BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0024.p (289 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KDK8.1 B3KDK8_9BACI Cation diffusion facilitator family transp... 380 e-104 B1D5R9.1 B1D5R9_9BACL Cation diffusion facilitator family transp... 324 6e-87 C0ZFR0.1 C0ZFR0_BREBN Conserved hypothetical membrane protein 278 4e-73 P46348.2 YEAB_BACSU Uncharacterized transporter yeaB 270 1e-70 A8FAH1.1 A8FAH1_BACP2 CDF family cation diffusion facilitator 269 2e-70 >B3KDK8.1 B3KDK8_9BACI Cation diffusion facilitator family transporter Length = 290 Score = 380 bits (977), Expect = e-104, Method: Compositional matrix adjust. Identities = 183/284 (64%), Positives = 216/284 (76%) Query: 5 YDDIRKGERGXXXXXXXXXXXXXFKLISGYVFGSSALLADGFNNLTDIVASLAVLIGLRI 64 YD+I++GERG FK+ GY+F SSALLADGFNNLTDIVASLAVLIGLRI Sbjct: 4 YDNIKQGERGAWISIAAYLVLSSFKIFCGYLFASSALLADGFNNLTDIVASLAVLIGLRI 63 Query: 65 SRKPPDSDHTYGHFRAETVAALLASFIMAMVGIQVIVEAVRSLFEGAKETPQLWSXXXXX 124 S+KPPDSDH YGH RAET+AAL+ASFIMA+VGIQV+VEAVRS FEG+KE P LWS Sbjct: 64 SQKPPDSDHAYGHLRAETIAALVASFIMAVVGIQVLVEAVRSFFEGSKEVPNLWSAGVAG 123 Query: 125 XXXXXXXXXYIYNKRLARQINNGALLAAAKDNFSDAMVSIGAAVGIVGAQFGLPWIDSAA 184 Y YN+ LAR+I+N AL+AAAKDN SDA+VS+GAAVGI+GAQFGLPW+D+ A Sbjct: 124 ICAVAMLGVYRYNRNLARRIDNQALMAAAKDNLSDALVSVGAAVGIIGAQFGLPWLDTVA 183 Query: 185 AVAVGLLILKTAWDIFRDSTYRLTDGFDQDRLLDLRSTIARTPGVEGIKDLKARXXXXXX 244 AVAVG++I KTAW+IFRD TY LTDGFD++RL DLRSTIARTPGVEGIKD+KAR Sbjct: 184 AVAVGVIICKTAWEIFRDCTYSLTDGFDENRLSDLRSTIARTPGVEGIKDMKARIHGNHV 243 Query: 245 XXXXXXXXXXXXXXMQGHEISDSIEERMSTLHNIMNVQVHVEPK 288 ++GH+ISD IEE+M +HNIM+V +HVEPK Sbjct: 244 LVDVVIEVDPDISVLEGHQISDRIEEQMEKIHNIMSVHIHVEPK 287 >B1D5R9.1 B1D5R9_9BACL Cation diffusion facilitator family transporter Length = 298 Score = 324 bits (830), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 161/285 (56%), Positives = 195/285 (68%) Query: 5 YDDIRKGERGXXXXXXXXXXXXXFKLISGYVFGSSALLADGFNNLTDIVASLAVLIGLRI 64 Y++I++GE+G KL +GY F S AL+ADGFNNLTDI+AS+AVL+GLRI Sbjct: 4 YEEIKQGEKGALVSIGAYIGLSAIKLGAGYWFASGALVADGFNNLTDIIASVAVLVGLRI 63 Query: 65 SRKPPDSDHTYGHFRAETVAALLASFIMAMVGIQVIVEAVRSLFEGAKETPQLWSXXXXX 124 S+KPPD DH YGHFRAET+AAL+ASFIMA VG+QVI+ VRSLF G + P L S Sbjct: 64 SQKPPDKDHPYGHFRAETIAALIASFIMATVGLQVIINTVRSLFAGGQTVPSLTSAWVAL 