BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0176.0008.p (261 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL11.1 B3KL11_9BACI Siroheme synthase EC=1.3.1.76 145 3e-33 O69109.1 O69109_PAEMA Siroheme synthase 125 5e-27 C5D595.1 C5D595_9BACI Siroheme synthase 102 5e-20 Q49UM2.1 Q49UM2_STAS1 Putative uncharacterized protein 100 1e-19 Q4L9E9.1 Q4L9E9_STAHJ Similar to uroporphyrin-III C-methyltransf... 99 3e-19 >B3KL11.1 B3KL11_9BACI Siroheme synthase EC=1.3.1.76 Length = 218 Score = 145 bits (367), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 82/212 (38%), Positives = 110/212 (51%), Gaps = 1/212 (0%) Query: 50 YLPIMLDVQDQRVVVIGGGAVAERKXXXXXXXXXXXXXXSPXXXXXXXXXXXXGR-LSWI 108 Y+P++L+ + + +++GGG VAERK SP + L W Sbjct: 6 YVPVLLNCRGRLCLIVGGGKVAERKALTLLNAGASVHIVSPVLRSETLRHLAGEKALQWT 65 Query: 109 SRPYAPGDIRGAFLVYXXXXXXXXXXXXXXXXRSLGLPVNVASRSEAGSFITPGVLRRGR 168 R Y P D GAF+V+ LG+ VNVAS EAG+FI P VLRRGR Sbjct: 66 ERKYRPDDPAGAFMVHAATDDAKLNLKIAEDADRLGVLVNVASDGEAGTFINPTVLRRGR 125 Query: 169 LTVAVSTSGAGPSAAARITEQLAEILGEEYEPYLDWLHDLRTEIKXXXXXXXXXXXXXXX 228 L +AVSTSGAGP AA I ++L E G EYE YLD+L+ +R+ IK Sbjct: 126 LMLAVSTSGAGPMAARDIRDELEERFGPEYEDYLDFLYRMRSMIKDEVKSADARRRLLQK 185 Query: 229 VGNLDVLNEMRRGTFIEWSADDIDNWITRNLE 260 +D+L E+RRG ++ WS D+I WI +N E Sbjct: 186 AYRMDMLGEIRRGGYVPWSPDEIKQWIEKNQE 217 >O69109.1 O69109_PAEMA Siroheme synthase Length = 218 Score = 125 bits (313), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 70/210 (33%), Positives = 105/210 (50%), Gaps = 2/210 (0%) Query: 47 MVRYLPIMLDVQDQRVVVIGGGAVAERKXXXXXXXXXXXXXXSPXXXXXXXXXXXXGRLS 106 M Y+P+ML+++ +R V++GGG VA RK SP G ++ Sbjct: 1 MPHYMPVMLNLEGRRCVIVGGGTVAARKAAALTEAGAVVTVISPGVTAWLQDRVREGEIA 60 Query: 107 WISRPYAPGDIRGAFLVYXXXXXXXXXXXXXXXXRSLGLPVN-VASRSEAGSFITPGVLR 165 W++R Y GD++GAFLV+ LG+PVN A A F P V+R Sbjct: 61 WLAREYREGDLKGAFLVFAATDSRQVNDSIVKEAEMLGIPVNDTADGRRARKFYHPHVVR 120 Query: 166 RGRLTVAVSTSGAGPSAAARITEQLAEILGEEYEPYLDWLHDLRTEIKXXXXXXXXXXXX 225 RG+L + VSTSGAGP+AA + ++ G+ YE Y+++L +RT +K Sbjct: 121 RGKLVIGVSTSGAGPAAARELCREIDRRFGDTYEQYVEFLSLVRTRVKQQVEDKERRKRL 180 Query: 226 XXXVGNLDVLNEMRRGTFIEWSADDIDNWI 255 +G LD+L +R+G F A++I WI Sbjct: 181 LARLGELDILPSIRQGGFTR-GANEIAAWI 209 >C5D595.1 C5D595_9BACI Siroheme synthase Length = 212 Score = 102 bits (253), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 54/158 (34%), Positives = 81/158 (51%), Gaps = 1/158 (0%) Query: 52 PIMLDVQDQRVVVIGGGAVAERKXXXXXXXXXXXXXXSPXXXXXXXXXXXXGRLSWISRP 111 P+ML++ +Q VVVIGGG VAERK +P G+L W + Sbjct: 