BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0005.p (250 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K4E9.1 B3K4E9_9BACI Nitroreductase 304 4e-81 B9Z0P9.1 B9Z0P9_9NEIS Nitroreductase 225 3e-57 Q2BRI4.1 Q2BRI4_9GAMM Hypothetical NADPH-flavin oxidoreductase 216 1e-54 A6F1V4.1 A6F1V4_9ALTE Nitroreductase 211 3e-53 C2MIR7.1 C2MIR7_BACCE CR(VI) reductase 209 2e-52 >B3K4E9.1 B3K4E9_9BACI Nitroreductase Length = 246 Score = 304 bits (779), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 144/244 (59%), Positives = 181/244 (74%), Gaps = 1/244 (0%) Query: 5 NETLELLNRHTSVRQFQDKPVSDEQLAAIIGAGQMASTSSNVQAYTVIAVTEPDLKAQLS 64 N+T+ LL H S+R++ D+PVS+EQL A++ AGQMASTSSNVQAY+VIAVT P+ K +L+ Sbjct: 2 NDTISLLMNHRSIRKYSDRPVSEEQLRAVVAAGQMASTSSNVQAYSVIAVTSPEKKQRLA 61 Query: 65 KLSGNQAYIEQCPVFLVWCADLYRLREISGPHLQGAASYVGSTENLIVXXXXXXXXXXXX 124 L+GNQAYI +CPVFLVWCADLYRL++ + H+ +Y STEN IV Sbjct: 62 ALAGNQAYIVECPVFLVWCADLYRLKKSAEAHVADQETYEDSTENFIVATVDAALAAQNA 121 Query: 125 XXXXESLGLGIVYIGGVRNNIAELSELLGLPELVYPVFGMCLGYPAGVNGIRPRLPLQAV 184 ESLGLGIVYIGGVRN+IAE SE L LP+LVYPVFGMCLGYP G+RPRLP++AV Sbjct: 122 AIAAESLGLGIVYIGGVRNSIAEFSEALELPDLVYPVFGMCLGYPDQEPGLRPRLPVKAV 181 Query: 185 LHHNGYQADASVEQVKVYDEVTRDYMRERTGGQKDTPWSEMMAGRLAQPSRLQMKEFLLQ 244 LH + Y A++E+VK YD ++ DY+ +RT G+ PWSE+MA RLA+P RL MK+FL Sbjct: 182 LHRDSYDP-AALEEVKSYDRISSDYLSKRTHGKNSAPWSELMAKRLAEPVRLHMKDFLKG 240 Query: 245 KGFM 248 KGFM Sbjct: 241 KGFM 244 >B9Z0P9.1 B9Z0P9_9NEIS Nitroreductase Length = 245 Score = 225 bits (573), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 113/246 (45%), Positives = 152/246 (61%), Gaps = 2/246 (0%) Query: 5 NETLELLNRHTSVRQFQDKPVSDEQLAAIIGAGQMASTSSNVQAYTVIAVTEPDLKAQLS 64 N ++LL H S+R+F ++PVSDE + I+ GQ A+TSSN+QA TVI V + + +++ Sbjct: 2 NPVIQLLKSHRSIRKFTNQPVSDELINEIVACGQAAATSSNIQATTVIRVRNVEKREKIA 61 Query: 65 KLSGNQAYIEQCPVFLVWCADLYRLREISGPHLQGAASYVGSTENLIVXXXXXXXXXXXX 124 LSG QAY+ FLV+CADL+R + +QG G TE+ I+ Sbjct: 62 ALSGGQAYVASAGAFLVYCADLHRPQLAC--EMQGGQFSAGMTEHFIIATVDAALAAQNS 119 Query: 125 XXXXESLGLGIVYIGGVRNNIAELSELLGLPELVYPVFGMCLGYPAGVNGIRPRLPLQAV 184 ESLGLGI YIGG+RN+ E++ELLGLP+ VYP+FG+CLGYPA I+PRLPL V Sbjct: 120 VTAAESLGLGICYIGGIRNHPQEIAELLGLPDHVYPIFGLCLGYPAQDPEIKPRLPLSVV 179 Query: 185 LHHNGYQADASVEQVKVYDEVTRDYMRERTGGQKDTPWSEMMAGRLAQPSRLQMKEFLLQ 244 L YQ +E +K YDE R Y R RTGG KD+ WS M G + + SR M++FL + Sbjct: 180 LKEECYQNQNDLEGIKAYDEQLRTYYRSRTGGTKDSCWSVEMKGLVGKESRPHMRDFLSR 239 Query: 245 KGFMQK 250 +GF K Sbjct: 240 RGFSMK 245 >Q2BRI4.1 Q2BRI4_9GAMM Hypothetical NADPH-flavin oxidoreductase Length = 245 Score = 216 bits (550), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 110/246 (44%), Positives = 150/246 (60%), Gaps = 2/246 (0%) Query: 5 NETLELLNRHTSVRQFQDKPVSDEQLAAIIGAGQMASTSSNVQAYTVIAVTEPDLKAQLS 64 N+ +ELLN H S+R+F ++PVS Q+ + + AGQ A+TSS +QA TVI V + + LS Sbjct: 2 NQVIELLNSHRSIRKFTEQPVSQSQVNSFVAAGQAAATSSFIQACTVIQVNDTGKRESLS 61 Query: 65 KLSGNQAYIEQCPVFLVWCADLYRLREISGPHLQGAASYVGSTENLIVXXXXXXXXXXXX 124 + +GNQAY+++ PVFLV+CAD+ R + H A G TE I Sbjct: 62 EWAGNQAYVKEAPVFLVFCADMNRHKISCDMH--EAEMLSGFTEQFITATVDCGLFAQNV 