BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0176.0007.p (316 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL12.1 B3KL12_9BACI Porphobilinogen deaminase EC=2.5.1.61 431 e-119 B1DDT0.1 B1DDT0_9BACL Porphobilinogen deaminase EC=2.5.1.61 414 e-114 O69110.1 HEM3_PAEMA Porphobilinogen deaminase EC=2.5.1.61 381 e-104 Q9K8G0.1 HEM3_BACHD Porphobilinogen deaminase EC=2.5.1.61 348 3e-94 Q65GK1.1 HEM3_BACLD Porphobilinogen deaminase EC=2.5.1.61 346 2e-93 >B3KL12.1 B3KL12_9BACI Porphobilinogen deaminase EC=2.5.1.61 Length = 318 Score = 431 bits (1109), Expect = e-119, Method: Compositional matrix adjust. Identities = 220/318 (69%), Positives = 252/318 (79%), Gaps = 2/318 (0%) Query: 1 MRKIIVGSRQSALALTQTGQVIAELEWLSGEHGFGFTFEVHKIVTKGDRILDVTLSKVGG 60 MR I+VGSRQSALALTQTGQVI +L+ L+ EHG + F V KIVTKGDRILDVTLSKVGG Sbjct: 1 MRTIVVGSRQSALALTQTGQVIDDLKRLAKEHGLEYDFAVKKIVTKGDRILDVTLSKVGG 60 Query: 61 KGLFVKEIEQAMLAREIDMAVHSMKDMPSELPEGLMNGAVPRRVDPRDCLISSGGLTLEE 120 KGLFVKEIEQAM+ EIDMAVHSMKDMPS LPEGL++GAVP R DPRDCLIS G TL++ Sbjct: 61 KGLFVKEIEQAMIDGEIDMAVHSMKDMPSVLPEGLVSGAVPARKDPRDCLISKEGTTLDD 120 Query: 121 LPQGARVGTXXXXXXXXXXXXXPDLKIEPVRGNIDSRLRKLESGEFDAILLAVAGLSRMG 180 LPQGA+VGT PDL+I+ +RGNIDSR+RK+E GEF AI+LA AGL RMG Sbjct: 121 LPQGAKVGTSSLRRSSQLKAYRPDLQIDWIRGNIDSRIRKMEEGEFQAIILATAGLQRMG 180 Query: 181 WEDRVTAYLPPEVCLPAVGQGALGIECRADDDELRKLLALYNDEQTALTVAAERTFLGAL 240 WEDR+T+YLP EV LPAVGQGALGIECR +D+EL LL+LYNDE TA TVAAER FL L Sbjct: 181 WEDRITSYLPVEVSLPAVGQGALGIECRENDEELLHLLSLYNDEVTARTVAAERKFLSEL 240 Query: 241 NGGCQVPIGAFAVL-DAEAAEP-ASPKLITLTGMVGTPDGSVVLKETCSGHDPVRLGAEV 298 NGGCQVPIGA+AV+ + EA + +I LTGMVG+PDG +VLKE+ G DP LG EV Sbjct: 241 NGGCQVPIGAYAVMKNVEAGDSRGEQNVIQLTGMVGSPDGEIVLKESAEGTDPEALGVEV 300 Query: 299 AQKLIARGAEQILADARG 316 A KL A+GAE+ILA RG Sbjct: 301 AAKLKAKGAEKILAQVRG 318 >B1DDT0.1 B1DDT0_9BACL Porphobilinogen deaminase EC=2.5.1.61 Length = 323 Score = 414 bits (1064), Expect = e-114, Method: Compositional matrix adjust. Identities = 206/315 (65%), Positives = 250/315 (79%) Query: 2 RKIIVGSRQSALALTQTGQVIAELEWLSGEHGFGFTFEVHKIVTKGDRILDVTLSKVGGK 61 R I+VG+RQSALALTQTGQVI EL+ +S +HGF + FE+ KIVTKGDRILDVTLSKVGGK Sbjct: 9 RTIVVGTRQSALALTQTGQVIDELKRISEKHGFDYHFEIRKIVTKGDRILDVTLSKVGGK 68 Query: 62 GLFVKEIEQAMLAREIDMAVHSMKDMPSELPEGLMNGAVPRRVDPRDCLISSGGLTLEEL 121 GLFVKEIEQA+L EIDMAVHSMKDMP ELPEGLMNGA PRRVDPRDCLI G +L++L Sbjct: 69 GLFVKEIEQALLDGEIDMAVHSMKDMPYELPEGLMNGATPRRVDPRDCLIMKQGSSLDDL 128 Query: 122 PQGARVGTXXXXXXXXXXXXXPDLKIEPVRGNIDSRLRKLESGEFDAILLAVAGLSRMGW 181 P GARVGT