BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0009.p (530 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5W9.1 B3K5W9_9BACI Diguanylate cyclase 450 e-124 B1DCR2.1 B1DCR2_9BACL Diguanylate cyclase with PAS/PAC sensor 450 e-124 Q2B6Y4.1 Q2B6Y4_9BACI Putative uncharacterized protein 438 e-121 B4AGE7.1 B4AGE7_BACPU Putative diguanylate cyclase 384 e-104 A8FHG3.1 A8FHG3_BACP2 Possible diguanylate cyclase 380 e-103 >B3K5W9.1 B3K5W9_9BACI Diguanylate cyclase Length = 539 Score = 450 bits (1158), Expect = e-124, Method: Compositional matrix adjust. Identities = 237/538 (44%), Positives = 337/538 (62%), Gaps = 19/538 (3%) Query: 1 MGSMISNYIVIVSISGVLSALLALFAYYKKTDFAGTRAFILSSLTSAIYTFGFALELSGS 60 MG+ S I ++ +GVL+ ++ L+ ++ A + F + S+ + IYTFG ALELS Sbjct: 1 MGTPYSAVITLIVTAGVLNIIMGLYVLSGRSKLATVKTFFIFSMLAPIYTFGAALELSSR 60 Query: 61 TMPEISFWIKVEYLGMPFIAPSSLLMIMYFVGLERLIRKGLVTALYAIPFISVLLVWTND 120 ++ EI FWIK EYLGMPF+ P +LL+I+ ++G+ER +R GL+ +++ IP +++LLV TN+ Sbjct: 61 SLQEIQFWIKFEYLGMPFLPPLNLLIILTYLGMERYLRPGLLISMFVIPVVTLLLVMTNE 120 Query: 121 YHHLFYQSMYFREGAPTPLIDVVMGPWYIVQGSLTFGCMLAGMCLILWQWNRMKRVYRRQ 180 HHL+Y+ + R +D+V GPWYI+QG+ TF C++ G+ L+L W +M YR Q Sbjct: 121 RHHLYYRDILLRPDVSVLKVDIVAGPWYIIQGAYTFACLIGGVVLLLRSWRKMPS-YRFQ 179 Query: 181 LFTIFIGQFLPALGAFLYLMDVTPYGMDPVPVIMSITSTLYIWAILSRGMLTAAPIAREN 240 L T+ +G LP +G FLYL +TP GMDP+PVIM++TS LY+WA+ S+G+ APIAR+N Sbjct: 180 LGTMLVGLVLPLIGDFLYLGGLTPDGMDPIPVIMTLTSVLYLWALTSKGLFHVAPIARDN 239 Query: 241 LFESMRDGVLVTDLSDMLVDYNRAAADMLQDLDASAIGKPLAQLF-LPAGQDAVDYVMNS 299 LF SMR+GVLV DL + LVD+N AAA M+ +L SAIG+PL +++ L + Sbjct: 240 LFSSMREGVLVLDLDNRLVDFNPAAATMMPELSPSAIGEPLHEMWQLHTAEPIFGMGERE 299 Query: 300 DPLISEE--RELGWNMNGETRYYQIRSSPVQKYGGHQVGRMIMLIDVTERTLLQEKLLQL 357 P E E+ W + + +YQIRSS V+ G + GR+I+LIDVT++ LQE+L Q+ Sbjct: 300 GPDTGEADIEEIRWQLGNHSLHYQIRSSTVRNQRGREAGRLIVLIDVTDKARLQEELRQM 359 Query: 358 ATVDSLTGIYNRMHFMELSRGLLNEAALSLAPVSIILLDIDFFKSINDRYGHHYGDMALQ 417 A D LT IYNR HFM+LS LLN+A + P++ +L DID FK IND YGH GD ALQ Sbjct: 360 AYYDGLTRIYNRSHFMKLSYALLNQAVRNREPLAFLLFDIDHFKRINDVYGHDIGDQALQ 419 Query: 418 HIVTVCSSHLREGDIFGRYGGEEFVLCLPETSLKQAALLXXXXXXXXXXXXXXXXXGPIH 477 HIV + LR D+F RYGGEEF + +P S +A G + Sbjct: 420 