BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0002.p (383 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K376.1 B3K376_9BACI Histidine kinase dimerisation and phosphoa... 404 e-111 C2WEV4.1 C2WEV4_BACCE Two-component sensor kinase yocF 364 6e-99 C2XJQ6.1 C2XJQ6_BACCE Two-component sensor kinase yocF 363 2e-98 C2WVP3.1 C2WVP3_BACCE Two-component sensor kinase yocF 363 2e-98 C3ETK8.1 C3ETK8_BACTK Two-component sensor kinase yocF 361 6e-98 >B3K376.1 B3K376_9BACI Histidine kinase dimerisation and phosphoacceptor region Length = 408 Score = 404 bits (1038), Expect = e-111, Method: Compositional matrix adjust. Identities = 206/378 (54%), Positives = 264/378 (69%), Gaps = 4/378 (1%) Query: 6 FRLFPQRFGFFPYMWLIYLLFPVINLQEEHGIKLVFGAAMLALFAVTYRQLYWATGRA-F 64 F +FP + GFFPY+WLIY+ PVIN+ E G K+ G A++ LF VTYRQLY+A G F Sbjct: 12 FEVFPTQLGFFPYLWLIYMSLPVINMLREEGWKMGIGFALIGLFLVTYRQLYFAAGTPRF 71 Query: 65 TLWLAVQMLIIVILSAAYTPYDLFMGFFTANFIGWYTGEKHYKMAYAVFAAMVLVLLITA 124 +WL +QM II IL Y P ++MGFFTANFIGWYT + + ++ +F + + LI Sbjct: 72 FVWLGIQMSIIFILCMWYHPTYMYMGFFTANFIGWYTDRRKFTISLVLFGIIETIPLIKN 131 Query: 125 TWGMALEDLLFLYPFMIIMLVSPFGIRSLHRKRMLEKELDQANEVIKELVKREERMRIAR 184 M +DLLF+ PF +IML SPFGIRSL+R++ LE+EL +ANE I+ L+K EERMRIAR Sbjct: 132 A-SMLGKDLLFMIPFFLIMLFSPFGIRSLNRRQELERELAEANEQIRNLIKGEERMRIAR 190 Query: 185 DLHDTLGHTLSLITLKSQLVEKLAAKDAGRTQAEAREIQRISRAALRQVRELVSEMRAVS 244 DLHDTLGHTLSLITLKSQLVEKLAAKD + Q EAREIQ SRAALRQVRELVS+MR + Sbjct: 191 DLHDTLGHTLSLITLKSQLVEKLAAKDPLKAQLEAREIQNTSRAALRQVRELVSDMRTAT 250 Query: 245 VAEELAEVGEMLRTADIAMEVEGDAALEGVSDLTQNILSLCIKEAVTNIVKHSGADRCRI 304 + EEL E +L +ADI + GD LEG+SDL NILSLC++EAVTN+VKHS A +CRI Sbjct: 251 LPEELLEAQIILESADIQLVSTGDPKLEGISDLAHNILSLCLREAVTNVVKHSQASQCRI 310 Query: 305 ALAVTEGEVQISIEDNGVGLHPQEQEGHGRSKGNGMKGMAERLSLIDXXXXXXXXXXXXT 364 + + E ++ + DNG+GL + +G + NG+KGMAERLSLI T Sbjct: 311 DIESLDNEWRLLVADNGIGLKGND-DGASSTDSNGLKGMAERLSLIG-GTVEAVSDPQGT 368 Query: 365 VLAVAIPRVVKERKDGVT 382 L++ +P ++K RK+ T Sbjct: 369 KLSIRVPVIIKNRKEDTT 386 >C2WEV4.1 C2WEV4_BACCE Two-component sensor kinase yocF Length = 376 Score = 364 bits (935), Expect = 6e-99, Method: Compositional matrix adjust. Identities = 176/381 (46%), Positives = 254/381 (66%), Gaps = 8/381 (2%) Query: 3 RRQFRLFPQRFGFFPYMWLIYLLFPVINLQEEHGIKLVFGAAMLALFAVTYRQLYWATGR 62 ++QF FP+ GFFPYMW +YLLFP+ NL + G KL+ G ML +F +TYRQLY+ + Sbjct: 4 KKQFEFFPKHMGFFPYMWFVYLLFPIYNLTQVSGWKLIMGIGMLIIFVITYRQLYFVQ-K 62 Query: 63 AFTLWLAVQMLIIVILSAAYTPYDLFMGFFTANFIGWYTGEKHYKMAYAVFAAMVLVLLI 122 F LW +QM++I + + Y P+ +F GFFTA+ +G+ +K +++ + M++ + Sbjct: 63 TFILWACIQMVLIFLFALLYNPFMIFFGFFTASAMGFAPSKKVFRVLFVFLIVMLVGFIF 122 Query: 123 TATWGMALEDLLFLYPFMIIMLVSPFGIRSLHRKRMLEKELDQANEVIKELVKREERMRI 182 + L+ + P I+ML++PFG+R+ ++K+ML+ +L +ANE IK+LVKREER RI Sbjct: 123 VYFNQLTTPSLVNIVPMFILMLLTPFGMRNFNQKKMLKVQLSEANEQIKDLVKREERQRI 182 Query: 183 ARDLHDTLGHTLSLITLKSQLVEKLAAKDAGRTQAEAREIQRISRAALRQVRELVSEMRA 242 ARDLHDTLGHTLSLITLKSQLVEKL K+ R EA+EI + SR AL+Q+REL+S+MR Sbjct: 183 ARDLHDTLGHTLSLITLKSQLVEKLIVKNPERASVEAKEITQTSRTALKQLRELISDMRM 242 Query: 243 VSVAEELAEVGEMLRTADIAMEVEGDAALEGVSDLTQNILSLCIKEAVTNIVKHSGADRC 302 ++V EEL ++ +L A I++EV+ A +S L QNIL +C++EAVTN+VKHS A C Sbjct: 243 ITVEEELEQIKAILHAATISLEVDQQANFSSLSPLAQNILGMCLREAVTNVVKHSKATTC 302 Query: 303 RIALAVTEGEVQISIEDNGVGLHPQEQEGHGRSKGNGMKGMAERLSLIDXXXXXXXXXXX 362 ++L T+GE+ + ++DNGVGL Q + GNG+ GM ERL+LID Sbjct: 303 TVSLFETQGELILQVKDNGVGLQKQSND------GNGILGMKERLALID-GTLELDEANS 355 Query: 363 XTVLAVAIPRVVKERKDGVTA 383 T+L V +P V++ KD V A Sbjct: 356 GTLLTVKVPIVIRTGKDEVRA 376 >C2XJQ6.1 C2XJQ6_BACCE Two-component sensor kinase yocF Length = 376 Score = 363 bits (931), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 175/381 (45%), Positives = 260/381 (68%), Gaps = 8/381 (2%) Query: 3 RRQFRLFPQRFGFFPYMWLIYLLFPVINLQEEHGIKLVFGAAMLALFAVTYRQLYWATGR 62 +++ +FP+ GFFPYMWL+YLLFP+ +L + G KLV G+ ML LF +TYRQLY+ + Sbjct: 4 KKRIEIFPKHMGFFPYMWLVYLLFPIYHLAQASGWKLVLGSGMLILFIITYRQLYFVQ-K 62 Query: 63 AFTLWLAVQMLIIVILSAAYTPYDLFMGFFTANFIGWYTGEKHYKMAYAVFAAMVLVLLI 122 F LW +QM++I + + Y P+ +F GFFTA+ +G+ +K +++ + A M+ + Sbjct: 63 TFILWACIQMILIFLFALFYNPFMIFFGFFTASAMGFAPSKKVFRVLLCLLAVMLGAFVF 122 Query: 123 TATWGMALEDLLFLYPFMIIMLVSPFGIRSLHRKRMLEKELDQANEVIKELVKREERMRI 182 + L+ + P I+ML++PFG+R+ ++K+ML +LD+ANE IK+LVKREER RI Sbjct: 123 VNMNQLTPTSLVNIVPMFILMLLTPFGMRNFNQKKMLRNQLDEANEQIKDLVKREERQRI 