PDLK++ +RGNIDSR+RKLE+ FDA++LA AGLSRMGW Sbjct: 129 PAGARVGTSSLRRSSQLKNIRPDLKLDSIRGNIDSRIRKLETEGFDAVVLAAAGLSRMGW 188 Query: 182 EDRVTAYLPPEVCLPAVGQGALGIECRADDDELRKLLALYNDEQTALTVAAERTFLGALN 241 +DR++A++P ++ +PAVGQGALGIECR D +R+LL L ND +T+L+V AER+FLGALN Sbjct: 189 QDRISAFIPVDLSIPAVGQGALGIECRTADAGVRELLNLLNDTETSLSVRAERSFLGALN 248 Query: 242 GGCQVPIGAFAVLDAEAAEPASPKLITLTGMVGTPDGSVVLKETCSGHDPVRLGAEVAQK 301 GGCQVPIGAFAV+ ++ + + LTGMVG+PDG V+LKE G DP +LG +VA Sbjct: 249 GGCQVPIGAFAVVHKKSEDSFDNAELELTGMVGSPDGEVMLKEIRKGTDPEQLGRDVAAA 308 Query: 302 LIARGAEQILADARG 316 L ARGA++ILA+ G Sbjct: 309 LSARGADRILAEFGG 323 >O69110.1 HEM3_PAEMA Porphobilinogen deaminase EC=2.5.1.61 Length = 324 Score = 381 bits (978), Expect = e-104, Method: Compositional matrix adjust. Identities = 204/317 (64%), Positives = 226/317 (71%), Gaps = 12/317 (3%) Query: 2 RKIIVGSRQSALALTQTGQVIAELEWLSGEHGFGFTFEVHKIVTKGDRILDVTLSKVGGK 61 R I+VG+RQS LALTQT QVI +L+ L HG F FE+ KIVTKGDRILDVTLSK+GGK Sbjct: 4 RTIVVGTRQSQLALTQTEQVIGDLKELCRAHGLPFEFEIKKIVTKGDRILDVTLSKIGGK 63 Query: 62 GLFVKEIEQAMLAREIDMAVHSMKDMPSELPEGLMNGAVPRRVDPRDCLISSGGLTLEEL 121 GLFVKEIEQAML EIDMAVHSMKDMPS LPEGL+NG VP R DPRD LIS GL L EL Sbjct: 64 GLFVKEIEQAMLDGEIDMAVHSMKDMPSVLPEGLINGGVPLRKDPRDALISRSGLHLHEL 123 Query: 122 PQGARVGTXXXXXXXXXXXXXPDLKIEPVRGNIDSRLRKLESGEFDAILLAVAGLSRMGW 181 PQGARVGT PDL +EPVRGNIDSRLRKLE FDAI+LA AGL RMGW Sbjct: 124 PQGARVGTSTLRRSSQLLAYRPDLVLEPVRGNIDSRLRKLEEEGFDAIILAAAGLQRMGW 183 Query: 182 EDRVTAYLPPEVCLPAVGQGALGIECRADDDELRKLLALYNDEQTALTVAAERTFLGALN 241 E+RVTAYL +VCLPAVGQGALGIECR +D ELR +L+LYND TALTVAAER FL LN Sbjct: 184 ENRVTAYLSADVCLPAVGQGALGIECRENDAELRDVLSLYNDPDTALTVAAERRFLAVLN 243 Query: 242 GGCQVPIGAFAVLD---AEAAEPASPKL---------ITLTGMVGTPDGSVVLKETCSGH 289 GGCQVPI A+A L+ +A E I LTGMVG+ DG + C Sbjct: 244 GGCQVPIAAYANLERAGGQAPEEGGRAAASQAPAALRIRLTGMVGSADGRGYFEGECRRE 303 Query: 290 DPVRLGAEVAQKLIARG 306 DPV G +A L+A+G Sbjct: 304 DPVAFGERIAGLLLAQG 320 >Q9K8G0.1 HEM3_BACHD Porphobilinogen deaminase EC=2.5.1.61 Length = 311 Score = 348 bits (893), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 183/316 (57%), Positives = 225/316 (71%), Gaps = 12/316 (3%) Query: 1 MRKIIVGSRQSALALTQTGQVIAELEWLSGEHGFGFTFEVHKIVTKGDRILDVTLSKVGG 60 MRKII+GSR+S LALTQT VI++L+ E G F FE+ KI+TKGD+ILDVTLSKVGG Sbjct: 1 MRKIIIGSRRSQLALTQTEWVISKLK----EAGAPFDFEIKKIITKGDQILDVTLSKVGG 56 Query: 61 KGLFVKEIEQAMLAREIDMAVHSMKDMPSELPEGLMNGAVPRRVDPRDCLISSGGLTLEE 120 KGLFVKEIEQAM +EID+AVHSMKD+PS L EG GA RVDPRD LIS+ G TL E Sbjct: 57 KGLFVKEIEQAMANKEIDIAVHSMKDVPSVLQEGFALGATTTRVDPRDALISNEGYTLAE 