BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0176.0017.p (348 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBK2.1 B3KBK2_9BACI Metal dependent phophohydrolase 310 1e-82 B1DKG5.1 B1DKG5_9BACL Metal dependent phosphohydrolase 286 2e-75 C0ZH48.1 C0ZH48_BREBN Putative uncharacterized protein 179 3e-43 B4BPL4.1 B4BPL4_9BACI Metal dependent phosphohydrolase 177 9e-43 A4IJ90.1 A4IJ90_GEOTN Putative uncharacterized protein 177 1e-42 >B3KBK2.1 B3KBK2_9BACI Metal dependent phophohydrolase Length = 348 Score = 310 bits (794), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 152/348 (43%), Positives = 224/348 (64%) Query: 1 VRIHIKKLKIGDVLKTDIFNRQGLHVLAKKTKIRLEDIALLNKHSIKFVDVEGSVEDRIA 60 +R+H+ LK GD ++ D +N G+HVL K +R EDI+ L +H I +++++ Sbjct: 1 MRVHVTDLKPGDQIQQDAYNTNGVHVLQKGALLRSEDISKLLQHGIDYIEIQPRALSVSL 60 Query: 61 REVFNGTERPLRVKPNYDKSLREYQAFFLEAIISGEIDRSKIEWTLMPLLEQLDEQKDVL 120 + ++ +VKP+++ + A F EA SG+ + S ++ PL+ L+E DV+ Sbjct: 61 DPLSEISDSFNKVKPHFNLAFDGCSALFTEASQSGKFNESVVDDVFQPLVGSLNEHTDVV 120 Query: 121 TLLFSFQKEDETIYNHSLQVGLLSYYIAEWLGYPKSERFEICRAGYLHDIGKSRVRLSIR 180 +LL F D+ Y HS+QVG+LSYYIA+W+GY E +EI +AGYLHDIGKS++ I Sbjct: 121 SLLLLFNGTDDYTYRHSIQVGMLSYYIADWMGYSGKEAYEIGKAGYLHDIGKSKISRDIL 180 Query: 181 NRVGHLNDEEEKELQKHTSAGYQIIRNSLKDDTAALVALQHHEREDGTGYPLGVHKEKIH 240 ++ G L EE +E++ HT GY++I S+ D A+VALQHHER+D +GYP +H ++IH Sbjct: 181 DKPGKLTPEEFEEVKLHTIYGYEMIAESMDDKVTAMVALQHHERDDRSGYPRALHADQIH 240 Query: 241 PYAQIVAVANEFISKTFVSAEQPKRNFSTVLQEMYELGFGKLNEKPVQALIHNLLPNMIG 300 PY+ I AVA+ + + T Q K+ TVL+E+Y L FGKLN KP A I +LPN IG Sbjct: 241 PYSHICAVADVYSAMTTNRVYQSKQQLLTVLRELYSLSFGKLNGKPTHAFIQQMLPNFIG 300 Query: 301 KKVQLSNGHIGEIILNNPMDFFRPLVKVKETFIDLSKERGLDIREIIV 348 KKV L+ G G I++N+P D+FRPLVK + FIDLSKE +I+EI++ Sbjct: 301 KKVLLTTGESGIIVMNHPSDYFRPLVKTDDRFIDLSKEYHTEIQEILL 348 >B1DKG5.1 B1DKG5_9BACL Metal dependent phosphohydrolase Length = 354 Score = 286 bits (733), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 145/354 (40%), Positives = 223/354 (62%), Gaps = 8/354 (2%) Query: 1 VRIHIKKLKIGDVLKTDIFNRQGLHVLAKKTKIRLEDIALLNKHSIKFVDVEG--SVEDR 58 +R+HI LK GD+L D FN GLHVL+K T + +DIA L +H + +VD+E + E Sbjct: 1 MRLHISDLKEGDILLKDTFNGYGLHVLSKGTVLAQKDIAKLFQHQVDYVDIEDRQTTEQP 60 Query: 59 IAREVFNGTERP---LRVKPNYDKSLREYQAFFLEAIISGEIDRSKIEWTLMPLLEQLDE 115 I R T P V+P+Y+ +++ +Q F +A G + + ++ PLL+ Sbjct: 61 IHR-TLETTVNPKWLPNVRPDYENAVKGFQGLFQKATDGGVVKQDEVAAAFQPLLKNFQM 119 Query: 116 QKDVLTLLFSFQKEDETIYNHSLQVGLLSYYIAEWLGYPKSERFEICRAGYLHDIGKSRV 175 ++DV+++L D+ Y HS+QVG+LSYY+A WLGY + E EI ++GYLHDIGKSR+ Sbjct: 120 ERDVVSMLLLLNTADDYTYQHSVQVGMLSYYLASWLGYTEMEALEISKSGYLHDIGKSRI 179 Query: 176 RLSIRNRVGHLNDEEEKELQKHTSAGYQIIRNSLKDDT--AALVALQHHEREDGTGYPLG 