HIVELTRKTLRAEDLFARYGGEEFAVAMPGQSTSEATAAAERIRAKLLAQPMTTPQGSLM 479 Query: 478 VTASFGV-----------VEARGK----NLSLEDLLSEADHALYTSKRNGRNAVHVSS 520 +TASFGV V+A + + L+ +L +AD ALY +KR+GRN + VS Sbjct: 480 ITASFGVAAVKTSSHNGTVDAEAEEAWWDTELKKVLKQADRALYEAKRSGRNTIRVSD 537 >B1DCR2.1 B1DCR2_9BACL Diguanylate cyclase with PAS/PAC sensor Length = 538 Score = 450 bits (1157), Expect = e-124, Method: Compositional matrix adjust. Identities = 237/536 (44%), Positives = 337/536 (62%), Gaps = 15/536 (2%) Query: 1 MGSMISNYIVIVSISGVLSALLALFAYYKKTDFAGTRAFILSSLTSAIYTFGFALELSGS 60 MG+ S + ++ +GV++ ++ ++ + ++ A R FI SAIY FG ALELS Sbjct: 1 MGTPYSAALTLMVTAGVINIIMGIYVFMNRSKQAMARTFIAMCFLSAIYIFGSALELSAG 60 Query: 61 TMPEISFWIKVEYLGMPFIAPSSLLMIMYFVGLERLIRKGLVTALYAIPFISVLLVWTND 120 T+ E+ FWI VEY GMPFI P SL++IMYF+G+ERL++ + +Y +PFI+++LV TND Sbjct: 61 TLKEVKFWITVEYFGMPFIPPVSLMLIMYFLGMERLLKWQMRYLMYLMPFITLVLVMTND 120 Query: 121 YHHLFYQSMYFREGAPTPLIDVVMGPWYIVQGSLTFGCMLAGMCLILWQWNRMKRVYRRQ 180 HH +Y+S+ + G +++ GPWYI+QG TFGCM+ G+ L+L WNRM+ YR Q Sbjct: 121 MHHFYYRSVELQIGEGIHKVELNAGPWYIIQGGYTFGCMIGGVALLLIYWNRMRSSYRLQ 180 Query: 181 LFTIFIGQFLPALGAFLYLMDVTPYGMDPVPVIMSITSTLYIWAILSRGMLTAAPIAREN 240 T+ +G LP G FLYL ++TP G+DP+PVIM+IT+ LY+W ++S+GML AP+AR+ Sbjct: 181 HITMMVGLLLPIAGDFLYLGNLTPDGIDPIPVIMAITAALYMWGLVSKGMLNVAPVARDT 240 Query: 241 LFESMRDGVLVTDLSDMLVDYNRAAADMLQDLDASAIGK---PLAQLFLPAGQDAVDYVM 297 LFESM D VLV D ++ LVD+N AAA +L +L SA+G+ PL L +D Sbjct: 241 LFESMNDAVLVLDRNNRLVDFNPAAAAILPELTTSALGQAIEPLLALHTDHLMLDLDAEA 300 Query: 298 NSD-PLISEERELGWNMNGETRYYQIRSSPVQKYGGHQVGRMIMLIDVTERTLLQEKLLQ 356 D P I + E+ W + ++ YYQIRSS ++K G + ++I++ID+TER LQE+L Sbjct: 301 GGDMPHIYD--EVKWTVGMQSYYYQIRSSFIRKRRGPETSKLIVMIDITERVRLQEQLRV 358 Query: 357 LATVDSLTGIYNRMHFMELSRGLLNEAALSLAPVSIILLDIDFFKSINDRYGHHYGDMAL 416 LA D LTGI+NR+HF+ LS LL+++A P++++L DID FK IND YGH GD AL Sbjct: 359 LAYHDGLTGIFNRLHFIHLSEALLHQSAEQDEPLALLLFDIDHFKQINDAYGHDVGDRAL 418 Query: 417 QHIVTVCSSHLREGDIFGRYGGEEFVLCLPETSLKQA--ALLXXXXXXXXXXXXXXXXXG 474 H+V +C LR D+FGRYGGEEFV+ +P S ++A A Sbjct: 419 LHVVDICRQLLRPEDVFGRYGGEEFVVAMPGLSHEEAETAAQRIRSEIAAQTIAAATSEP 478 Query: 475 PIHVTASFGVVEA----RGKNLSLEDLLSEADHALYTSKRNGRNAV---HVSSGSS 523 P+ VTASFGV A + + LL AD+ALY +K +GRN V H+ G Sbjct: 479 PLSVTASFGVAMAGAGCKEAGGGINQLLKAADNALYEAKNSGRNTVRSAHIEPGKK 534 >Q2B6Y4.1 Q2B6Y4_9BACI Putative uncharacterized protein Length = 532 Score = 438 bits (1126), Expect = e-121, Method: Compositional matrix adjust. Identities = 233/516 (45%), Positives = 318/516 (61%), Gaps = 7/516 (1%) Query: 1 MGSMISNYIVIVSISGVLSALLALFAYYKKTDFAGTRAFILSSLTSAIYTFGFALELSGS 60 M S I +Y+V++S+SGVL+++L ++A+ K+ F+ + F+ ++TSAIY FG A EL+ Sbjct: 14 MNSDIFSYMVLISVSGVLTSILGMYAFAKRKTFSAGKTFVWIAVTSAIYIFGHAFELTAD 73 Query: 61 TMPEISFWIKVEYLGMPFIAPSSLLMIMYFVGLERLIRKGLVTALYAIPFISVLLVWTND 120 + EI FWI +Y G+PFI+P +L+M+M F GL+R + + LY IPFIS+++V TND Sbjct: 74 SRAEIKFWIAFQYAGLPFISPITLVMVMRFTGLDRYLTTFNLILLYTIPFISMVMVLTND 133 Query: 121 YHHLFYQSMYFREGAPTPLIDVVMGPWYIVQGSLTFGCMLAGMCLILWQWNRMKRVYRRQ 180 +H LFY L+D+ +G WY++ GS TFG + AG C+++W W R YRRQ Sbjct: 134 WHGLFYTGYLLHREGSYLLVDMDVGFWYVIHGSFTFGSLFAGACMVVWYWKRAGFAYRRQ 193 Query: 181 LFTIFIGQFLPALGAFLYLMDVTPYGMDPVPVIMSITSTLYIWAILSRGMLTAAPIAREN 240 L T+F G +P +FLYLM +PYGMDPVP++M +TS LY+ A LTAAPIA+E Sbjct: 194 LMTMFAGLTIPMTASFLYLMGYSPYGMDPVPMVMCLTSFLYLRAFF-MDRLTAAPIAKER 252 Query: 241 LFESMRDGVLVTDLSDMLVDYNRAAADMLQDLDASAIGKPLAQLFLPAGQDAVDYVMNSD 300 +FESMRDGVLV D LVD+N AA +ML LD+S+IG+ + + PA ++ Sbjct: 253 VFESMRDGVLVFAQDDGLVDFNPAAEEMLPGLDSSSIGEKMKVVLQPAAANSGTGADGGS 312 Query: 301 PLISEERELGWNMNGETRYYQIRSSPVQKYGGHQVGRMIMLIDVTERTLLQEKLLQLATV 360 + E N Y QI S P++K G G ++L ++T+ LL++KL +LA Sbjct: 313 YSGIHKFE-----NEAETYIQIHSFPIKKSSGELAGTTVILSNITQEVLLRQKLEELAFK 367 Query: 361 DSLTGIYNRMHFMELSRGLLNEAALSLAPVSIILLDIDFFKSINDRYGHHYGDMALQHIV 420 D LTGIYNR +F+E S LL EA + +S+IL DID+FK+IND+YGH GD AL+HI Sbjct: 368 DGLTGIYNRTYFIEQSGRLLKEAEAAGGTMSVILFDIDYFKNINDQYGHLQGDEALRHIA 427 Query: 421 TVCSSHLREGDIFGRYGGEEFVLCLPETSLKQAALLXXXXXXXXXXXXXXXXXGPIHVTA 480 V S L G IF RYGGEEFV+CLP L+ AA G I +TA Sbjct: 428 RVSGSLLGGGSIFARYGGEEFVICLPGADLESAADQAEKIRAGIEEVFLPSETGMISLTA 487 Query: 481 SFGVVEARGKNLSLEDLLSEADHALYTSKRNGRNAV 516 SFG EA ++LE LL AD ALY SK GRN V Sbjct: 488 SFGAAEA-APGMTLERLLHNADTALYQSKNGGRNRV 522 >B4AGE7.1 B4AGE7_BACPU Putative diguanylate cyclase Length = 529 Score = 384 bits (985), Expect = e-104, Method: Compositional matrix adjust. Identities = 