BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0164.0007.p (309 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7M5.1 B3K7M5_9BACI Regulatory protein TetR 206 2e-51 Q65F52.1 Q65F52_BACLD YuxN 141 6e-32 C2NR46.1 C2NR46_BACCE Transcriptional regulator, TetR 128 9e-28 C1ER29.1 C1ER29_BACC3 Transcriptional regulator, TetR family 127 1e-27 Q630G8.1 Q630G8_BACCZ Transcriptional regulator, TetR family 125 4e-27 >B3K7M5.1 B3K7M5_9BACI Regulatory protein TetR Length = 317 Score = 206 bits (525), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 123/296 (41%), Positives = 179/296 (60%), Gaps = 5/296 (1%) Query: 3 ADKTTDKKEQIIKTAMQLFAVKGSSSTSMQEIAELCGISKGSLYLVFKSKEELERSIYIY 62 ADKT +K++QI+ TAMQLF+ KGSS TSMQEIAE+CG+SKGSLYL FKSKEELE+SIY + Sbjct: 2 ADKTAEKRQQILITAMQLFSAKGSSVTSMQEIAEVCGMSKGSLYLHFKSKEELEKSIYEH 61 Query: 63 CFRMIRDPLQQEEQNSSRTPREKLRNQIEILLHHVYELREFFQRQF-QELAGKGMTDAPE 121 I+D + + + + P+E+LR Q E+LL H E+REF +Q ++ GK D E Sbjct: 62 IAGRIKDEILRVDHDPLLAPKEQLRKQTEVLLIHFLEIREFLLKQIHDQMPGKPPFDK-E 120 Query: 122 WLRKINGPLLKWSQNKLETLYGKEVLPYTGELFLLGHGMIHSYIFLLFNHESFVSIPRLA 181 +R L+W NKL+T+YG +V PYT E+ + M+ SYI LF E +++ A Sbjct: 121 NMRLEMLAALQWFNNKLKTIYGPDVEPYTIEIGMFMASMLGSYIRFLFMPEFHLNVGITA 180 Query: 182 NHLVDVLDIVVAGLHAGRPAPL--FSSIALESWMENYEAGSHRNPLQLLKEMKERLHAGS 239 +H + +LD +VA + RP PL ++ + + R+PL ++K MK L Sbjct: 181 DHQIQLLDDIVASILRRRPEPLIPMEAVGQLGQLCQTDVPKQRHPLIVIKAMKSELKKVQ 240 Query: 240 GADPHSTEDALESIAILESEILMPHPRKAIILGMIGNLQSYPAVHPQLEDLKKLCS 295 A+P EDA+ES+ I+E E+L P +A++ GM+ NL+ + +LK S Sbjct: 241 -AEPEEREDAMESLNIMEKELLGLQPSRAVLKGMLANLEGIEEIEALYTELKGTIS 295 >Q65F52.1 Q65F52_BACLD YuxN Length = 294 Score = 141 bits (356), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 95/293 (32%), Positives = 156/293 (53%), Gaps = 10/293 (3%) Query: 9 KKEQIIKTAMQLFAVKGSSSTSMQEIAELCGISKGSLYLVFKSKEELERSIYIYCFRMIR 68 K++ II+TA++LFA KG SSTS+QEIA+ C +SKG+ Y+ FKSKE L S+ Y + I Sbjct: 4 KEKVIIETAIKLFAKKGYSSTSVQEIAKECQMSKGAFYIYFKSKEALLLSVLHYYYERIF 63 Query: 69 DPLQQEEQNSSRTPREKLRNQIEILLHHVYELREFFQRQFQELAGKGMTDAPEWLRKING 128 +Q+ + PR+ R Q+ + ++ E +EF QF E + + + +K+ Sbjct: 64 TRIQK-IKTEHDNPRDVYRMQLVVFFENILEHKEFITMQFNERSMPINEEIEAFAKKMRR 122 Query: 129 