BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0167.0018.p (659 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KDM8.1 B3KDM8_9BACI Heavy metal translocating P-type ATPase 823 0.0 A0R9B4.1 A0R9B4_BACAH Cation-transporting ATPase, P-type 588 e-166 C2NCH9.1 C2NCH9_BACCE Cadmium-transporting ATPase 588 e-166 B3ZQ74.1 B3ZQ74_BACCE Heavy metal-transporting ATPase 588 e-166 C3EWJ5.1 C3EWJ5_BACTU Cadmium-transporting ATPase 586 e-165 >B3KDM8.1 B3KDM8_9BACI Heavy metal translocating P-type ATPase Length = 674 Score = 823 bits (2127), Expect = 0.0, Method: Compositional matrix adjust. Identities = 423/650 (65%), Positives = 503/650 (77%), Gaps = 19/650 (2%) Query: 29 PRTPR-PRRFDPKAMLSNAEMQXXXXXXXXXXIAWAVSGWSEIFSILLYIVSYSVGGWIK 87 PR PR P ++D +++LSNAE+ +AW S WSE S++LY++SY+VGGW+K Sbjct: 22 PREPRQPGKWDIRSLLSNAEVVAALGSGLLMLVAWLTSQWSEPLSVVLYVISYAVGGWVK 81 Query: 88 AKEGVETLVKERDLDVNLLMIAAALGAASIGYWNEGAMLIFIFALSGALESYTMERSKKD 147 AKEGVETL+KERDLDVNLLMIAAA+GAA+IGYWNEGAMLIFIFALSGALESYTMERS+KD Sbjct: 82 AKEGVETLLKERDLDVNLLMIAAAMGAAAIGYWNEGAMLIFIFALSGALESYTMERSRKD 141 Query: 148 ISSLMALKPATAVRIEKGGMSEVAIDLLAVGDQLLVRPGEIIPADGTVYRGESAVNQASI 207 ISSLM L+P TA+RIEKG M V+I+ L +GD +LV+PGE+IPADG +YRG S+VNQASI Sbjct: 142 ISSLMELQPETALRIEKGEMVSVSINKLEIGDLILVKPGELIPADGKIYRGSSSVNQASI 201 Query: 208 TGESLPVEKSAGSDVFAGTINGEGPLYIEVTKSAENTLFAKIIKMVEEAETEVPESQRFI 267 TGES+PV+KS G +VF GT+NGEG LYIEVT+SAE+TLFAKI+++VEEAE EVPESQRFI Sbjct: 202 TGESVPVDKSVGDEVFTGTLNGEGVLYIEVTQSAESTLFAKIVRLVEEAENEVPESQRFI 261 Query: 268 KRLESIYXXXXXXXXXXXXXLPPFLLDWSWSAAFYKAMVFLVVASPCALVSSIMPAMLSA 327 KR ESIY L +L W W FYKAMVFLVVASPCALVSSIMPAMLSA Sbjct: 262 KRFESIYARVVVIATVALIVLATPVLGWDWDTTFYKAMVFLVVASPCALVSSIMPAMLSA 321 Query: 328 ISKSARKGILFKGGVHLENMAKTSVVAFDKTGTLTEGLPQVTDFIAGEGYDRTELLAVSA 387 IS ARKG+LFKGG H+ENMA+T+VVAFDKTGTLT G P VTDFIAGEG+DR ELL +SA Sbjct: 322 ISNRARKGVLFKGGAHIENMARTAVVAFDKTGTLTLGSPVVTDFIAGEGWDRHELLGISA 381 Query: 388 SIEKLSRHPLAEAIVRKAXXXXXXXXXXXXSTSVTGWGIEGLIEGRLWRIGKSNLLDERN 447 SIE++S+HPLA AIV+ A S+TGWG++ + G W+IGKS++LDE Sbjct: 382 