116 Query: 121 LPQGARVGTXXXXXXXXXXXXXPDLKIEPVRGNIDSRLRKLESGEFDAILLAVAGLSRMG 180 LP G+ VGT PDL+++ +RGNI++RLRKL+ +FDAI+LA AGL RMG Sbjct: 117 LPAGSIVGTSSLRRSAQILAKRPDLEVKWIRGNIETRLRKLKEEDFDAIILAAAGLERMG 176 Query: 181 WEDR-VTAYLPPEVCLPAVGQGALGIECRADDDELRKLLALYNDEQTALTVAAERTFLGA 239 W + VT YL P++C+PAVGQGALGIECRADDDE+ ++L ND+ TA TV AERTFL Sbjct: 177 WSNEIVTEYLDPDLCVPAVGQGALGIECRADDDEVLEMLKTINDDTTAKTVLAERTFLHR 236 Query: 240 LNGGCQVPIGAFAVLDAEAAEPASPKLITLTGMVGTPDGSVVLKETCSGHDPVRLGAEVA 299 + GGCQVPI +A L E I LT +VGTPDG + KE G DPV+LG +A Sbjct: 237 MEGGCQVPIAGYATLTDEGE-------IELTALVGTPDGEQIFKEILRGKDPVQLGETMA 289 Query: 300 QKLIARGAEQILADAR 315 Q L+ +GA+++L + Sbjct: 290 QALMDQGAKEVLEQVK 305 >Q65GK1.1 HEM3_BACLD Porphobilinogen deaminase EC=2.5.1.61 Length = 313 Score = 346 bits (887), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 184/312 (58%), Positives = 221/312 (70%), Gaps = 12/312 (3%) Query: 1 MRKIIVGSRQSALALTQTGQVIAELEWLSGEHGFGFTFEVHKIVTKGDRILDVTLSKVGG 60 MR IIVGSR+S LA+TQT VI +LE L+ + FTFE+ +IVTKGDRILDVTLSKVGG Sbjct: 1 MRNIIVGSRRSKLAMTQTKWVIKKLEELNPD----FTFEIKEIVTKGDRILDVTLSKVGG 56 Query: 61 KGLFVKEIEQAMLAREIDMAVHSMKDMPSELPEGLMNGAVPRRVDPRDCLISSGGLTLEE 120 KGLFVKEIEQAML+ EIDMAVHSMKDMPS LPEGLM G +P+R D RD LIS G L + Sbjct: 57 KGLFVKEIEQAMLSGEIDMAVHSMKDMPSVLPEGLMVGCIPKREDARDVLISKGRQKLAD 116 Query: 121 LPQGARVGTXXXXXXXXXXXXXPDLKIEPVRGNIDSRLRKLESGEFDAILLAVAGLSRMG 180 L QGA VGT PDL+I+ +RGNID+RL+KLE+ ++DAI+LA AGLSRMG Sbjct: 117 LKQGAVVGTSSLRRSAQLLQMRPDLEIKWIRGNIDTRLKKLETEDYDAIILAAAGLSRMG 176 Query: 181 WEDR-VTAYLPPEVCLPAVGQGALGIECRADDDELRKLLALYNDEQTALTVAAERTFLGA 239 W+D VT +L PE CLPAVGQGAL IECR D+EL L + ND T LTV AER FL A Sbjct: 177 WKDDVVTEFLDPESCLPAVGQGALAIECRGSDEELLSLFSRLNDRYTQLTVTAERAFLHA 236 Query: 240 LNGGCQVPIGAFAVLDAEAAEPASPKLITLTGMVGTPDGSVVLKETCSGHDPVRLGAEVA 299 + GGCQVPI FA +D + +I LTG+V +PDG V +ET G DP +G E A Sbjct: 237 MEGGCQVPIAGFAKMDEDG-------VIELTGLVASPDGKTVYRETVKGKDPEAVGRECA 289 Query: 300 QKLIARGAEQIL 311 + +GA+ ++ Sbjct: 290 AVMSEKGAKALI 301 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,794,451,025 Number of extensions: 73967950 Number of successful extensions: 198229 Number of sequences better than 1.0e-05: 1488 Number of HSP's gapped: 195399 Number of HSP's successfully gapped: 1504 Length of query: 316 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 181 Effective length of database: 1,788,859,178 Effective search space: 323783511218 Effective search space used: 323783511218 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)