BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0162.0009.p (292 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K465.1 B3K465_9BACI L-serine dehydratase, iron-sulfur-dependen... 418 e-115 C0ZFX6.1 C0ZFX6_BREBN Probable L-serine dehydratase alpha chain ... 251 5e-65 A4IM59.1 A4IM59_GEOTN L-serine dehydratase (Alpha chain) 245 4e-63 B4BJ81.1 B4BJ81_9BACI L-serine dehydratase, iron-sulfur-dependen... 245 4e-63 A1HMG1.1 A1HMG1_9FIRM L-serine dehydratase, iron-sulfur-dependen... 243 2e-62 >B3K465.1 B3K465_9BACI L-serine dehydratase, iron-sulfur-dependent, alpha subunit EC=4.3.1.17 Length = 320 Score = 418 bits (1074), Expect = e-115, Method: Compositional matrix adjust. Identities = 206/292 (70%), Positives = 235/292 (80%) Query: 1 MNFQTLSQLAVLCEERGMGIGALMLEEQSEESGRSKEQEFATMSQYYGVMKEAVHRGMTE 60 M F TL Q+ C E + IG LML EQ +E+G+S+++ F M+ YYG+MKEAVHRG+ E Sbjct: 1 MKFSTLEQIIACCREDQVTIGQLMLNEQIKETGKSRDETFKLMADYYGIMKEAVHRGIHE 60 Query: 61 NTTSRSGLTGLDAQRVASYNAADEPCLGGPAGQAMAYALAVSEVNASMGRIIATPTAGSC 120 S+SGLTG DA RV Y ++ P LG A A+AYAL+VSEVNASMGRI+ATPTAGSC Sbjct: 61 PVPSKSGLTGGDAVRVHQYMSSGVPALGTDACTALAYALSVSEVNASMGRIVATPTAGSC 120 Query: 121 GIIPGVFLSCQERFGWDDDYVVSGLFAAGAIGYVIANNSFVSGAEGGCQAEVGSAIGMAA 180 GIIPGVF+S QERFGW+D+++V GLF+AGAIGYVIANNSF+SGAEGGCQAEVGSAIGMAA Sbjct: 121 GIIPGVFVSTQERFGWEDEHLVMGLFSAGAIGYVIANNSFISGAEGGCQAEVGSAIGMAA 180 Query: 181 GALTELRGGTPAQAVHAVGLALKNTLGLICDPVGGLVEIPCIVRNGFXXXXXXXXXXXXX 240 GALTELRGGTP QAVHAVGLALKN+LGLICDPVGGLVEIPCIVRNGF Sbjct: 181 GALTELRGGTPEQAVHAVGLALKNSLGLICDPVGGLVEIPCIVRNGFGAVTALAASDMAL 240 Query: 241 XXXRSVIPSDEVIKVMLEVGSAMPEKHRETAGGGLAQTPTGRKIMKDLRNKK 292 RSVIPSDEVI VMLEVG+AMP KHRETA GGLAQTPTG+KI+KDL K+ Sbjct: 241 AGVRSVIPSDEVIDVMLEVGTAMPSKHRETAKGGLAQTPTGKKILKDLYGKE 292 >C0ZFX6.1 C0ZFX6_BREBN Probable L-serine dehydratase alpha chain EC=4.3.1.17 Length = 294 Score = 251 bits (641), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 134/282 (47%), Positives = 182/282 (64%) Query: 3 FQTLSQLAVLCEERGMGIGALMLEEQSEESGRSKEQEFATMSQYYGVMKEAVHRGMTENT 62 F+ +++L L E +G I +M+E + E S RS++ M VM++AV RG+TE+ Sbjct: 2 FRNVAELVELAESQGKKISEVMIEAEMEVSQRSRDAIMQDMYANLDVMEKAVRRGLTEDI 61 Query: 63 TSRSGLTGLDAQRVASYNAADEPCLGGPAGQAMAYALAVSEVNASMGRIIATPTAGSCGI 122 S SGLTG DA+++ +Y G A++ ++AV+EVNA+MG I+ATPTAG+CGI Sbjct: 62 RSHSGLTGGDAKKLQTYMETKTFLSGPTLLNAVSMSVAVNEVNAAMGTIVATPTAGACGI 