BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0176.0010.p (463 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL09.1 B3KL09_9BACI Glutamyl-tRNA reductase EC=1.2.1.70 707 0.0 O69108.1 HEM1_PAEMA Glutamyl-tRNA reductase EC=1.2.1.70 665 0.0 B1DDT3.1 B1DDT3_9BACL Glutamyl-tRNA reductase EC=1.2.1.70 593 e-168 Q9K8F8.1 HEM1_BACHD Glutamyl-tRNA reductase EC=1.2.1.70 498 e-139 A6CN67.1 A6CN67_9BACI Glutamyl-tRNA reductase EC=1.2.1.70 498 e-139 >B3KL09.1 B3KL09_9BACI Glutamyl-tRNA reductase EC=1.2.1.70 Length = 468 Score = 707 bits (1826), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/420 (79%), Positives = 374/420 (89%) Query: 1 MHIVVVGLNYRTAPVEVREQFAFAEKDLPAALHQLMQTKSVLEGVVVATCNRTEIYVVVD 60 MHIVVVGLNYRTAPVEVRE+F F+E+D+P AL QL TKSVLEGV+VATCNRTEIYVVVD Sbjct: 1 MHIVVVGLNYRTAPVEVRERFTFSEEDMPKALEQLNSTKSVLEGVIVATCNRTEIYVVVD 60 Query: 61 RLHMCGYFIRSYMEQWFGVKSDVFAQHMYIYEDEQAIAHLFRVTCGLDSMVIGETQILGQ 120 RLHMCGYFIRS+MEQWFG+ + F QH+YIYEDEQAI+HLF+VTCGLDSMVIGETQILGQ Sbjct: 61 RLHMCGYFIRSFMEQWFGINREEFTQHLYIYEDEQAISHLFKVTCGLDSMVIGETQILGQ 120 Query: 121 VRSAFLTAQAEGVTGTWFNRLFKQAVTLGKRAHSETSIGESAVSVSYAAVELGKRIFGMF 180 VR+AFLT+Q TGTWFN LFKQAVTLGKRAHSETSIGESAVSVSYAAVELGKRIFG F Sbjct: 121 VRNAFLTSQKHDATGTWFNMLFKQAVTLGKRAHSETSIGESAVSVSYAAVELGKRIFGAF 180 Query: 181 TGKRVLILGAGKMSELTVKHLYSSGAAEVIVANRTLARAAELAGKFSGKPSTIEHALQHL 240 K+V+ILGAGKMSELTVKHLY++GAAEVIVANRTL RA ELA KF G P TI A L Sbjct: 181 NDKKVMILGAGKMSELTVKHLYANGAAEVIVANRTLGRAEELARKFKGTPCTIAEAKNLL 240 Query: 241 DEVDIVISSTGADGYVLTADQVADNMKRRPSRPLFMIDIAVPRDIDPAAASLPDVFLYDI 300 ++VDIVISSTG++ YVL A++V ++MK+R SRPLFMIDIAVPRDIDPA L +VFLYDI Sbjct: 241 NKVDIVISSTGSNEYVLNAERVKESMKKRQSRPLFMIDIAVPRDIDPAVGELSNVFLYDI 300 Query: 301 DDLEGIVETNLEMRRSEAAKIEVMIEAELEDFQMWLKTLGVRPVIRALQDKSNGIYEDTM 360 DDLEGIVETNLEMRR EAAKIEVMI+ E++ F WLKTLGVRP IRALQ+KSN I+E+TM Sbjct: 301 DDLEGIVETNLEMRRVEAAKIEVMIQEEMDAFHTWLKTLGVRPAIRALQEKSNAIHEETM 360 Query: 361 DSLFNKLPELDEHQRKVIRRLTKSIVNQMMHDPINVIKELSGGKQGNEALEYFTQIFALQ 420 +SLFNKLPELDE QRKVIRRLTKSIVNQMMHDPIN IKE++GGKQG EAL+YF+QIFAL+ Sbjct: 361 ESLFNKLPELDERQRKVIRRLTKSIVNQMMHDPINRIKEMTGGKQGGEALDYFSQIFALE 420 >O69108.1 HEM1_PAEMA Glutamyl-tRNA reductase EC=1.2.1.70 Length = 437 Score = 665 bits (1717), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/423 (74%), Positives = 366/423 (86%) Query: 1 MHIVVVGLNYRTAPVEVREQFAFAEKDLPAALHQLMQTKSVLEGVVVATCNRTEIYVVVD 