BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0164.0008.p (498 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7M1.1 B3K7M1_9BACI Phospholipase D/Transphosphatidylase 681 0.0 Q9K8Z4.1 CLS_BACHD Cardiolipin synthetase EC=2.7.8.- 536 e-150 O66043.1 CLS_BACPF Cardiolipin synthetase EC=2.7.8.- 530 e-148 C5D794.1 C5D794_9BACI Phospholipase D/Transphosphatidylase 520 e-145 C3FH06.1 C3FH06_BACTB Cardiolipin synthetase 2 515 e-144 >B3K7M1.1 B3K7M1_9BACI Phospholipase D/Transphosphatidylase Length = 502 Score = 681 bits (1758), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/477 (69%), Positives = 390/477 (81%), Gaps = 6/477 (1%) Query: 24 TVGTIVSVFSTLTVISIGLGIFMENRNPSTTMSWILLLALIPVVGLILYFLFGQNVFKRR 83 T GT++ +FSTLTV+SIG IFMENR+PS+TM+WILLLAL+P+VGL+ YFLFGQN FKRR Sbjct: 30 TTGTVIGIFSTLTVVSIGFMIFMENRHPSSTMAWILLLALVPIVGLVFYFLFGQNYFKRR 89 Query: 84 KYDKKAQRDQMAYERIENDALRMH-QDWSIFDTTRQKLLGLSQRLARTPISFNSETRIXX 142 KYDKKA++D + Y ++ LR + D S F Q+LL LS RLARTPISF+S+T++ Sbjct: 90 KYDKKARKDGLVYT---SEVLRKNTPDVSCFKPEVQQLLQLSTRLARTPISFSSDTKVLT 146 Query: 143 XXXXXXXXXXXXXRQAQHHIHMEYYIFRADHIGTRIQQILIEKARAGVAVRFMYDAVGSI 202 QA HHIHMEYYI+R D IGT+IQ+ILI KAR G+AVRFM DAVGS+ Sbjct: 147 DGEETFAALIAELEQAAHHIHMEYYIYRPDAIGTQIQEILIRKARQGIAVRFMVDAVGSL 206 Query: 203 QLSRAFLKELSDAGVQVAAYGN-STSFFSSRVNYRNHRKIVVIDGDVGFMGGLNVGDEYL 261 QL +FL+E+ DAGVQVA +G+ T FF+SRVNYRNHRKIVVIDG VGF+GGLNVGDEYL Sbjct: 207 QLPPSFLQEMRDAGVQVAVFGSPKTIFFTSRVNYRNHRKIVVIDGSVGFVGGLNVGDEYL 266 Query: 262 SRSKTYGFWRDTHMLLRGEAVRTMQIIFLQDWMHTTGEKILEQDYLSPQLRFTNGDGAVQ 321 SRSK +GFWRDTHML+RGEAVRT+Q++FLQDW + TGE+ E Y SP L N GAVQ Sbjct: 267 SRSKAFGFWRDTHMLVRGEAVRTLQVVFLQDWQYMTGEQPNEPIYYSPDL-LENRSGAVQ 325 Query: 322 IIASGPDNERRALKNIFFSMITSAEKSVWIATPYFIPDEDILTALRVAAMSGLDVRLLFP 381 IIASGPDNERRALKNIFFSMI SA KSVW+ATPYFIPDEDILTALRVAA+SGLDVR+LFP Sbjct: 326 IIASGPDNERRALKNIFFSMIVSARKSVWLATPYFIPDEDILTALRVAALSGLDVRILFP 385 Query: 382 AKPDKWLPFLASHSYFPALLESGVKIYEYEKGFIHSKLLITDGEIATIGTANMDMRSFHL 441 +KPDKWLPFLASHSYFPALL+ GV++YEYEKGF+HSKLLI DGE+ATIGTANMDMRSFHL Sbjct: 386 SKPDKWLPFLASHSYFPALLDVGVRVYEYEKGFMHSKLLIVDGEVATIGTANMDMRSFHL 445 Query: 442 NFEVNALLLQTESVSRIVADFERDLLSTRQIVHETFMNKRLLERVLESAARLMSPLL 498 NFEVNALL+ T+SVS++V+DFE DL S KR++ R+LESAARLMSPLL Sbjct: 446 NFEVNALLVHTDSVSKLVSDFEHDLESAIVYDRHHTRKKRIMTRLLESAARLMSPLL 502 >Q9K8Z4.1 CLS_BACHD Cardiolipin synthetase EC=2.7.8.