PLLKWSQNKLETLYGKEVLPYTGELFLLGHGMIHSYI-FLLFNHESFVSIPRLANHLVDV 187 L+ ++ +YGK++ PY+ EL + +GM Y+ ++ H SF + LA+H+++ Sbjct: 123 TTLRLHIENIQNIYGKDIEPYSAELCFMINGMSDVYLELMILLHHSF-DLQSLADHMLNR 181 Query: 188 LDIVVAGLHAGRPAPLFSSIALESWMENYEAGSHRNPLQ--LLKEMKERLHAGSGADPHS 245 LD + G+ PL + W + +PL+ LL EM+ + + P + Sbjct: 182 LDDLAKGMIKRNDQPLIPFEKADEWFGPFYTF---DPLKDALLNEMRVKANKQYNGAPPA 238 Query: 246 TEDALESIAILESEILMPHPRKAIILGMIGNLQSYPAVHPQLEDLKKLCSFHM 298 D ES+ ILE E+ + AII GMI NL+ YPA+ + + L +L M Sbjct: 239 --DLAESLDILEKELRKEKRKNAIIKGMIANLKEYPAIRKETDKLAELLKKDM 289 >C2NR46.1 C2NR46_BACCE Transcriptional regulator, TetR Length = 290 Score = 128 bits (321), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 84/276 (30%), Positives = 148/276 (53%), Gaps = 3/276 (1%) Query: 8 DKKEQIIKTAMQLFAVKGSSSTSMQEIAELCGISKGSLYLVFKSKEELERSIYIYCFRMI 67 +K+ II+ AM+LFA KG ++TS+QEI CGISKG+ YL FKSKEEL + Y + I Sbjct: 3 EKERLIIEMAMKLFATKGVNATSVQEIVTACGISKGAFYLYFKSKEELLLATLRYYYDKI 62 Query: 68 RDPLQQEEQNSSRTPREKLRNQIEILLHHVYELREFFQRQFQELAGKGMTDAPEWLRKIN 127 ++ + E+ S PREK Q+ + + + +EF +E A + E++ ++ Sbjct: 63 QNKMMDIEKESL-LPREKFSKQLHCQFNDIQKHKEFIIMHARENAIPFNKEVEEFMMRMK 121 Query: 128 GPLLKWSQNKLETLYGKEVLPYTGELFLLGHGMIHSYIFLLFNHESFVSIPRLANHLVDV 187 + +N L ++YG +V+PY +L ++ G+ Y+ L+ + + ++ ++ Sbjct: 122 LESHAFYRNSLLSIYGDKVMPYLLDLVIMVEGICRGYLELIILQAPEIDLSYVSAFILKR 181 Query: 188 LDIVVAGLHAGRPAPLFSSIALESWMENYEAGSHRNPLQLLKEMKERLHAGSGADPHSTE 247 +D +V GL A P+ L ++ E + LKE+ + + AD E Sbjct: 182 VDDLVDGLVASSEEPVLHEEKLGEFLCKSELIKEQVKEHFLKEII--VFKRTLADQLEDE 239 Query: 248 DALESIAILESEILMPHPRKAIILGMIGNLQSYPAV 283 + L ++ +LE+E+ M +PR +I GM+ NL++YPA+ Sbjct: 240 ELLVTLDVLEAEMRMQNPRIPVIQGMLFNLEAYPAI 275 >C1ER29.1 C1ER29_BACC3 Transcriptional regulator, TetR family Length = 290 Score = 127 bits (320), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 84/276 (30%), Positives = 148/276 (53%), Gaps = 3/276 (1%) Query: 8 DKKEQIIKTAMQLFAVKGSSSTSMQEIAELCGISKGSLYLVFKSKEELERSIYIYCFRMI 67 +K+ II+ AM+LFA KG ++TS+QEI CGISKG+ YL FKSKEEL + Y + I Sbjct: 3 EKERLIIEMAMKLFATKGVNATSVQEIVTACGISKGAFYLYFKSKEELLLATLRYYYDKI 62 Query: 68 RDPLQQEEQNSSRTPREKLRNQIEILLHHVYELREFFQRQFQELAGKGMTDAPEWLRKIN 127 ++ + E+ S PREK Q+ + + + +EF +E A + E++ ++ Sbjct: 63 