BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0164.0015.p (299 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7L6.1 B3K7L6_9BACI Protein-(Glutamine-N5) methyltransferase, ... 377 e-103 B1DEJ3.1 B1DEJ3_9BACL Protein-(Glutamine-N5) methyltransferase, ... 267 1e-69 C0Z827.1 C0Z827_BREBN Putative uncharacterized protein 254 7e-66 C5D9N3.1 C5D9N3_9BACI Modification methylase, HemK family 217 1e-54 Q2BG34.1 Q2BG34_9BACI Modification methylase, HemK family protein 202 4e-50 >B3K7L6.1 B3K7L6_9BACI Protein-(Glutamine-N5) methyltransferase, release factor-specific Length = 296 Score = 377 bits (969), Expect = e-103, Method: Compositional matrix adjust. Identities = 200/295 (67%), Positives = 223/295 (75%), Gaps = 10/295 (3%) Query: 5 QSIREAFAEASSFLDKSGCNEPQRSSQLLLEHVLGLSGAAYYMALADPFPPEARSAWEAG 64 +SIREAF EASSFLD+ EPQR++QLLLEHVLGLSG +YYMAL DPFP R A E Sbjct: 12 RSIREAFVEASSFLDRMRVMEPQRNAQLLLEHVLGLSGTSYYMALTDPFPEGRRLALEEA 71 Query: 65 VTRRAAXXXXPVQYIIGEQEFYGRVFEVTPDVLIPRPETELLVEAILKYGAELWPDGLVA 124 +TR+A P QYIIGEQEFYGR F+VTP VLIPRPETELLVEA+LKYG EL P Sbjct: 72 ITRKAEGV--PAQYIIGEQEFYGRPFDVTPAVLIPRPETELLVEAVLKYGQELTPQ---- 125 Query: 125 APGGAARPLTAVDIGAGSGAISVTLAAEAPAWRVCAGDISPGALAVAGRNAQRHGTAVDL 184 P G L VDIG GSGAI++TLA ++ W V A DISP AL VA RNA++ G V+ Sbjct: 126 -PDGG---LKVVDIGTGSGAIAITLALQSKGWDVFASDISPDALEVAARNAKKLGAQVEF 181 Query: 185 RLGDLLEPFAGMETDILVSNPPYIPGGDIAGLQREVRDHEPRTALDGGDDGLDPYRRMME 244 R G+LLEPFAGM DILVSNPPYIP DI LQ EVRD+EPRTALDGG DGL+PYR MM Sbjct: 182 RQGNLLEPFAGMGPDILVSNPPYIPAEDIEELQPEVRDYEPRTALDGGPDGLNPYRIMMA 241 Query: 245 QLPLLPAPPRLVGFELGQGQAGQVAKLLRAAGHWSEIVTIDDLAGIPRHVLGIAR 299 QLP L APPRL+ FELG GQAG VA+LLR AG+W EIVT+ DLAGI RHVLGI R Sbjct: 242 QLPFLSAPPRLIAFELGMGQAGDVAELLRKAGYWEEIVTVPDLAGIDRHVLGIFR 296 >B1DEJ3.1 B1DEJ3_9BACL Protein-(Glutamine-N5) methyltransferase, release factor-specific Length = 283 Score = 267 bits (682), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 153/287 (53%), Positives = 182/287 (63%), Gaps = 23/287 (8%) Query: 25 EPQRSSQLLLEHVLGLSGAAYYMALADPFPPEARSAWEAGVTRRAAXXXXPVQYIIGEQE 84 EP+ +++ LL HVLG+ + +PFP + W + R+AA PVQYIIGE+ Sbjct: 2 EPRSNAERLLLHVLGIDRSTMLRDFGEPFPAAHAAEWVELIRRKAAGE--PVQYIIGEEW 59 Query: 85 FYGRVFEVTPDVLIPRPETELLVEAILKYGAELWPDGLVAAPGGAARPLTAVDIGAGSGA 144 FYGR F VTP LIPRPETELLVEA+L+ +LWP P GA P T VD+G G+GA Sbjct: 60 FYGRPFTVTPATLIPRPETELLVEAVLEAADKLWP------PDGAEVP-TVVDVGTGTGA 