BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0006.p (505 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q03657.1 Q03657_BACCI Amylase 847 0.0 B3K7Z9.1 B3K7Z9_9BACI Alpha amylase catalytic region 792 0.0 C1P738.1 C1P738_BACCO Alpha amylase catalytic region 719 0.0 B1YLA5.1 B1YLA5_EXIS2 Alpha amylase catalytic region 671 0.0 C4L3K1.1 C4L3K1_EXISA Alpha amylase catalytic region 670 0.0 >Q03657.1 Q03657_BACCI Amylase Length = 493 Score = 847 bits (2189), Expect = 0.0, Method: Compositional matrix adjust. Identities = 399/481 (82%), Positives = 438/481 (91%) Query: 1 MMQFFEWHVAADGQHWKRLAQMAPELKAAGIDSVWVPPVTKAISPEDTGYGVYDLYDLGE 60 MMQFFEWH+AADG HWKRLA+MAPELKA GID+VWVPPVTKA+S EDTGYGVYDLYDLGE Sbjct: 7 MMQFFEWHLAADGDHWKRLAEMAPELKAKGIDTVWVPPVTKAVSAEDTGYGVYDLYDLGE 66 Query: 61 FDQKGTIRTKYGTKQELIDAIAECLRNGIAVYVDLVMNHKAGADETEVFEVIEVDPNNRN 120 FDQKGT+RTKYGTKQELI+AIAEC +NGIAVYVDLVMNHKAGADETEVF+VIEVDPN+R Sbjct: 67 FDQKGTVRTKYGTKQELIEAIAECQKNGIAVYVDLVMNHKAGADETEVFKVIEVDPNDRT 126 Query: 121 KEISAPFEIEGWTKFTFPGRGDTYSSFKWNHTHFNGTDFDAKGGRNGVFRINGENKNWNQ 180 KEIS PFEIEGWTKFTFPGRGD YSSFKWN HFNGTDFDA+ R GVFRI GENK WN+ Sbjct: 127 KEISEPFEIEGWTKFTFPGRGDQYSSFKWNSEHFNGTDFDAREERTGVFRIAGENKKWNE 186 Query: 181 NVDDEFGNYDYLMFANIDYSQQEVRREMLEWGKWLVDTLQCSGYRLDAIKHINHEFIKEF 240 NVDDEFGNYDYLMFANIDY+ +VRREM++WGKWL+DTLQC G+RLDAIKHINHEFIKEF Sbjct: 187 NVDDEFGNYDYLMFANIDYNHPDVRREMIDWGKWLIDTLQCGGFRLDAIKHINHEFIKEF 246 Query: 241 AAEMTKKRGEDFYIVGEFWNSNLEACREFLNTVDYQIDLFDVSLHYKLYAAALGGRDFDL 300 AAEM +KRG+DFYIVGEFWNSNL+ACREFL+TVDYQIDLFDVSLHYKL+ A+L GRDFDL Sbjct: 247 AAEMIRKRGQDFYIVGEFWNSNLDACREFLDTVDYQIDLFDVSLHYKLHEASLKGRDFDL 306 Query: 301 THIFDDTLVQTHPGNAVTFVDNHDSQPHEALESWVGDWFKQSAYALILLRRDGYPVVFYG 360 + IFDDTLVQTHP +AVTFVDNHDSQPHEALESW+GDWFK SAYAL LLRRDGYPVVFYG Sbjct: 307 SKIFDDTLVQTHPTHAVTFVDNHDSQPHEALESWIGDWFKPSAYALTLLRRDGYPVVFYG 366 Query: 361 DYYGIGGPAPIEGKKAAIDPLLYARCHKAYGEQDDYFDHPNTIGWVRRGIEEIEGSGCAV 420 DYYGIGGP P++GKK +D LL ARC+KAYGEQ+DYFDH NTIGWVRRG+EEIEGSGCAV Sbjct: 367 DYYGIGGPEPVDGKKEILDILLSARCNKAYGEQEDYFDHANTIGWVRRGVEEIEGSGCAV 