BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0001.p (261 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KDI4.1 B3KDI4_9BACI Alpha/beta hydrolase fold 367 e-100 C0ZAH7.1 C0ZAH7_BREBN Putative uncharacterized protein 260 1e-67 A6CS44.1 A6CS44_9BACI Hydrolase, alpha/beta fold family protein 251 6e-65 B1DH28.1 B1DH28_9BACL Alpha/beta hydrolase fold 236 2e-60 B1D838.1 B1D838_9BACL Alpha/beta hydrolase fold 231 4e-59 >B3KDI4.1 B3KDI4_9BACI Alpha/beta hydrolase fold Length = 263 Score = 367 bits (943), Expect = e-100, Method: Compositional matrix adjust. Identities = 174/258 (67%), Positives = 210/258 (81%), Gaps = 4/258 (1%) Query: 1 MEIVRCEGSNICYSDQGTGDVIVLLHGFCGSSSYWEQVIPLLSGNYRVIAPDLRGHGVSD 60 ME V+C GS ICY DQG G+ ++LLHGFCGSS+YWEQV+P L G +RVI PDLRGHG SD Sbjct: 1 MEKVQCNGSTICYVDQGQGEPVILLHGFCGSSAYWEQVLPYLQG-FRVIVPDLRGHGRSD 59 Query: 61 APLGAYSIDQMADDVLSLLDALEIPECYLLGHSLGGYITLSFAQRHASRLKGFGLIHSTG 120 AP+G+Y+I+QMADDVL L+D L+IP+ LLGHSLGGYI LSFAQR+ASRL GFGLIHSTG Sbjct: 60 APMGSYTIEQMADDVLLLMDELDIPKAALLGHSLGGYIALSFAQRYASRLNGFGLIHSTG 119 Query: 121 YPDSGEARENRLKSVSMIQNEGIFAFVDGLVPGLFAPG---AAPQLLERTKEIGYRTPPQ 177 YPDS EA+E R+K+VS IQ EGI AFVDGLVPGLFAP P+ ++R KEIGY TPPQ Sbjct: 120 YPDSDEAKEKRVKAVSTIQGEGITAFVDGLVPGLFAPDTKETKPECIQRAKEIGYLTPPQ 179 Query: 178 GAVGAAMAMRERPDRRDVISATALPVLLVAGAEDSKVTPEQTFTSNKPNVTQATISGVGH 237 GA+GAA+AMRERPDRRDV+SA+ LP+LLVAG +D + E+TFT++ NVT+ I G GH Sbjct: 180 GAIGAALAMRERPDRRDVLSASTLPILLVAGEKDGLIPAERTFTTDHDNVTRIVIEGAGH 239 Query: 238 MSMYEAPERLAEIIKDYV 255 MSM E PE+L E+I ++ Sbjct: 240 MSMMETPEKLGEVISSFM 257 >C0ZAH7.1 C0ZAH7_BREBN Putative uncharacterized protein Length = 263 Score = 260 bits (664), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 127/258 (49%), Positives = 170/258 (65%), Gaps = 3/258 (1%) Query: 2 EIVRCEGSNICYSDQGTGDVIVLLHGFCGSSSYWEQVIPLLSGNYRVIAPDLRGHGVSDA 61 E+ G I Y ++GTGD +VL+HGFCGSS+YW +++PLLS +RVIA DLRGHG S A Sbjct: 4 EVQLSNGIKIAYVEEGTGDSLVLIHGFCGSSAYWHKLVPLLSKTHRVIAIDLRGHGDSSA 63 Query: 62 PLGAYSIDQMADDVLSLLDALEIPECYLLGHSLGGYITLSFAQRHASRLKGFGLIHSTGY 121 P YSI++ ADD+ L++ L + + +L GHSLGGY+TL+FA ++A +L FGL+HST Y Sbjct: 64 PDEPYSIERFADDLALLVEELGLAKIHLFGHSLGGYVTLAFANQYADKLASFGLVHSTPY 