BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0008.p (585 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q08341.1 CDAS_BACSH Cyclomaltodextrinase EC=3.2.1.54 874 0.0 B1D8P8.1 B1D8P8_9BACL Alpha amylase catalytic region 703 0.0 Q84AP9.1 Q84AP9_9BACL Cyclodextrinase enzyme 682 0.0 B3K4I1.1 B3K4I1_9BACI Alpha amylase catalytic region 676 0.0 Q59226.1 Q59226_BACSP Cyclomaltodextrinase EC=3.2.1.54 675 0.0 >Q08341.1 CDAS_BACSH Cyclomaltodextrinase EC=3.2.1.54 Length = 591 Score = 874 bits (2257), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/583 (69%), Positives = 485/583 (83%), Gaps = 4/583 (0%) Query: 3 MIHLESVYHRMGQNWSYAYDETVIHIRLRTKRDDVSRVDLICGDKYGWDRTHSEYAMERL 62 MI LE+VYHRMGQNWSYAY+++ +HIR+RTKRD+V R+DL CG+KY ++ MER+ Sbjct: 1 MIMLEAVYHRMGQNWSYAYNDSTLHIRIRTKRDNVPRIDLHCGEKYDPEKYKETIPMERM 60 Query: 63 SSDNLFDYWQAAVRPPHRRLAYYFALHQD-GNTVYFLEKGFLEHPPEIIYEGLFDFPILS 121 +SD LFDYWQAAV+P +RRL YYFALH D G+ VYF+EKGF + PP+++YEGLFDFP L+ Sbjct: 61 ASDGLFDYWQAAVQPRYRRLVYYFALHSDNGDAVYFMEKGFFDQPPKVMYEGLFDFPYLN 120 Query: 122 PEDVHQPPAWVKDAVFYQIFPERFANGDPSNDPEGVLEWGGTPSPTNFFGGDLQGVLEHL 181 +DVH PPAWVK+A+FYQIFPERFANGDPSNDPEGV EWGGTPS NFFGGDLQGV++HL Sbjct: 121 RQDVHTPPAWVKEAIFYQIFPERFANGDPSNDPEGVQEWGGTPSAGNFFGGDLQGVIDHL 180 Query: 182 DYLCALGINAIYFNPLFAATTNHKYDTADYLRIDPHFGTNELLKELVDACHAKGIRVLLD 241 DYL LG+NA+YFNPLFAATTNHKYDTADY++IDP FGTNE LKELVDACHA+G+RVLLD Sbjct: 181 DYLSDLGVNALYFNPLFAATTNHKYDTADYMKIDPQFGTNEKLKELVDACHARGMRVLLD 240 Query: 242 GVFNHCGHTFPPFMDVLENGRASRYADWFHVKEWPPVVNGGIPTYDTFAFEPIMPKFNTA 301 VFNHCGHTFPPF+DVL NG SRYADWFHV+EWP V GIPTYDTFAFEPIMPK NT Sbjct: 241 AVFNHCGHTFPPFVDVLNNGLNSRYADWFHVREWPLRVVDGIPTYDTFAFEPIMPKLNTG 300 Query: 302 NPEVKEYLLKVGRYWIEEIGVDGWRLDVANEVSHQFWRDFRTMVKQINPDAYLVGEIMHD 361 N EVK YLL VGRYW+EE+G+DGWRLDVANEV HQFWR+FR+ +K+INP AY++GEIMHD Sbjct: 301 NEEVKAYLLNVGRYWLEEMGLDGWRLDVANEVDHQFWREFRSEIKRINPSAYILGEIMHD 360 Query: 362 SLPWLLGDQFDAVMNYQLTNMLLNFFARGQTSAGQFSRSVGTLLANYPQQITEVSFNLLD 421 S+PWL GDQFDAVMNY TN+LLNFFAR T+A +F++++GT LA YPQQ+TEVSFNLL Sbjct: 361 SMPWLQGDQFDAVMNYPFTNILLNFFARRLTNAAEFAQAIGTQLAGYPQQVTEVSFNLLG 420 Query: 422 SHDTVRFLTLCGGDIRRLKLAFLFLMTYQGTPCIYYGDEIGMDGEYDPHNRKCMEWEPRK 481 SHDT R LTLC G++ R+KLA LF +TYQGTPCIYYGDEIGMDGEYDP NRKCMEW+ K Sbjct: 421 SHDTTRLLTLCSGNVERMKLATLFQLTYQGTPCIYYGDEIGMDGEYDPLNRKCMEWDKSK 480 Query: 482 QNQDLLEYYRWAIRLRKANSAFRSSAIKFLEHPEHPELLVYERWDEHDRFLVIMNNSELP 541 QN +LL ++R I LRKA+ A R S ++FL EHP+LLVYERWD+++RFL+++NN + P Sbjct: 481 QNTELLAFFRSMISLRKAHPALRGSGLRFLPVLEHPQLLVYERWDDNERFLIMLNNEDAP 540 Query: 542 IDAHISALKPESTWINLELQQPADIGERGQLQ--LPAYGYVIL 582 ++ I A +P ++W + +P + E +Q LP YGY IL Sbjct: 541 VNVVIPAAQPGASWRTVN-GEPCAVVEESSIQAALPPYGYAIL 582 >B1D8P8.1 B1D8P8_9BACL Alpha amylase catalytic region Length = 583 Score = 703 bits (1815), Expect = 0.0, Method: Compositional matrix adjust. Identities = 338/583 (57%), Positives = 421/583 (72%), Gaps = 8/583 (1%) Query: 6 LESVYHRMGQNWSYAYDETVIHIRLRTKRDDVSRVDLICGDKYGWDRTHSEYAMERLSSD 65 LE++YHR QNW+YAY+ T +HIR+RTK+ D V+LI GDKY W T + M LSSD Sbjct: 3 LEAIYHRPKQNWAYAYNHTTLHIRIRTKKSDARSVELIAGDKYNWAGTFTITDMAVLSSD 62 Query: 66 NLFDYWQAAVRPPHRRLAYYFALHQDGNTVYFLEKGFLEHPPEIIYEGLFDFPILSPEDV 125 LFDYW+A++ PP RRL Y F L TV E GF P+ FDFP ++P DV Sbjct: 63 ALFDYWEASITPPFRRLRYAFRLTDGDETVIMTESGFPAELPDNP-NTFFDFPYINPVDV 121 Query: 126 HQPPAWVKDAVFYQIFPERFANGDPSNDPEGVLEWGGTPSPTNFFGGDLQGVLEHLDYLC 185 PPAWVKDAVFYQIFPERFANGDPS +PE WGG P+PTNFFGGDLQGVL+HLDYL Sbjct: 122 FSPPAWVKDAVFYQIFPERFANGDPSLNPETTEAWGGKPTPTNFFGGDLQGVLDHLDYLA 181 Query: 186 ALGINAIYFNPLFAATTNHKYDTADYLRIDPHFGTNELLKELVDACHAKGIRVLLDGVFN 245 LGINA+YF PLF ATTNHKYDT +Y+++D HFGTNE LKELV+ CH +GIR+LLD VFN Sbjct: 182 ELGINAVYFTPLFEATTNHKYDTENYMQVDRHFGTNEKLKELVEECHKRGIRLLLDAVFN 241 Query: 246 HCGHTFPPFMDVLENGRASRYADWFHVKEWPPVVNGGIPTYDTFAFEPIMPKFNTANPEV 305 H G TFPPF D+L+NG S YA+WFHV+EWP + GIPTY+TFAFEP+MPK NT NP+V Sbjct: 242 HAGRTFPPFADLLKNGEKSVYANWFHVREWPLRIEDGIPTYETFAFEPMMPKLNTENPDV 301 Query: 306 KEYLLKVGRYWIEEIGVDGWRLDVANEVSHQFWRDFRTMVKQINPDAYLVGEIMHDSLPW 