233 SI N+ L DEE +E++KHT GY II+ S +D + A+ ALQHHER DG+GYP G Sbjct: 180 PSSILNKPAKLTDEEFEEVKKHTLYGYDIIQQSFEDKSEIMAVSALQHHERNDGSGYPHG 239 Query: 234 VHKEKIHPYAQIVAVANEFISKTFVSAEQPKRNFSTVLQEMYELGFGKLNEKPVQALIHN 293 + ++IHP A+++AV + + + + KR+ VL+E+Y++ F +L+ I + Sbjct: 240 LRGDEIHPVAKLIAVVDIYSAMISERVYKKKRDLFFVLKELYKMSFNELDAHTTHTFIKH 299 Query: 294 LLPNMIGKKVQLSNGHIGEIILNNPMDFFRPLVKVKETFIDLSKERGLDIREII 347 ++PN IGKK L +G +G I++ +P +FFRPL++ E FIDL+ +R +I+EII Sbjct: 300 MIPNFIGKKAVLESGEVGTIVMTHPTEFFRPLIQTGERFIDLTVDREYEIKEII 353 >C0ZH48.1 C0ZH48_BREBN Putative uncharacterized protein Length = 360 Score = 179 bits (455), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 110/358 (30%), Positives = 190/358 (53%), Gaps = 13/358 (3%) Query: 3 IHIKKLKIGDVLKTDIFNRQGLHVLAKKTKIRLEDIALLNKHSIKFVDVEGSVEDRIAR- 61 + +K+L +G L D+F G + AK T + ++ L IK V VE S D + Sbjct: 1 MEVKQLTLGAKLAEDVFTALGGILFAKGTPLYEREVQFLEAFMIKQVQVEDSGGDLTDQG 60 Query: 62 -------EVFNGTERPLRVKP----NYDKSLREYQAFFLEAIISGEIDRSKIEWTLMPLL 110 + +++ + KP ++DK++ + I ++ + P++ Sbjct: 61 EPTSTKVDAPEASDKFTQAKPVFQESFDKAVSTLKNLMTRVQGGNNIPVMEVREVVTPII 120 Query: 111 EQLDEQKDVLTLLFSFQKEDETIYNHSLQVGLLSYYIAEWLGYPKSERFEICRAGYLHDI 170 + +Q VL L F K D Y H++ VG++SY IA+W+ P+ E ++ AG L DI Sbjct: 121 TEFQQQPQVLLSLRRFAKMDSYAYEHAVAVGIISYMIAKWVKVPEKEWMQVALAGTLLDI 180 Query: 171 GKSRVRLSIRNRVGHLNDEEEKELQKHTSAGYQIIRNSLK-DDTAALVALQHHEREDGTG 229 GK+++ I + G L +E +E++KHT GYQII++S + AL ALQHHEREDG+G Sbjct: 181 GKTKIDRRILQKPGKLTPDEFEEMKKHTVYGYQIIKSSHGLSEGVALAALQHHEREDGSG 240 Query: 230 YPLGVHKEKIHPYAQIVAVANEFISKTFVSAEQPKRNFSTVLQEMYELGFGKLNEKPVQA 289 YPLG+ K+H Y++IVAVA+ + + + Q + V++++ + FGKL+ V++ Sbjct: 241 YPLGLPGSKLHLYSKIVAVADVYHAMSSDRVHQKALSPYQVVEQLVQDSFGKLDPTIVRS 300 Query: 290 LIHNLLPNMIGKKVQLSNGHIGEIILNNPMDFFRPLVKVKETFIDLSKERGLDIREII 347 + + +G V+LS+G IG+I+ + RP+V+ ++L + R L I ++ Sbjct: 301 FVEGITQFAVGTLVELSDGTIGKIVFTDRNHPTRPMVETGGKIVNLVEARHLSIVRVM 358 >B4BPL4.1 B4BPL4_9BACI Metal dependent phosphohydrolase Length = 353 Score = 177 bits (450), Expect = 9e-43, Method: Compositional matrix adjust. Identities = 115/359 (32%), Positives = 200/359 (55%), Gaps = 19/359 (5%) Query: 1 VRIHIKKLKIGDVLKTDIFNRQGLHVLAKKTKIRLEDIALLNKHSIKFVDVE---GSVED 57 VR+ +++L+ G VL D+ + ++ KKT I + + +L+K I V++E + E Sbjct: 2 VRVKLEQLREGCVLAQDVIGKTKKPIMPKKTVITKQLLHVLSKFLINEVEIEPVLANGEP 61 Query: 58 RIAREVFN--GTERPLRVKPNYDKSLREYQAFFLEAIISGEIDRSKIEWTLMPLLEQLDE 115 +A + + ERP+ + Y ++++ Y+ F G +D +I +PL ++ + Sbjct: 62 FLAGDSASTKADERPVML---YLEAVQRYKKAFQSWQAGGAVDIGEIREIAIPLFNKMIK 118 Query: 116 QKDVLTLLFSFQKEDETIYNHSLQVGLLSYYIAEWLGYPKSERFEICRAGYLHDIGKSRV 175 K L +L + ++E +Y+H++ VGLL+ ++A LGY + E +I