121 Query: 123 IPGVFLSCQERFGWDDDYVVSGLFAAGAIGYVIANNSFVSGAEGGCQAEVGSAIGMAAGA 182 +PG + ++ + +V+ LF AGAIGY IANN+F+SGA GGCQAEVGSA MAA A Sbjct: 122 VPGTLFAVSDKLQPTREEMVNYLFTAGAIGYCIANNAFISGAAGGCQAEVGSATAMAAAA 181 Query: 183 LTELRGGTPAQAVHAVGLALKNTLGLICDPVGGLVEIPCIVRNGFXXXXXXXXXXXXXXX 242 + E+ GGTP ++ AV +ALKN LGL+CDPV GLVE+PC+ RN Sbjct: 182 IVEMAGGTPEESAQAVAIALKNMLGLVCDPVAGLVEVPCVKRNAMGAAIATVAADMAMAG 241 Query: 243 XRSVIPSDEVIKVMLEVGSAMPEKHRETAGGGLAQTPTGRKI 284 +SVIP+DEVI+ M +G AMP +ETA GGLA T TGR I Sbjct: 242 IKSVIPTDEVIEAMYRIGCAMPTTLKETAQGGLAATQTGRMI 283 >A4IM59.1 A4IM59_GEOTN L-serine dehydratase (Alpha chain) Length = 293 Score = 245 bits (625), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 137/282 (48%), Positives = 180/282 (63%), Gaps = 1/282 (0%) Query: 3 FQTLSQLAVLCEERGMGIGALMLEEQSEESGRSKEQEFATMSQYYGVMKEAVHRGMTENT 62 F+ +++L L E+ + I +M+ ++ E SGRS+E+ A M ++ VM+ AV RG+ + Sbjct: 2 FRNVAELVELAEKEQIKIAEVMIRQEIEVSGRSREEIVAQMDRHLEVMERAVERGL-QGV 60 Query: 63 TSRSGLTGLDAQRVASYNAADEPCLGGPAGQAMAYALAVSEVNASMGRIIATPTAGSCGI 122 SRSGLTG DA R+ Y A G A++ A+A +EVNASMG I ATPTAGS G+ Sbjct: 61 VSRSGLTGGDAMRMQRYIEAGRFLSGETILDAVSKAMATNEVNASMGVICATPTAGSAGV 120 Query: 123 IPGVFLSCQERFGWDDDYVVSGLFAAGAIGYVIANNSFVSGAEGGCQAEVGSAIGMAAGA 182 +PG + +E+ ++ LF AGA GYV+ANN+ +SGA GGCQAEVGSA GMAA A Sbjct: 121 VPGTLFAVKEKLQPTRKEMIEFLFTAGAFGYVVANNASISGAAGGCQAEVGSAAGMAAAA 180 Query: 183 LTELRGGTPAQAVHAVGLALKNTLGLICDPVGGLVEIPCIVRNGFXXXXXXXXXXXXXXX 242 L EL GGTP+QA A+ +ALKN LGL+CDPV GLVE+PC+ RN Sbjct: 181 LVELAGGTPSQAAEAMAIALKNMLGLVCDPVAGLVEVPCVKRNAMGAANAMIAADMALAG 240 Query: 243 XRSVIPSDEVIKVMLEVGSAMPEKHRETAGGGLAQTPTGRKI 284 +S IP DEVI+ M +G+AMP +ETA GGLA TP GR I Sbjct: 241 VKSRIPCDEVIEAMYRIGAAMPVALKETAQGGLAATPAGRAI 282 >B4BJ81.1 B4BJ81_9BACI L-serine dehydratase, iron-sulfur-dependent, alpha subunit EC=4.3.1.17 Length = 293 Score = 245 bits (625), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 137/282 (48%), Positives = 180/282 (63%), Gaps = 1/282 (0%) Query: 3 FQTLSQLAVLCEERGMGIGALMLEEQSEESGRSKEQEFATMSQYYGVMKEAVHRGMTENT 62 F+ +++L L E+ + I +M+ ++ E SGRS+E+ A M ++ VM+ AV RG+ + Sbjct: 2 FRNVAELVELAEKEQIKIAEVMIRQEIEVSGRSREEIVAQMDRHLEVMERAVERGL-QGV 60 Query: 63 TSRSGLTGLDAQRVASYNAADEPCLGGPAGQAMAYALAVSEVNASMGRIIATPTAGSCGI 122 SRSGLTG DA R+ Y A G A++ A+A +EVNASMG I ATPTAGS G+ Sbjct: 