BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0176.0006.p (515 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL13.1 B3KL13_9BACI Uroporphyrin-III C-methyltransferase 684 0.0 B1DDS9.1 B1DDS9_9BACL Uroporphyrin-III C-methyltransferase 620 e-175 O69111.1 O69111_PAEMA Uroporphyrinogen-III methyltransferase/uro... 568 e-160 A9U865.1 A9U865_PHYPA Predicted protein (Fragment) 523 e-146 C1TGV6.1 C1TGV6_9FIRM Uroporphyrinogen-III C-methyltransferase; ... 487 e-135 >B3KL13.1 B3KL13_9BACI Uroporphyrin-III C-methyltransferase Length = 519 Score = 684 bits (1765), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/511 (68%), Positives = 390/511 (76%) Query: 1 MTGKVYLVGAGPGDAKLITVKGLECVQKADVLVYDRLASPRLLKWMKPGGQKIYVGKLPD 60 M GKVYLVGAGPGDA+LIT+KG EC++KADV+VYDRLASPRLL+WMKPG +K+YVGKLPD Sbjct: 1 MAGKVYLVGAGPGDARLITIKGKECIEKADVVVYDRLASPRLLRWMKPGAEKVYVGKLPD 60 Query: 61 RHTMKQEEINQLLVDLALEGKTVVRLKGGDPTIFGRVGEEAELLRRHGILYEIVPGVTSA 120 RHTMKQE+INQLLVD ALEGKTVVRLKGGDPTIFGRVGEEAELL RHGI+YEIVPG+TSA Sbjct: 61 RHTMKQEQINQLLVDYALEGKTVVRLKGGDPTIFGRVGEEAELLYRHGIMYEIVPGITSA 120 Query: 121 ISVPAYAGIPVTHRDMASSLSIITGHESPDKLDHSIHWDKVTNATGTLIFLMGVAKIGYI 180 ISVPAYAGIPVTHRD ASSLSIITGHESP+KLD I WDKVT+ATGT IF+MGVAKIGYI Sbjct: 121 ISVPAYAGIPVTHRDHASSLSIITGHESPEKLDRMIQWDKVTHATGTFIFMMGVAKIGYI 180 Query: 181 SAQLIKHGRPPETPVALVRWGTRADQETLTGTLADIEAKVKAADFQSPAVIVVGEVVLQR 240 S QL+KHGRPPETPVAL+RWGTRA+Q+TLTGTLADIE KVKAADFQ PAVIVVGEVVLQR Sbjct: 181 SRQLMKHGRPPETPVALIRWGTRAEQDTLTGTLADIEDKVKAADFQPPAVIVVGEVVLQR 240 Query: 241 EQLMWAEALPLFGKRIVVTRARSQASELVNRIEELGGEPYEFPVIETRMPEGDRQRXXXX 300 E L WAE LPLFGKRI+VTRARSQAS LV++IEELGGEPYEFPVIET MP Sbjct: 241 EHLKWAERLPLFGKRILVTRARSQASSLVSQIEELGGEPYEFPVIETVMPSKGEGLAGIQ 300 Query: 301 XXXXXXXXXXXVFFTSVNGVEFFWRHLAELKVDVRRLHRXXXXXXXXXXXXXXXQRGLMA 360 VFFTSVNGVEFF+RHL E D+R L G+ A Sbjct: 301 EALDKLGTYDWVFFTSVNGVEFFFRHLKEHGRDIRSLASARIVAVGPATADALGNYGISA 360 Query: 361 EELPGHFQAEGLLEAFGPRLQPGQKVLLPRGDLAREWLADKLRELGLXXXXXXXXXXXXX 420 E + +QAEGL++A+GP LQPGQ+VLLPRGDLAR WL +KL E+GL Sbjct: 361 