SIERMSKHPLALAIVQSAEKEGAPLQQVENGQSITGWGMQAEVAGVTWKIGKSDILDEGT 441 Query: 448 --------EDGDE----------QWKTLRRQLEKEGKTVSLILDVEKIAGMIALQDTVRP 489 D DE W ++R LE EGKTVS+IL + I GMIALQD VRP Sbjct: 442 GDPRSGAISDQDERLGAVQESRQHWLSIRNTLESEGKTVSVILKGDSIVGMIALQDAVRP 501 Query: 490 QAVAAVRKLQELGVKVAMLTGDRAATAEVIAGQTGVDLVFAGLLPEDKVTHIKALRKQYX 549 QA AV++LQ+LG+KVAMLTGDR ATAEVIA Q+GVD+VF+ LLPEDKVTHIK LR +Y Sbjct: 502 QAAMAVKRLQQLGIKVAMLTGDRKATAEVIAKQSGVDMVFSDLLPEDKVTHIKDLRARYG 561 Query: 550 XXXXXXXXXXXAPALATATVGIGMGMKGSGAALEIADVVLMNDNIEEIASTITLARRSQR 609 APALATATVGIGMG+ GSG ALE+AD VLMNDNI+EIA T+ +AR+ QR Sbjct: 562 HVVMVGDGVNDAPALATATVGIGMGVSGSGTALEVADAVLMNDNIDEIAGTVKVARKVQR 621 Query: 610 IVKQNMVFAVTVIATLMLSNFLQGIALPFGVVGHEGSTILVILNGLRLLR 659 IVKQNM+FA+ VI TL+ +NF++G+ALP GV+GHEGSTILVILNGLRLLR Sbjct: 622 IVKQNMIFAIIVILTLIAANFIEGVALPLGVIGHEGSTILVILNGLRLLR 671 >A0R9B4.1 A0R9B4_BACAH Cation-transporting ATPase, P-type Length = 641 Score = 588 bits (1515), Expect = e-166, Method: Compositional matrix adjust. Identities = 293/588 (49%), Positives = 409/588 (69%), Gaps = 5/588 (0%) Query: 73 ILLYIVSYSVGGWIKAKEGVETLVKERDLDVNLLMIAAALGAASIGYWNEGAMLIFIFAL 132 I YI++Y VGG+ KAKEG+E ++E++L+V +LM+ AA+GAA IGYW EGA+LIFIFAL Sbjct: 55 ITCYILAYIVGGYAKAKEGIEDTIEEKELNVEMLMLFAAIGAAMIGYWAEGAVLIFIFAL 114 Query: 133 SGALESYTMERSKKDISSLMALKPATAVRIEKGGMSEVAIDLLAVGDQLLVRPGEIIPAD 192 SGA+ESYT+ +S+K+IS+L+ L+P A+RI G V + L + D +L++PGE +PAD Sbjct: 115 SGAMESYTLSKSQKEISALLDLQPEEALRISNGTEERVPVGRLQINDIILIKPGERVPAD 174 Query: 193 GTVYRGESAVNQASITGESLPVEKSAGSDVFAGTINGEGPLYIEVTKSAENTLFAKIIKM 252 GT+Y GE+ +++A+ITGE +P EK G +VFAGT+N G + +++TK ++ TLF KII++ Sbjct: 175 GTIYSGETNIDEAAITGEPIPNEKKHGDEVFAGTVNLRGAIEVKITKPSDQTLFQKIIRL 234 Query: 253 VEEAETEVPESQRFIKRLESIYXXXXXXXXXXXXXLPPFLLDWSWSAAFYKAMVFLVVAS 312 V+ A++E SQ FI++ E Y +P FLLDWSW+ FY+AM+ LVVAS Sbjct: 235 VQSAQSEKSPSQLFIEKFEGTYVKGVLLVVALMMFVPHFLLDWSWNETFYRAMILLVVAS 294 Query: 313 PCALVSSIMPAMLSAISKSARKGILFKGGVHLENMAKTSVVAFDKTGTLTEGLPQVTDFI 372 PCALV++I PA LSAIS AR GILFKGG+HLE +A +AFDKTGTLTEG P VTD Sbjct: 295 