60 MHIVVVGLNYRTAPVEVRE+F+FAEKD+P AL +L++TKSVLEGV++ATCNRTEIYVVVD Sbjct: 1 MHIVVVGLNYRTAPVEVRERFSFAEKDMPQALQELLRTKSVLEGVIIATCNRTEIYVVVD 60 Query: 61 RLHMCGYFIRSYMEQWFGVKSDVFAQHMYIYEDEQAIAHLFRVTCGLDSMVIGETQILGQ 120 RLHMCGYFIRS+ME+WFGV + F +H+Y+YEDEQA+ HLFRV CGLDSMV+GETQILGQ Sbjct: 61 RLHMCGYFIRSFMERWFGVPREEFTRHLYMYEDEQAVRHLFRVACGLDSMVLGETQILGQ 120 Query: 121 VRSAFLTAQAEGVTGTWFNRLFKQAVTLGKRAHSETSIGESAVSVSYAAVELGKRIFGMF 180 V+SAFL +Q + TGTWFN LFKQAVTLGKRAHSET+IG+SAVS+SYAAVELGKRIFG F Sbjct: 121 VKSAFLLSQRQKGTGTWFNMLFKQAVTLGKRAHSETAIGQSAVSISYAAVELGKRIFGSF 180 Query: 181 TGKRVLILGAGKMSELTVKHLYSSGAAEVIVANRTLARAAELAGKFSGKPSTIEHALQHL 240 +GKRVLILGAGKMSELT KHL GA EVIVANRT ARA ELA KF G P T++ A++ L Sbjct: 181 SGKRVLILGAGKMSELTAKHLSGGGADEVIVANRTYARAQELAAKFDGTPCTMQEAMERL 240 Query: 241 DEVDIVISSTGADGYVLTADQVADNMKRRPSRPLFMIDIAVPRDIDPAAASLPDVFLYDI 300 +VDI+ISSTGA+G QV +MKRRP RPLFMIDIAVPRDI+P L +VFLYDI Sbjct: 241 ADVDILISSTGAEGIRDHVGQVERSMKRRPDRPLFMIDIAVPRDIEPEIGVLENVFLYDI 300 Query: 301 DDLEGIVETNLEMRRSEAAKIEVMIEAELEDFQMWLKTLGVRPVIRALQDKSNGIYEDTM 360 DDLEGIVE NLEMRR+EA KI+ MIE E++ F WL+TLGV+PVIRALQ+K+ I+E T+ Sbjct: 301 DDLEGIVENNLEMRRAEAVKIDKMIEEEMQVFANWLQTLGVKPVIRALQEKAAHIHESTL 360 Query: 361 DSLFNKLPELDEHQRKVIRRLTKSIVNQMMHDPINVIKELSGGKQGNEALEYFTQIFALQ 420 DS+FNKLPELDE QRKVIRRLTKSI+NQMMHDPIN IKE++GGKQG EALE FTQIFAL+ Sbjct: 361 DSMFNKLPELDERQRKVIRRLTKSILNQMMHDPINRIKEMAGGKQGAEALEMFTQIFALE 420 Query: 421 EQL 423 + L Sbjct: 421 KHL 423 >B1DDT3.1 B1DDT3_9BACL Glutamyl-tRNA reductase EC=1.2.1.70 Length = 456 Score = 593 bits (1530), Expect = e-168, Method: Compositional matrix adjust. Identities = 282/424 (66%), Positives = 341/424 (80%) Query: 1 MHIVVVGLNYRTAPVEVREQFAFAEKDLPAALHQLMQTKSVLEGVVVATCNRTEIYVVVD 60 MHI+VVGLNYRTAPVEVRE+FA AEK+LP A+ QLM T S++E V+VATCNRTE+Y VVD Sbjct: 1 MHIIVVGLNYRTAPVEVRERFAIAEKELPEAMKQLMNTSSIMECVIVATCNRTELYAVVD 60 Query: 61 RLHMCGYFIRSYMEQWFGVKSDVFAQHMYIYEDEQAIAHLFRVTCGLDSMVIGETQILGQ 120 R H+CG++IRS+MEQWF + + F H+Y++ED++AI HLFRVT GLDSMVIGETQILGQ Sbjct: 61 RHHLCGHYIRSFMEQWFKLPREQFTSHLYMHEDDKAIEHLFRVTSGLDSMVIGETQILGQ 120 Query: 121 VRSAFLTAQAEGVTGTWFNRLFKQAVTLGKRAHSETSIGESAVSVSYAAVELGKRIFGMF 180 V+ AF Q + TGT FN LFKQAVT+ KRAHSET+IGESAVSVSYAAVELGKRIFG F Sbjct: 121 VKDAFALGQQQKTTGTLFNMLFKQAVTMAKRAHSETTIGESAVSVSYAAVELGKRIFGQF 180 Query: 181 TGKRVLILGAGKMSELTVKHLYSSGAAEVIVANRTLARAAELAGKFSGKPSTIEHALQHL 