ELIKERYQAEGLIDAYGPELQPGQRVLLPRGDLARRWLPEKLMEMGLHVTEAVIYENVLC 420 Query: 421 XXXXXXXXXXXXXXRIHAVTFTSSSTVRNFISILRRMGLEDPLPLLAGVKIACIGPVTEQ 480 IHAVTFTSSSTV N + +L+ MG+ +P LL IACIGP+T Q Sbjct: 421 GEDDDELLKLLQDGGIHAVTFTSSSTVTNLLKVLKGMGVAEPADLLKESTIACIGPLTAQ 480 Query: 481 TAVEAGLTPGLLPGEATIEGLVQELCRWNES 511 TA +AGL LL EAT++ LV+ LC WNES Sbjct: 481 TAEKAGLHVSLLAEEATVDSLVKALCVWNES 511 >B1DDS9.1 B1DDS9_9BACL Uroporphyrin-III C-methyltransferase Length = 520 Score = 620 bits (1598), Expect = e-175, Method: Compositional matrix adjust. Identities = 315/513 (61%), Positives = 376/513 (73%) Query: 3 GKVYLVGAGPGDAKLITVKGLECVQKADVLVYDRLASPRLLKWMKPGGQKIYVGKLPDRH 62 G VYLVGAGPGD KLIT++GLEC++++DV+VYDRLASPRLL++MK G +KIYVGKLPDRH Sbjct: 4 GVVYLVGAGPGDPKLITLRGLECLRRSDVVVYDRLASPRLLRYMKEGARKIYVGKLPDRH 63 Query: 63 TMKQEEINQLLVDLALEGKTVVRLKGGDPTIFGRVGEEAELLRRHGILYEIVPGVTSAIS 122 TMKQEEINQLLVDLALEG TV RLKGGDPTIFGRVGEEAELL+ +G+ +EIVPG+TSAI+ Sbjct: 64 TMKQEEINQLLVDLALEGHTVTRLKGGDPTIFGRVGEEAELLQDNGVSFEIVPGITSAIA 123 Query: 123 VPAYAGIPVTHRDMASSLSIITGHESPDKLDHSIHWDKVTNATGTLIFLMGVAKIGYISA 182 VPAYAGIPVTHRD+ASSLSI+TGHESP+KLD +IHWDKVTNATGTLIFLMGVAKIGYI+ Sbjct: 124 VPAYAGIPVTHRDLASSLSIVTGHESPEKLDRTIHWDKVTNATGTLIFLMGVAKIGYIAE 183 Query: 183 QLIKHGRPPETPVALVRWGTRADQETLTGTLADIEAKVKAADFQSPAVIVVGEVVLQREQ 242 QLI+HG+P TPVAL+RWGTR +Q+T+ GTL IE VK A+FQ PAVIVVG+VVLQR++ Sbjct: 184 QLIRHGKPATTPVALIRWGTRVEQQTIVGTLESIERIVKEANFQPPAVIVVGDVVLQRDK 243 Query: 243 LMWAEALPLFGKRIVVTRARSQASELVNRIEELGGEPYEFPVIETRMPEGDRQRXXXXXX 302 L W E PLFG R++VTRAR+QAS+L +RIEELGGEP E PVIETR P + Sbjct: 244 LKWYEKKPLFGVRVLVTRARAQASDLADRIEELGGEPCELPVIETREPRDEAAVEGIRTA 303 Query: 303 XXXXXXXXXVFFTSVNGVEFFWRHLAELKVDVRRLHRXXXXXXXXXXXXXXXQRGLMAEE 362 + FTSVNGVE+F+ L VD+RR H +RGL+A++ Sbjct: 304 LAGAESYNWIMFTSVNGVEYFFNWLDRFNVDIRRFHAARIAAVGPRTAEALRKRGLIADQ 363 Query: 363 LPGHFQAEGLLEAFGPRLQPGQKVLLPRGDLAREWLADKLRELGLXXXXXXXXXXXXXXX 422 LP FQAEGLLE L+PG+KVLLPRGDLARE L +L+ GL Sbjct: 364 LPAKFQAEGLLEQLEGELRPGEKVLLPRGDLAREILPRELKAKGLVPVELDVYETVIADS 423 Query: 423 XXXXXXXXXXXXRIHAVTFTSSSTVRNFISILRRMGLEDPLPLLAGVKIACIGPVTEQTA 