BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0172.0010.p (432 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG56.1 B3KG56_9BACI Thiamine biosynthesis/tRNA modification pr... 558 e-157 B1DBV0.1 B1DBV0_9BACL Thiamine biosynthesis/tRNA modification pr... 429 e-118 C0ZH32.1 THII_BREBN Probable thiamine biosynthesis protein thiI 376 e-102 C0GIE1.1 C0GIE1_9FIRM Thiamine biosynthesis/tRNA modification pr... 375 e-102 C3IXL4.1 C3IXL4_9BACI Thiamine biosynthesis/tRNA modification pr... 373 e-101 >B3KG56.1 B3KG56_9BACI Thiamine biosynthesis/tRNA modification protein ThiI Length = 434 Score = 558 bits (1438), Expect = e-157, Method: Compositional matrix adjust. Identities = 267/388 (68%), Positives = 321/388 (82%) Query: 24 DMLLLRFGEFTLKGKNRSRFEKTVLRHVKEMIRPFPKVTLSKEFGRIYVQLNGEPAEELA 83 D LLLRFGEFTLKGKNR+RFE++VL H++ ++RP+P + KE+GR+YV+LNGE E L Sbjct: 4 DKLLLRFGEFTLKGKNRNRFERSVLNHIRSVLRPYPGAKIIKEYGRLYVELNGESPEALI 63 Query: 84 DVLRNVFGIASISPVKVSLSDFDDILSVSRNFLRIAAPPTGTTFKVNVRRVWKGFPYGSI 143 VL+ VFG+ SISPV+V S+ D I+ + F+ TFKVN RRVWK FP+ S Sbjct: 64 QVLKKVFGLVSISPVRVCSSEPDRIIETAVEFVGQKELEGTVTFKVNARRVWKQFPHSSQ 123 Query: 144 EMNKLISTPLLQSYPGLLVDVKSPQMELRIEIREGHTYIFSENIAGVGGFPLGTNGKAML 203 EMN L+ +PLL++YP L V VK P MELR+EIRE TY++ E I G GG+PLGTNGKAML Sbjct: 124 EMNHLVGSPLLRTYPQLKVKVKQPDMELRVEIRENETYLYYEVIPGAGGYPLGTNGKAML 183 Query: 204 LLSGGIDSPVAGWSSMRRGLEVECVHFYSFPYTSELARQKVVDLTRVLSRYAGVIKLHLV 263 LLSGGIDSPVAGWS+MRRGLE+ECVHFYSFPYTS+LA++KV++LTR+LS Y G IKLHLV Sbjct: 184 LLSGGIDSPVAGWSAMRRGLEIECVHFYSFPYTSQLAKEKVMELTRLLSGYTGKIKLHLV 243 Query: 264 PFTEVQTSFTGIGQDNLIITLMRRAMLKIATRLAEREGALALVTGESLGQVASQTLSSMN 323 PFT+VQTSF GIGQDNLIIT MRRAML+IA+ LAEREGALA+VTG+SLGQVASQTL SMN Sbjct: 244 PFTDVQTSFVGIGQDNLIITFMRRAMLRIASSLAEREGALAIVTGDSLGQVASQTLPSMN 303 Query: 324 VIGRATPLPLLRPLVMMDKSEIVELSKTVGTYDLSILPYEDCCTLFVPKSPTTNPNLRIV 383 VIGRAT LPLLRPLVMMDK++I+ ++ +GT+D SILPYEDCCTLFVPKSPTTNPNL+I+ Sbjct: 304 VIGRATELPLLRPLVMMDKNDIINIAMDIGTFDTSILPYEDCCTLFVPKSPTTNPNLKII 363 Query: 384 DKIEATLPGYSALLDAAVAGTETVNITP 411 +K+EA+L AL+ AAV GTETV ITP Sbjct: 364 EKVEASLTELPALIQAAVEGTETVIITP 391 >B1DBV0.1 B1DBV0_9BACL Thiamine biosynthesis/tRNA modification protein ThiI Length = 406 Score = 429 bits (1104), Expect = e-118, Method: Compositional matrix