AG L D G +++ Sbjct: 119 HKRELLMLHHYTTKEEYLYHHAVSVGLLAGFLAMQLGYSQGECHQIVLAGVLSDCGMAKI 178 Query: 176 RLSIRNRVGHLNDEEEKELQKHTSAGYQIIRN-SLKDDTAALVALQHHEREDGTGYPLGV 234 I + L +EE +E+++H Y++++N + + A L LQHHER DG+GYPL V Sbjct: 179 SSQILMKPSALTEEEFREIEQHPILSYRMVQNIAALNQGAKLAVLQHHERNDGSGYPLRV 238 Query: 235 HKEKIHPYAQIVAVANEFISKT----FVSAEQPKRNFSTVLQEMYELGFGKLNEKPVQAL 290 EKIHPY Q++AVA+ + + T + + P R + +E FG+LN + V L Sbjct: 239 KAEKIHPYGQLIAVADVYYAMTSERLYRRKQSPFRAIEAIQREQ----FGRLNAEVVALL 294 Query: 291 IHNLLPNMIGKKVQLSNGHIGEIILNNPMDFFRPLVKVKET--FIDLSKERGLDIREII 347 +++L+ G V+LSNG E+I P + RP+VKV +T + L ++R L I EI+ Sbjct: 295 VNHLISLQTGAIVKLSNGETAEVIFIQPDEPARPIVKVMKTGQLLSLVEQRDLYIEEIL 353 >A4IJ90.1 A4IJ90_GEOTN Putative uncharacterized protein Length = 353 Score = 177 bits (450), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 115/359 (32%), Positives = 200/359 (55%), Gaps = 19/359 (5%) Query: 1 VRIHIKKLKIGDVLKTDIFNRQGLHVLAKKTKIRLEDIALLNKHSIKFVDVE---GSVED 57 VR+ +++L+ G VL D+ + ++ KKT I + + +L+K I V++E + E Sbjct: 2 VRVKLEQLREGCVLAQDVIGKTKKPIMPKKTVITKQLLHVLSKFLINEVEIEPVLANGEP 61 Query: 58 RIAREVFN--GTERPLRVKPNYDKSLREYQAFFLEAIISGEIDRSKIEWTLMPLLEQLDE 115 +A + + ERP+ + Y ++++ Y+ F G +D +I +PL ++ + Sbjct: 62 FLAGDSASTKADERPVML---YLQAVQRYKKAFQSWQAGGAVDIVEIREIAIPLFNKMIK 118 Query: 116 QKDVLTLLFSFQKEDETIYNHSLQVGLLSYYIAEWLGYPKSERFEICRAGYLHDIGKSRV 175 K L +L + ++E +Y+H++ VGLL+ ++A LGY + E +I AG L D G +++ Sbjct: 119 HKRELLMLHHYTTKEEYLYHHAVSVGLLAGFLAMQLGYGQGECHQIVLAGVLSDCGMAKI 178 Query: 176 RLSIRNRVGHLNDEEEKELQKHTSAGYQIIRN-SLKDDTAALVALQHHEREDGTGYPLGV 234 I + L + E +E+++H GY++++N + + A L LQHHER DG+GYPL V Sbjct: 179 SSQILMKPSALTEAEFREIEQHPILGYRMVQNIAALNQGAKLAVLQHHERNDGSGYPLRV 238 Query: 235 HKEKIHPYAQIVAVANEFISKT----FVSAEQPKRNFSTVLQEMYELGFGKLNEKPVQAL 290 EKIHPY Q++AVA+ + + T + + P R + +E FG+LN + V L Sbjct: 239 KAEKIHPYGQLIAVADVYYAMTSERLYRRKQSPFRAIEAIQREQ----FGRLNAEVVALL 294 Query: 291 IHNLLPNMIGKKVQLSNGHIGEIILNNPMDFFRPLVKVKET--FIDLSKERGLDIREII 347 +++L+ G V+LSNG E+I P + RP+VKV +T + L ++R L I EI+ Sbjct: 295 VNHLISLQTGAIVKLSNGETAEVIFIQPDEPARPIVKVMKTGQLLSLVEQRDLYIEEIL 353 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.139 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,032,669,903 Number of extensions: 85350440 Number of successful extensions: 212217 Number of sequences better than 1.0e-05: 1967 Number of HSP's gapped: 211004 Number of HSP's successfully gapped: 1976 Length of query: 348 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 212 Effective length of database: 1,779,438,163 Effective search space: 377240890556 Effective search space used: 377240890556 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)