112 Query: 145 ISVTLAAEAPAWRVCAGDISPGALAVAGRNAQRHGTAVDLRL--GDLLEPFAG------- 195 I VTLA++ P WRV A D+SP ALAVA NA RH A + GDLL PFA Sbjct: 113 IGVTLASQRPRWRVSASDLSPDALAVARTNAARHEAAGRMAFVQGDLLAPFAKRGAAGAA 172 Query: 196 -----METDILVSNPPYIPGGDIAGLQREVRDHEPRTALDGGDDGLDPYRRMMEQLPLLP 250 + D+LVSNPPYIP D+ GLQ EVRD+EPR ALDGG DGLDPYRRM+ QLP L Sbjct: 173 LDAEDIRIDVLVSNPPYIPADDLPGLQPEVRDYEPRLALDGGADGLDPYRRMVGQLPSLA 232 Query: 251 APPRLVGFELGQGQAGQVAKLLRAAGHWSEIVTIDDLAGIPRHVLGI 297 PR+V FELG GQA VA LLR G W +I I D GI RHV+ + Sbjct: 233 QLPRIVAFELGMGQARDVAALLRNVGEWDDIRIITDYGGIERHVIAV 279 >C0Z827.1 C0Z827_BREBN Putative uncharacterized protein Length = 296 Score = 254 bits (649), Expect = 7e-66, Method: Compositional matrix adjust. Identities = 143/299 (47%), Positives = 178/299 (59%), Gaps = 13/299 (4%) Query: 3 DVQSIREAFAEASSFLDKSGCNEPQRSSQLLLEHVLGLSGAAYYMALADPFPPEARSAWE 62 DV +IREA ASSFL + G +P ++L++ H L + MA+ D E + Sbjct: 9 DVTTIREALLRASSFLREKGTKDPLFEAELMIRHCLDWDRTRFLMAMPDSIDAETLVKLD 68 Query: 63 AGVTRRAAXXXXPVQYIIGEQEFYGRVFEVTPDVLIPRPETELLVEAILKYGAELWPDGL 122 RRA P+QY+ GEQEFYGR F V P VLIPRPETE+LVE ++ LWP+ Sbjct: 69 GLCVRRA--NNEPLQYMFGEQEFYGRPFTVRPGVLIPRPETEILVEQVMAAATMLWPE-- 124 Query: 123 VAAPGGAARPLTAVDIGAGSGAISVTLAAEAPAWRVCAGDISPGALAVAGRNAQRHGTAV 182 + L VDIG GSGAI +TLA E P WRV D+S A A+A NA R G V Sbjct: 125 -------SEELAVVDIGTGSGAICITLALEKPQWRVTTVDLSLEATAIARENASRLGADV 177 Query: 183 DLRLGDLLEPF--AGMETDILVSNPPYIPGGDIAGLQREVRDHEPRTALDGGDDGLDPYR 240 GDL++P AG + D+LVSNPPYIP D+ L EVR HEPR ALDGG+DGLD YR Sbjct: 178 RFLQGDLVQPLLEAGEKVDVLVSNPPYIPSRDVEELDDEVRVHEPRLALDGGEDGLDCYR 237 Query: 241 RMMEQLPLLPAPPRLVGFELGQGQAGQVAKLLRAAGHWSEIVTIDDLAGIPRHVLGIAR 299 R+ E LP L +V FE+G QA VA L+RA+G E+ + DLAGI R V+G+ R Sbjct: 238 RLCEALPNLLKDRAVVAFEVGIYQARDVAALMRASGVMDEVEIVPDLAGIERVVIGVRR 296 >C5D9N3.1 C5D9N3_9BACI Modification methylase, HemK family Length = 288 Score = 217 bits (553), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 129/291 (44%), Positives = 170/291 (58%), Gaps = 14/291 (4%) Query: 6 SIREAFAEASSFLDKSGCNEPQRSSQLLLEHVLGLSGAAYYMALADPFPPEARSAWEAGV 65 I E ASSFL + G E R+++LLL H L ++ A + L DP R ++EA V Sbjct: 4 KIYEVLTWASSFLKQHGKEE--RAAELLLCHHLQITRAQLFARLRDPIDENVRQSFEADV 61 Query: 66 TRRAAXXXXPVQYIIGEQEFYGRVFEVTPDVLIPRPETELLVEAILKYGAELWPDGLVAA 125 R+ PVQ++IG ++FYGR F V +VLIPRPETE LVE +L +L+P Sbjct: 62 -RKHVYEHVPVQHLIGLEQFYGRPFLVNRNVLIPRPETEELVEGVLTRITQLFPGN---- 