BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0164.0014.p (351 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7L7.1 B3K7L7_9BACI Peptide chain release factor 1 633 e-180 Q9K6F4.1 RF1_BACHD Peptide chain release factor 1 472 e-131 C1P8Q9.1 C1P8Q9_BACCO Peptide chain release factor 1 471 e-131 B7IQY2.1 B7IQY2_BACC2 Peptide chain release factor 1 468 e-130 Q4MGI8.1 Q4MGI8_BACCE Peptide chain release factor 1 468 e-130 >B3K7L7.1 B3K7L7_9BACI Peptide chain release factor 1 Length = 355 Score = 633 bits (1633), Expect = e-180, Method: Compositional matrix adjust. Identities = 304/350 (86%), Positives = 324/350 (92%) Query: 1 LQSLADRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMM 60 LQSLADRYEKLSELLCDPDVA+DSKKLRDYSKEQSDLQPA+EAY EYKNV+EELEAAK M Sbjct: 5 LQSLADRYEKLSELLCDPDVANDSKKLRDYSKEQSDLQPAYEAYVEYKNVIEELEAAKTM 64 Query: 61 QGEKLDDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAA 120 QGEK+DDEM+EMVKMEIE+L+ R+ L E+IR VI+EIRGAAGGDEAA Sbjct: 65 QGEKMDDEMREMVKMEIEELTQREQELAERIRILLLPKDPNDDKNVIIEIRGAAGGDEAA 124 Query: 121 LFASDLYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQ 180 LFASDLYRMYTRYAD QGW+VELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQ Sbjct: 125 LFASDLYRMYTRYADTQGWKVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQ 184 Query: 181 RIPTTESGGRIHTSTSTVSVMPEAEEFEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRV 240 RIPTTESGGRIHTSTSTV+VMPEAEE +IEI DKDIRVDTFCSSGAGGQSVNTTKSAVRV Sbjct: 185 RIPTTESGGRIHTSTSTVAVMPEAEEVDIEIHDKDIRVDTFCSSGAGGQSVNTTKSAVRV 244 Query: 241 THVPTGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAGERKSKVGTGDRSER 300 TH+PTGIVATCQDGKSQNSNKEKALQVLRARI DM RQEEEAKYAGERKSKVGTGDRSER Sbjct: 245 THIPTGIVATCQDGKSQNSNKEKALQVLRARIFDMMRQEEEAKYAGERKSKVGTGDRSER 304 Query: 301 IRTYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISALSIAEQAELMEKG 350 IRTYNFPQSRVTDHRIGLT+H+LDQVMNG+I EI+SAL++AEQAE MEKG Sbjct: 305 IRTYNFPQSRVTDHRIGLTVHKLDQVMNGEIEEIVSALTLAEQAEAMEKG 354 >Q9K6F4.1 RF1_BACHD Peptide chain release factor 1 Length = 356 Score = 472 bits (1215), Expect = e-131, Method: Compositional matrix adjust. Identities = 223/351 (63%), Positives = 286/351 (81%) Query: 1 LQSLADRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMM 60 LQSL +RYE+L+ELL DP+V SDS KLR+YSKEQ+D++ + Y EYK VM++ + AK M Sbjct: 5 LQSLDERYERLNELLSDPEVISDSNKLREYSKEQADMEETVQVYREYKTVMDQYQDAKEM 64 Query: 61 QGEKLDDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAA 120 +KLDDEM MVK EI++L++R+ LE K++ VIVE+RGAAGGDEA Sbjct: 65 LEDKLDDEMYAMVKEEIDELATRKEDLEAKLKVLLLPKDPNDDKNVIVEVRGAAGGDEAQ 124 Query: 121 LFASDLYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQ 180 LFA+DLY+MY RYA+AQGW++E+++ ++ +LGG+KE+IF +NG+GAYSK+K+E+GAHRVQ Sbjct: 125 LFAADLYKMYARYAEAQGWKIEVIEASSTELGGYKEIIFSVNGKGAYSKLKYENGAHRVQ 184 Query: 181 RIPTTESGGRIHTSTSTVSVMPEAEEFEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRV 240 R+P TESGGRIHTST+TV+V+PEAEE E++I +KDIRVDTF SSG GGQSVNTT SAVR+ Sbjct: 185 RVPQTESGGRIHTSTATVAVLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMSAVRL 244 Query: 241 THVPTGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAGERKSKVGTGDRSER 300 TH+PTGIV +CQD KSQ NKEKA++VLRARI D +QE +A+Y RKS VGTGDRSER Sbjct: 245 THLPTGIVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQTRKSAVGTGDRSER 304 Query: 301 IRTYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISALSIAEQAELMEKGE 351 IRTYNFPQSRVTDHRIGLTL +L+Q++ G + EII +L + EQAELM++ E Sbjct: 305 IRTYNFPQSRVTDHRIGLTLQKLEQILQGKLDEIIESLIVHEQAELMKQQE 355 >C1P8Q9.1 C1P8Q9_BACCO Peptide chain release factor 1 Length = 356 Score = 471 bits (1213), Expect = e-131, Method: Compositional matrix adjust. Identities = 221/348 (63%), Positives = 281/348 (80%) Query: 1 LQSLADRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMM 60 LQ++ DRYEKL+ELL DP++ +D KKLR+YSKEQSDLQ + Y EYK V ++L+ AK + Sbjct: 5 LQAVEDRYEKLNELLSDPEIVNDPKKLREYSKEQSDLQETVQVYREYKEVQKQLQDAKSL 64 Query: 61 QGEKLDDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAA 120 EKLD EMK+MVK EI +L S+ EE+++ VIVEIRGAAGGDEAA Sbjct: 65 LSEKLDPEMKDMVKEEIHELESKIPEYEERLKILLLPKDPNDEKNVIVEIRGAAGGDEAA 124 Query: 121 LFASDLYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQ 180 LFA DLYRMY RYA++QGW++++++ N +GG+KE++FM+NG+GAYSK+KFE+GAHRVQ Sbjct: 125 LFAGDLYRMYMRYAESQGWKIDVIEANVTGIGGYKEIVFMVNGKGAYSKLKFENGAHRVQ 184 Query: 181 