482 IHA+TFTSSSTV N + +LRR G EDP+ LAG+ + IGPVT +TA Sbjct: 424 QDEQAFEWIRNREIHAITFTSSSTVTNLLELLRRNGFEDPVAQLAGIDVVSIGPVTSKTA 483 Query: 483 VEAGLTPGLLPGEATIEGLVQELCRWNESTRLR 515 EAGL+ L P ATIE LV+ L + + RL+ Sbjct: 484 SEAGLSVTLEPENATIEALVEALVQHQITKRLK 516 >O69111.1 O69111_PAEMA Uroporphyrinogen-III methyltransferase/uroporphyrinogen-III synthase EC=2.1.1.107 EC=4.2.1.75 Length = 511 Score = 568 bits (1464), Expect = e-160, Method: Compositional matrix adjust. Identities = 307/511 (60%), Positives = 363/511 (71%), Gaps = 8/511 (1%) Query: 1 MTGKVYLVGAGPGDAKLITVKGLECVQKADVLVYDRLASPRLLKWMKPGGQKIYVGKLPD 60 MTG+VYLVGAGPGDA+LIT+KGL+C++K DV+VYDRLA+P LL+ KPG +KIYVGK D Sbjct: 3 MTGRVYLVGAGPGDARLITLKGLDCIEKGDVIVYDRLANPSLLRKAKPGAEKIYVGKRTD 62 Query: 61 RHTMKQEEINQLLVDLALEGKTVVRLKGGDPTIFGRVGEEAELLRRHGILYEIVPGVTSA 120 RHTMKQE+I++LLVDLAL GKTVVRLKGGDPT+FGR GEEAELLR+HGI YEIVPG+T+A Sbjct: 63 RHTMKQEDISRLLVDLALAGKTVVRLKGGDPTVFGRGGEEAELLRKHGIPYEIVPGITAA 122 Query: 121 ISVPAYAGIPVTHRDMASSLSIITGHESPDKLDHSIHWDKVTNATGTLIFLMGVAKIGYI 180 ISVPAYAGIPVTHRD+ASS+SII GHESPDKLD SI+W+KVTNATGTL+F+MGVA IGYI Sbjct: 123 ISVPAYAGIPVTHRDLASSVSIIAGHESPDKLDLSINWEKVTNATGTLVFMMGVANIGYI 182 Query: 181 SAQLIKHGRPPETPVALVRWGTRADQETLTGTLADIEAKVKAADFQSPAVIVVGEVVLQR 240 S QLI HGRPPETPVALVRWGTRA+QETLTGTLADIE KV + F+ V VVGEVV QR Sbjct: 183 SKQLIAHGRPPETPVALVRWGTRAEQETLTGTLADIEQKVLDSGFKR-RVTVVGEVVNQR 241 Query: 241 EQLMWAEALPLFGKRIVVTRARSQASELVNRIEELGGEPYEFPVIETRMPEGDRQRXXXX 300 EQL+WAEA+PLFGKRI+VTR+RSQAS+LV IEELGGEPYEFPVI+ MP G Sbjct: 242 EQLLWAEAMPLFGKRILVTRSRSQASDLVRGIEELGGEPYEFPVIQISMPSGA-ALERTD 300 Query: 301 XXXXXXXXXXXVFFTSVNGVEFFWRHLAELKVDVRRLHRXXXXXXXXXXXXXXXQRGLMA 360 FFTSVNGV++F++HL L D+R L++ +RG+ A Sbjct: 301 EALSRLETYDWAFFTSVNGVDYFFKHLERLGKDIRALYK--AIAAVGGHAGALRERGIAA 358 Query: 361 EELPGHFQAEGLLEAFGPRLQPGQKVLLPRGDL-AREWLADKLRELGLXXXXXXXXXXXX 419 EELPG FQAE + L P + G L R + ++ G Sbjct: 359 EELPGRFQAEPCWKRTARSLCPAKAYCCRTGILPGRGCRSCAIK--GWPSPRRSCMRRVP 416 Query: 420 XXXXXXXXXXXXXXXRIHAVTFTSSSTVRNFISILRRMGLEDPLPLLAGVKIACIGPVTE 479 RIHAVTFTSSSTV + ++ L MG+ P+ L G +IACIGPVT Sbjct: 417 AGEKDEELIKLLEKGRIHAVTFTSSSTVTHLLAALSGMGVR-PVAALRGREIACIGPVTA 475 Query: 480 QTAVEAGLTPGLLPGEATIEGLVQELCRWNE 510 +TA EAGL +L EATI L++ LC W E Sbjct: 476 RTAEEAGLRVSILAKEATIPSLIEALCDWKE 506 >A9U865.1 A9U865_PHYPA Predicted protein (Fragment) Length = 344 Score = 523 bits (1346), Expect = e-146, Method: Compositional matrix adjust. Identities = 257/339 (75%), Positives = 290/339 (85%) Query: 1 MTGKVYLVGAGPGDAKLITVKGLECVQKADVLVYDRLASPRLLKWMKPGGQKIYVGKLPD 60 M G+V+LVGAGPG AKLITVKGLEC+QKADV+VYDRLA P+LL +KPG +KIYVGKLPD Sbjct: 1 MAGRVFLVGAGPGHAKLITVKGLECLQKADVVVYDRLAGPQLLHSLKPGTEKIYVGKLPD 60 Query: 61 RHTMKQEEINQLLVDLALEGKTVVRLKGGDPTIFGRVGEEAELLRRHGILYEIVPGVTSA 120 RHTMKQEEINQLLVDLAL+GKTVVRLKGGDPTIFGRVGEEAELLRR+GI YEIVPG+T+A Sbjct: 61 RHTMKQEEINQLLVDLALQGKTVVRLKGGDPTIFGRVGEEAELLRRNGIPYEIVPGITAA 120 Query: 121 ISVPAYAGIPVTHRDMASSLSIITGHESPDKLDHSIHWDKVTNATGTLIFLMGVAKIGYI 180 I VPAYAGIPVTHRD+ASSLSIITGHESPDKLD I WDKVTNATGTL+F+MGVAKIGYI Sbjct: 121 IGVPAYAGIPVTHRDLASSLSIITGHESPDKLDRMIEWDKVTNATGTLVFMMGVAKIGYI 180 Query: 181 SAQLIKHGRPPETPVALVRWGTRADQETLTGTLADIEAKVKAADFQSPAVIVVGEVVLQR 240 + QL+++GR P+TPVAL+RWGTRA+Q L GTLADI KVK ADFQ PAVIVVG+VV QR Sbjct: 181 AEQLMRYGRKPDTPVALIRWGTRAEQAVLAGTLADIAEKVKQADFQPPAVIVVGDVVRQR 240 Query: 241 EQLMWAEALPLFGKRIVVTRARSQASELVNRIEELGGEPYEFPVIETRMPEGDRQRXXXX 300 EQL WAE+ PLFGKRI+VTR+R+QAS+LVNRIEELGGEPYEFPVIET PE + Sbjct: 241 EQLKWAESQPLFGKRILVTRSRAQASDLVNRIEELGGEPYEFPVIETVYPEAPELKQGLK 300 Query: 301 XXXXXXXXXXXVFFTSVNGVEFFWRHLAELKVDVRRLHR 339 VFFTS NGV++F+RHL E D+R+LHR Sbjct: 301 DAFDRLGEYDWVFFTSTNGVDYFFRHLKEQGKDIRQLHR 339 >C1TGV6.1 C1TGV6_9FIRM Uroporphyrinogen-III C-methyltransferase; uroporphyrinogen-III synthase EC=2.1.1.107 EC=4.2.1.75 Length = 505 Score = 487 bits (1254), Expect = e-135, Method: Compositional matrix adjust. Identities = 261/506 (51%), Positives = 325/506 (64%), Gaps = 6/506 (1%) Query: 3 GKVYLVGAGPGDAKLITVKGLECVQKADVLVYDRLASPRLLKWMKPGGQKIYVGKLPDRH 62 G VYL+GAGPGD KLITV+G+EC++KADV+VYDRLA RLL + P + IYVGK PDRH Sbjct: 4 