123 Query: 125 XXXXXXXXXYIYNKRLARQINNGALLAAAKDNFSDAMVSIGAAVGIVGAQFGLPWIDSAA 184 Y YN+RLA +INN AL AAAKDN SD +VSIGAAVGI+GAQFGLPW+D A Sbjct: 124 FAAACMGAVYFYNRRLANRINNQALHAAAKDNLSDTLVSIGAAVGIIGAQFGLPWLDPVA 183 Query: 185 AVAVGLLILKTAWDIFRDSTYRLTDGFDQDRLLDLRSTIARTPGVEGIKDLKARXXXXXX 244 A+AVG +I KTAWDIF ST+ LTDGFD + L+ LRSTI RT GV+ IKD+KAR Sbjct: 184 ALAVGAIICKTAWDIFYSSTHALTDGFDANELMTLRSTIERTKGVKSIKDIKARVHGSNV 243 Query: 245 XXXXXXXXXXXXXXMQGHEISDSIEERMSTLHNIMNVQVHVEPKQ 289 ++ H ISD IE++M HNIM+V VHVEP + Sbjct: 244 LIDVIVQVDPGLTLIESHRISDEIEQQMEGKHNIMSVHVHVEPHE 288 >C0ZFR0.1 C0ZFR0_BREBN Conserved hypothetical membrane protein Length = 293 Score = 278 bits (711), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 142/285 (49%), Positives = 180/285 (63%) Query: 3 DIYDDIRKGERGXXXXXXXXXXXXXFKLISGYVFGSSALLADGFNNLTDIVASLAVLIGL 62 D+Y D+++GERG K+ YV S AL+ADG NN TD+VAS+AVLIGL Sbjct: 2 DVYSDLKQGERGAWVSIFAYIFCSVLKIGVAYVTASEALMADGLNNSTDVVASIAVLIGL 61 Query: 63 RISRKPPDSDHTYGHFRAETVAALLASFIMAMVGIQVIVEAVRSLFEGAKETPQLWSXXX 122 RI+RKPPD+DH YGHFRAET++AL+ASFIM VG+QVI AV SLF+ P L + Sbjct: 62 RIARKPPDNDHPYGHFRAETISALVASFIMLFVGVQVITNAVPSLFDPKNSAPDLLAGWT 121 Query: 123 XXXXXXXXXXXYIYNKRLARQINNGALLAAAKDNFSDAMVSIGAAVGIVGAQFGLPWIDS 182 Y YNK+LA + N+ AL AAA DN SDA VSIG +G+ GAQF L W+D Sbjct: 122 ALATAVIMLFVYRYNKKLAIKTNSQALDAAAADNRSDAFVSIGTFIGVAGAQFQLHWLDP 181 Query: 183 AAAVAVGLLILKTAWDIFRDSTYRLTDGFDQDRLLDLRSTIARTPGVEGIKDLKARXXXX 242 AA VGL+ILKTAWDIFR++T+RLTDGFD+ L DLR+TI+ GV I D+KAR Sbjct: 182 LAAFVVGLIILKTAWDIFREATHRLTDGFDESDLADLRATISSISGVRDISDIKARYHGS 241 Query: 243 XXXXXXXXXXXXXXXXMQGHEISDSIEERMSTLHNIMNVQVHVEP 287 ++ H I++ IEE+M +H I V +H+EP Sbjct: 242 SVLVDVVIHVDPGLNVVESHTITEKIEEQMRKIHRINAVHIHIEP 286 >P46348.2 YEAB_BACSU Uncharacterized transporter yeaB Length = 290 Score = 270 bits (691), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 137/283 (48%), Positives = 175/283 (61%) Query: 5 YDDIRKGERGXXXXXXXXXXXXXFKLISGYVFGSSALLADGFNNLTDIVASLAVLIGLRI 64 YD+++KGE G KLI GY+F S AL ADG NN TDI+AS+AVLIGLRI Sbjct: 4 YDELKKGESGALVSIAAYLVLSAIKLIIGYLFHSEALTADGLNNTTDIIASVAVLIGLRI 63 Query: 65 SRKPPDSDHTYGHFRAETVAALLASFIMAMVGIQVIVEAVRSLFEGAKETPQLWSXXXXX 124 S+KPPD DH YGHFRAET+A+L+ASFIM +VG+QV+ A S+F +ETP + + Sbjct: 64 