- Length = 503 Score = 536 bits (1381), Expect = e-150, Method: Compositional matrix adjust. Identities = 257/477 (53%), Positives = 342/477 (71%), Gaps = 4/477 (0%) Query: 26 GTIVSVFS---TLTVISIGLGIFMENRNPSTTMSWILLLALIPVVGLILYFLFGQNVFKR 82 G IV FS T+TV+ IG+ IF+ENR+P+ T++W+++LA+ PVVG I Y +FGQN K Sbjct: 27 GWIVGAFSVLITITVVFIGIVIFLENRHPTKTLTWLMVLAVFPVVGFIFYLMFGQNHRKS 86 Query: 83 RKYDKKAQRDQMAYERIENDALRMHQDWSIFDTTRQKLLGLSQRLARTPISFNSETRIXX 142 + + KKA D+ A+E+IE + + +Q L L+ RLA PISF++ T++ Sbjct: 87 KTFMKKALSDEEAFEKIEGNRQLNEEQLQKMGGHQQLLFRLAHRLANNPISFSTNTKVLT 146 Query: 143 XXXXXXXXXXXXXRQAQHHIHMEYYIFRADHIGTRIQQILIEKARAGVAVRFMYDAVGSI 202 R A HHIH+EYYI R D IG I++IL++KA+ G+ VRF+YD VGS Sbjct: 147 DGKETFAHIKQALRMATHHIHLEYYIVRDDEIGQEIKEILMQKAKEGIHVRFLYDGVGSW 206 Query: 203 QLSRAFLKELSDAGVQVAAYGN-STSFFSSRVNYRNHRKIVVIDGDVGFMGGLNVGDEYL 261 +LS++++++L AGV++ + F + +NYRNHRKI+VIDG VGF+GGLN+GDEYL Sbjct: 207 KLSKSYIQDLKQAGVEIVPFAPVKLPFINHTINYRNHRKIIVIDGTVGFVGGLNIGDEYL 266 Query: 262 SRSKTYGFWRDTHMLLRGEAVRTMQIIFLQDWMHTTGEKILEQDYLSPQLRFTNGDGAVQ 321 + +GFWRDTH+ +RGEAVRT+Q+IFL+DW H TGE IL+ YLSP L DG VQ Sbjct: 267 GKDPYFGFWRDTHLYVRGEAVRTLQLIFLRDWAHETGETILKPSYLSPALTNMKDDGGVQ 326 Query: 322 IIASGPDNERRALKNIFFSMITSAEKSVWIATPYFIPDEDILTALRVAAMSGLDVRLLFP 381 +IASGPD K +FFSMITSA+KS+WI +PYFIPDEDIL+AL++AA+SG+DVR+L P Sbjct: 327 MIASGPDTRWEINKKLFFSMITSAKKSIWITSPYFIPDEDILSALKIAALSGIDVRILVP 386 Query: 382 AKPDKWLPFLASHSYFPALLESGVKIYEYEKGFIHSKLLITDGEIATIGTANMDMRSFHL 441 +PDK + F AS SYFP LLE+GVK+YEY +GF+HSK++I D EIA+IGT+NMDMRSFHL Sbjct: 387 NRPDKRIVFHASRSYFPELLEAGVKVYEYTRGFLHSKIIIVDNEIASIGTSNMDMRSFHL 446 Query: 442 NFEVNALLLQTESVSRIVADFERDLLSTRQIVHETFMNKRLLERVLESAARLMSPLL 498 NFEVNA L +T+SV+ +V+DF DL T QI E F N+ RVLES RL+SPLL Sbjct: 447 NFEVNAFLYRTKSVTTLVSDFVYDLEHTNQIRFEQFRNRAWYYRVLESTCRLLSPLL 503 >O66043.1 CLS_BACPF Cardiolipin synthetase EC=2.7.8.