GIVYLIGAGPGDPKLITVRGVECIKKADVVVYDRLAGSRLLGYAAPHAEMIYVGKSPDRH 63 Query: 63 TMKQEEINQLLVDLALEGKTVVRLKGGDPTIFGRVGEEAELLRRHGILYEIVPGVTSAIS 122 T++QEEINQLLV A +G V RLKGGDP +FGR GEEAE+L ++ I +E+VPG+TSAI+ Sbjct: 64 TLRQEEINQLLVKKAAQGLVVARLKGGDPFVFGRGGEEAEVLVQNSIPFEVVPGITSAIA 123 Query: 123 VPAYAGIPVTHRDMASSLSIITGHESPDKLDHSIHWDKVTNATGTLIFLMGVAKIGYISA 182 VPAYAGIPVTHRD S+L+IITG+E P K D SI WDK+ A GTL+FLMG+ + I+ Sbjct: 124 VPAYAGIPVTHRDCTSTLAIITGNEDPAKEDSSIAWDKIATAAGTLVFLMGMGNLPKITD 183 Query: 183 QLIKHGRPPETPVALVRWGTRADQETLTGTLADIEAKVKAADFQSPAVIVVGEVVLQREQ 242 +L+ GRP TPVAL+RWGTR +Q TLTGTL DI K DF++PA+I+VGEVV RE+ Sbjct: 184 RLMGAGRPASTPVALIRWGTRPEQRTLTGTLEDIADLAKQDDFKNPAIIIVGEVVKLREK 243 Query: 243 LMWAEALPLFGKRIVVTRARSQASELVNRIEELGGEPYEFPVIETRMPEGDRQRXXXXXX 302 L W E PLFGKRIVVTR+R QAS L IE LGGE +EFP I+ PE Sbjct: 244 LAWFENKPLFGKRIVVTRSREQASVLSEAIEALGGESWEFPAIKIEEPE---DYTAMDSS 300 Query: 303 XXXXXXXXXVFFTSVNGVEFFWRHLAELKVDVRRLHRXXXXXXXXXXXXXXXQRGLMAEE 362 + FTSVNGV+++ L + K D+R L Q GL+ + Sbjct: 301 IENIVQFDWLIFTSVNGVKYYMDRLKQHKKDIRELKGIRLCAIGPKTKESLEQYGLLVDY 360 Query: 363 LPGHFQAEGLLEAFGPRLQPGQKVLLPRGDLAREWLADKLRELGLXXXXXXXXXXXXXXX 422 +PG ++AE ++E ++ PG KVLL R D+AR+ L + LREL Sbjct: 361 VPGEYRAEQIIEGLKDKVSPGTKVLLARADIARKVLPEALRELKADVTEVVAYRTLQGSS 420 Query: 423 XXXXXXXXXXXXRIHAVTFTSSSTVRNFISILRRMGLEDPLPLLAGVKIACIGPVTEQTA 482 I AVTFTSSSTVRNF+ +L G E+ + LL GV +A IGPVT QTA Sbjct: 421 SSELLKQMLVDKEIDAVTFTSSSTVRNFVDLL---GKENRVELLNGVVLASIGPVTSQTA 477 Query: 483 VEAGLTPGLLPGEATIEGLVQELCRW 508 VE GL + E TI GLVQEL + Sbjct: 478 VEMGLNIDIEAKEYTINGLVQELLAY 503 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,765,622,403 Number of extensions: 105593478 Number of successful extensions: 263038 Number of sequences better than 1.0e-05: 2782 Number of HSP's gapped: 258492 Number of HSP's successfully gapped: 2791 Length of query: 515 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 375 Effective length of database: 1,741,754,103 Effective search space: 653157788625 Effective search space used: 653157788625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)