BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0174.0004.p (470 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8D1V0.1 B8D1V0_HALOH Glycoside hydrolase family 30 597 e-169 C1I3W0.1 C1I3W0_9CLOT Glycoside hydrolase 499 e-139 B1ZPN4.1 B1ZPN4_OPITP Glucosylceramidase EC=3.2.1.45 474 e-132 A4XMF5.1 A4XMF5_CALS8 Glucosylceramidase EC=3.2.1.45 464 e-129 B9MLP9.1 B9MLP9_ANATD Glucosylceramidase EC=3.2.1.45 456 e-126 >B8D1V0.1 B8D1V0_HALOH Glycoside hydrolase family 30 Length = 445 Score = 597 bits (1539), Expect = e-169, Method: Compositional matrix adjust. Identities = 273/445 (61%), Positives = 353/445 (79%) Query: 26 MTKARTVITAKDTGERLSPREGIVFRARNTGEKADVQLLPEREFQQILGFGGAFTEAAAY 85 M ++TA+DTG+RLS + VF++ + D++L P+ +Q+I+GFGGAFTEAAAY Sbjct: 1 MNSISVILTARDTGDRLSLKGEKVFKSGIGRQDIDLELYPDTRYQKIIGFGGAFTEAAAY 60 Query: 86 TLSRMSPGKRAEVIRRYFHPEEGLNYSMGRVHIHSCDFALGNYTYVQDGDSALATFDISH 145 TLS++S KR ++I YF ++GL Y+MGRVHI+SCDFAL NYTYV+DGD L TFDIS Sbjct: 61 TLSKISSDKRLKIIESYFDRDKGLGYNMGRVHINSCDFALENYTYVEDGDRELKTFDISR 120 Query: 146 DHQWVIPLIKDAMDVKGGPLTMLASPWSPPAWMKTNGEMNHGGSLKPEYANVWARYYTKF 205 + QWVIPLI+DA+ +GG + +LASPWSPPAWMK+N MN+GG L PEY +VWA+YYTK+ Sbjct: 121 ERQWVIPLIRDAIKARGGEIKLLASPWSPPAWMKSNENMNYGGKLLPEYRDVWAKYYTKY 180 Query: 206 IEAYRREGIPVWAVSVQNEPAAVQTWDSCVYSAEEERDFVKNHLGPVMHEAGMADVNIVI 265 I+A++ EG+ +W ++VQNEPAAVQTWDSC Y+AEEERDFVKNHLGPVMHE G+ D+NI+I Sbjct: 181 IKAFQEEGLNIWGITVQNEPAAVQTWDSCTYTAEEERDFVKNHLGPVMHEEGLGDINILI 240 Query: 266 WDHNRDIMVERASTVLSDPEAARYVWGTGIHWYGGEEFDKVARVHELFPEKHLLFTEGCQ 325 WDHNRDI+V+R +L D EAA+YVWGT HWY E+FD V +VHE++P+KHLLFTEGCQ Sbjct: 241 WDHNRDIIVDRVKPILDDLEAAKYVWGTAFHWYVSEDFDNVGQVHEMYPDKHLLFTEGCQ 300 Query: 326 EGGVRLGEWFTGERYGRNMIGDLNAWTEGYLDWNLVLDETGGPNHVGNFCDAPVIADTTS 385 EGG ++GEWFTGERYGRN+IGDLN WTEGYLDWN+VL+E GGPNHVGN+CDAPVI DT + Sbjct: 301 EGGCQIGEWFTGERYGRNIIGDLNNWTEGYLDWNMVLNEEGGPNHVGNYCDAPVIVDTNT 360 Query: 386 DEVHYNSSYYYIGHFSKFIAPGAVRIGLESAAEGVLSTAFRNPDGSLAVVLMNEGEHVRS 445 +E++YNSSYYYIGHFSK+I PGAVRIG+ + + +T+F N DGS+ +++MNE ++ Sbjct: 361 EEIYYNSSYYYIGHFSKYIRPGAVRIGVSCTNDNLKATSFLNSDGSIILIVMNETDNPTD 420 Query: 446 VTXXXXXXXXXXXXPPHSIATHVIS 470 P H+IAT++I+ Sbjct: 421 FAVSLDNKVADLTLPAHAIATYIIT 445 >C1I3W0.1 C1I3W0_9CLOT Glycoside hydrolase Length = 450 Score = 499 bits (1285), Expect = e-139, Method: Compositional matrix adjust. Identities = 221/429 (51%), Positives = 306/429 (71%), Gaps = 5/429 (1%) Query: 