- Length = 503 Score = 530 bits (1364), Expect = e-148, Method: Compositional matrix adjust. Identities = 256/475 (53%), Positives = 346/475 (72%), Gaps = 2/475 (0%) Query: 25 VGTIVSVFSTLTVISIGLGIFMENRNPSTTMSWILLLALIPVVGLILYFLFGQNVFKRRK 84 VGT+ VF TL+VI IG+ IF ENR+P+ T++W+L+LA PVVG Y +FGQN K ++ Sbjct: 30 VGTLSVVF-TLSVIFIGIIIFFENRHPTKTLTWLLVLAAFPVVGFFFYLMFGQNHRKSKR 88 Query: 85 YDKKAQRDQMAYERIENDALRMHQDWSIFDTTRQKLLGLSQRLARTPISFNSETRIXXXX 144 + KKA D+ A+++IE + +Q L L+ +L + PISF+SET++ Sbjct: 89 FSKKAIEDERAFQKIEGQRQLNEEQLKKMGGHQQLLFRLAHKLGKNPISFSSETKVLTDG 148 Query: 145 XXXXXXXXXXXRQAQHHIHMEYYIFRADHIGTRIQQILIEKARAGVAVRFMYDAVGSIQL 204 + A+HHIH+EYYI R D +G +I+ ILI KA+ GV VRF+YD VGS +L Sbjct: 149 KETYAHILQALKMAEHHIHLEYYIVRHDDLGNQIKDILISKAKEGVHVRFLYDGVGSWKL 208 Query: 205 SRAFLKELSDAGVQVAAYGN-STSFFSSRVNYRNHRKIVVIDGDVGFMGGLNVGDEYLSR 263 S+++++EL DAGV++ ++ F + +NYRNHRKI+VIDG VGF+GGLN+GDEYL + Sbjct: 209 SKSYVEELRDAGVEMVSFSPVKLPFLTHTINYRNHRKIIVIDGVVGFVGGLNIGDEYLGK 268 Query: 264 SKTYGFWRDTHMLLRGEAVRTMQIIFLQDWMHTTGEKILEQDYLSPQLRFTNGDGAVQII 323 +G+WRDTH+ +RGEAVRT+Q+IFLQDW + TGE IL Q YLSP L T GDG VQ+I Sbjct: 269 DAYFGYWRDTHLYVRGEAVRTLQLIFLQDWHYQTGETILNQTYLSPSLSMTKGDGGVQMI 328 Query: 324 ASGPDNERRALKNIFFSMITSAEKSVWIATPYFIPDEDILTALRVAAMSGLDVRLLFPAK 383 ASGPD K +FFSMITSA+KS+WIA+PYFIPD+DIL+AL++AA+SG+DVR+L P + Sbjct: 329 ASGPDTRWEVNKKLFFSMITSAKKSIWIASPYFIPDDDILSALKIAALSGIDVRILVPNR 388 Query: 384 PDKWLPFLASHSYFPALLESGVKIYEYEKGFIHSKLLITDGEIATIGTANMDMRSFHLNF 443 PDK + F AS SYFP LLE+GVK+YEY +GF+HSK++I D EIA+IGT+NMDMRSFHLNF Sbjct: 389 PDKRIVFHASRSYFPELLEAGVKVYEYNRGFMHSKIIIVDHEIASIGTSNMDMRSFHLNF 448 Query: 444 EVNALLLQTESVSRIVADFERDLLSTRQIVHETFMNKRLLERVLESAARLMSPLL 498 EVNA L +T SV+++V+D+ DL + QI F N+ R++ES +RL+SPLL Sbjct: 449 EVNAYLYRTSSVTKLVSDYVYDLEHSNQINFSLFKNRPFFHRLIESTSRLLSPLL 503 >C5D794.1 C5D794_9BACI Phospholipase D/Transphosphatidylase Length = 502 Score = 520 bits (1339), Expect = e-145, Method: Compositional matrix adjust. Identities = 