365 K YL V RYWIE+IG+DGWRLDVANEV HQFWR+FR VK + PDAY++GEI HD++ W Sbjct: 302 KAYLFNVARYWIEKIGIDGWRLDVANEVDHQFWREFRQAVKSVKPDAYILGEIWHDAMMW 361 Query: 366 LLGDQFDAVMNYQLTNMLLNFFARGQTSAGQFSRSVGTLLANYPQQITEVSFNLLDSHDT 425 L GDQFDAVMNY TN +L+FF + F+ ++G LA YPQQ+TE +FNLLDSHDT Sbjct: 362 LEGDQFDAVMNYPFTNAVLDFFGHQTINGKTFADAIGRQLAAYPQQVTETAFNLLDSHDT 421 Query: 426 VRFLTLCGGDIRRLKLAFLFLMTYQGTPCIYYGDEIGMDGEYDPHNRKCMEWEPRKQNQD 485 R LT+CG D +KLA LF +TY GTPCIYYGDE+GMDG+ DP RKCM WEP +Q+++ Sbjct: 422 PRLLTICGDDADSMKLAALFQLTYPGTPCIYYGDEVGMDGDGDPDCRKCMVWEPEQQDRE 481 Query: 486 LLEYYRWAIRLRKANSAFRSSAIKFLEHP--EHPELLVYERWDEHDRFLVIMNNSELPID 543 LL +Y+ A+ LR ++A RS+ I+FL+ E ++VYER + +R LV MN Sbjct: 482 LLAFYKQALALRSKHAALRSADIRFLQAADGEDGGVIVYERREGDERILVAMNARG---R 538 Query: 544 AHISALKPESTWINL--ELQQPADIGERGQLQLPAYGYVILRE 584 ++A+ +W++L + Q+ + QL+LP YGY I ++ Sbjct: 539 EAVAAVPSGGSWVSLLGDEQRLEPAAGKLQLRLPGYGYGIWKQ 581 >Q84AP9.1 Q84AP9_9BACL Cyclodextrinase enzyme Length = 653 Score = 682 bits (1760), Expect = 0.0, Method: Compositional matrix adjust. Identities = 331/586 (56%), Positives = 414/586 (70%), Gaps = 9/586 (1%) Query: 4 IHLESVYHRMGQNWSYAYDETVIHIRLRTKRDDVSRVDLICGDKYGWDRTHSEYAMERLS 63 + LE+VYHR +NWSYAY+ T +H+R+RTK+DD++ V + GDKY WD T M +L+ Sbjct: 1 MFLEAVYHRPRKNWSYAYNGTTVHLRIRTKKDDMTAVYALAGDKYMWDHTMEYVPMTKLA 60 Query: 64 SDNLFDYWQAAVRPPHRRLAYYFALHQDGNTVYFLEKGFLEHPPEIIYEGLFDFPILSPE 123 +D LFDYW+ V PP+RR+ Y F L Q + E FL PP + LF++P ++P Sbjct: 61 TDELFDYWECEVTPPYRRVKYGFLLQQGHEKRWMTEYDFLTEPPANP-DRLFEYPFINPV 119 Query: 124 DVHQPPAWVKDAVFYQIFPERFANGDPSNDPEGVLEWGGT-PSPTNFFGGDLQGVLEHLD 182 DV QPPAWVKDA+FYQIFPERFANGD NDPEG L WG P+P+ FFGGDLQGV++HLD Sbjct: 120 DVFQPPAWVKDAIFYQIFPERFANGDTRNDPEGTLPWGSADPTPSCFFGGDLQGVIDHLD 179 Query: 183 YLCALGINAIYFNPLFAATTNHKYDTADYLRIDPHFGTNELLKELVDACHAKGIRVLLDG 242 +L LG+NA+YF PLF ATTNHKYDT DY +IDP FG + LK+LVD CH +GIRVLLD Sbjct: 180 HLSKLGVNAVYFTPLFKATTNHKYDTEDYFQIDPQFGDKDTLKKLVDLCHERGIRVLLDA 239 Query: 243 VFNHCGHTFPPFMDVLENGRASRYADWFHVKEWPPVVNGGIPTYDTFAFEPIMPKFNTAN 