240 + K V+++GAG+ ELT KHLY++GA VIV NRT RAA+LA KF+G +E A L Sbjct: 181 SKKTVMVIGAGETGELTAKHLYANGADRVIVVNRTYERAAQLAEKFNGIACPMEDAASKL 240 Query: 241 DEVDIVISSTGADGYVLTADQVADNMKRRPSRPLFMIDIAVPRDIDPAAASLPDVFLYDI 300 E DIVISSTGADGYVL VA+ MKRR S+PLFMIDIAVPRD+DP A++ +VFLYDI Sbjct: 241 HEADIVISSTGADGYVLERQAVAEAMKRRKSKPLFMIDIAVPRDLDPGIAAVENVFLYDI 300 Query: 301 DDLEGIVETNLEMRRSEAAKIEVMIEAELEDFQMWLKTLGVRPVIRALQDKSNGIYEDTM 360 DDLEGIV +NLE RR EA KIE MIE E E F+ W KTLGV P+IRALQ K+ I+E+T+ Sbjct: 301 DDLEGIVASNLEQRRKEAEKIETMIEQESEQFEQWYKTLGVVPLIRALQTKAAHIHEETL 360 Query: 361 DSLFNKLPELDEHQRKVIRRLTKSIVNQMMHDPINVIKELSGGKQGNEALEYFTQIFALQ 420 +SL NKLP+LDEH+ KVIR+LTKSIVNQMM DPI IKE++G K EA++ F ++FAL+ Sbjct: 361 NSLTNKLPDLDEHELKVIRKLTKSIVNQMMQDPILRIKEMAGEKHSEEAMDMFVKLFALE 420 Query: 421 EQLG 424 ++L Sbjct: 421 DELA 424 >Q9K8F8.1 HEM1_BACHD Glutamyl-tRNA reductase EC=1.2.1.70 Length = 459 Score = 498 bits (1282), Expect = e-139, Method: Compositional matrix adjust. Identities = 247/417 (59%), Positives = 320/417 (76%) Query: 1 MHIVVVGLNYRTAPVEVREQFAFAEKDLPAALHQLMQTKSVLEGVVVATCNRTEIYVVVD 60 MHI+++GLNY+TAPVE+RE+F+F + +LP ALHQL Q KS+LE +V+TCNRTE+YVV D Sbjct: 1 MHILMIGLNYKTAPVEIREKFSFQDSELPQALHQLRQMKSILECTIVSTCNRTELYVVAD 60 Query: 61 RLHMCGYFIRSYMEQWFGVKSDVFAQHMYIYEDEQAIAHLFRVTCGLDSMVIGETQILGQ 120 +LH +F ++++ WF + D F + I E++ AI HLFRV GLDSM++GETQILGQ Sbjct: 61 QLHTGRHFTKTFLADWFKLPKDEFTPFLTIRENDHAIEHLFRVVTGLDSMILGETQILGQ 120 Query: 121 VRSAFLTAQAEGVTGTWFNRLFKQAVTLGKRAHSETSIGESAVSVSYAAVELGKRIFGMF 180 VR++F AQ E VTG+ FN LFKQA+TL KRAHSET IG++AVSVSYAAVELGK+IF F Sbjct: 121 VRNSFFIAQEEQVTGSIFNHLFKQAITLAKRAHSETDIGQNAVSVSYAAVELGKKIFDDF 180 Query: 181 TGKRVLILGAGKMSELTVKHLYSSGAAEVIVANRTLARAAELAGKFSGKPSTIEHALQHL 240 GK+VLILGAGKM ELT KHL+S+GA +V V NRT +AAELA +F G + + Sbjct: 181 KGKQVLILGAGKMGELTAKHLHSNGAEQVTVINRTREKAAELAKRFLGVDRPYNELTEAI 240 Query: 241 DEVDIVISSTGADGYVLTADQVADNMKRRPSRPLFMIDIAVPRDIDPAAASLPDVFLYDI 300 E DI+ISSTGA GYV+T+D V+ +K+R RPLFM+DIAVPRD+DPA AS DV+LYDI Sbjct: 241 VEADILISSTGATGYVVTSDMVSHALKKRKGRPLFMVDIAVPRDLDPALASHDDVYLYDI 300 Query: 301 DDLEGIVETNLEMRRSEAAKIEVMIEAELEDFQMWLKTLGVRPVIRALQDKSNGIYEDTM 360 DDL+ IV+TNLE RR+EA KIE++IE EL +F+ WL TLGV P+I AL+ K+ + +TM Sbjct: 301 DDLQNIVQTNLEERRTEAEKIELLIEEELVEFKQWLNTLGVVPIITALRTKALTVQGETM 360 Query: 361 DSLFNKLPELDEHQRKVIRRLTKSIVNQMMHDPINVIKELSGGKQGNEALEYFTQIF 417 +S+ KLP L E ++KV+R+ TKSIVNQ++ DPI IKEL+ + EAL+ FT+IF Sbjct: 361 ESIERKLPNLTEREKKVLRKHTKSIVNQLLRDPITRIKELANAPEREEALDLFTKIF 417 >A6CN67.1 A6CN67_9BACI Glutamyl-tRNA reductase EC=1.2.1.70 Length = 451 Score = 498 bits (1281), Expect = e-139, Method: Compositional matrix adjust. Identities = 237/429 (55%), Positives = 312/429 (72%) Query: 1 MHIVVVGLNYRTAPVEVREQFAFAEKDLPAALHQLMQTKSVLEGVVVATCNRTEIYVVVD 60 MHI+ VG+NY+TAPVE+RE+ +F E DL A+ L KS+LE V+V+TCNRTE+Y VVD Sbjct: 1 MHIITVGINYKTAPVEIRERLSFNEADLQRAMETLKGKKSILENVIVSTCNRTEVYAVVD 60 Query: 61 RLHMCGYFIRSYMEQWFGVKSDVFAQHMYIYEDEQAIAHLFRVTCGLDSMVIGETQILGQ 120 +LH Y+I+ ++ QWF + + F +++IYE + A+ HLF VTCGL+SMV+GETQILGQ Sbjct: 61 QLHTGRYYIKEFLSQWFDIDKEEFTPYLFIYEQDGAVEHLFNVTCGLNSMVVGETQILGQ 120 Query: 121 VRSAFLTAQAEGVTGTWFNRLFKQAVTLGKRAHSETSIGESAVSVSYAAVELGKRIFGMF 180 +RS+FL+AQ G TGT FN LFKQA+TL K+AHSET IG +AVSVSYAAVEL K+IFG Sbjct: 121 IRSSFLSAQESGTTGTVFNHLFKQAITLAKKAHSETEIGSNAVSVSYAAVELAKKIFGDL 180 Query: 181 TGKRVLILGAGKMSELTVKHLYSSGAAEVIVANRTLARAAELAGKFSGKPSTIEHALQHL 240 +GK VLI GAGKM EL +K+L+ SGA +V V NRT +A LA KF G T++ L Sbjct: 181 SGKHVLIFGAGKMGELAIKNLHGSGATKVTVINRTFEKAVSLADKFKGNAKTLQELQCAL 240 Query: 241 DEVDIVISSTGADGYVLTADQVADNMKRRPSRPLFMIDIAVPRDIDPAAASLPDVFLYDI 300 E DI+ISSTGA YV+T DQ+ + + R RPLFM+DIAVPRDIDPA SL FLYDI Sbjct: 241 VEADILISSTGAKEYVITKDQMVEVERMRKGRPLFMVDIAVPRDIDPAIESLESAFLYDI 300 Query: 301 DDLEGIVETNLEMRRSEAAKIEVMIEAELEDFQMWLKTLGVRPVIRALQDKSNGIYEDTM 360 DDLEGIV++N+ R+ A I +MIEAE+ F+ WL LGV PVI AL++K+ I TM Sbjct: 301 DDLEGIVQSNMAERQKAAESIGIMIEAEIVAFKEWLNMLGVVPVISALREKALVIQGQTM 360 Query: 361 DSLFNKLPELDEHQRKVIRRLTKSIVNQMMHDPINVIKELSGGKQGNEALEYFTQIFALQ 420 +S+ K+P+L E +RKV+ + TKSI+NQ++ DPI +KEL+ G E L+ F +IF ++ Sbjct: 361 ESIERKMPDLTERERKVLNKHTKSIINQLLKDPILQVKELAALPDGQEKLDLFIKIFNIE 420 Query: 421 EQLGSDPDG 429 EQL ++ + Sbjct: 421 EQLQTEAEA 429 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,475,723,716 Number of extensions: 97973709 Number of successful extensions: 287531 Number of sequences better than 1.0e-05: 1153 Number of HSP's gapped: 285552 Number of HSP's successfully gapped: 1167 Length of query: 463 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 324 Effective length of database: 1,751,175,118 Effective search space: 567380738232 Effective search space used: 567380738232 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)