4 PVMLNIANQHVVVIGGGKVAERKIHGLLEAKANITVVAPTITSGIQQLEAAGKLIWRQKT 63 Query: 112 YAPGDIRGAFLVYXXXXXXXXXXXXXXXXRSLGLPVNVASRSEAGSFITPGVLRRGRLTV 171 ++P D+ A++V +S L +N+A E +F P V+RRGRLT+ Sbjct: 64 FSPDDLEEAWVVIAATNNRDVNEAVAKAAKSYQL-INIADDPERSNFHVPAVIRRGRLTI 122 Query: 172 AVSTSGAGPSAAARITEQLAEILGEEYEPYLDWLHDLR 209 A+ST GA P+ A +I QL E+ ++Y Y+D+L+ R Sbjct: 123 AISTGGASPTIAQQIRRQLEELYDDDYGRYIDFLYQCR 160 >Q49UM2.1 Q49UM2_STAS1 Putative uncharacterized protein Length = 203 Score = 100 bits (249), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 50/164 (30%), Positives = 82/164 (50%), Gaps = 1/164 (0%) Query: 50 YLPIMLDVQDQRVVVIGGGAVAERKXXXXXXXXXXXXXXSPXXXXXXXXXXXXGRLSWIS 109 ++P+M+D+ ++RVV++GGG VAER+ SP G +SW + Sbjct: 3 FIPLMIDISNKRVVIVGGGQVAERRIATLVNYTTHIKVISPSLTVALYHRYEQGEMSWCA 62 Query: 110 RPYAPGDIRGAFLVYXXXXXXXXXXXXXXXXRSLGLPVNVASRSEAGSFITPGVLRRGRL 169 + + DI A L+ L +N+ S++ G + P +LRRGRL Sbjct: 63 KAFEVNDIAEADLIIAATNDNRVNQQIAASKPQHAL-LNMTSKAHTGDVVFPSILRRGRL 121 Query: 170 TVAVSTSGAGPSAAARITEQLAEILGEEYEPYLDWLHDLRTEIK 213 T+++ST+GA P+ A+I E+ YE Y+D+L++ R IK Sbjct: 122 TLSISTNGASPTLTAQILEEFKARFDFSYEAYVDFLYEYRQHIK 165 >Q4L9E9.1 Q4L9E9_STAHJ Similar to uroporphyrin-III C-methyltransferase Length = 203 Score = 99.4 bits (246), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 57/196 (29%), Positives = 89/196 (45%), Gaps = 1/196 (0%) Query: 51 LPIMLDVQDQRVVVIGGGAVAERKXXXXXXXXXXXXXXSPXXXXXXXXXXXXGRLSWISR 110 +P+ +D+Q++++V++GGG VA R+ SP RL+W + Sbjct: 4 MPLTIDLQNKKIVIVGGGKVATRRANSLVEYCSHVHIVSPSITKDLHTLFQQQRLTWAEK 63 Query: 111 PYAPGDIRGAFLVYXXXXXXXXXXXXXXXXRSLGLPVNVASRSEAGSFITPGVLRRGRLT 170 + P DI AFLV G +N A+ + AG+ P +LRRG+LT Sbjct: 64 MFEPQDISHAFLVIIATNQPEVNQHVKDSLPE-GTLLNHAADASAGNVTFPSMLRRGKLT 122 Query: 171 VAVSTSGAGPSAAARITEQLAEILGEEYEPYLDWLHDLRTEIKXXXXXXXXXXXXXXXVG 230 ++VST GA P +I L + E YE Y+D+L++ R IK V Sbjct: 123 ISVSTDGASPKLGRQIISTLQDTYDERYESYVDFLYESRQLIKASTIEPSRKQALLQHVL 182 Query: 231 NLDVLNEMRRGTFIEW 246 + D L+E ++ F W Sbjct: 183 SEDYLDEEKQKEFKRW 198 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,272,004,424 Number of extensions: 39111575 Number of successful extensions: 107064 Number of sequences better than 1.0e-05: 471 Number of HSP's gapped: 106622 Number of HSP's successfully gapped: 471 Length of query: 261 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 128 Effective length of database: 1,807,701,208 Effective search space: 231385754624 Effective search space used: 231385754624 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)