426 Query: 421 VISNGDNGEKRMFLGEERAGEVWVDFTHNRRESVTIGKDGWAVFPVNGGSVSVWALPDGA 480 VISNGD+GEKRMF+GE RAGEVWVD T + + +TI +DGWA F V GG VSVWALP+ Sbjct: 427 VISNGDDGEKRMFIGEHRAGEVWVDLTKSCDDQITIEEDGWATFHVCGGGVSVWALPEQN 486 Query: 481 E 481 E Sbjct: 487 E 487 >B3K7Z9.1 B3K7Z9_9BACI Alpha amylase catalytic region Length = 489 Score = 792 bits (2046), Expect = 0.0, Method: Compositional matrix adjust. Identities = 372/478 (77%), Positives = 415/478 (86%) Query: 1 MMQFFEWHVAADGQHWKRLAQMAPELKAAGIDSVWVPPVTKAISPEDTGYGVYDLYDLGE 60 +MQFFEWHVAADG HWKRL APEL AGID+VW+PPVTKA S ED GYGVYDLYDLGE Sbjct: 7 IMQFFEWHVAADGSHWKRLKNAAPELSKAGIDAVWIPPVTKASSAEDNGYGVYDLYDLGE 66 Query: 61 FDQKGTIRTKYGTKQELIDAIAECLRNGIAVYVDLVMNHKAGADETEVFEVIEVDPNNRN 120 FDQKGT+RTKYGTKQELIDAIA C +NGIAVYVDLVMNHKAGADETE F V++VD +R Sbjct: 67 FDQKGTVRTKYGTKQELIDAIAACQKNGIAVYVDLVMNHKAGADETERFRVVQVDEMDRT 126 Query: 121 KEISAPFEIEGWTKFTFPGRGDTYSSFKWNHTHFNGTDFDAKGGRNGVFRINGENKNWNQ 180 K+IS PFEIEGWTKFTFPGRGD YS+F+WN THFNG D+D R+G++RI GENK+W+ Sbjct: 127 KDISKPFEIEGWTKFTFPGRGDQYSAFQWNFTHFNGADYDEINKRSGIYRIVGENKSWSD 186 Query: 181 NVDDEFGNYDYLMFANIDYSQQEVRREMLEWGKWLVDTLQCSGYRLDAIKHINHEFIKEF 240 VD EFGNYDYLMFANIDYS +VR+EM+ WGKWLVDTLQCSG+RLDAIKHINH+FIKEF Sbjct: 187 KVDHEFGNYDYLMFANIDYSHPDVRKEMIAWGKWLVDTLQCSGFRLDAIKHINHDFIKEF 246 Query: 241 AAEMTKKRGEDFYIVGEFWNSNLEACREFLNTVDYQIDLFDVSLHYKLYAAALGGRDFDL 300 A M KKRG+DFYIVGEFWN L+ CR FL+TVDY+IDLFDVSLHYKL AA+ GR FDL Sbjct: 247 ATAMKKKRGDDFYIVGEFWNPELQDCRNFLDTVDYKIDLFDVSLHYKLQAASKAGRSFDL 306 Query: 301 THIFDDTLVQTHPGNAVTFVDNHDSQPHEALESWVGDWFKQSAYALILLRRDGYPVVFYG 360 IFDDTLVQ+HP NAVTFVDNHDSQP E+LESWV DWFKQSAYALILLR+DGYP VFYG Sbjct: 307 RTIFDDTLVQSHPMNAVTFVDNHDSQPQESLESWVEDWFKQSAYALILLRKDGYPSVFYG 366 Query: 361 DYYGIGGPAPIEGKKAAIDPLLYARCHKAYGEQDDYFDHPNTIGWVRRGIEEIEGSGCAV 420 DYYGIGG PI GK+ AIDPLLYAR ++AYGEQDDYFDHPNTIGWVRRGI EIE SGCAV Sbjct: 367 DYYGIGGENPIPGKREAIDPLLYARANRAYGEQDDYFDHPNTIGWVRRGIPEIECSGCAV 426 Query: 421 VISNGDNGEKRMFLGEERAGEVWVDFTHNRRESVTIGKDGWAVFPVNGGSVSVWALPD 478 +I+NG+ G+KRMF+GE RAGEVWVD T R++ VTIG+DG+ +FPVNGGSVSVWA PD Sbjct: 