adjust. Identities = 210/410 (51%), Positives = 295/410 (71%), Gaps = 8/410 (1%) Query: 24 DMLLLRFGEFTLKGKNRSRFEKTVLRHVKEMIRPFPKVTLSKEFGRIYVQLNGEPAEELA 83 D +++R+G+ +KG+NR++FEK + + +K ++ F +T +K FGR+ + LNGE E +A Sbjct: 4 DKVIVRYGDLVMKGRNRNQFEKRMQQQIKLALKSFAALTYAKSFGRMTISLNGESFEPIA 63 Query: 84 DVLRNVFGIASISPVKVSLSDFDDILSVSRNFLRIAAPPTGTTFKVNVRRVWKGFPYGSI 143 + L++VFGI S SPV ++ + I + + + TFKVN +R WK F + S Sbjct: 64 ERLKDVFGIISFSPVIQVENELEKIQAGALQLM-TGLEKEPATFKVNAKRAWKPFKHSSQ 122 Query: 144 EMNKLISTPLLQSYPGLLVDVKSPQMELRIEIREGHTYIFSENIAGVGGFPLGTNGKAML 203 EMN L+ + +L+++ L VDV+ P++ELR++ E T+I++ I G GGFP G+NGKAML Sbjct: 123 EMNHLVGSHVLRAFADLKVDVRKPEVELRVDTYEEGTFIYNTVIPGAGGFPFGSNGKAML 182 Query: 204 LLSGGIDSPVAGWSSMRRGLEVECVHFYSFPYTSELARQKVVDLTRVLSRYAGV-IKLHL 262 LLSGGIDSPVAGW +MR+GLE+E VHF+S+P+TS+ A++KV+DL R LS YAG +KLHL Sbjct: 183 LLSGGIDSPVAGWMAMRKGLELEAVHFHSYPFTSDKAKEKVIDLARRLSYYAGAPLKLHL 242 Query: 263 VPFTEVQTSFTGIGQDNLIITLMRRAMLKIATRLAEREGALALVTGESLGQVASQTLSSM 322 VPFT++QT QD+++ITLMRRAML+I +LA+R GA +VTGESLGQVASQTLSSM Sbjct: 243 VPFTDIQTRLVQAKQDSMMITLMRRAMLRITEQLADRNGAGGIVTGESLGQVASQTLSSM 302 Query: 323 NVIGRATPLPLLRPLVMMDKSEIVELSKTVGTYDLSILPYEDCCTLFVPKSPTTNPNLRI 382 NVIGRAT LPLL+PL+MMDK EI+ + +GT++ SILPYEDCCTLF+PKSP+TNPNL + Sbjct: 303 NVIGRATVLPLLKPLIMMDKQEIINEAVRIGTFETSILPYEDCCTLFLPKSPSTNPNLNV 362 Query: 383 VDKIEATLPGYSALLDAAVAGTETVNITPYGDEKPSDVVPAQAGLQEEWF 432 V++ E+ +P +L AV TE + +TP E + PA ++ WF Sbjct: 363 VERAESYIPELVQMLLEAVERTEVIELTP---EAQPEQEPAG---EDRWF 406 >C0ZH32.1 THII_BREBN Probable thiamine biosynthesis protein thiI Length = 399 Score = 376 bits (966), Expect = e-102, Method: Compositional matrix adjust. Identities = 185/397 (46%), Positives = 270/397 (68%), Gaps = 2/397 (0%) Query: 24 DMLLLRFGEFTLKGKNRSRFEKTVLRHVKEMIRPFPKVTLSKEFGRIYVQLNGEPAEELA 83 D++L+R+GE LKGKNR +FE+ +++ V+ ++R F KV + + +GR+YV+L+GE A + Sbjct: 4 DVILIRYGELALKGKNRDQFEEALVKSVRNVLRSFFKVKVRRNYGRMYVELHGEDAYAVM 63 Query: 84 DVLRNVFGIASISPVKVSLSDFDDILSVSRNFLRIAAPPTGTTFKVNVRRVWKGFPYGSI 143 + L+ VFGI+S SP D + I + +R P TF+V RR K +P S+ Sbjct: 64 ERLKRVFGISSFSPTIQVDPDIETIKEKALELVR-QLNPQPRTFRVVSRRADKRYPIQSM 122 Query: 144 EMNKLISTPLLQSYPGLLVDVKSPQMELRIEIREGHTYIFSENIAGVGGFPLGTNGKAML 203 E+N++++T +L++ P + VD+ P + +EIR TYI E I G+GG P+G +GK +L Sbjct: 123 