SQKPPDEDHPYGHFRAETIASLIASFIMMVVGLQVLFSAGESIFSAKQETPDMIAAWTAA 123 Query: 125 XXXXXXXXXYIYNKRLARQINNGALLAAAKDNFSDAMVSIGAAVGIVGAQFGLPWIDSAA 184 Y YNKRLA+++ + ALLAAA DN SDA VSIG +GIV AQF L WID+ Sbjct: 124 GGAVLMLIVYRYNKRLAKKVKSQALLAAAADNKSDAFVSIGTFIGIVAAQFHLAWIDTVT 183 Query: 185 AVAVGLLILKTAWDIFRDSTYRLTDGFDQDRLLDLRSTIARTPGVEGIKDLKARXXXXXX 244 A +GLLI KTAWDIF++S++ LTDGFD + + TI + GV +KD+KAR Sbjct: 184 AFVIGLLICKTAWDIFKESSHSLTDGFDIKDISAYKQTIEKISGVSRLKDIKARYLGSTV 243 Query: 245 XXXXXXXXXXXXXXMQGHEISDSIEERMSTLHNIMNVQVHVEP 287 + H+I++ IE RM H I VH+EP Sbjct: 244 HVDVVVEVSADLNITESHDIANEIERRMKEEHAIDYSHVHMEP 286 >A8FAH1.1 A8FAH1_BACP2 CDF family cation diffusion facilitator Length = 299 Score = 269 bits (687), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 135/283 (47%), Positives = 178/283 (62%) Query: 5 YDDIRKGERGXXXXXXXXXXXXXFKLISGYVFGSSALLADGFNNLTDIVASLAVLIGLRI 64 Y+++R+GE G KL+ GY F S AL ADG NN TDI+ASLAVLIGLRI Sbjct: 4 YNELRQGETGAWVSIIAYVILSAVKLLIGYTFHSEALSADGLNNTTDIIASLAVLIGLRI 63 Query: 65 SRKPPDSDHTYGHFRAETVAALLASFIMAMVGIQVIVEAVRSLFEGAKETPQLWSXXXXX 124 S+KPPD DH YGHFRAE +A+L+ASFIM +VG+QV++ A +SLF +TP + + Sbjct: 64 SQKPPDEDHPYGHFRAENIASLVASFIMMLVGLQVLLSAGQSLFSSEHQTPDMIAAWTAA 123 Query: 125 XXXXXXXXXYIYNKRLARQINNGALLAAAKDNFSDAMVSIGAAVGIVGAQFGLPWIDSAA 184 YIYN+ L+++IN+ AL AAA DN SDA VSIG VGI+ +QF L WID+ A Sbjct: 124 GSAVVMYGVYIYNRNLSKRINSQALHAAAADNKSDAYVSIGTFVGIIASQFQLAWIDTLA 183 Query: 185 AVAVGLLILKTAWDIFRDSTYRLTDGFDQDRLLDLRSTIARTPGVEGIKDLKARXXXXXX 244 A +GL+I KTAW+IFRD+++ LTDGF + + TI TPGV +KD+KAR Sbjct: 184 AFVIGLIICKTAWEIFRDASHSLTDGFHIKDMSKYKETIEATPGVGDLKDIKARYLGSTI 243 Query: 245 XXXXXXXXXXXXXXMQGHEISDSIEERMSTLHNIMNVQVHVEP 287 + H+I+D IE RM H+I++ VH+EP Sbjct: 244 HVDVVVEVEPHLNIAESHDIADEIERRMKKEHDILHSHVHMEP 286 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,322,833,040 Number of extensions: 43011312 Number of successful extensions: 116703 Number of sequences better than 1.0e-05: 2067 Number of HSP's gapped: 113932 Number of HSP's successfully gapped: 2071 Length of query: 289 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 155 Effective length of database: 1,798,280,193 Effective search space: 278733429915 Effective search space used: 278733429915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)