123 Query: 122 PDSGEARENRLKSVSMIQNEGIFAFVDGLVPGLFAPGAAPQLLERT---KEIGYRTPPQG 178 PD A+ NR K I+ G+ F+ LVP LFAP + E KEIG+ T P G Sbjct: 124 PDDDAAKANRDKGADNIRQNGMEPFIKALVPKLFAPSHIDTMREEVQLAKEIGFATSPVG 183 Query: 179 AVGAAMAMRERPDRRDVISATALPVLLVAGAEDSKVTPEQTFTSNKPNVTQATISGVGHM 238 AV +AMR+R DR V+ T LPVLLVAG ED + E+TFT +K NV Q + GHM Sbjct: 184 AVQTLIAMRDRVDRNHVLQETTLPVLLVAGTEDQIIPAEKTFTVDKNNVEQVLLPDAGHM 243 Query: 239 SMYEAPERLAEIIKDYVH 256 M E+PE++AE K +++ Sbjct: 244 GMVESPEKMAEAFKSFLN 261 >A6CS44.1 A6CS44_9BACI Hydrolase, alpha/beta fold family protein Length = 261 Score = 251 bits (640), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 122/258 (47%), Positives = 166/258 (64%), Gaps = 4/258 (1%) Query: 1 MEIVRCEGSNICYSDQGTGDVIVLLHGFCGSSSYWEQVIPLLSGNYRVIAPDLRGHGVSD 60 M+ R + + Y D G G ++LLHGFCGSS YWE+V+PLL +RVIA DLRGHG S Sbjct: 1 MDTQRVGINELAYKDHGEGQTVILLHGFCGSSEYWEKVMPLLD-EFRVIAVDLRGHGESG 59 Query: 61 APLGAYSIDQMADDVLSLLDALEIPECYLLGHSLGGYITLSFAQRHASRLKGFGLIHSTG 120 P G YSI+ MA+D+ +D ++ + Y+ GHSLGGY+TLSFA+R +LKGFGLIHST Sbjct: 60 IPEGGYSIEDMANDINYFMDQKQLKDVYMFGHSLGGYVTLSFAERFPDKLKGFGLIHSTP 119 Query: 121 YPDSGEARENRLKSVSMIQNEGIFAFVDGLVPGLFAPGAAPQL---LERTKEIGYRTPPQ 177 PD ++ R +S+ I +EG+ +F+DGLVP LF +L ++ K+IGY T P Sbjct: 120 LPDDENGKDKRTESIKKIDSEGMESFIDGLVPNLFNQERLEELEDVIDNAKQIGYNTSPA 179 Query: 178 GAVGAAMAMRERPDRRDVISATALPVLLVAGAEDSKVTPEQTFTSNKPNVTQATISGVGH 237 GA AMR R D R V+ + +PVLLVAGA+DS V E+TF + V + + GH Sbjct: 180 GAKETLKAMRSRGDLRSVVQDSRIPVLLVAGAKDSIVPQEKTFIVDGDQVKKVVLEDSGH 239 Query: 238 MSMYEAPERLAEIIKDYV 255 M +YE PE+L+ IK ++ Sbjct: 240 MGIYEEPEKLSLEIKKFI 257 >B1DH28.1 B1DH28_9BACL Alpha/beta hydrolase fold Length = 294 Score = 236 bits (601), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 122/257 (47%), Positives = 170/257 (66%), Gaps = 8/257 (3%) Query: 8 GSNICYSDQGTGD--VIVLLHGFCGSSSYWEQVIPLLSGNYRVIAPDLRGHGVSDAPLGA 65 G ++ Y D T V+VLLHG+CGSS+YWEQV+ L+ + R+IA DLRGHG S G Sbjct: 35 GISLAYYDSKTVQQPVVVLLHGYCGSSAYWEQVVEPLARHARIIALDLRGHGRSSGETGP 94 Query: 66 YSIDQM---ADDVLSLLDALEIPECYLLGHSLGGYITLSFAQRHASRLKGFGLIHSTGYP 122 + M ADD+ ++LD L+I + +LGHSLGGYITL+FA+R +L FGL+HST P Sbjct: 95 