205/525 (39%), Positives = 309/525 (58%), Gaps = 5/525 (0%) Query: 1 MGSMISNYIVIVSISGVLSALLALFAYYKKTDFAGTRAFILSSLTSAIYTFGFALELSGS 60 M S++ ++IVI+S+SG+LS +L++ A+ + + G + F+ S +AIY FG EL S Sbjct: 1 MDSIVLHFIVIMSVSGILSVILSIIAHANRHRYIGCQLFMYMSGATAIYIFGHTFELLAS 60 Query: 61 TMPEISFWIKVEYLGMPFIAPSSLLMIMYFVGLERLIRKGLVTALYAIPFISVLLVWTND 120 T EI FW+ +Y+G+PFIAP SL++++ F G + K V + IP ++ ++V+TN Sbjct: 61 TPREIMFWVCFQYIGLPFIAPFSLMIVLQFTGFHQYFTKRRVLLILLIPTLTTIMVFTNS 120 Query: 121 YHHLFYQSMYFREGAPTPLIDVVMGPWYIVQGSLTFGCMLAGMCLILWQWNRMKRVYRRQ 180 +H LFY+S+ L D +G WYI+ G TFGC + + +++W K+ Y +Q Sbjct: 121 FHSLFYRSITMNPVDGFLLADFTIGYWYIIHGVYTFGCSMLAIFMLVWFLRVTKKEYLKQ 180 Query: 181 LFTIFIGQFLPALGAFLYLMDVTPYGMDPVPVIMSITSTLYIWAILSRGMLTAAPIAREN 240 + + +G LP +F+YL+ VTP +DPVPV+M +T+ LY+WAI+S + +PIA E Sbjct: 181 ILILLVGMLLPITTSFIYLLGVTPLHIDPVPVVMFLTNALYLWAIMSFQLFKLSPIAMER 240 Query: 241 LFESMRDGVLVTDLSDMLVDYNRAAADMLQDL-DASAIGKPLAQLFLPAGQDAVDYVMNS 299 +FE M +GVL+ D L+DYN AAA ++ L + IG + +F +D + Sbjct: 241 VFEHMSEGVLILDRYRTLIDYNPAAASIIPALSNKKGIGYKIDDIFSGIIKDTAFFQWLR 300 Query: 300 DPLISEERELGWNM-NGETRYYQIRSSPVQKYGGHQVGRMIMLIDVTERTLLQEKLLQLA 358 P + +L W ++ YY + +S + G GR I++ DVTE+ LLQE+L + A Sbjct: 301 SPQDHSQYDLMWKKEEDQSSYYLLTASSLFNRRGEIEGRQIVISDVTEQRLLQEELERRA 360 Query: 359 TVDSLTGIYNRMHFMELSRGLLNEAALSLAPVSIILLDIDFFKSINDRYGHHYGDMALQH 418 +D LT I+NR F+E ++ +L E A A ++L DIDFFK IND +GHH GD AL+H Sbjct: 361 YIDGLTNIFNRSSFIERTQMILEEDAQDTA---VMLFDIDFFKQINDTHGHHIGDEALRH 417 Query: 419 IVTVCSSHLREGDIFGRYGGEEFVLCLPETSLKQAALLXXXXXXXXXXXXXXXXXGPIHV 478 +VT+C HL+ D+FGRYGGEEF +CLP S ++A + + V Sbjct: 418 VVTICQQHLQMDDLFGRYGGEEFAICLPHHSFEEACHIAEQIRHSFEMSFFENDEEKVRV 477 Query: 479 TASFGVVEARGKNLSLEDLLSEADHALYTSKRNGRNAVHVSSGSS 523 TASFG+ LE LL EAD ALY SKR GRN ++ S+G+ Sbjct: 478 TASFGIAHTVYDPNVLETLLFEADQALYMSKRQGRNWIYASTGAD 522 >A8FHG3.1 A8FHG3_BACP2 Possible diguanylate cyclase Length = 529 Score = 380 bits (977), Expect = e-103, Method: Compositional matrix adjust. Identities = 207/536 (38%), Positives = 311/536 (58%), Gaps = 27/536 (5%) Query: 1 MGSMISNYIVIVSISGVLSALLALFAYYKKTDFAGTRAFILSSLTSAIYTFGFALELSGS 60 M S++ ++IVI+S+SG+LS +L++ A+ + + G + F+ S +AIY FG EL S Sbjct: 1 