PCALVAAITPATLSAISNGARNGILFKGGIHLERLASVKAIAFDKTGTLTEGKPTVTDVY 354 Query: 373 AGEGYDRTELLAVSASIEKLSRHPLAEAIVRKAXXXXXXXXXXXXST-SVTGWGIEGLIE 431 E E+L+++A+IE S HPLAE+IV+ A + VTG+G++G++E Sbjct: 355 VRENMTEKEVLSITAAIESHSTHPLAESIVKYAQHAHDITLQKPENVEDVTGFGLKGILE 414 Query: 432 GRLWRIGKSNLLDERNEDGDEQWKTLRRQLEKEGKTVSLILDVEKIAGMIALQDTVRPQA 491 + ++IGK++ + E + + LEKEGKTV I D E I G+IAL+DT+R + Sbjct: 415 NKAYKIGKADFIGEETKTFH---NGISASLEKEGKTVVYISDEEGILGLIALKDTLRQET 471 Query: 492 VAAVRKLQELGVKVAMLTGDRAATAEVIAGQTGVDLVFAGLLPEDKVTHIKALRKQYXXX 551 +AA+R+LQ +GV+ M+TGD TA+ IA ++ + +A LPE KV IK L+K+Y Sbjct: 472 IAAIRELQSIGVEAIMITGDNEETAKAIASESNIKEYYASCLPETKVETIKKLKKKYGTV 531 Query: 552 XXXXXXXXXAPALATATVGIGMGMKGSGAALEIADVVLMNDNIEEIASTITLARRSQRIV 611 APALATA++G+ MG +G+ ALE ADVVLM + + ++ I L++R RIV Sbjct: 532 AMVGDGINDAPALATASIGVAMG-EGTDVALETADVVLMKNELSRLSQAIRLSKRMNRIV 590 Query: 612 KQNMVFAVTVIATLMLSNFLQGIALPFGVVGHEGSTILVILNGLRLLR 659 KQN++F++ VIA L+ SNFLQ +ALPFGV+GHEGSTILVILNGLRLL+ Sbjct: 591 KQNVLFSLAVIAMLICSNFLQFLALPFGVIGHEGSTILVILNGLRLLK 638 >C2NCH9.1 C2NCH9_BACCE Cadmium-transporting ATPase Length = 641 Score = 588 bits (1515), Expect = e-166, Method: Compositional matrix adjust. Identities = 293/588 (49%), Positives = 409/588 (69%), Gaps = 5/588 (0%) Query: 73 ILLYIVSYSVGGWIKAKEGVETLVKERDLDVNLLMIAAALGAASIGYWNEGAMLIFIFAL 132 I YI++Y VGG+ KAKEG+E ++E++L+V +LM+ AA+GAA IGYW EGA+LIFIFAL Sbjct: 55 ITCYILAYIVGGYAKAKEGIEDTIEEKELNVEMLMLFAAIGAAMIGYWAEGAVLIFIFAL 114 Query: 133 SGALESYTMERSKKDISSLMALKPATAVRIEKGGMSEVAIDLLAVGDQLLVRPGEIIPAD 192 SGA+ESYT+ +S+K+IS+L+ L+P A+RI G V + L + D +L++PGE +PAD Sbjct: 115 SGAMESYTLSKSQKEISALLDLQPEEALRISNGTEERVPVGRLQINDIILIKPGERVPAD 174 Query: 193 GTVYRGESAVNQASITGESLPVEKSAGSDVFAGTINGEGPLYIEVTKSAENTLFAKIIKM 252 GT+Y GE+ +++A+ITGE +P EK G +VFAGT+N G + +++TK ++ TLF KII++ Sbjct: 175 GTIYSGETNIDEAAITGEPIPNEKKHGDEVFAGTVNLRGAIEVKITKPSDQTLFQKIIRL 234 Query: 253 VEEAETEVPESQRFIKRLESIYXXXXXXXXXXXXXLPPFLLDWSWSAAFYKAMVFLVVAS 312 V+ A++E SQ FI++ E Y +P FLLDWSW+ FY+AM+ LVVAS Sbjct: 235 VQSAQSEKSPSQLFIEKFEGTYVKGVLLVVALMMFVPHFLLDWSWNETFYRAMILLVVAS 294 Query: 