302 VFNH G TFPPF+DVL+NG S+Y DWFH++ P V GIPTYDTFAFEP+MPK NT + Sbjct: 240 VFNHSGRTFPPFVDVLKNGEKSKYKDWFHIRSLPLEVVDGIPTYDTFAFEPLMPKLNTEH 299 Query: 303 PEVKEYLLKVGRYWIEEIGVDGWRLDVANEVSHQFWRDFRTMVKQINPDAYLVGEIMHDS 362 PEVKEYLLK YWI E G+DGWRLDVANEVSHQFWR+FR +VKQ NPDAY++G++ H+S Sbjct: 300 PEVKEYLLKAAEYWIRETGIDGWRLDVANEVSHQFWREFRRVVKQANPDAYILGDVWHES 359 Query: 363 LPWLLGDQFDAVMNYQLTNMLLNFFARGQTSAGQFSRSVGTLLANYPQQITEVSFNLLDS 422 WL GDQFDAVMNY TN +L+FF A +FS +G LA YP+Q +EV FNLLDS Sbjct: 360 SIWLEGDQFDAVMNYPFTNAVLDFFIHQIADAEKFSFMLGKQLAGYPRQASEVMFNLLDS 419 Query: 423 HDTVRFLTLCGGDIRRLKLAFLFLMTYQGTPCIYYGDEIGMDGEYDPHNRKCMEWEPRKQ 482 HDT R LT GD R++KLA LF TY GTPCIYYGDE+G+DG +DP RKCMEW+ K Sbjct: 420 HDTARLLTQADGDKRKMKLAVLFQFTYFGTPCIYYGDEVGLDGGHDPGCRKCMEWDETKH 479 Query: 483 NQDLLEYYRWAIRLRKANSAFRSSAIKFLEHPEHPELLVYERWDEHDRFLVIMNNSELPI 542 ++DL +Y+ IRLR+A++A R+ KFL ++ + Y R D+ D LV+MNN + Sbjct: 480 DKDLFAFYQTVIRLRQAHAALRTGTFKFLTAEKNSRQIAYLREDDQDTILVVMNNDKA-- 537 Query: 543 DAHISALKPE-STWINLELQQPADIGERGQL--QLPAYGYVILRET 585 H L + W +L GQL +LPAYG+ +L+ + Sbjct: 538 -GHTLTLPVRHAQWTHL-WNDDVLTAAHGQLTVKLPAYGFAVLKAS 581 >B3K4I1.1 B3K4I1_9BACI Alpha amylase catalytic region Length = 582 Score = 676 bits (1745), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/583 (56%), Positives = 412/583 (70%), Gaps = 7/583 (1%) Query: 4 IHLESVYHRMGQNWSYAYDETVIHIRLRTKRDDVSRVDLICGDKYGWDRTHSEYAMERLS 63 + LE++YHR +NWSYAY+ T IH+R+RTK+DD++ V + GDKY WD+T M +LS Sbjct: 1 MFLEAIYHRPRKNWSYAYNGTTIHLRIRTKKDDLTEVYALAGDKYLWDQTMEYIPMTKLS 60 Query: 64 SDNLFDYWQAAVRPPHRRLAYYFALHQDGNTVYFLEKGFLEHPPEIIYEGLFDFPILSPE 123 SD +FDYW+ PP+RRL Y F L Q + E FL PP + LF++P ++P+ Sbjct: 61 SDEMFDYWECEATPPYRRLKYGFLLQQGDEKRWMTEYDFLTEPPANP-DRLFEYPFINPD 119 Query: 124 DVHQPPAWVKDAVFYQIFPERFANGDPSNDPEGVLEWGGT-PSPTNFFGGDLQGVLEHLD 182 DV PPAWVKDA+FYQIFPERFANGD SNDPEG L WG P+P NFFGGDLQGV++HLD Sbjct: 120 DVFHPPAWVKDAIFYQIFPERFANGDTSNDPEGALPWGSAEPTPKNFFGGDLQGVIDHLD 179 Query: 183 YLCALGINAIYFNPLFAATTNHKYDTADYLRIDPHFGTNELLKELVDACHAKGIRVLLDG 