QNKMMDIEKESL-LPREKFLKQLHCQFNDIQKHKEFIIMHARENAIPFNKEVEEFMMRMK 121 Query: 128 GPLLKWSQNKLETLYGKEVLPYTGELFLLGHGMIHSYIFLLFNHESFVSIPRLANHLVDV 187 + +N L ++YG +V+PY +L ++ G+ Y+ L+ + + ++ ++ Sbjct: 122 LESHAFYRNSLLSIYGDKVMPYLLDLVIMVEGICRGYLELIILQAPEIDLSYVSAFILKR 181 Query: 188 LDIVVAGLHAGRPAPLFSSIALESWMENYEAGSHRNPLQLLKEMKERLHAGSGADPHSTE 247 +D +V GL A P+ L ++ E + LKE+ + + AD E Sbjct: 182 VDDLVDGLVASSEEPVLHEEKLGEFLCKSELIKEQVKEHFLKEII--VFKRTLADQLEDE 239 Query: 248 DALESIAILESEILMPHPRKAIILGMIGNLQSYPAV 283 + L ++ +LE+E+ M +PR +I GM+ NL++YPA+ Sbjct: 240 ELLVTLDVLEAEMRMKNPRIPVIQGMLFNLEAYPAI 275 >Q630G8.1 Q630G8_BACCZ Transcriptional regulator, TetR family Length = 290 Score = 125 bits (315), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 82/274 (29%), Positives = 145/274 (52%), Gaps = 3/274 (1%) Query: 8 DKKEQIIKTAMQLFAVKGSSSTSMQEIAELCGISKGSLYLVFKSKEELERSIYIYCFRMI 67 +K+ II+ AM+LFA KG ++TS+QEI CGISKG+ YL FKSKEEL + Y + I Sbjct: 3 EKERLIIEMAMKLFATKGVNATSVQEIVTACGISKGAFYLYFKSKEELLLATLRYYYDKI 62 Query: 68 RDPLQQEEQNSSRTPREKLRNQIEILLHHVYELREFFQRQFQELAGKGMTDAPEWLRKIN 127 ++ + E+ S PREK Q+ + + + +EF +E A + E++ ++ Sbjct: 63 QNKMMDIEK-ESLLPREKFAKQLHCQFNDIQKHKEFIIMHARENAIPFNKEVEEFMMRMK 121 Query: 128 GPLLKWSQNKLETLYGKEVLPYTGELFLLGHGMIHSYIFLLFNHESFVSIPRLANHLVDV 187 + +N L ++YG +V+PY +L ++ G+ Y+ L+ + + ++ ++ Sbjct: 122 LESHAFYRNSLLSIYGDKVMPYLLDLVIMVEGICRGYLELIILQAPEIDLSYVSTFILKR 181 Query: 188 LDIVVAGLHAGRPAPLFSSIALESWMENYEAGSHRNPLQLLKEMKERLHAGSGADPHSTE 247 +D +V GL P+ L ++ E + LKE+ + + AD E Sbjct: 182 VDDLVDGLLKSSEEPVLHEEKLGEFLCKSELIKEQVKEHFLKEII--VFKRTLADQLEDE 239 Query: 248 DALESIAILESEILMPHPRKAIILGMIGNLQSYP 281 + L ++ +LE+E+ +P+PR +I GM+ NL+ YP Sbjct: 240 ELLVTLDVLEAEMRLPNPRIPVIQGMLSNLEVYP 273 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,851,532,391 Number of extensions: 78383770 Number of successful extensions: 210259 Number of sequences better than 1.0e-05: 202 Number of HSP's gapped: 210321 Number of HSP's successfully gapped: 202 Length of query: 309 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 174 Effective length of database: 1,788,859,178 Effective search space: 311261496972 Effective search space used: 311261496972 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)