182 Query: 183 ARDLHDTLGHTLSLITLKSQLVEKLAAKDAGRTQAEAREIQRISRAALRQVRELVSEMRA 242 ARDLHDTLGHTLSLITLKSQLVEKL K+ R AEA+EI + SR AL+Q+REL+S+MR Sbjct: 183 ARDLHDTLGHTLSLITLKSQLVEKLIVKNPERASAEAKEITQTSRTALKQLRELISDMRM 242 Query: 243 VSVAEELAEVGEMLRTADIAMEVEGDAALEGVSDLTQNILSLCIKEAVTNIVKHSGADRC 302 ++V EEL ++ +L+ A+I +EV +A +S + QNI+ +C++EAVTN+VKHS A RC Sbjct: 243 ITVEEELEQIKAILQAANIELEVRQEARSSSLSPIEQNIVGMCLREAVTNVVKHSKATRC 302 Query: 303 RIALAVTEGEVQISIEDNGVGLHPQEQEGHGRSKGNGMKGMAERLSLIDXXXXXXXXXXX 362 +++ ++GE+ + +EDNG+GL Q + GNG++GM ER++LID Sbjct: 303 AVSVLESQGELILKVEDNGIGLTDQNHD------GNGIRGMKERIALID-GFVELGTINP 355 Query: 363 XTVLAVAIPRVVKERKDGVTA 383 T+L V +P V++ KD V A Sbjct: 356 GTLLTVKVPVVIRTGKDEVRA 376 >C2WVP3.1 C2WVP3_BACCE Two-component sensor kinase yocF Length = 376 Score = 363 bits (931), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 175/381 (45%), Positives = 260/381 (68%), Gaps = 8/381 (2%) Query: 3 RRQFRLFPQRFGFFPYMWLIYLLFPVINLQEEHGIKLVFGAAMLALFAVTYRQLYWATGR 62 +++ +FP+ GFFPYMWL+YLLFP+ +L + G KLV G+ ML LF +TYRQLY+ + Sbjct: 4 KKRIEIFPKHMGFFPYMWLVYLLFPIYHLAQASGWKLVLGSGMLILFIITYRQLYFVQ-K 62 Query: 63 AFTLWLAVQMLIIVILSAAYTPYDLFMGFFTANFIGWYTGEKHYKMAYAVFAAMVLVLLI 122 F LW +QM++I + + Y P+ +F GFFTA+ +G+ +K +++ + A M+ + Sbjct: 63 TFILWACIQMILIFLFALFYNPFMIFFGFFTASAMGFAPSKKVFRVLLCLLAVMLGAFVF 122 Query: 123 TATWGMALEDLLFLYPFMIIMLVSPFGIRSLHRKRMLEKELDQANEVIKELVKREERMRI 182 + L+ + P I+ML++PFG+R+ ++K+ML +LD+ANE IK+LVKREER RI Sbjct: 123 VNMNQLTPTSLVNIVPMFILMLLTPFGMRNFNQKKMLRNQLDEANEQIKDLVKREERQRI 182 Query: 183 ARDLHDTLGHTLSLITLKSQLVEKLAAKDAGRTQAEAREIQRISRAALRQVRELVSEMRA 242 ARDLHDTLGHTLSLITLKSQLVEKL K+ R AEA+EI + SR AL+Q+REL+S+MR Sbjct: 183 ARDLHDTLGHTLSLITLKSQLVEKLIVKNPERASAEAKEITQTSRTALKQLRELISDMRM 242 Query: 243 VSVAEELAEVGEMLRTADIAMEVEGDAALEGVSDLTQNILSLCIKEAVTNIVKHSGADRC 302 ++V EEL ++ +L+ A+I +EV +A +S + QNI+ +C++EAVTN+VKHS A RC Sbjct: 243 ITVEEELEQIKAILQAANIELEVRQEARSSSLSPIEQNIVGMCLREAVTNVVKHSKATRC 302 Query: 303 RIALAVTEGEVQISIEDNGVGLHPQEQEGHGRSKGNGMKGMAERLSLIDXXXXXXXXXXX 362 +++ ++GE+ + +EDNG+GL Q + GNG++GM ER++LID Sbjct: 303 AVSVLESQGELILKVEDNGIGLTDQNHD------GNGIRGMKERIALID-GFVELGTINP 355 Query: 363 XTVLAVAIPRVVKERKDGVTA 