BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0174.0001.p (491 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q6HIE7.1 Q6HIE7_BACHK Putative uncharacterized protein 218 1e-54 B7JPT0.1 B7JPT0_BACC0 Putative penicillin-binding protein 216 5e-54 C3G3D3.1 C3G3D3_BACTU Putative uncharacterized protein 216 5e-54 C3PB44.1 C3PB44_BACAA Putative penicillin-binding protein 215 9e-54 Q81Q59.1 Q81Q59_BACAN Penicillin-binding protein, putative 215 9e-54 >Q6HIE7.1 Q6HIE7_BACHK Putative uncharacterized protein Length = 481 Score = 218 bits (555), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 146/462 (31%), Positives = 247/462 (53%), Gaps = 30/462 (6%) Query: 32 ARIDTFVESMLEKLDFPGMAVGIVKGDQIIYTQGYGHSGPDGQPVTPQTPFILGSSSKSF 91 A +D ++E + + + PG+AV IV ++ +T+ G +G + VT +TPF +GS SKS Sbjct: 35 ATLDKYIEEFINEQNIPGVAVAIVHNKEVFFTKTMGVTGESEKKVTSKTPFAIGSISKSL 94 Query: 92 TALAIMQLVEQGKIDLNAPVQRYLPEFELEDKDASKTILVRHLLNHSSGISTFHG---RT 148 TALAI++L+E KI L PVQRYLP F+L++ S TI ++HLL H+SGI+T+ G Sbjct: 95 TALAIVKLIEDKKIKLEDPVQRYLPWFKLKNPQISSTITIQHLLTHTSGINTYEGLALSD 154 Query: 149 AFTNTASTIDELIHGLKNTPLTEPVGSQYQYSNANYDLLGGIIQAVSGESYAEYIQDHVY 208 + +++ + E + L N +T P G +YQYSNANY +LG +I+ V+ E+Y+ Y++ +++ Sbjct: 155 KQSKSSTALKENVMKLSNVKVTAPPGEKYQYSNANYIVLGALIEEVTNETYSSYMEKYIF 214 Query: 209 SPLDMKNSYTSTPEAKKHGLATGYKSIFGF----MAPFEQPDNPSMLASAYLISSAEDMS 264 PL+M + S A K G TGY+S FG + ++ P Y+ ++ EDM Sbjct: 215 QPLNMNGAAASKETAYKKGCLTGYQSWFGIPRKSVVSYDNAGAP----YGYITANLEDMI 270 Query: 265 HYLVAQLNGGKFHDISVAATESVAQMHQPAIKDPATGGEYGMGWEIVH-----GVVQHAG 319 Y++ LN + D + E++ P K + YG G + ++ H+G Sbjct: 271 QYIMF-LN--RPEDAQLLKQENMHLYLSPLYKINSEKS-YGFGLRTTNINESETMIWHSG 326 Query: 320 DVESFHADMKLDGDIGVV-VLMNAHDYAVRAFKLGMVADGILAIMHGREPVDDAGSITGT 378 A++ + G V++ ++ + L ++ GI++I++G EPVD I T Sbjct: 327 STPDARAELFTLNESGWSGVILTNKNHVLEETALSVLKKGIISILNGEEPVDIPKKIPLT 386 Query: 379 YILIDVISAAVMIMLGVSVFNLIKRKKSFRHTPLRIILFAFCLVLFNAVLPIAVLYGFSH 438 I++ V++ +I+ +S+ LIK+ K + I LF L L +++ I +L +S Sbjct: 387 QIILLVVT---LILFIISIM-LIKKYKHMKTVKKVIWLFIGSLSLLLSIIFIPLL-TYS- 440 Query: 439 TFAPWSVVFSFLPGLGHLAFVLSILSLVIGAAKLIVLMHNLR 480 T +PW + F P +A SI ++ LI ++ LR Sbjct: 441 TSSPWRTIKIFAPD---VALFTSISVTLLAVNGLISILIALR 479 >B7JPT0.1 B7JPT0_BACC0 Putative penicillin-binding protein Length = 463 Score = 216 bits (550), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 145/460 (31%), Positives = 246/460 (53%), Gaps = 30/460 (6%) Query: 34 IDTFVESMLEKLDFPGMAVGIVKGDQIIYTQGYGHSGPDGQPVTPQTPFILGSSSKSFTA 93 +D ++E +++ + PG AV IV ++ +T+ G +G + VT +TPF +GS SKS TA Sbjct: 19 LDKYIEKFIKEQNIPGAAVAIVHNKEVFFTKTMGVTGESEKKVTSKTPFAIGSISKSLTA 78 Query: 94 LAIMQLVEQGKIDLNAPVQRYLPEFELEDKDASKTILVRHLLNHSSGISTFHG---RTAF 150 LAI++L+E KI L PVQRYLP F+L++ S TI ++HLL H+SGI+T+ G Sbjct: 79 LAIVKLIEDKKIKLEDPVQRYLPWFKLKNPQISSTITIQHLLTHTSGINTYEGLALSDKQ 138 Query: 151 TNTASTIDELIHGLKNTPLTEPVGSQYQYSNANYDLLGGIIQAVSGESYAEYIQDHVYSP 210 + +++ + E + L N +T P G +YQYSNANY +LG +I+ V+ E+Y+ Y++ +++ P Sbjct: 139 SKSSTALKENVMKLSNVKVTAPPGEKYQYSNANYIVLGALIEEVTNETYSSYMEKYIFQP 198 Query: 211 LDMKNSYTSTPEAKKHGLATGYKSIFGF----MAPFEQPDNPSMLASAYLISSAEDMSHY 266 L+M + S A K G TGY+S FG + ++ P Y+ ++ EDM Y Sbjct: 199 LNMNGAAASKETAYKKGYLTGYQSWFGIPRKSVVSYDNAGAP----YGYITANLEDMIQY 254 Query: 267 LVAQLNGGKFHDISVAATESVAQMHQPAIKDPATGGEYGMGWEIVH-----GVVQHAGDV 321 ++ LN + D + E++ P K + YG G + ++ H+G Sbjct: 255 IMF-LN--RPEDAQLLKQENMHLYLSPLYKINSEKS-YGFGLRTTNINESETMIWHSGST 310 Query: 322 ESFHADMKLDGDIGVV-VLMNAHDYAVRAFKLGMVADGILAIMHGREPVDDAGSITGTYI 380 A++ + G V++ ++ + L ++ GI++I++G EPVD I T I Sbjct: 311 PDARAELFTLNESGWSGVILTNKNHVLEETALSVLKKGIISILNGEEPVDIPKKIPLTQI 370 Query: 381 LIDVISAAVMIMLGVSVFNLIKRKKSFRHTPLRIILFAFCLVLFNAVLPIAVLYGFSHTF 440 ++ V++ +I+ +S+ LIK+ K + I LF L L +++ I +L +S T Sbjct: 371 ILLVVT---LILFIISIM-LIKKYKHMKTVKKVIWLFIGSLSLLLSIIFIPLL-TYS-TS 424 Query: 441 APWSVVFSFLPGLGHLAFVLSILSLVIGAAKLIVLMHNLR 480 +PW + F P +A SI ++ LI ++ LR Sbjct: 425 SPWRTIKIFAPD---VALFTSISVTLLAVNGLISILIALR 461 >C3G3D3.1 C3G3D3_BACTU Putative uncharacterized protein Length = 463 Score = 216 bits (550), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 145/460 (31%), Positives = 246/460 (53%), Gaps = 30/460 (6%) Query: 34 IDTFVESMLEKLDFPGMAVGIVKGDQIIYTQGYGHSGPDGQPVTPQTPFILGSSSKSFTA 93 +D ++E +++ + PG AV IV ++ +T+ G +G + VT +TPF +GS SKS TA Sbjct: 19 LDKYIEKFIKEQNIPGAAVAIVHNKEVFFTKTMGVTGESEKKVTSKTPFAIGSISKSLTA 78 Query: 94 LAIMQLVEQGKIDLNAPVQRYLPEFELEDKDASKTILVRHLLNHSSGISTFHG---RTAF 150 LAI++L+E KI L PVQRYLP F+L++ S TI ++HLL H+SGI+T+ G Sbjct: 79 LAIVKLIEDKKIKLEDPVQRYLPWFKLKNPQISSTITIQHLLTHTSGINTYEGLALSDKQ 138 Query: 151 TNTASTIDELIHGLKNTPLTEPVGSQYQYSNANYDLLGGIIQAVSGESYAEYIQDHVYSP 210 + +++ + E + L N +T P G +YQYSNANY +LG +I+ V+ E+Y+ Y++ +++ P Sbjct: 139 SKSSTALKENVMKLSNVKVTAPPGEKYQYSNANYIVLGALIEEVTNETYSSYMEKYIFQP 198 Query: 211 LDMKNSYTSTPEAKKHGLATGYKSIFGF----MAPFEQPDNPSMLASAYLISSAEDMSHY 