIDAGMLGINLGVPAPMAFFPFSGYKKSFYGDLHANGRDGVEFYTRKKMVT 487 AKAIR+FREEIDAGMLG+N+ VPAPMAFFPFSGYK SFYGDLHANGRDGVEFYTRKKMVT Sbjct: 424 AKAIRQFREEIDAGMLGVNVAVPAPMAFFPFSGYKNSFYGDLHANGRDGVEFYTRKKMVT 483 Query: 488 ARY 490 AR+ Sbjct: 484 ARH 486 >A4IPF5.2 IOLA2_GEOTN Methylmalonate semialdehyde dehydrogenase [acylating] 2 EC=1.2.1.27 EC=1.2.1.18 Length = 483 Score = 770 bits (1987), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/483 (76%), Positives = 422/483 (87%) Query: 8 VKQTEKVKNYVNGVWVDSSSGQQEEVFNPATGEVIAYVPISSRDELNDAVKAAAEAYRSW 67 + +T+ +KN++ G WV S+SG++E V NPATGEV+A VPISSR+EL+ AV AA EA+R W Sbjct: 1 MTETKTLKNFIGGQWVASTSGKEEIVPNPATGEVLAKVPISSREELDAAVAAAKEAFREW 60 Query: 68 KKVAVPRRARYFFQYQQLLAQHRNELAGLITLENGKSLEEALGEVQRGIECVEFAAGIPT 127 +KV VPRRAR F+YQQLL +H ELA L+TLENGK E+A GEVQRGIECVEFAAGIPT Sbjct: 61 RKVPVPRRARILFRYQQLLVEHWEELARLVTLENGKVYEDAYGEVQRGIECVEFAAGIPT 120 Query: 128 LMMGSQLPDIASGVESGMYRYPLGVVGGIAPFNFPMMVPCWMFPLAVACGNTFVLKPSER 187 LMMG QLPDIA+G+ESGMYRYPLGVV GI PFNFPMMVPCWMFPLA+ACGNTFVLKPSER Sbjct: 121 LMMGQQLPDIATGIESGMYRYPLGVVAGITPFNFPMMVPCWMFPLAIACGNTFVLKPSER 180 Query: 188 TPLLMNRLAELFAEAGFPPGVLNVVHGAHEVVNGLLDHEEVKAVSFVGSQPVAEYVYKRG 247 TPLL NRLAELF EAG P GVLN+VHGAHEVVNG+L+H+++KAVSFVGSQPVAEYVYK Sbjct: 181 TPLLANRLAELFTEAGLPAGVLNIVHGAHEVVNGILEHKDIKAVSFVGSQPVAEYVYKTA 240 Query: 248 TSSGKRVQALAGAKNHSIVLPDADLGSAVKNIISAAFGSAGERCMACSVVVAHEAVADEL 307 + GKRVQALAGAKNHSIV+PDADL AV NII+AAFGSAGERCMACSVVVA +ADEL Sbjct: 241 AAYGKRVQALAGAKNHSIVMPDADLDMAVTNIINAAFGSAGERCMACSVVVAVGDIADEL 300 Query: 308 VRRLVEAADSLKIGDGKDEGVFLGPVIRQSNKERTAAYIETGIQEQAELVRDGRLDSAAG 367 V RL +AAD ++IG+G D+GVFLGPVIR+S+KERT YIE G +E A LVRDGR D+A Sbjct: 301 VERLKKAADRIQIGNGLDQGVFLGPVIRESHKERTIKYIEIGEREGALLVRDGRRDAATS 360 Query: 368 GAGYFLGPTLFDHVKPGMTIWREEIFAPLLAVVRVKDLAEAIAVTNQSPFANGACIYTDS 427 G GYF+GPT+FDHVKPGMTIW +EIFAP+L+VVR +DL EAI + N+S FANGACIYTDS Sbjct: 361 GKGYFVGPTIFDHVKPGMTIWTDEIFAPVLSVVRARDLDEAIEIANRSEFANGACIYTDS 420 Query: 428 AKAIREFREEIDAGMLGINLGVPAPMAFFPFSGYKKSFYGDLHANGRDGVEFYTRKKMVT 487 AKAIR+FREEIDAGMLG+N+ VPAPMAFFPFSGYK SFYGDLHANGRDGVEFYTRKKMVT Sbjct: 421 AKAIRQFREEIDAGMLGVNVAVPAPMAFFPFSGYKNSFYGDLHANGRDGVEFYTRKKMVT 480 Query: 488 ARY 490 AR+ Sbjct: 481 ARH 483 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,913,706,843 Number of extensions: 124127958 Number of successful extensions: 372955 Number of sequences better than 1.0e-05: 13029 Number of HSP's gapped: 336460 Number of HSP's successfully gapped: 13145 Length of query: 490 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 350 Effective length of database: 1,741,754,103 Effective search space: 609613936050 Effective search space used: 609613936050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)