383 T+L V +P V++ KD V A Sbjct: 356 GTLLTVKVPVVIRTGKDEVRA 376 >C3ETK8.1 C3ETK8_BACTK Two-component sensor kinase yocF Length = 376 Score = 361 bits (927), Expect = 6e-98, Method: Compositional matrix adjust. Identities = 175/381 (45%), Positives = 258/381 (67%), Gaps = 8/381 (2%) Query: 3 RRQFRLFPQRFGFFPYMWLIYLLFPVINLQEEHGIKLVFGAAMLALFAVTYRQLYWATGR 62 +++ +FP+ GFFPYMWL+YLLFP+ +L + G KLV G+ ML LF +TYRQLY+ + Sbjct: 4 KKRIEIFPKHMGFFPYMWLVYLLFPIYHLAQASGWKLVLGSGMLILFIITYRQLYFVQ-K 62 Query: 63 AFTLWLAVQMLIIVILSAAYTPYDLFMGFFTANFIGWYTGEKHYKMAYAVFAAMVLVLLI 122 F LW +QM++I + + Y P+ +F GFFTA+ +G+ +K +++ M+ L Sbjct: 63 TFILWACIQMILIFLFALFYNPFMIFFGFFTASAMGFAPNKKVFRVLLCSLVIMLGAFLF 122 Query: 123 TATWGMALEDLLFLYPFMIIMLVSPFGIRSLHRKRMLEKELDQANEVIKELVKREERMRI 182 + L+ + P I+ML++PFG+R+ ++K+ML +LD+ANE IK+LVKREER RI Sbjct: 123 VNMNQITTTSLVNIVPMFILMLLTPFGMRNFNQKKMLRNQLDEANEQIKDLVKREERQRI 182 Query: 183 ARDLHDTLGHTLSLITLKSQLVEKLAAKDAGRTQAEAREIQRISRAALRQVRELVSEMRA 242 ARDLHDTLGHTLSLITLKSQLVEKL K+ R AEA+EI + SR AL+Q+REL+S+MR Sbjct: 183 ARDLHDTLGHTLSLITLKSQLVEKLIVKNPERASAEAKEITQTSRTALKQLRELISDMRM 242 Query: 243 VSVAEELAEVGEMLRTADIAMEVEGDAALEGVSDLTQNILSLCIKEAVTNIVKHSGADRC 302 ++V EEL ++ +L+ A+I +EV +A +S + QNI+ +C++EAVTN+VKHS A RC Sbjct: 243 ITVEEELEQIKAILQAANIELEVRQEARSSSLSPIEQNIVGMCLREAVTNVVKHSKATRC 302 Query: 303 RIALAVTEGEVQISIEDNGVGLHPQEQEGHGRSKGNGMKGMAERLSLIDXXXXXXXXXXX 362 +++ ++GE+ + +EDNG+GL Q + GNG++GM ER++LID Sbjct: 303 AVSVLESQGELILKVEDNGIGLTDQNHD------GNGIRGMKERIALID-GFVELGTINP 355 Query: 363 XTVLAVAIPRVVKERKDGVTA 383 T+L V +P V++ KD V A Sbjct: 356 GTLLTVKVPVVIRTGKDEVRA 376 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.138 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,913,364,598 Number of extensions: 70608127 Number of successful extensions: 390828 Number of sequences better than 1.0e-05: 2916 Number of HSP's gapped: 389112 Number of HSP's successfully gapped: 2918 Length of query: 383 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 246 Effective length of database: 1,770,017,148 Effective search space: 435424218408 Effective search space used: 435424218408 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)