427 IIANGEAGDKRMFVGEHRAGEVWVDLTRTRKDRVTIGEDGFGLFPVNGGSVSVWAHPD 484 >C1P738.1 C1P738_BACCO Alpha amylase catalytic region Length = 487 Score = 719 bits (1856), Expect = 0.0, Method: Compositional matrix adjust. Identities = 337/475 (70%), Positives = 396/475 (83%) Query: 1 MMQFFEWHVAADGQHWKRLAQMAPELKAAGIDSVWVPPVTKAISPEDTGYGVYDLYDLGE 60 ++QFFEW+ ADG HW RL +MAPELK GID+VW+PPVTK S D GYGVYD YDLGE Sbjct: 7 ILQFFEWNTPADGSHWNRLKEMAPELKKTGIDAVWLPPVTKGQSDMDNGYGVYDHYDLGE 66 Query: 61 FDQKGTIRTKYGTKQELIDAIAECLRNGIAVYVDLVMNHKAGADETEVFEVIEVDPNNRN 120 FDQKGT+RTKYGTKQ+L +AI C + I VY+D+VMNHKAGADETE F+V+EVDP +RN Sbjct: 67 FDQKGTVRTKYGTKQQLHEAINACHEHDIQVYIDVVMNHKAGADETESFQVVEVDPMDRN 126 Query: 121 KEISAPFEIEGWTKFTFPGRGDTYSSFKWNHTHFNGTDFDAKGGRNGVFRINGENKNWNQ 180 KEIS PFEIEGWTKF F R YS F WNHTHF+G D+D + GRNG+FRI GENK+W++ Sbjct: 127 KEISEPFEIEGWTKFNFTNRKGKYSDFTWNHTHFSGVDYDNRTGRNGIFRIVGENKHWSE 186 Query: 181 NVDDEFGNYDYLMFANIDYSQQEVRREMLEWGKWLVDTLQCSGYRLDAIKHINHEFIKEF 240 +VD+EFGN+DYLM+A+IDY+ +V++EM+EWGKWL DT C GYRLDAIKHINH+FI++F Sbjct: 187 HVDNEFGNFDYLMYADIDYNHPDVKKEMIEWGKWLADTTGCDGYRLDAIKHINHDFIRDF 246 Query: 241 AAEMTKKRGEDFYIVGEFWNSNLEACREFLNTVDYQIDLFDVSLHYKLYAAALGGRDFDL 300 AA + + RG+ FY VGEFWN LEAC+++L+ V ++IDLFDV+LHYKL+ A+ GR FDL Sbjct: 247 AAALMEHRGDHFYFVGEFWNPQLEACQKYLDHVQFKIDLFDVALHYKLHEASKKGRAFDL 306 Query: 301 THIFDDTLVQTHPGNAVTFVDNHDSQPHEALESWVGDWFKQSAYALILLRRDGYPVVFYG 360 IF DTLVQTHP NAVTFVDNHDSQP+E+LESWV DWFKQSAYALILLR+DGYP VFYG Sbjct: 307 PTIFHDTLVQTHPLNAVTFVDNHDSQPNESLESWVDDWFKQSAYALILLRKDGYPCVFYG 366 Query: 361 DYYGIGGPAPIEGKKAAIDPLLYARCHKAYGEQDDYFDHPNTIGWVRRGIEEIEGSGCAV 420 D YGIGG PI GKK A+ PLL R KAYGEQDDYFDHPNTIGWVRRG+ E+ SGCAV Sbjct: 367 DMYGIGGDNPIPGKKDALSPLLSVRREKAYGEQDDYFDHPNTIGWVRRGVPEMPHSGCAV 426 Query: 421 VISNGDNGEKRMFLGEERAGEVWVDFTHNRRESVTIGKDGWAVFPVNGGSVSVWA 475 VISNG+NGEKRM +G+ERAGEVWVD T NR+E VTIG+DG+A FPVNGGSVSVW Sbjct: 427 VISNGENGEKRMLVGKERAGEVWVDATGNRQEKVTIGEDGYAGFPVNGGSVSVWV 481 >B1YLA5.1 B1YLA5_EXIS2 Alpha amylase catalytic region Length = 486 Score = 671 bits (1732), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/478 (65%), Positives = 375/478 (78%) Query: 1 MMQFFEWHVAADGQHWKRLAQMAPELKAAGIDSVWVPPVTKAISPEDTGYGVYDLYDLGE 60 MMQFFEWHV DG+HW+RL + APEL+AAGI SVW+PP +K S EDTGYG+YD+YDLGE Sbjct: 7 MMQFFEWHVENDGKHWQRLKERAPELRAAGITSVWIPPASKGQSDEDTGYGIYDVYDLGE 66 Query: 61 FDQKGTIRTKYGTKQELIDAIAECLRNGIAVYVDLVMNHKAGADETEVFEVIEVDPNNRN 120 FDQKGT+RTKYGTK ELI+AI N IAVY D+VMNHKA ADE E V+EV P +R+ Sbjct: 67 FDQKGTVRTKYGTKDELIEAIQVAHENDIAVYADVVMNHKAAADELETINVVEVHPEDRS 126 Query: 121 KEISAPFEIEGWTKFTFPGRGDTYSSFKWNHTHFNGTDFDAKGGRNGVFRINGENKNWNQ 180 KEIS FEIE WTKFTFPGR YS F W H FNGTDFDA+ + GVF+I+G+NK+WN Sbjct: 127 KEISEEFEIEAWTKFTFPGRNGKYSDFIWTHEFFNGTDFDAREEKTGVFKISGKNKDWND 186 Query: 181 NVDDEFGNYDYLMFANIDYSQQEVRREMLEWGKWLVDTLQCSGYRLDAIKHINHEFIKEF 240 VD+EFGNYDYLMFANIDY+ EVR EM+ WG W +T+ C G+RLDAIKHIN+EF+ EF Sbjct: 187 QVDNEFGNYDYLMFANIDYNHPEVREEMINWGNWFKETINCQGFRLDAIKHINYEFVNEF 246 Query: 241 AAEMTKKRGEDFYIVGEFWNSNLEACREFLNTVDYQIDLFDVSLHYKLYAAALGGRDFDL 300 A M + FY+VGEFW S+L+ CR+FL++VDY IDLFDV LHYK + A+ G+DFDL Sbjct: 247 AKSMIGDDPDGFYMVGEFWKSDLDDCRQFLDSVDYTIDLFDVPLHYKFHEASQQGQDFDL 306 Query: 301 THIFDDTLVQTHPGNAVTFVDNHDSQPHEALESWVGDWFKQSAYALILLRRDGYPVVFYG 360 T +F DTLV++HP NAVTFVDNHDSQP E+LESW+ DWFKQ AYA +LLR+DGYP VFYG Sbjct: 307 TTLFADTLVESHPTNAVTFVDNHDSQPGESLESWIDDWFKQHAYASVLLRKDGYPCVFYG 366 Query: 361 DYYGIGGPAPIEGKKAAIDPLLYARCHKAYGEQDDYFDHPNTIGWVRRGIEEIEGSGCAV 420 DYYG+ GP P+EGKK ID LLY R HKAYGEQ+DY D + +GWVRRG+EE SGCAV Sbjct: 367 DYYGVQGPHPVEGKKEMIDALLYTRYHKAYGEQEDYMDDAHCVGWVRRGVEEFPNSGCAV 426 Query: 421 VISNGDNGEKRMFLGEERAGEVWVDFTHNRRESVTIGKDGWAVFPVNGGSVSVWALPD 478 ++SN D EKRMF+GE RAGE W D+T+++ E V I ++G+ VFPV GG VSV+A D Sbjct: 427 LLSNADMCEKRMFVGETRAGEEWFDYTNHQEEPVVIDEEGFGVFPVPGGGVSVFAPRD 484 >C4L3K1.1 C4L3K1_EXISA Alpha amylase catalytic region Length = 490 Score = 670 bits (1729), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/480 (65%), Positives = 381/480 (79%), Gaps = 2/480 (0%) Query: 1 MMQFFEWHVAADGQHWKRLAQMAPELKAAGIDSVWVPPVTKAISPEDTGYGVYDLYDLGE 60 MMQFFEWHV AD +HWKRL + A +L AGI ++W+PPVTK S +DTGYG+YDLYDLGE