EVNRMVATHILRALPAISVDLHEPDTIVNVEIRTEGTYISCETIKGLGGLPVGVSGKVLL 182 Query: 204 LLSGGIDSPVAGWSSMRRGLEVECVHFYSFPYTSELARQKVVDLTRVLSRYAGVIKLHLV 263 LLSGGIDSPVAGW ++RG+ +E +HF+S+P+TSE A QKV DL L+++ G ++LH+V Sbjct: 183 LLSGGIDSPVAGWMMLKRGVTLEAIHFHSYPFTSERALQKVRDLAHKLTKWGGTVRLHVV 242 Query: 264 PFTEVQTSFTGIGQDNLIITLMRRAMLKIATRLAEREGALALVTGESLGQVASQTLSSMN 323 PFTE+QT+ ++ +IT+MRR M++I+ R+AE A AL TGESLGQVASQTL SM+ Sbjct: 243 PFTEIQTAIREKCPEDYLITIMRRFMMRISERVAENTNAKALATGESLGQVASQTLESMD 302 Query: 324 VIGRATPLPLLRPLVMMDKSEIVELSKTVGTYDLSILPYEDCCTLFVPKSPTTNPNLRIV 383 I + +P+LRPLV MDK +IV++S+ + TY+LSILPYEDCCT+F PK+P T P R+ Sbjct: 303 TINKVISIPILRPLVAMDKVDIVDISRQIDTYELSILPYEDCCTVFTPKNPVTRPKPRLA 362 Query: 384 DKIEATLPGYSALLDAAVAGTETVNITPYGDEKPSDV 420 K E L AL++ AVA TE IT E +D+ Sbjct: 363 AKFEEVL-DVEALVEDAVARTEIEEITTKPKETTTDL 398 >C0GIE1.1 C0GIE1_9FIRM Thiamine biosynthesis/tRNA modification protein ThiI Length = 390 Score = 375 bits (964), Expect = e-102, Method: Compositional matrix adjust. Identities = 187/387 (48%), Positives = 268/387 (69%), Gaps = 4/387 (1%) Query: 25 MLLLRFGEFTLKGKNRSRFEKTVLRHVKEMIRPFPKVTLSKEFGRIYVQLNGEPAEELAD 84 + L+R+GE LKGKNR FE T+ +++K + + + + R +V++ AE +A Sbjct: 4 LFLVRYGEIGLKGKNRKFFENTLTKNIKRALLDVADCRIHQTYSRNFVEVVDGDAEAVAA 63 Query: 85 VLRNVFGIASISPVKVSLSDFDDILSVSR-NFLRIAAPPTGTTFKVNVRRVWKGFPYGSI 143 L VFGI SISPV ++ D D+I +V++ F +I P G TFKV +R K FPY S Sbjct: 64 RLSQVFGIVSISPVAIAPLDLDEIKAVAKAEFEKITRP--GVTFKVETKRANKRFPYKSP 121 Query: 144 EMNKLISTPLLQSYPGLLVDVKSPQMELRIEIREGHTYIFSENIAGVGGFPLGTNGKAML 203 E+++ + LL + P L VDV++P+++L +EIRE TYI++ IAG GG P+G GK +L Sbjct: 122 EVSREVGAHLLINIPDLKVDVRNPEIKLEVEIREHETYIYTRTIAGPGGLPVGVAGKGLL 181 Query: 204 LLSGGIDSPVAGWSSMRRGLEVECVHFYSFPYTSELARQKVVDLTRVLSRYAGVIKLHLV 263 L+SGGIDSPVAGW SM+RG+ +E +HF+SFP+TS+ A++KV+DL R L+R+ G I+LH+ Sbjct: 182 LISGGIDSPVAGWMSMKRGVTLEAIHFHSFPFTSDRAKEKVIDLCRELTRFGGKIRLHVA 241 Query: 264 PFTEVQTSFTGIGQDNLIITLMRRAMLKIATRLAEREGALALVTGESLGQVASQTLSSMN 323 FTE+Q + L +TLMRR M +IA RLAE+ GALAL+TGESLGQVASQT+ S+N Sbjct: 242 HFTEIQKELRLKTPERLTVTLMRRMMFRIAERLAEQRGALALITGESLGQVASQTMESIN 301 Query: 324 VIGRATPLPLLRPLVMMDKSEIVELSKTVGTYDLSILPYEDCCTLFVPKSPTTNPNLRIV 383 VI + T +P+ RPL+ +DK EI+ +S+ + TY +SI PYEDCCT+F+P P T P L V Sbjct: 302 