313 PCALVSSIMPAMLSAISKSARKGILFKGGVHLENMAKTSVVAFDKTGTLTEGLPQVTDFI 372 PCALV++I PA LSAIS AR GILFKGG+HLE +A +AFDKTGTLTEG P VTD Sbjct: 295 PCALVAAITPATLSAISNGARNGILFKGGIHLERLASVKAIAFDKTGTLTEGKPTVTDVY 354 Query: 373 AGEGYDRTELLAVSASIEKLSRHPLAEAIVRKAXXXXXXXXXXXXST-SVTGWGIEGLIE 431 E E+L+++A+IE S HPLAE+IV+ A + VTG+G++G++E Sbjct: 355 VRENMTEKEVLSITAAIESHSTHPLAESIVKYAQHAHDITLQKPENVEDVTGFGLKGILE 414 Query: 432 GRLWRIGKSNLLDERNEDGDEQWKTLRRQLEKEGKTVSLILDVEKIAGMIALQDTVRPQA 491 + ++IGK++ + E + + LEKEGKTV I D E I G+IAL+DT+R + Sbjct: 415 NKAYKIGKADFIGEETKTFH---NGISASLEKEGKTVVYISDEEGILGLIALKDTLRQET 471 Query: 492 VAAVRKLQELGVKVAMLTGDRAATAEVIAGQTGVDLVFAGLLPEDKVTHIKALRKQYXXX 551 +AA+R+LQ +GV+ M+TGD TA+ IA ++ + +A LPE KV IK L+K+Y Sbjct: 472 IAAIRELQSIGVEAIMITGDNEETAKAIASESNIKEYYASCLPETKVETIKKLKKKYGTV 531 Query: 552 XXXXXXXXXAPALATATVGIGMGMKGSGAALEIADVVLMNDNIEEIASTITLARRSQRIV 611 APALATA++G+ MG +G+ ALE ADVVLM + + ++ I L++R RIV Sbjct: 532 AMVGDGINDAPALATASIGVAMG-EGTDVALETADVVLMKNELSRLSQAIRLSKRMNRIV 590 Query: 612 KQNMVFAVTVIATLMLSNFLQGIALPFGVVGHEGSTILVILNGLRLLR 659 KQN++F++ VIA L+ SNFLQ +ALPFGV+GHEGSTILVILNGLRLL+ Sbjct: 591 KQNVLFSLAVIAMLICSNFLQFLALPFGVIGHEGSTILVILNGLRLLK 638 >B3ZQ74.1 B3ZQ74_BACCE Heavy metal-transporting ATPase Length = 641 Score = 588 bits (1515), Expect = e-166, Method: Compositional matrix adjust. Identities = 293/588 (49%), Positives = 409/588 (69%), Gaps = 5/588 (0%) Query: 73 ILLYIVSYSVGGWIKAKEGVETLVKERDLDVNLLMIAAALGAASIGYWNEGAMLIFIFAL 132 I YI++Y VGG+ KAKEG+E ++E++L+V +LM+ AA+GAA IGYW EGA+LIFIFAL Sbjct: 55 ITCYILAYIVGGYAKAKEGIEDTIEEKELNVEMLMLFAAIGAAMIGYWAEGAVLIFIFAL 114 Query: 133 SGALESYTMERSKKDISSLMALKPATAVRIEKGGMSEVAIDLLAVGDQLLVRPGEIIPAD 192 SGA+ESYT+ +S+K+IS+L+ L+P A+RI G V + L + D +L++PGE +PAD Sbjct: 115 SGAMESYTLSKSQKEISALLDLQPEEALRISNGTEERVPVGRLQINDIILIKPGERVPAD 174 Query: 193 GTVYRGESAVNQASITGESLPVEKSAGSDVFAGTINGEGPLYIEVTKSAENTLFAKIIKM 252 GT+Y GE+ +++A+ITGE +P EK G +VFAGT+N G + +++TK ++ TLF KII++ Sbjct: 175 GTIYSGETNIDEAAITGEPIPNEKKHGDEVFAGTVNLRGAIEVKITKPSDQTLFQKIIRL 234 Query: 253 