242 +L LG+NAIYF PLF ATTNHKYDT DY+ IDP FG + LK+LVD CHA+GIRVLLD Sbjct: 180 HLSELGVNAIYFTPLFTATTNHKYDTEDYMEIDPQFGDKDTLKKLVDLCHARGIRVLLDA 239 Query: 243 VFNHCGHTFPPFMDVLENGRASRYADWFHVKEWPPVVNGGIPTYDTFAFEPIMPKFNTAN 302 VFNH G TF PF+DV +NG S+Y DWF+V+ P V GIPTYDTFAFEP+MPK NT N Sbjct: 240 VFNHSGRTFAPFVDVQKNGLNSKYKDWFYVRSLPLEVVDGIPTYDTFAFEPLMPKLNTEN 299 Query: 303 PEVKEYLLKVGRYWIEEIGVDGWRLDVANEVSHQFWRDFRTMVKQINPDAYLVGEIMHDS 362 PEV+ YLLK YWI+E G+DGWRLDVANEV H FWR+FR +VK+ NPDAY++GE+ H+S Sbjct: 300 PEVRAYLLKAAEYWIKETGIDGWRLDVANEVGHSFWREFRQVVKKANPDAYILGEVWHES 359 Query: 363 LPWLLGDQFDAVMNYQLTNMLLNFFARGQTSAGQFSRSVGTLLANYPQQITEVSFNLLDS 422 WL GDQFDAVMNY TN +L+FF A +FS +G LA YP+Q +EV FNLLDS Sbjct: 360 SIWLEGDQFDAVMNYPFTNAVLDFFINRTADAEKFSFMLGKQLAGYPRQASEVMFNLLDS 419 Query: 423 HDTVRFLTLCGGDIRRLKLAFLFLMTYQGTPCIYYGDEIGMDGEYDPHNRKCMEWEPRKQ 482 HDT R LT GD R +KLA +F TY GTPCIYYGDEIGMDG +DP RKCMEW+ K Sbjct: 420 HDTARLLTQANGDKRLMKLAAMFQFTYFGTPCIYYGDEIGMDGGHDPGCRKCMEWDESKH 479 Query: 483 NQDLLEYYRWAIRLRKANSAFRSSAIKFLEHPEHPELLVYERWDEHDRFLVIMNNSELPI 542 ++DL +Y+ I+LRK +SA R+ KFL+ ++ + Y R D+++ L+ +NN + Sbjct: 480 DKDLFHFYQTLIKLRKEHSALRTGTFKFLKAEKNGGKIAYLREDDNETILIAVNNDKAGN 539 Query: 543 DAHISALKPESTWINLELQQPADIGERGQL--QLPAYGYVILR 583 + L + W NL ++GQL +LPAYG+ IL+ Sbjct: 540 TMTLPVL--DRQWTNL-WNGEGLSAKQGQLTVKLPAYGFAILK 579 >Q59226.1 Q59226_BACSP Cyclomaltodextrinase EC=3.2.1.54 Length = 558 Score = 675 bits (1742), Expect = 0.0, Method: Compositional matrix adjust. Identities = 318/537 (59%), Positives = 394/537 (73%), Gaps = 2/537 (0%) Query: 4 IHLESVYHRMGQNWSYAYDETVIHIRLRTKRDDVSRVDLICGDKYGWDRTHSEYAMERLS 63 + LE+VYHR +NWSYAY+ T +H+R+RTK+DD++ V + GDKY WD T M +L+ Sbjct: 1 MFLEAVYHRPRKNWSYAYNGTTVHLRIRTKKDDMTAVYALAGDKYMWDHTMEYVPMTKLA 60 Query: 64 SDNLFDYWQAAVRPPHRRLAYYFALHQDGNTVYFLEKGFLEHPPEIIYEGLFDFPILSPE 123 +D LFDYW+ V PP+RR+ Y F L Q + E FL PP + LF++P ++P Sbjct: 61 TDELFDYWECEVTPPYRRVKYGFLLQQGHEKRWMTEYDFLTEPPRNP-DRLFEYPFINPV 119 Query: 124 