241/475 (50%), Positives = 342/475 (72%), Gaps = 10/475 (2%) Query: 29 VSVFSTLTVISIGLGIFMENRNPSTTMSWILLLALIPVVGLILYFLFGQNVFKRRKYDKK 88 +S+ + +V+ I I +ENR P+ T++W+++L P++G Y +FG+N K+R + KK Sbjct: 33 LSILISCSVVFIAFVISLENRKPAHTITWLVVLGSFPLIGFFFYLMFGRNYRKQRLFQKK 92 Query: 89 AQRDQMAYERIENDALRMHQDWSI----FDTTRQKLLGLSQRLARTPISFNSETRIXXXX 144 A D+ + + + ++W+I ++ LL L+ R+ ++P+S +ETR+ Sbjct: 93 AMLDEQTFLKFQG-----QREWAIEQMPIGEHQRPLLQLAHRIGKSPVSLATETRVLTNG 147 Query: 145 XXXXXXXXXXXRQAQHHIHMEYYIFRADHIGTRIQQILIEKARAGVAVRFMYDAVGSIQL 204 +A HHIH+EYYI R D +G +++ ILIEKA+ GV VRF+YDAVGS +L Sbjct: 148 EETFSTIFEELEKATHHIHLEYYIVRHDEVGQKLKTILIEKAKKGVHVRFLYDAVGSWKL 207 Query: 205 SRAFLKELSDAGVQVAAYGN-STSFFSSRVNYRNHRKIVVIDGDVGFMGGLNVGDEYLSR 263 S+ +++EL DAGV++ + F S+ +N+RNHRKI+VIDG +GF+GGLN+GDEYL + Sbjct: 208 SKTYIQELRDAGVEMIPFSPVRLPFLSNTINFRNHRKIIVIDGTIGFVGGLNIGDEYLGK 267 Query: 264 SKTYGFWRDTHMLLRGEAVRTMQIIFLQDWMHTTGEKILEQDYLSPQLRFTNGDGAVQII 323 K +GFWRDTH+ +RGEAVRT+Q+IFLQDW + TG+ +L +YLSP+L +G G VQ+I Sbjct: 268 DKYFGFWRDTHLWIRGEAVRTLQLIFLQDWYYMTGKTLLTPEYLSPELVHYDGQGGVQLI 327 Query: 324 ASGPDNERRALKNIFFSMITSAEKSVWIATPYFIPDEDILTALRVAAMSGLDVRLLFPAK 383 A GPD + +K+++F+MITSA++S+WIA+PYF+PDEDILTAL++AA+SGLDVR+L P + Sbjct: 328 AGGPDQKWEVIKHLYFAMITSAQRSIWIASPYFVPDEDILTALKIAALSGLDVRILAPKR 387 Query: 384 PDKWLPFLASHSYFPALLESGVKIYEYEKGFIHSKLLITDGEIATIGTANMDMRSFHLNF 443 PDK + F AS SYFP LLE+GVKIYEY KGF+HSK++I DGE+A+IGTANMDMRSFHLNF Sbjct: 388 PDKKIVFYASRSYFPELLEAGVKIYEYSKGFLHSKIMIVDGELASIGTANMDMRSFHLNF 447 Query: 444 EVNALLLQTESVSRIVADFERDLLSTRQIVHETFMNKRLLERVLESAARLMSPLL 498 EVNA L T+S ++VADF DL I +ETF + L RV+ES +RL+SPLL Sbjct: 448 EVNAFLYHTDSTKKLVADFLEDLKEASPIDYETFQQRPLSIRVVESVSRLLSPLL 502 >C3FH06.1 C3FH06_BACTB Cardiolipin synthetase 2 Length = 514 Score = 515 bits (1326), Expect = e-144, Method: Compositional matrix adjust. Identities = 250/480 (52%), Positives = 343/480 (71%), Gaps = 18/480 (3%) Query: 28 IVSVFSTLTVISIGLGIFMENRNPSTTMSWILLLALIPVVGLILYFLFGQNVFKRRKYDK 87 I+S+ T++ IG IF+ENR+PS T++W+++L + PV G Y LFGQN ++R + K Sbjct: 44 