22 EMAEMTKARTVITAKDTGERLSP---REGIVFRARNTGEKADVQLLPEREFQQILGFGGA 78 E M IT K+T +RL+ RE IV A K V++ ++ FQ+ILGFGGA Sbjct: 2 ESESMRSYNVYITCKNTDDRLNRYDLREVIVDEAGEINCK--VKVDTKKRFQKILGFGGA 59 Query: 79 FTEAAAYTLSRMSPGKRAEVIRRYFHPEEGLNYSMGRVHIHSCDFALGNYTYVQDGDSAL 138 TE++AY LS++ KR +++ YF E+G+ Y+ RVHI+SCDFAL NY+YV++ D L Sbjct: 60 LTESSAYVLSKIEDEKRKDILEAYFDCEKGIGYNFARVHINSCDFALENYSYVEENDETL 119 Query: 139 ATFDISHDHQWVIPLIKDAMDVKGGPLTMLASPWSPPAWMKTNGEMNHGGSLKPEYANVW 198 +F + ++V+PL+++ D+K L +LASPWSPP++MKTN EMN+GG + +Y +W Sbjct: 120 ESFTLDRAEKYVLPLVREVKDIKKDQLNILASPWSPPSYMKTNNEMNNGGKISNKYKQLW 179 Query: 199 ARYYTKFIEAYRREGIPVWAVSVQNEPAAVQTWDSCVYSAEEERDFVKNHLGPVMHEAGM 258 A YY K+IE R++G +W V+VQNEPAA Q WDSC+YSAEEERDFVK++LGP + + Sbjct: 180 ADYYVKYIEEMRKKGFDIWGVTVQNEPAAKQVWDSCLYSAEEERDFVKDYLGPTFEKNNL 239 Query: 259 ADVNIVIWDHNRDIMVERASTVLSDPEAARYVWGTGIHWYGGEEFDKVARVHELFPEKHL 318 + I+IWDHNRD++ +RA TVL D EA +YVWGTGIHWY E+F+ +++VHE FP+KH+ Sbjct: 240 SHKKIIIWDHNRDLVYDRAKTVLEDKEANKYVWGTGIHWYVSEDFENLSKVHEAFPDKHI 299 Query: 319 LFTEGCQEGGVRLGEWFTGERYGRNMIGDLNAWTEGYLDWNLVLDETGGPNHVGNFCDAP 378 +FTEGCQEGGV LG+W TGERYGRN+IGDLN + E ++DWN+VLDETGGPNHVGN CDAP Sbjct: 300 IFTEGCQEGGVHLGDWNTGERYGRNIIGDLNNYVEAWIDWNIVLDETGGPNHVGNLCDAP 359 Query: 379 VIADTTSDEVHYNSSYYYIGHFSKFIAPGAVRIGLESAAEGVLSTAFRNPDGSLAVVLMN 438 +I D ++V YN+S+YYI HFSKFI ++R+ E E + + + + +L +V+MN Sbjct: 360 IIVDLRKNKVIYNNSFYYISHFSKFIKKDSIRVLSEVDNEKIYALSCIDEKDNLCIVIMN 419 Query: 439 EGEHVRSVT 447 E + +T Sbjct: 420 ENSEDKKIT 428 >B1ZPN4.1 B1ZPN4_OPITP Glucosylceramidase EC=3.2.1.45 Length = 475 Score = 474 bits (1221), Expect = e-132, Method: Compositional matrix adjust. Identities = 220/462 (47%), Positives = 309/462 (66%), Gaps = 11/462 (2%) Query: 15 IMIYKIKEMA--EMTKARTVITAKDTGERLSPREGIVFRARNTGEKADVQLL---PEREF 69 +++ + +A M + TA+D RL+ + D +L P + Sbjct: 14 LLVLTVSALAVTPMRSWSLIETARDNDHRLT---AVALSTNAPAPGTDAPVLRADPNQRR 70 Query: 70 QQILGFGGAFTEAAAYTLSRMSPGKRAEVIRRYFHPEEGLNYSMGRVHIHSCDFALGNYT 129 QQ++GFGGA TE++A+ L+++ KR EV+RRY+ P+EG+ Y++ R HI+SCDF+L ++ Sbjct: 71 QQMVGFGGALTESSAWVLAQLPAEKRLEVLRRYYDPQEGIGYTLARTHINSCDFSLSIWS 130 Query: 130 YVQD-GDSALATFDISHDHQWVIPLIKDAMDVKGGP-LTMLASPWSPPAWMKTNGEMNHG 187 + GD L F ++ +W++PL++DA + G + ASPWSPPAWMKTNG M+ G Sbjct: 131 LDETPGDYYLHDFTLAPMQRWLMPLLRDAYAIAGADRFKLTASPWSPPAWMKTNGRMDSG 190 Query: 188 GSLKPEYANVWARYYTKFIEA-YRREGIPVWAVSVQNEPAAVQTWDSCVYSAEEERDFVK 246 G+L+PEY + WAR+Y KF+EA +R E IPVWA++VQNEP AVQ W+SC++S EEERDFV+ Sbjct: 191 GALRPEYRDAWARFYVKFVEAMHREEKIPVWALTVQNEPEAVQVWESCIFSPEEERDFVR 250 Query: 247 NHLGPVMHEAGMADVNIVIWDHNRDIMVERASTVLSDPEAARYVWGTGIHWYGGEEFDKV 306 +HLGP + +AG+ D+ ++ +DHNRDI +RA+ + DP +A+Y+WG+ IHWY E+F Sbjct: 251 DHLGPTLQKAGLGDMRLIGFDHNRDIFEKRAAALFGDPASAQYLWGSAIHWYVSEDFAAS 310 Query: 307 ARVHELFPEKHLLFTEGCQEGGVRLGEWFTGERYGRNMIGDLNAWTEGYLDWNLVLDETG 366 +RVH FP+K +LFTEGC EGGV+LG W GERY RNMIGD W G++DWN+ LD TG Sbjct: 311 SRVHAAFPQKQILFTEGCWEGGVKLGRWDRGERYARNMIGDFRNWVCGWIDWNIALDRTG 370 Query: 367 GPNHVGNFCDAPVIADTTSDEVHYNSSYYYIGHFSKFIAPGAVRIGLESAAEGVLSTAFR 426 GPNHVGN CDAPVI DT + EVHY SS+YYI HFS+F+APGA + +AA G+ + AF Sbjct: 371 GPNHVGNLCDAPVIVDTATGEVHYQSSFYYIAHFSRFVAPGAHCVESTTAAAGLDTVAFV 430 Query: 427 NPDGSLAVVLMNEGEHVRSVTXXXXXXXXXXXXPPHSIATHV 468 NPDGSL V++N + + P H+I T+V Sbjct: 431 NPDGSLVCVVLNATDTAQPFNLTTDGTALACEIPAHAIQTYV 472 >A4XMF5.1 A4XMF5_CALS8 Glucosylceramidase EC=3.2.1.45 Length = 445 Score = 464 bits (1194), Expect = e-129, Method: Compositional matrix adjust. Identities = 217/444 (48%), Positives = 295/444 (66%), Gaps = 6/444 (1%) Query: 28 KARTVITAKDTGERLSPREGIVFRARNTGEKADVQLLPEREFQQILGFGGAFTEAAAYTL 87 K ITAK G + + I + T E + + P FQ+++GFGGA TEAAA + Sbjct: 4 KIACYITAKQ-GTFMQQVDNIKECDKLTSESV-ITIDPSTTFQKVIGFGGALTEAAAVNI 61 Query: 88 SRMSPGKRAEVIRRYFHPEEGLNYSMGRVHIHSCDFALGNYTYVQ-DGDSALATFDISHD 146 + P ++ E++R YF PE+GL Y + R+H++SCDF + Y+ +GD L F+I D Sbjct: 62 LSLLPHQQDEILRGYFDPEKGLGYKLCRIHMNSCDFCISTYSCDDVEGDVELKHFNIERD 121 Query: 147 HQWVIPLIKDAMDVKGGP-LTMLASPWSPPAWMKTNGEMNHGGSLKPEYANVWARYYTKF 205 + VIPL+K M K P L +L SPWSPPAWMKTN +M HGG LK EY WAR++ KF Sbjct: 122 KKMVIPLLKRIM--KYCPDLKILVSPWSPPAWMKTNNDMCHGGKLKDEYKKTWARFFCKF 179 Query: 206 IEAYRREGIPVWAVSVQNEPAAVQTWDSCVYSAEEERDFVKNHLGPVMHEAGMADVNIVI 265 I+AY+ EGI +WAV+VQNEP A Q W+SC+Y+AEEERDFVK+ LGP + E G++ + I+I Sbjct: 180 IKAYQEEGIDIWAVTVQNEPMATQVWESCIYTAEEERDFVKDCLGPTLEEEGLSHIKILI 239 Query: 266 WDHNRDIMVERASTVLSDPEAARYVWGTGIHWYGGEEFDKVARVHELFPEKHLLFTEGCQ 325 WDHN+DI+ ER T+LSD EAA++VWG HWYGG+ FD++ ++ E FP+ +L+FTEGCQ Sbjct: 240 WDHNKDIIYERVKTILSDKEAAKFVWGVAFHWYGGDHFDQLKKIKEEFPDVNLVFTEGCQ 299 Query: 326 EGGVRLGEWFTGERYGRNMIGDLNAWTEGYLDWNLVLDETGGPNHVGNFCDAPVIADTTS 385 EGGV+LG W GERY +IGD N +T G++DWN+VLD GGPNHVGNFCDAP+I D Sbjct: 300 EGGVKLGSWELGERYAHEIIGDFNNYTIGFMDWNIVLDTVGGPNHVGNFCDAPIIVDKDQ 359 Query: 386 