116 Query: 126 PGGAARPLTAVDIGAGSGAISVTLAAEAPAWRVCAGDISPGALAVAGRNAQRHGTAVDLR 185 + + VD+G GSGAI++TLA E + RV A DI+P AL VA RNA+R G V Sbjct: 117 -----KTIDVVDVGTGSGAIAITLALENKSLRVAAIDIAPEALEVAKRNAERLGADVAFI 171 Query: 186 LGDLLEPF--AGMETDILVSNPPYIPGGDIAGLQREVRDHEPRTALDGGDDGLDPYRRMM 243 GDLL+P A + D++VSNPPYIP +IA L V+D+EP AL GG DGLD YRR Sbjct: 172 CGDLLQPLVEASRKVDVVVSNPPYIPENEIASLSPVVKDYEPLRALSGGKDGLDFYRRFA 231 Query: 244 EQLPLLPAPPRLVGFELGQGQAGQVAKLLRAAGHWSEIVTIDDLAGIPRHV 294 +LP + LV FE+G GQ VA +LR +E+ + D+ G R V Sbjct: 232 RELPFVLKERALVAFEVGAGQGEAVAAILRQTFPQAEVEVVFDINGKDRMV 282 >Q2BG34.1 Q2BG34_9BACI Modification methylase, HemK family protein Length = 292 Score = 202 bits (513), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 126/298 (42%), Positives = 171/298 (57%), Gaps = 16/298 (5%) Query: 1 MPDV-QSIREAFAEASSFLDKSGCNEPQRSSQLLLEHVLGLSGAAYYMALADPFPPEARS 59 MPD + EA ASSFL+ +G + + ++LL H L +S + + + + Sbjct: 1 MPDQPMKVYEALKWASSFLETAGRD--SNAGEILLGHFLNMSRSRLLAEMREELDTRMQG 58 Query: 60 AWEAGVTRRAAXXXXPVQYIIGEQEFYGRVFEVTPDVLIPRPETELLVEAILKYGAELWP 119 A++ V A PVQYIIG++EFYGR F V +VLIPRPETE LV LK +L+ Sbjct: 59 AFKEAVQLHA--DGRPVQYIIGKEEFYGRTFLVNENVLIPRPETEELVLEALKRKEKLF- 115 Query: 120 DGLVAAPGGAARPLTAVDIGAGSGAISVTLAAEAPAWRVCAGDISPGALAVAGRNAQRHG 179 G AA A DIG GSGAI+VTL E+P +V A D+ +L A NA ++G Sbjct: 116 -GREAASAAA-------DIGTGSGAIAVTLKLESPDLQVTATDVYGPSLDTAKENADKNG 167 Query: 180 TAVDLRLGDLLEPF--AGMETDILVSNPPYIPGGDIAGLQREVRDHEPRTALDGGDDGLD 237 ++ LGDLL+PF + DI++SNPPYIP D+ G+ V ++EP AL G DGLD Sbjct: 168 AEIEFILGDLLQPFIEESRKFDIIISNPPYIPLSDMEGMSEVVTENEPHRALFAGQDGLD 227 Query: 238 PYRRMMEQLPLLPAPPRLVGFELGQGQAGQVAKLLRAAGHWSEIVTIDDLAGIPRHVL 295 YRR ME+LPL+ LVGFE+G GQ+ V++LLR A + I + D+ G R V Sbjct: 228 LYRRFMEELPLVLKEKSLVGFEVGAGQSKAVSELLRTAFPDANIDIVYDINGKDRMVF 285 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.138 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,948,666,636 Number of extensions: 87783077 Number of successful extensions: 243435 Number of sequences better than 1.0e-05: 2144 Number of HSP's gapped: 238704 Number of HSP's successfully gapped: 2155 Length of query: 299 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 164 Effective length of database: 1,788,859,178 Effective search space: 293372905192 Effective search space used: 293372905192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)