RIPTTESGGRIHTSTSTVSVMPEAEEFEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRV 240 R+P TESGGRIHTST+TV+V+PEAEE E++I +KDIRVDTF SSG GGQSVNTT SAVR+ Sbjct: 185 RVPETESGGRIHTSTATVAVLPEAEEVEVDINEKDIRVDTFASSGPGGQSVNTTMSAVRL 244 Query: 241 THVPTGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAGERKSKVGTGDRSER 300 TH+PTGIV +CQD KSQ NKEKA++VLRARI D +QE +A+Y RKS VGTGDRSER Sbjct: 245 THIPTGIVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDANRKSAVGTGDRSER 304 Query: 301 IRTYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISALSIAEQAELME 348 IRTYNFPQ+RVTDHRIGLT+ +LDQV++G + EII AL + +Q++ +E Sbjct: 305 IRTYNFPQNRVTDHRIGLTIQKLDQVLDGKLDEIIDALILEDQSKKLE 352 >B7IQY2.1 B7IQY2_BACC2 Peptide chain release factor 1 Length = 358 Score = 468 bits (1205), Expect = e-130, Method: Compositional matrix adjust. Identities = 219/351 (62%), Positives = 282/351 (80%) Query: 1 LQSLADRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMM 60 LQ++ +RYEKL+ELL DP+V SD+ KLR+YSKEQSD+Q E Y EYK+V E+L+ AK M Sbjct: 8 LQAVENRYEKLNELLSDPEVISDTNKLREYSKEQSDIQETVEVYREYKDVREQLKDAKAM 67 Query: 61 QGEKLDDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAA 120 EKLD EM++MVK E+ +L S++ L E+++ VIVE+RGAAGGDEAA Sbjct: 68 LEEKLDAEMRDMVKEEVSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGAAGGDEAA 127 Query: 121 LFASDLYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQ 180 LFA DLYRMY+RYA+ QGW+ E+++ + +LGG+KE+IFMING+GA++K+KFE+GAHRVQ Sbjct: 128 LFAGDLYRMYSRYAEVQGWKTEIIEASYTELGGYKEIIFMINGKGAFAKLKFENGAHRVQ 187 Query: 181 RIPTTESGGRIHTSTSTVSVMPEAEEFEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRV 240 R+P TESGGRIHTST+TV+V+PEAEE EI+I +KD+RVDTF SSG GGQSVNTT SAVR+ Sbjct: 188 RVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNTTMSAVRL 247 Query: 241 THVPTGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAGERKSKVGTGDRSER 300 TH+PTG+V +CQD KSQ NKEKA++VLRAR+ D RQE +A+Y RK VGTGDRSER Sbjct: 248 THLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVGTGDRSER 307 Query: 301 IRTYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISALSIAEQAELMEKGE 351 IRTYNFPQ+RVTDHRIGLT+ +LDQ++ G + + I+AL + +QA+ ME E Sbjct: 308 IRTYNFPQNRVTDHRIGLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE 358 >Q4MGI8.1 Q4MGI8_BACCE Peptide chain release factor 1 Length = 358 Score = 468 bits (1205), Expect = e-130, Method: Compositional matrix adjust. Identities = 219/351 (62%), Positives = 282/351 (80%) Query: 1 LQSLADRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMM 60 LQ++ +RYEKL+ELL DP+V SD+ KLR+YSKEQSD+Q E Y EYK+V E+L+ AK M Sbjct: 8 LQAVENRYEKLNELLSDPEVISDTNKLREYSKEQSDIQETVEVYREYKDVREQLKDAKAM 67 Query: 61 QGEKLDDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAA 120 +KLD EM+EMVK E+ +L S++ L E+++ VIVE+RGAAGGDEAA Sbjct: 68 LEDKLDAEMREMVKEEVSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGAAGGDEAA 127 Query: 121 LFASDLYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQ 180 LFA DLYRMY+RYA+ QGW+ E+++ + +LGG+KE+IFMING+GA++K+KFE+GAHRVQ Sbjct: 128 LFAGDLYRMYSRYAEVQGWKTEIIEASYTELGGYKEIIFMINGKGAFAKLKFENGAHRVQ 187 Query: 181 RIPTTESGGRIHTSTSTVSVMPEAEEFEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRV 240 R+P TESGGRIHTST+TV+V+PEAEE EI+I +KD+RVDTF SSG GGQSVNTT SAVR+ Sbjct: 188 RVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNTTMSAVRL 247 Query: 241 THVPTGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAGERKSKVGTGDRSER 300 TH+PTG+V +CQD KSQ NKEKA++VLRAR+ D RQE +A+Y RK VGTGDRSER Sbjct: 248 THLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVGTGDRSER 307 Query: 301 IRTYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISALSIAEQAELMEKGE 351 IRTYNFPQ+RVTDHRIGLT+ +LDQ++ G + + I+AL + +QA+ ME E Sbjct: 308 IRTYNFPQNRVTDHRIGLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE 358 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.129 0.348 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,843,816,513 Number of extensions: 72970887 Number of successful extensions: 200209 Number of sequences better than 1.0e-05: 3236 Number of HSP's gapped: 195444 Number of HSP's successfully gapped: 3237 Length of query: 351 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 215 Effective length of database: 1,779,438,163 Effective search space: 382579205045 Effective search space used: 382579205045 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)