VEEAETEVPESQRFIKRLESIYXXXXXXXXXXXXXLPPFLLDWSWSAAFYKAMVFLVVAS 312 V+ A++E SQ FI++ E Y +P FLLDWSW+ FY+AM+ LVVAS Sbjct: 235 VQSAQSEKSPSQLFIEKFEGTYVKGVLLVVALMMFVPHFLLDWSWNETFYRAMILLVVAS 294 Query: 313 PCALVSSIMPAMLSAISKSARKGILFKGGVHLENMAKTSVVAFDKTGTLTEGLPQVTDFI 372 PCALV++I PA LSAIS AR GILFKGG+HLE +A +AFDKTGTLTEG P VTD Sbjct: 295 PCALVAAITPATLSAISNGARNGILFKGGIHLERLASVKAIAFDKTGTLTEGKPTVTDVY 354 Query: 373 AGEGYDRTELLAVSASIEKLSRHPLAEAIVRKAXXXXXXXXXXXXST-SVTGWGIEGLIE 431 E E+L+++A+IE S HPLAE+IV+ A + VTG+G++G++E Sbjct: 355 VRENMTEKEVLSITAAIESHSTHPLAESIVKYAQHAHDITLQKPENVEDVTGFGLKGILE 414 Query: 432 GRLWRIGKSNLLDERNEDGDEQWKTLRRQLEKEGKTVSLILDVEKIAGMIALQDTVRPQA 491 + ++IGK++ + E + + LEKEGKTV I D E I G+IAL+DT+R + Sbjct: 415 NKAYKIGKADFIGEETKTFH---NGISASLEKEGKTVVYISDEEGILGLIALKDTLRQET 471 Query: 492 VAAVRKLQELGVKVAMLTGDRAATAEVIAGQTGVDLVFAGLLPEDKVTHIKALRKQYXXX 551 +AA+R+LQ +GV+ M+TGD TA+ IA ++ + +A LPE KV IK L+K+Y Sbjct: 472 IAAIRELQSIGVEAIMITGDNEETAKAIASESNIKEYYASCLPETKVETIKKLKKKYGTV 531 Query: 552 XXXXXXXXXAPALATATVGIGMGMKGSGAALEIADVVLMNDNIEEIASTITLARRSQRIV 611 APALATA++G+ MG +G+ ALE ADVVLM + + ++ I L++R RIV Sbjct: 532 AMVGDGINDAPALATASIGVAMG-EGTDVALETADVVLMKNELSRLSQAIRLSKRMNRIV 590 Query: 612 KQNMVFAVTVIATLMLSNFLQGIALPFGVVGHEGSTILVILNGLRLLR 659 KQN++F++ VIA L+ SNFLQ +ALPFGV+GHEGSTILVILNGLRLL+ Sbjct: 591 KQNVLFSLAVIAMLICSNFLQFLALPFGVIGHEGSTILVILNGLRLLK 638 >C3EWJ5.1 C3EWJ5_BACTU Cadmium-transporting ATPase Length = 641 Score = 586 bits (1511), Expect = e-165, Method: Compositional matrix adjust. Identities = 296/592 (50%), Positives = 410/592 (69%), Gaps = 13/592 (2%) Query: 73 ILLYIVSYSVGGWIKAKEGVETLVKERDLDVNLLMIAAALGAASIGYWNEGAMLIFIFAL 132 I YI++Y VGG+ KAKEG+E ++E++L+V +LM+ AA+GAA IGYW EGA+LIFIFAL Sbjct: 55 ITCYILAYIVGGYAKAKEGIEDTIEEKELNVEMLMLFAAIGAAMIGYWAEGAILIFIFAL 114 Query: 133 SGALESYTMERSKKDISSLMALKPATAVRIEKGGMSEVAIDLLAVGDQLLVRPGEIIPAD 192 SGA+ESYT+ +S+K+IS+L+ L+P A+RI G V + L + D +L++PGE +PAD Sbjct: 115 SGAMESYTLSKSQKEISALLDLQPEEALRISNGTEERVPVGRLQINDIILIKPGERVPAD 174 Query: 193 GTVYRGESAVNQASITGESLPVEKSAGSDVFAGTINGEGPLYIEVTKSAENTLFAKIIKM 252 GT+Y GE+ +++A+ITGE +P EK G +VFAGT+N G + +++TK ++ TLF KII++ Sbjct: 175 GTIYSGETNIDEAAITGEPIPNEKKHGDEVFAGTVNLRGAIEVKITKPSDQTLFQKIIRL 234 Query: 253 VEEAETEVPESQRFIKRLESIYXXXXXXXXXXXXXLPPFLLDWSWSAAFYKAMVFLVVAS 312 V+ A++E SQ FI++ E Y +P FLLDWSW+ FY+AM+ LVVAS Sbjct: 235 VQSAQSEKSPSQLFIEKFEGTYVKGVLLVVALMMFVPHFLLDWSWNETFYRAMILLVVAS 294 Query: 313 PCALVSSIMPAMLSAISKSARKGILFKGGVHLENMAKTSVVAFDKTGTLTEGLPQVTDFI 372 PCALV++I PA LSAIS AR GILFKGG+HLE +A +AFDKTGTLTEG P VTD Sbjct: 295 PCALVAAITPATLSAISNGARNGILFKGGIHLERLASVKAIAFDKTGTLTEGKPTVTDVY 354 Query: 373 AGEGYDRTELLAVSASIEKLSRHPLAEAIVRKAXXXXXXXXXXXXST-SVTGWGIEGLIE 431 E E+L+++A+IE S HPLAE+IV+ A + VTG+G++G+ E Sbjct: 355 VRENMTEKEVLSITAAIESHSTHPLAESIVKYAQHTYDITLKKPENVEDVTGFGLKGIFE 414 Query: 432 GRLWRIGKSNLLDERNEDGDEQWKTLRR----QLEKEGKTVSLILDVEKIAGMIALQDTV 487 + ++IGK++ + GDE KT LEKEGKTV I D E I G+IAL+DT+ Sbjct: 415 SKAYKIGKADFI------GDET-KTFHNGISASLEKEGKTVVYISDEEGILGLIALKDTL 467 Query: 488 RPQAVAAVRKLQELGVKVAMLTGDRAATAEVIAGQTGVDLVFAGLLPEDKVTHIKALRKQ 547 R + +AA+R+LQ +GV+ M+TGD TA+ IA ++ + +A LPE KV IK L+++ Sbjct: 468 RQETIAAIRELQSIGVEAIMITGDNEETAKAIASESNIKEYYASCLPETKVETIKKLKEK 527 Query: 548 YXXXXXXXXXXXXAPALATATVGIGMGMKGSGAALEIADVVLMNDNIEEIASTITLARRS 607 Y APALATA++G+ MG +G+ ALE ADVVLM + + ++ I L++R Sbjct: 528 YGTVAMVGDGINDAPALATASIGVAMG-EGTDVALETADVVLMKNELSRLSQAIRLSKRM 586 Query: 608 QRIVKQNMVFAVTVIATLMLSNFLQGIALPFGVVGHEGSTILVILNGLRLLR 659 RIVKQN++F++ VIA L+ SNFLQ +ALPFGV+GHEGSTILVILNGLRLL+ Sbjct: 587 NRIVKQNVIFSLAVIAMLICSNFLQFLALPFGVIGHEGSTILVILNGLRLLK 638 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,254,210,058 Number of extensions: 119641309 Number of successful extensions: 432614 Number of sequences better than 1.0e-05: 8168 Number of HSP's gapped: 410639 Number of HSP's successfully gapped: 10034 Length of query: 659 Length of database: 3,060,696,203 Length adjustment: 143 Effective length of query: 516 Effective length of database: 1,713,491,058 Effective search space: 884161385928 Effective search space used: 884161385928 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)