DVHQPPAWVKDAVFYQIFPERFANGDPSNDPEGVLEWGGT-PSPTNFFGGDLQGVLEHLD 182 DV QPPAWVKDA+FYQIFPERFANGD NDPEG L WG P+P+ FFGGDLQGV++HLD Sbjct: 120 DVFQPPAWVKDAIFYQIFPERFANGDTRNDPEGTLPWGSADPTPSCFFGGDLQGVIDHLD 179 Query: 183 YLCALGINAIYFNPLFAATTNHKYDTADYLRIDPHFGTNELLKELVDACHAKGIRVLLDG 242 +L LG+NA+YF PLF ATTNHKYDT DY +IDP FG + LK+LVD CH +GIRVLLD Sbjct: 180 HLSKLGVNAVYFTPLFKATTNHKYDTEDYFQIDPQFGDKDTLKKLVDLCHERGIRVLLDA 239 Query: 243 VFNHCGHTFPPFMDVLENGRASRYADWFHVKEWPPVVNGGIPTYDTFAFEPIMPKFNTAN 302 VFNH G TFPPF+DVL+NG S+Y DWFH++ P V GIPTYDTFAFEP+MPK NT + Sbjct: 240 VFNHSGRTFPPFVDVLKNGEKSKYKDWFHIRSLPLEVVDGIPTYDTFAFEPLMPKLNTEH 299 Query: 303 PEVKEYLLKVGRYWIEEIGVDGWRLDVANEVSHQFWRDFRTMVKQINPDAYLVGEIMHDS 362 P+VKEYLLK YWI E G+DGWRLDVANEVSHQFWR+FR +VKQ NPDAY++GE+ H+S Sbjct: 300 PDVKEYLLKAAEYWIRETGIDGWRLDVANEVSHQFWREFRRVVKQANPDAYILGEVWHES 359 Query: 363 LPWLLGDQFDAVMNYQLTNMLLNFFARGQTSAGQFSRSVGTLLANYPQQITEVSFNLLDS 422 WL GDQFDAVMNY TN +L+FF A +FS +G LA YP+Q +EV FNLLDS Sbjct: 360 SIWLEGDQFDAVMNYPFTNAVLDFFIHQIADAEKFSFMLGKQLAGYPRQASEVMFNLLDS 419 Query: 423 HDTVRFLTLCGGDIRRLKLAFLFLMTYQGTPCIYYGDEIGMDGEYDPHNRKCMEWEPRKQ 482 HDT R LT GD R++KLA LF TY GTPCIYYGDE+G+DG +DP RKCMEW+ K Sbjct: 420 HDTARLLTQADGDKRKMKLAVLFQFTYFGTPCIYYGDEVGLDGGHDPGCRKCMEWDETKH 479 Query: 483 NQDLLEYYRWAIRLRKANSAFRSSAIKFLEHPEHPELLVYERWDEHDRFLVIMNNSE 539 ++DL +Y+ IRLR+A++A R+ KFL ++ + Y R D+ D LV+MNN + Sbjct: 480 DKDLFAFYQTVIRLRQAHAALRTGTFKFLTAEKNSRQIAYLREDDQDTILVVMNNDK 536 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.141 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 4,003,515,099 Number of extensions: 184221927 Number of successful extensions: 370382 Number of sequences better than 1.0e-05: 4613 Number of HSP's gapped: 359237 Number of HSP's successfully gapped: 4842 Length of query: 585 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 444 Effective length of database: 1,732,333,088 Effective search space: 769155891072 Effective search space used: 769155891072 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)