61 VSRSGLTGGDAMRMQRYIEAGRFLSGETILDAVSKAMATNEVNASMGVICATPTAGSAGV 120 Query: 123 IPGVFLSCQERFGWDDDYVVSGLFAAGAIGYVIANNSFVSGAEGGCQAEVGSAIGMAAGA 182 +PG + +E+ ++ LF AGA GYV+ANN+ +SGA GGCQAEVGSA GMAA A Sbjct: 121 VPGTLFAVKEKLQPTRKEMIEFLFTAGAFGYVVANNASISGAAGGCQAEVGSAAGMAAAA 180 Query: 183 LTELRGGTPAQAVHAVGLALKNTLGLICDPVGGLVEIPCIVRNGFXXXXXXXXXXXXXXX 242 L EL GGTP+QA A+ +ALKN LGL+CDPV GLVE+PC+ RN Sbjct: 181 LVELAGGTPSQAAEAMAIALKNMLGLVCDPVAGLVEVPCVKRNAMGAANAMIAADMALAG 240 Query: 243 XRSVIPSDEVIKVMLEVGSAMPEKHRETAGGGLAQTPTGRKI 284 +S IP DEVI+ M +G+AMP +ETA GGLA TP GR I Sbjct: 241 VKSRIPCDEVIEAMYRIGAAMPVALKETAQGGLAATPAGRAI 282 >A1HMG1.1 A1HMG1_9FIRM L-serine dehydratase, iron-sulfur-dependent, alpha subunit Length = 294 Score = 243 bits (619), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 136/292 (46%), Positives = 176/292 (60%) Query: 1 MNFQTLSQLAVLCEERGMGIGALMLEEQSEESGRSKEQEFATMSQYYGVMKEAVHRGMTE 60 + F +++L L E++ +G ++ + ++E S R ++ + M VM+EAV G+ Sbjct: 2 VRFSRIAELVALAEQQQKPVGYIVWQYETERSQRPSQEIWNQMHLNLAVMREAVQSGLAS 61 Query: 61 NTTSRSGLTGLDAQRVASYNAADEPCLGGPAGQAMAYALAVSEVNASMGRIIATPTAGSC 120 S SGL G DA R+ G +A AYALAVSEVNA MGRI+A PTAGSC Sbjct: 62 REKSLSGLVGGDAARMKDRLQDGSTLCGSAVYKAAAYALAVSEVNAGMGRIVACPTAGSC 121 Query: 121 GIIPGVFLSCQERFGWDDDYVVSGLFAAGAIGYVIANNSFVSGAEGGCQAEVGSAIGMAA 180 GI+PG L+ E G DD+ +V LF IG VIA N+ +SGA+GGCQAE G+A MAA Sbjct: 122 GIVPGALLAVGEGLGCDDETLVQALFTTAGIGLVIAENASISGAKGGCQAECGTAAAMAA 181 Query: 181 GALTELRGGTPAQAVHAVGLALKNTLGLICDPVGGLVEIPCIVRNGFXXXXXXXXXXXXX 240 GA+ E+ GGTP Q HA+ LALKN LGL+CDPV GLVE+PC+ RN F Sbjct: 182 GAIVEMAGGTPRQVGHALALALKNMLGLVCDPVAGLVEVPCVKRNAFGAVHALVAADMAL 241 Query: 241 XXXRSVIPSDEVIKVMLEVGSAMPEKHRETAGGGLAQTPTGRKIMKDLRNKK 292 SVIP+DEVI M ++G MP+ RET+ GGLA+TPT I K L K Sbjct: 242 AGIESVIPADEVIDAMHQIGLVMPKMLRETSEGGLARTPTAVAIEKRLFGKN 293 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,610,307,139 Number of extensions: 63715458 Number of successful extensions: 196235 Number of sequences better than 1.0e-05: 1514 Number of HSP's gapped: 192891 Number of HSP's successfully gapped: 1514 Length of query: 292 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 158 Effective length of database: 1,798,280,193 Effective search space: 284128270494 Effective search space used: 284128270494 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)