DEVHYNSSYYYIGHFSKFIAPGAVRIGLESAAEGVLSTAFRNPDGSLAVVLMNEGEHVRS 445 +++Y ++YYYIGHFSKFI PGA + ++ + A +N D +LAVV++N+ Sbjct: 360 KKIYYQNAYYYIGHFSKFIKPGAKIVKSSCSSSRLEVLAAKNGDDTLAVVVLNKNPEEIE 419 Query: 446 VTXXXXXXXXXXXXPPHSIATHVI 469 P SI+T V+ Sbjct: 420 FNMVIENKIFCGNSPARSISTVVL 443 >B9MLP9.1 B9MLP9_ANATD Glucosylceramidase EC=3.2.1.45 Length = 446 Score = 456 bits (1172), Expect = e-126, Method: Compositional matrix adjust. Identities = 205/410 (50%), Positives = 281/410 (68%), Gaps = 2/410 (0%) Query: 61 VQLLPEREFQQILGFGGAFTEAAAYTLSRMSPGKRAEVIRRYFHPEEGLNYSMGRVHIHS 120 + + P FQ+++GFGGA TEAAA + + P ++ E++R YF P+EGL Y + R+H++S Sbjct: 36 ITIEPSTTFQEVIGFGGALTEAAAVNILSLLPHQQEEILRGYFDPKEGLGYKLCRIHMNS 95 Query: 121 CDFALGNYTYVQ-DGDSALATFDISHDHQWVIPLIKDAMDVKGGPLTMLASPWSPPAWMK 179 CDF + +Y+ +GD L F+I D + VIPL+K + L +L SPWSPPAWMK Sbjct: 96 CDFCVDSYSCDDVEGDIELKHFNIERDKKMVIPLLKRIKEY-CKDLKILVSPWSPPAWMK 154 Query: 180 TNGEMNHGGSLKPEYANVWARYYTKFIEAYRREGIPVWAVSVQNEPAAVQTWDSCVYSAE 239 TNG+M HGG LK EY WAR++ KFI+AY+ EGI +WAV+VQNEP A Q W+SC+Y+AE Sbjct: 155 TNGDMCHGGKLKDEYKKTWARFFCKFIKAYKEEGIEIWAVTVQNEPMATQVWESCIYTAE 214 Query: 240 EERDFVKNHLGPVMHEAGMADVNIVIWDHNRDIMVERASTVLSDPEAARYVWGTGIHWYG 299 EERDFVK +LGP + E G+ DV I+IWDHN+DI+ +R T+LSD EAA++VWG HWYG Sbjct: 215 EERDFVKYYLGPTLAEEGLGDVKILIWDHNKDIIYDRVKTILSDKEAAKFVWGVAFHWYG 274 Query: 300 GEEFDKVARVHELFPEKHLLFTEGCQEGGVRLGEWFTGERYGRNMIGDLNAWTEGYLDWN 359 G+ FD++ ++ E FP+ +L+FTEGCQEGGV+LG W GERY +IGD N +T G++DWN Sbjct: 275 GDHFDQLKKIKEEFPDVNLVFTEGCQEGGVKLGSWELGERYAHEIIGDFNNYTIGFMDWN 334 Query: 360 LVLDETGGPNHVGNFCDAPVIADTTSDEVHYNSSYYYIGHFSKFIAPGAVRIGLESAAEG 419 +VLD GGPNHVGNFCDAP+I D +++Y ++ YYIGHFSKFI PGA + ++ Sbjct: 335 IVLDTVGGPNHVGNFCDAPIIVDKDQKKIYYQNACYYIGHFSKFIRPGAKVVKSSCSSSK 394 Query: 420 VLSTAFRNPDGSLAVVLMNEGEHVRSVTXXXXXXXXXXXXPPHSIATHVI 469 + A ++ D +LAVV+ N+ + P SI T V+ Sbjct: 395 LEVLAAKSQDDTLAVVVFNKNPEEIEFSIVIGDKIFSGKSPARSILTIVL 444 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,040,532,601 Number of extensions: 135944427 Number of successful extensions: 289270 Number of sequences better than 1.0e-05: 315 Number of HSP's gapped: 287959 Number of HSP's successfully gapped: 332 Length of query: 470 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 331 Effective length of database: 1,751,175,118 Effective search space: 579638964058 Effective search space used: 579638964058 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)