IISILFTVSAFLIGCVIFLENRHPSKTLTWLIVLGIFPVFGFFAYLLFGQNFRRKRMFQK 103 Query: 88 KAQRDQMAY------ERIENDALRMHQDWSIFDTTRQKLLGLSQRLARTPISFNSETRIX 141 KA D+ A+ E E LR H+ ++ L L+ RL ISF +ETR Sbjct: 104 KALLDEQAFLQYKGHEDYEERILRNHKH-------QELLFRLADRLGALNISFQTETRTL 156 Query: 142 XXXXXXXXXXXXXXRQAQHHIHMEYYIFRADHIGTRIQQILIEKARAGVAVRFMYDAVGS 201 ++A+HHIHMEYYI R D +GT I+ ILI+K++ GV VRF+YDAVGS Sbjct: 157 TNGDETFQAILNGLKRAKHHIHMEYYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGS 216 Query: 202 IQLSRAFLKELSDAGVQ-VAAYGNSTSFFSSRVNYRNHRKIVVIDGDVGFMGGLNVGDEY 260 +LS+++++EL+DAGV+ + + + ++NYRNHRKIVVIDG+ GF+GGLN+GDEY Sbjct: 217 FKLSKSYIEELNDAGVEMIPFFPVRFPILNDKINYRNHRKIVVIDGNEGFVGGLNIGDEY 276 Query: 261 LSRSKTYGFWRDTHMLLRGEAVRTMQIIFLQDWMHTTGEKILEQDYLSPQLRFTNGD--G 318 L +SK +GFWRDTH+ LRGEAV+++Q+IFLQDW + TGE +L +YL Q + GD G Sbjct: 277 LGKSKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAPEYL--QAKAVEGDHWG 334 Query: 319 AVQIIASGPDNERRALKNIFFSMITSAEKSVWIATPYFIPDEDILTALRVAAMSGLDVRL 378 VQ++A GPDN+ +K+++F+MI SA KS+WIATPYFIPD+DIL+AL+VAA++G+DVRL Sbjct: 335 GVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRL 394 Query: 379 LFPAKPDKWLPFLASHSYFPALLESGVKIYEYEKGFIHSKLLITDGEIATIGTANMDMRS 438 L P+KPDK F AS SYFP LL++GVKIYEYEKGF+HSK++I D ++A+IGTANMDMRS Sbjct: 395 LMPSKPDKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKIVIVDSDLASIGTANMDMRS 454 Query: 439 FHLNFEVNALLLQTESVSRIVADFERDLLSTRQIVHETFMNKRLLERVLESAARLMSPLL 498 FHLNFEVNA L T+S+ R+V DF+ DL + +I + F +RL R++ES RL+SPLL Sbjct: 455 FHLNFEVNAFLYDTDSIKRLVQDFKDDLEESSEIHVDRFHKRRLHRRIVESTYRLLSPLL 514 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,555,928,643 Number of extensions: 99827827 Number of successful extensions: 366366 Number of sequences better than 1.0e-05: 2066 Number of HSP's gapped: 359295 Number of HSP's successfully gapped: 2172 Length of query: 498 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 358 Effective length of database: 1,741,754,103 Effective search space: 623547968874 Effective search space used: 623547968874 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)