266 L+M + S A K G TGY+S FG + ++ P Y+ ++ EDM Y Sbjct: 199 LNMNGAAASKETAYKKGYLTGYQSWFGIPRKSVVSYDNAGAP----YGYITANLEDMIQY 254 Query: 267 LVAQLNGGKFHDISVAATESVAQMHQPAIKDPATGGEYGMGWEIVH-----GVVQHAGDV 321 ++ LN + D + E++ P K + YG G + ++ H+G Sbjct: 255 IMF-LN--RPEDAQLLKQENMHLYLSPLYKINSEKS-YGFGLRTTNINESETMIWHSGST 310 Query: 322 ESFHADMKLDGDIGVV-VLMNAHDYAVRAFKLGMVADGILAIMHGREPVDDAGSITGTYI 380 A++ + G V++ ++ + L ++ GI++I++G EPVD I T I Sbjct: 311 PDARAELFTLNESGWSGVILTNKNHVLEETALSVLKKGIISILNGEEPVDIPKKIPLTQI 370 Query: 381 LIDVISAAVMIMLGVSVFNLIKRKKSFRHTPLRIILFAFCLVLFNAVLPIAVLYGFSHTF 440 ++ V++ +I+ +S+ LIK+ K + I LF L L +++ I +L +S T Sbjct: 371 ILLVVT---LILFIISIM-LIKKYKHMKTVKKVIWLFIGSLSLLLSIIFIPLL-TYS-TS 424 Query: 441 APWSVVFSFLPGLGHLAFVLSILSLVIGAAKLIVLMHNLR 480 +PW + F P +A SI ++ LI ++ LR Sbjct: 425 SPWRTIKIFAPD---VALFTSISVTLLAVNGLISILIALR 461 >C3PB44.1 C3PB44_BACAA Putative penicillin-binding protein Length = 463 Score = 215 bits (547), Expect = 9e-54, Method: Compositional matrix adjust. Identities = 144/460 (31%), Positives = 245/460 (53%), Gaps = 30/460 (6%) Query: 34 IDTFVESMLEKLDFPGMAVGIVKGDQIIYTQGYGHSGPDGQPVTPQTPFILGSSSKSFTA 93 +D ++E +++ + PG AV IV ++ +T+ G +G + VT +TPF +GS SKS TA Sbjct: 19 LDKYIEKFIKEQNIPGAAVAIVHNKEVFFTKTMGVTGESEKKVTSKTPFAIGSISKSLTA 78 Query: 94 LAIMQLVEQGKIDLNAPVQRYLPEFELEDKDASKTILVRHLLNHSSGISTFHG---RTAF 150 LAI++L+E KI L PVQRYLP F+L++ S TI ++HLL H+SGI+T+ G Sbjct: 79 LAIVKLIEDKKIKLEDPVQRYLPWFKLKNPQISSTITIQHLLTHTSGINTYEGLALSDKQ 138 Query: 151 TNTASTIDELIHGLKNTPLTEPVGSQYQYSNANYDLLGGIIQAVSGESYAEYIQDHVYSP 210 + +++ + E + L N +T P G +YQYSNANY +LG +I+ V+ E+Y+ Y++ +++ P Sbjct: 139 SKSSTALKENVMKLSNVKVTAPPGEKYQYSNANYIVLGALIEEVTNETYSSYMEKYIFQP 198 Query: 211 LDMKNSYTSTPEAKKHGLATGYKSIFGF----MAPFEQPDNPSMLASAYLISSAEDMSHY 266 L+M + S A + G TGY+S FG + ++ P Y+ ++ EDM Y Sbjct: 199 LNMNGAAASKENAYEKGYLTGYQSWFGIPRKSVVSYDNTGAP----YGYITANLEDMIQY 254 Query: 267 LVAQLNGGKFHDISVAATESVAQMHQPAIKDPATGGEYGMGWEIVH-----GVVQHAGDV 321 ++ LN + D + E++ P K + YG G + ++ H+G Sbjct: 255 IMF-LN--RPEDAQLLKQENIHLYLSPLYKINSEKS-YGFGLRTTNINESETMIWHSGST 310 Query: 322 ESFHADMKLDGDIGVV-VLMNAHDYAVRAFKLGMVADGILAIMHGREPVDDAGSITGTYI 380 A++ + G V++ ++ + L ++ GI++I++G EPVD I T I Sbjct: 311 PDARAELFTLNESGWSGVILTNKNHVLEETALSVLKKGIISILNGEEPVDIPKKIPLTQI 370 Query: 381 LIDVISAAVMIMLGVSVFNLIKRKKSFRHTPLRIILFAFCLVLFNAVLPIAVLYGFSHTF 440 ++ V++ +I+ +S+ LIK+ K + I LF L L +++ I L+ +S T Sbjct: 