Sbjct: 7 MMQFFEWHVEADQKHWKRLKERAADLNKAGITALWLPPVTKGQSDQDTGYGIYDLYDLGE 66 Query: 61 FDQKGTIRTKYGTKQELIDAIAECLRN-GIAVYVDLVMNHKAGADETEVFEVIEVDPNNR 119 FDQKG+I TKYGTK+EL++A C + G+ +Y+D+VMNHKAGAD TE F+V+EVDP++R Sbjct: 67 FDQKGSIATKYGTKKELLEATTYCQQELGMQIYMDVVMNHKAGADATEKFDVLEVDPDDR 126 Query: 120 NKEIS-APFEIEGWTKFTFPGRGDTYSSFKWNHTHFNGTDFDAKGGRNGVFRINGENKNW 178 K +S FEIEGWTKF + R YSSFKW+ THFNGTD+DA R+G+F I GENK W Sbjct: 127 TKVLSDEAFEIEGWTKFDYEARDGKYSSFKWDFTHFNGTDYDAATDRSGIFFIVGENKRW 186 Query: 179 NQNVDDEFGNYDYLMFANIDYSQQEVRREMLEWGKWLVDTLQCSGYRLDAIKHINHEFIK 238 N NVDDEFG YDYLMFANIDY +VR E+L WGKW ++ +C G+RLDAIKHIN++F+K Sbjct: 187 NDNVDDEFGVYDYLMFANIDYHHPDVREEVLNWGKWFIEETKCDGFRLDAIKHINYDFVK 246 Query: 239 EFAAEMTKKRGEDFYIVGEFWNSNLEACREFLNTVDYQIDLFDVSLHYKLYAAALGGRDF 298 EF+ M +++G+ FY VGEFW S+L+ACREFL+ +DY +DLFDV LHYKL+ A+ G F Sbjct: 247 EFSQTMREEKGDQFYFVGEFWKSDLDACREFLDNIDYHVDLFDVPLHYKLHQASEEGAAF 306 Query: 299 DLTHIFDDTLVQTHPGNAVTFVDNHDSQPHEALESWVGDWFKQSAYALILLRRDGYPVVF 358 D+ IFDDTLVQ+HP ++VTFVDNHDSQP EALESWV DWFKQ AY L+LLR+DGYP VF Sbjct: 307 DMRTIFDDTLVQSHPLHSVTFVDNHDSQPEEALESWVKDWFKQIAYGLVLLRKDGYPCVF 366 Query: 359 YGDYYGIGGPAPIEGKKAAIDPLLYARCHKAYGEQDDYFDHPNTIGWVRRGIEEIEGSGC 418 YGDYYGI GP P +G K AID LLYAR KAYGEQDDYFD N IGWVR+G+EEIE SGC Sbjct: 367 YGDYYGIDGPHPTDGMKLAIDTLLYARREKAYGEQDDYFDDANVIGWVRKGVEEIERSGC 426 Query: 419 AVVISNGDNGEKRMFLGEERAGEVWVDFTHNRRESVTIGKDGWAVFPVNGGSVSVWALPD 478 AV+IS D EKRMF+GEERAGE WVD T+ + + I ++G+ VF V+ GS+SVWALP+ Sbjct: 427 AVLISTSDEAEKRMFVGEERAGETWVDLTNTLDDRIEIDEEGFGVFKVSAGSISVWALPE 486 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,512,156,354 Number of extensions: 165250837 Number of successful extensions: 387801 Number of sequences better than 1.0e-05: 605 Number of HSP's gapped: 387273 Number of HSP's successfully gapped: 633 Length of query: 505 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 365 Effective length of database: 1,741,754,103 Effective search space: 635740247595 Effective search space used: 635740247595 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)