VIEKVTTIPVFRPLIGLDKEEIMNISRRIDTYPISIRPYEDCCTVFLPDFPATRPRLADV 361 Query: 384 DKIEATLPGYSALLDAAVAGTETVNIT 410 ++ EA+L AL+ ++A TET++IT Sbjct: 362 EEAEASL-DIEALISESLAKTETLDIT 387 >C3IXL4.1 C3IXL4_9BACI Thiamine biosynthesis/tRNA modification protein ThiI Length = 402 Score = 373 bits (958), Expect = e-101, Method: Compositional matrix adjust. Identities = 199/396 (50%), Positives = 267/396 (67%), Gaps = 7/396 (1%) Query: 24 DMLLLRFGEFTLKGKNRSRFEKTVLRHVKEMIRPFPKVTLSKEFGRIYVQLNGEPAEELA 83 D +L+R+GE T KGKNR+ F + + ++ ++ F ++ + R+Y+ LNGEP E + Sbjct: 5 DRILIRYGEMTTKGKNRNIFVRRLKSNIARKLQAFERIQIEYMRDRMYILLNGEPHEPII 64 Query: 84 DVLRNVFGIASISPVKVSLSDFDDILSVSRNFLRIAAPPTGTTFKVNVRRVWKGFPYGSI 143 + L+ VFGI S S +D D I + +R P G TFKV+ RRV K FPY S Sbjct: 65 EKLKTVFGIHSFSLAMKCENDLDAIKETALAAVR-QLPYKGKTFKVSARRVDKQFPYRSD 123 Query: 144 EMNKLISTPLLQSYPGLLVDVKSPQMELRIEIREGHTYIFSENIAGVGGFPLGTNGKAML 203 E+N + +L+ GL V+V+ P +++RIE+R+ TYI +I G GG P+GT+GKAML Sbjct: 124 ELNYEVGAHILRQTDGLRVNVREPDIDVRIEVRQDGTYITCRDIFGAGGLPVGTSGKAML 183 Query: 204 LLSGGIDSPVAGWSSMRRGLEVECVHFYSFPYTSELARQKVVDLTRVLSRYAGVIKLHLV 263 +LSGGIDSPVAG+ SM+RGLE+E VHF+S P+TSE A+QKV+DL R L+ Y G IKLH+V Sbjct: 184 MLSGGIDSPVAGYLSMKRGLEIEAVHFFSPPFTSERAKQKVIDLVRKLTTYGGRIKLHIV 243 Query: 264 PFTEVQTS-FTGIGQDNLIITLMRRAMLKIATRLAEREGALALVTGESLGQVASQTLSSM 322 PFTEVQ + + G+ + +I+ RRAML+I L R+ ALA+VTGESLGQVASQTL SM Sbjct: 244 PFTEVQQAIYQGVPNEYSLIS-TRRAMLRITDALRRRQRALAIVTGESLGQVASQTLESM 302 Query: 323 NVIGRATPLPLLRPLVMMDKSEIVELSKTVGTYDLSILPYEDCCTLFVPKSPTTNPNLRI 382 VI T P+LRPLV MDK EI+EL+K +GT+D+SILPYEDCCT+F P++P T P Sbjct: 303 YVINEVTNTPILRPLVSMDKLEIIELAKQIGTHDISILPYEDCCTIFTPRAPKTKPKKEK 362 Query: 383 VDKIEATLPGYSALLDAAVAGTETVNI---TPYGDE 415 V E+ L + LL+ AV TET+ I T GDE Sbjct: 363 VLHYESQL-DLAPLLEKAVNDTETLVIDEETGQGDE 397 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,533,679,659 Number of extensions: 105361448 Number of successful extensions: 248645 Number of sequences better than 1.0e-05: 786 Number of HSP's gapped: 247444 Number of HSP's successfully gapped: 786 Length of query: 432 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 293 Effective length of database: 1,751,175,118 Effective search space: 513094309574 Effective search space used: 513094309574 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)