119 Query: 125 XXXXESLGLGIVYIGGVRNNIAELSELLGLPELVYPVFGMCLGYPAGVNGIRPRLPLQAV 184 ES GLG+VYIGG+RN I ++E L LPELVYPVFGMCLGYP ++PRLPL+ V Sbjct: 120 AIAAESEGLGLVYIGGLRNKIDLVAEALELPELVYPVFGMCLGYPDQNPEVKPRLPLEVV 179 Query: 185 LHHNGYQADASVEQVKVYDEVTRDYMRERTGGQKDTPWSEMMAGRLAQPSRLQMKEFLLQ 244 L + Y + + + YD R+Y RTGGQKD WSE ++G L + +R M FL Sbjct: 180 LKQDAYDDSSDKDLIAEYDHQVREYYATRTGGQKDMSWSEQISGMLQKEARPHMLSFLQS 239 Query: 245 KGFMQK 250 +GF++K Sbjct: 240 RGFLKK 245 >A6F1V4.1 A6F1V4_9ALTE Nitroreductase Length = 250 Score = 211 bits (538), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 109/246 (44%), Positives = 147/246 (59%), Gaps = 2/246 (0%) Query: 5 NETLELLNRHTSVRQFQDKPVSDEQLAAIIGAGQMASTSSNVQAYTVIAVTEPDLKAQLS 64 N T+ELL H S+R+FQ++ + E +I AGQ A+TSS+VQAY++I V +P + L+ Sbjct: 7 NPTIELLKSHRSIRKFQNRRIPRELFEELIRAGQSAATSSHVQAYSIIHVVDPKNREALA 66 Query: 65 KLSGNQAYIEQCPVFLVWCADLYRLREISGPHLQGAASYVGSTENLIVXXXXXXXXXXXX 124 +LSG Q Y+ +C FLV+CAD+ R + + GA G TE L+V Sbjct: 67 ELSGGQRYVAECSNFLVFCADMKRATDAA--ERAGADVVRGMTEQLLVASIDTALVAQNV 124 Query: 125 XXXXESLGLGIVYIGGVRNNIAELSELLGLPELVYPVFGMCLGYPAGVNGIRPRLPLQAV 184 ES GLG+ YIGG+RNN AE+SELL LPE VYPVFGMCLGYP ++PRLPL ++ Sbjct: 125 ATAAESAGLGLCYIGGIRNNPAEVSELLRLPEHVYPVFGMCLGYPDQAPEVKPRLPLASI 184 Query: 185 LHHNGYQADASVEQVKVYDEVTRDYMRERTGGQKDTPWSEMMAGRLAQPSRLQMKEFLLQ 244 L + YQ++ E V +D Y + RTGG KDT WS+ + R M++FL Sbjct: 185 LKEDYYQSERDPELVAEFDTTMNTYYQARTGGTKDTNWSQQLKPLFTSKLRPHMRDFLRG 244 Query: 245 KGFMQK 250 KGF K Sbjct: 245 KGFEMK 250 >C2MIR7.1 C2MIR7_BACCE CR(VI) reductase Length = 268 Score = 209 bits (532), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 103/246 (41%), Positives = 150/246 (60%), Gaps = 3/246 (1%) Query: 5 NETLELLNRHTSVRQFQDKPVSDEQLAAIIGAGQMASTSSNVQAYTVIAVTEPDLKAQLS 64 NE + + +H SVR+++++P+ + + ++ A Q A++S VQAY+VI +T+ DLKA+L+ Sbjct: 26 NEMIHKMEQHVSVRKYKEEPIPKDVVERMVQAAQHAASSHFVQAYSVIYITDQDLKAKLA 85 Query: 65 KLSGNQAYIEQCPVFLVWCADLYRLREISGPHLQGAASYVGSTENLIVXXXXXXXXXXXX 124 +LSGN+ +++ C F V CADL RL H G E+ IV Sbjct: 86 ELSGNR-HVKDCAAFFVCCADLKRLEMACEKH--GTEIKHEGVEDFIVATVDASLFAQNL 142 Query: 125 XXXXESLGLGIVYIGGVRNNIAELSELLGLPELVYPVFGMCLGYPAGVNGIRPRLPLQAV 184 ESLG GI YIGG+RNN E+SELL LP+ VYPVFGM +G P +G++PRLP+ A+ Sbjct: 143 ALAAESLGYGICYIGGIRNNPGEVSELLHLPDKVYPVFGMTVGVPDEDHGVKPRLPIAAI 202 Query: 185 LHHNGYQADASVEQVKVYDEVTRDYMRERTGGQKDTPWSEMMAGRLAQPSRLQMKEFLLQ 244 LH NGY E + YDE Y +ER+ QK+ W+E M+ +++ R+ MKEFL + Sbjct: 203 LHENGYDEKKYDELLNEYDETMSAYYKERSSNQKNVTWTESMSSFMSKEKRMHMKEFLSE 262 Query: 245 KGFMQK 250 KGF +K Sbjct: 263 KGFNKK 268 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,347,889,296 Number of extensions: 50235109 Number of successful extensions: 123429 Number of sequences better than 1.0e-05: 591 Number of HSP's gapped: 122539 Number of HSP's successfully gapped: 593 Length of query: 250 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 118 Effective length of database: 1,817,122,223 Effective search space: 214420422314 Effective search space used: 214420422314 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)