EETNAMELYADDLSAMLDQLKINKACVLGHSLGGYITLAFAERFEEKLSAFGLVHSTPLP 154 Query: 123 DSGEARENRLKSVSMIQNEGIFAFVDGLVPGLFAP---GAAPQLLERTKEIGYRTPPQGA 179 DS A+ENR +V+ I+ +G+ AFV+GLVP L++P A P +ER+ IG T GA Sbjct: 155 DSDAAKENRDNAVAAIKKDGVGAFVEGLVPKLYSPDNKSAMPDQVERSIAIGRGTSASGA 214 Query: 180 VGAAMAMRERPDRRDVISATALPVLLVAGAEDSKVTPEQTFTSNKPNVTQATISGVGHMS 239 +GAA M+ERPDR +V+ T+LPVLL+AG +D + E+TF + NVT+ ++ GHM Sbjct: 215 IGAAKGMKERPDRTEVLKQTSLPVLLLAGEQDQIIPVERTFVIDGANVTREKLAEAGHMG 274 Query: 240 MYEAPERLAEIIKDYVH 256 M E PE AE + +++ Sbjct: 275 MVERPEAFAEAVLRFIN 291 >B1D838.1 B1D838_9BACL Alpha/beta hydrolase fold Length = 284 Score = 231 bits (589), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 115/273 (42%), Positives = 161/273 (58%), Gaps = 15/273 (5%) Query: 4 VRCEGSNICYSD--QGTGDVIVLLHGFCGSSSYWEQVIPLLSGNYRVIAPDLRGHGVSDA 61 V G ++ Y + Q I+LLHGFCGSS YW V PLL+ +YRVI P LRGHG + Sbjct: 6 VNVNGIDMVYEECGQENEKAIILLHGFCGSSHYWHNVCPLLTDHYRVIMPQLRGHGETSV 65 Query: 62 PLGAYSIDQMADDVLSLLDALEIPECYLLGHSLGGYITLSFAQRHASRLKGFGLIHSTGY 121 P Y+++ MADD+ +L++ L I + + GHSLGGY+TL+ A ++ +L GFGLIHST Sbjct: 66 PDNVYTMEVMADDINALIEKLGIDKTVVFGHSLGGYVTLALADKYPDKLAGFGLIHSTAL 125 Query: 122 PDSGEARENRLKSVSMIQNEGIFAFVDGLVPGLFAPGA---APQLLERTKEIGYRTPPQG 178 PD+ E + R + +S IQ EGI +V L+P LF P +E+ KE G + +G Sbjct: 126 PDTEEVKRKRYQDISFIQKEGIEPYVHDLIPKLFKESKRQDMPDEVEKVKEAGLKMSAKG 185 Query: 179 AVGAAMAMRERPDRRDVISATALPVLLVAGAEDSKVTPEQTFTSNK----------PNVT 228 A+ M RPDR ++ P+LLVAGAED V+P+ TF+ N P++ Sbjct: 186 AIHTLEGMMMRPDRSAMLHEACYPILLVAGAEDEVVSPDDTFSVNDKETPKSTYEYPHIQ 245 Query: 229 QATISGVGHMSMYEAPERLAEIIKDYVHAAVRK 261 + T GV HMS+ EAPE+LA +I +Y+ K Sbjct: 246 ENTFEGVAHMSLVEAPEQLARVIANYLRTLSEK 278 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,635,045,955 Number of extensions: 70354160 Number of successful extensions: 204455 Number of sequences better than 1.0e-05: 5685 Number of HSP's gapped: 203292 Number of HSP's successfully gapped: 5686 Length of query: 261 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 128 Effective length of database: 1,807,701,208 Effective search space: 231385754624 Effective search space used: 231385754624 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)