371 ILLVVT---LILFIISIM-LIKKYKHMKTVKKVIWLFIGSLSLLLSIIFIP-LFTYS-TS 424 Query: 441 APWSVVFSFLPGLGHLAFVLSILSLVIGAAKLIVLMHNLR 480 +PW + F P +A SI ++ LI + LR Sbjct: 425 SPWRTIKIFAPD---VALFTSISVTLLAVNGLISIFIALR 461 >Q81Q59.1 Q81Q59_BACAN Penicillin-binding protein, putative Length = 481 Score = 215 bits (547), Expect = 9e-54, Method: Compositional matrix adjust. Identities = 137/434 (31%), Positives = 235/434 (54%), Gaps = 27/434 (6%) Query: 34 IDTFVESMLEKLDFPGMAVGIVKGDQIIYTQGYGHSGPDGQPVTPQTPFILGSSSKSFTA 93 +D ++E +++ + PG AV IV ++ +T+ G +G + VT +TPF +GS SKS TA Sbjct: 37 LDKYIEKFIKEQNIPGAAVAIVHNKEVFFTKTMGVTGESEKKVTSKTPFAIGSISKSLTA 96 Query: 94 LAIMQLVEQGKIDLNAPVQRYLPEFELEDKDASKTILVRHLLNHSSGISTFHG---RTAF 150 LAI++L+E KI L PVQRYLP F+L++ S TI ++HLL H+SGI+T+ G Sbjct: 97 LAIVKLIEDKKIKLEDPVQRYLPWFKLKNPQISSTITIQHLLTHTSGINTYEGLALSDKQ 156 Query: 151 TNTASTIDELIHGLKNTPLTEPVGSQYQYSNANYDLLGGIIQAVSGESYAEYIQDHVYSP 210 + +++ + E + L N +T P G +YQYSNANY +LG +I+ V+ E+Y+ Y++ +++ P Sbjct: 157 SKSSTALKENVMKLSNVKVTAPPGEKYQYSNANYIVLGALIEEVTNETYSSYMEKYIFQP 216 Query: 211 LDMKNSYTSTPEAKKHGLATGYKSIFGF----MAPFEQPDNPSMLASAYLISSAEDMSHY 266 L+M + S A + G TGY+S FG + ++ P Y+ ++ EDM Y Sbjct: 217 LNMNGAAASKENAYEKGYLTGYQSWFGIPRKSVVSYDNTGAP----YGYITANLEDMIQY 272 Query: 267 LVAQLNGGKFHDISVAATESVAQMHQPAIKDPATGGEYGMGWEIVH-----GVVQHAGDV 321 ++ LN + D + E++ P K + YG G + ++ H+G Sbjct: 273 IMF-LN--RPEDAQLLKQENIHLYLSPLYKINSEKS-YGFGLRTTNINESETMIWHSGST 328 Query: 322 ESFHADMKLDGDIGVV-VLMNAHDYAVRAFKLGMVADGILAIMHGREPVDDAGSITGTYI 380 A++ + G V++ ++ + L ++ GI++I++G EPVD I T I Sbjct: 329 PDARAELFTLNESGWSGVILTNKNHVLEETALSVLKKGIISILNGEEPVDIPKKIPLTQI 388 Query: 381 LIDVISAAVMIMLGVSVFNLIKRKKSFRHTPLRIILFAFCLVLFNAVLPIAVLYGFSHTF 440 ++ V++ +I+ +S+ LIK+ K + I LF L L +++ I L+ +S T Sbjct: 389 ILLVVT---LILFIISIM-LIKKYKHMKTVKKVIWLFIGSLSLLLSIIFIP-LFTYS-TS 442 Query: 441 APWSVVFSFLPGLG 454 +PW + F P + Sbjct: 443 SPWRTIKIFAPDVA 456 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,943,492,175 Number of extensions: 126538124 Number of successful extensions: 344238 Number of sequences better than 1.0e-05: 4002 Number of HSP's gapped: 338583 Number of HSP's successfully gapped: 4013 Length of query: 491 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 351 Effective length of database: 1,741,754,103 Effective search space: 611355690153 Effective search space used: 611355690153 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)