MDSIVLHFIVIMSVSGILSVILSIIAHANRHRYIGCQLFMYMSGATAIYIFGHTFELLAS 60 Query: 61 TMPEISFWIKVEYLGMPFIAPSSLLMIMYFVGLERLIRKGLVTALYAIPFISVLLVWTND 120 T EI FW+ +Y+G+PFIAP SL++++ F G + K V + IP ++ ++V+TN Sbjct: 61 TPREIMFWVCFQYIGLPFIAPFSLMIVLQFTGFHQYFTKRRVLLILLIPTLTTIMVFTNS 120 Query: 121 YHHLFYQSMYFREGAPTPLIDVVMGPWYIVQGSLTFGCMLAGMCLILWQWNRMKRVYRRQ 180 +H LFY+S+ L D +G WYI+ G TFGC + + +++W K+ Y +Q Sbjct: 121 FHSLFYRSITMNPVDGFLLADFTIGYWYIIHGVYTFGCSMLAIFMLVWFLRVTKKEYLKQ 180 Query: 181 LFTIFIGQFLPALGAFLYLMDVTPYGMDPVPVIMSITSTLYIWAILSRGMLTAAPIAREN 240 + + +G LP +F+YL+ VTP +DPVPV+M +T+ LY+WAI+S + +PIA E Sbjct: 181 ILILLVGMLLPITTSFIYLLGVTPLHIDPVPVVMFLTNALYLWAIMSFQLFKLSPIAMER 240 Query: 241 LFESMRDGVLVTDLSDMLVDYNRAAADMLQDL-DASAIGKPLAQLF------------LP 287 +FE M +GVL+ D L+DYN AAA ++ L + IG + +F L Sbjct: 241 VFEHMSEGVLILDRYRTLIDYNPAAASIIPSLSNKKGIGFKIDDIFSGFIKDHDFIQWLR 300 Query: 288 AGQDAVDYVMNSDPLISEERELGWNMNGETRYYQIRSSPVQKYGGHQVGRMIMLIDVTER 347 + QD Y D ++ E++ + YY + +S + G GR I++ DVTE+ Sbjct: 301 SPQDHSQY----DLMLEREKD-------HSSYYHLTASSLFNRRGEVEGRQIVISDVTEQ 349 Query: 348 TLLQEKLLQLATVDSLTGIYNRMHFMELSRGLLNEAALSLAPVSIILLDIDFFKSINDRY 407 LLQE+L + A +D LT I+NR F+E ++ +L E A A ++L DIDFFK IND + Sbjct: 350 RLLQEELERRAYIDGLTNIFNRSSFIERTQMILEEDAQDTA---VMLFDIDFFKQINDTH 406 Query: 408 GHHYGDMALQHIVTVCSSHLREGDIFGRYGGEEFVLCLPETSLKQAALLXXXXXXXXXXX 467 GHH GD AL+H+VT+C HL+ D+FGRYGGEEF +CLP S ++A + Sbjct: 407 GHHIGDEALRHVVTICQQHLQMDDLFGRYGGEEFAICLPHHSFEEACHIAERIRHSFEMS 466 Query: 468 XXXXXXGPIHVTASFGVVEARGKNLSLEDLLSEADHALYTSKRNGRNAVHVSSGSS 523 + VTASFG+ LE LL EAD ALY SKR GRN ++ S+G+ Sbjct: 467 FFENEEEKVRVTASFGIAHTAYDPNVLETLLFEADQALYMSKRQGRNRIYASTGAD 522 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.139 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,953,088,400 Number of extensions: 120148955 Number of successful extensions: 395739 Number of sequences better than 1.0e-05: 13330 Number of HSP's gapped: 379590 Number of HSP's successfully gapped: 13351 Length of query: 530 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 389 Effective length of database: 1,732,333,088 Effective search space: 673877571232 Effective search space used: 673877571232 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)