BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0162.0005.p (299 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K4K8.1 B3K4K8_9BACI Short-chain dehydrogenase/reductase SDR 423 e-116 Q2BDM2.1 Q2BDM2_9BACI Predicted dehydrogenase; short-chain alcoh... 397 e-109 Q97J63.1 Q97J63_CLOAB Predicted dehydrogenase of short-chain alc... 389 e-106 B1D7Z2.1 B1D7Z2_9BACL Short-chain dehydrogenase/reductase SDR 378 e-103 C1IB72.1 C1IB72_9CLOT Short-chain dehydrogenase/reductase SDR 363 7e-99 >B3K4K8.1 B3K4K8_9BACI Short-chain dehydrogenase/reductase SDR Length = 302 Score = 423 bits (1087), Expect = e-116, Method: Compositional matrix adjust. Identities = 205/302 (67%), Positives = 239/302 (79%), Gaps = 3/302 (0%) Query: 1 MNPV--YPYYGERTVCRVQPISFPPQHQDRQPGLESLMHPRPISENPDSRGSGKLDGKVA 58 MNP PY G +T C+ QPI+FPPQHQ+ QPGLESLM PRPISE+P RGSGKLDGK+A Sbjct: 1 MNPQQPIPYVGSKTECKEQPIAFPPQHQNCQPGLESLMSPRPISEDPAYRGSGKLDGKIA 60 Query: 59 LITGGDSXXXXXXXXXXXXXXXDVAIAYLYEASDAEATQRRIEELGQRCLRIQTDLRCKA 118 ++TGGDS D+ I YLYE SDAE T++RIE LG+RCL ++ DLR K Sbjct: 61 IVTGGDSGIGKAAAIAFAKEGADLVIPYLYETSDAEVTKQRIESLGRRCLLMKIDLRYKK 120 Query: 119 NCTQAVEHTVRTFGKLDILVNNHGVQYPQQSLLDISEEQLYETYRTNIFPFFFLTQAALP 178 NC V T+ FG++D+LVNNH VQY Q+S+LDI+EEQL+ TY+TNIFPFFF+ QAALP Sbjct: 121 NCEAVVNETIACFGRVDVLVNNHAVQYVQRSILDITEEQLHHTYQTNIFPFFFMIQAALP 180 Query: 179 HLRTGASIINTASITAYQGHKELIDYSSTKGAIVTFTRSLALSLADSGIRVNSVAPGPVW 238 H++ GASIINTASITAY G KELIDYSSTKGAIV+ TRSLALSL D GIRVNSVAPG VW Sbjct: 181 HMKAGASIINTASITAYDGLKELIDYSSTKGAIVSLTRSLALSLVDQGIRVNSVAPGSVW 240 Query: 239 TPLIPSSYSAEEVSVFGTDTPFKRAAQPYELAGAYVYLAGP-DSTYVTGTCIHVNGGDMV 297 TPLIPSS+SA+EV+VFGT++P KRAAQPYELA AYVYLA S++VTG IHVNGG MV Sbjct: 241 TPLIPSSFSADEVAVFGTNSPMKRAAQPYELAPAYVYLASSITSSFVTGQAIHVNGGQMV 300 Query: 298 TS 299 TS Sbjct: 301 TS 302 >Q2BDM2.1 Q2BDM2_9BACI Predicted dehydrogenase; short-chain alcohol dehydrogenase family protein Length = 299 Score = 397 bits (1019), Expect = e-109, Method: Compositional matrix adjust. Identities = 187/299 (62%), Positives = 229/299 (76%) Query: 1 MNPVYPYYGERTVCRVQPISFPPQHQDRQPGLESLMHPRPISENPDSRGSGKLDGKVALI 60 M PVYPYYG++ C+ +P+SFPPQHQD QPGLE M PRPISE+PD RGSGKL GKVA+I Sbjct: 1 MYPVYPYYGKKEECKEKPLSFPPQHQDVQPGLEYQMVPRPISEDPDYRGSGKLKGKVAII 60 Query: 61 TGGDSXXXXXXXXXXXXXXXDVAIAYLYEASDAEATQRRIEELGQRCLRIQTDLRCKANC 120 TGGDS V I YL E DA TQ RI ELG L I D+R + Sbjct: 61 TGGDSGIGRAAAYAFVKEGAKVVIVYLNEHQDARETQERIRELGGESLFIAGDMRREDFS 120 Query: 121 TQAVEHTVRTFGKLDILVNNHGVQYPQQSLLDISEEQLYETYRTNIFPFFFLTQAALPHL 180 + VE T+ FG++DILVNNHGVQ+ Q+S+LDI+ EQL++T+ TNIF FF+LT+A LPH+ Sbjct: 121 REIVEKTLHHFGRIDILVNNHGVQFVQKSILDITREQLFDTFETNIFAFFYLTKAVLPHM 180 Query: 181 RTGASIINTASITAYQGHKELIDYSSTKGAIVTFTRSLALSLADSGIRVNSVAPGPVWTP 240 + G SIINT S+TAY+GHK+LIDYSSTKGAIVTFTRSL+LSL +GIRVN++APGP+WTP Sbjct: 181 KAGGSIINTTSVTAYEGHKDLIDYSSTKGAIVTFTRSLSLSLEKNGIRVNAIAPGPIWTP 240 Query: 241 LIPSSYSAEEVSVFGTDTPFKRAAQPYELAGAYVYLAGPDSTYVTGTCIHVNGGDMVTS 299 LIP+S+SA++VS FGTDTP KRA QP+ELA AYVYLA DS Y++G +H+NGG MV+S Sbjct: 241 LIPASFSAKKVSEFGTDTPMKRAGQPFELAPAYVYLASDDSRYMSGQVLHINGGTMVSS 299 >Q97J63.1 Q97J63_CLOAB Predicted dehydrogenase of short-chain alcohol dehydrogenase family, ortholog of YHDF B.subtilis Length = 299 Score = 389 bits (998), Expect = e-106, Method: Compositional matrix adjust. Identities = 188/294 (63%), Positives = 222/294 (75%) Query: 6 PYYGERTVCRVQPISFPPQHQDRQPGLESLMHPRPISENPDSRGSGKLDGKVALITGGDS 65 PY+ E C V P +FPPQHQ+RQPG+ES+M PRPI NP SGKL KVALITGGDS Sbjct: 6 PYFRETNNCPVPPTNFPPQHQNRQPGIESVMRPRPIYNNPCYYASGKLRNKVALITGGDS 65 Query: 66 XXXXXXXXXXXXXXXDVAIAYLYEASDAEATQRRIEELGQRCLRIQTDLRCKANCTQAVE 125 DVAI YLYE +DA T+R IE LGQRCL I+ D+R C +AVE Sbjct: 66 GIGRAAAVAFAKEKADVAIVYLYERNDAMETKRVIESLGQRCLTIEGDVRRDGFCREAVE 125 Query: 126 HTVRTFGKLDILVNNHGVQYPQQSLLDISEEQLYETYRTNIFPFFFLTQAALPHLRTGAS 185 T+RTFG L+ILVNN VQ+PQ S+L+I+ EQL T+RTNIF F++T+AALP+L+ G+S Sbjct: 126 RTIRTFGNLNILVNNAAVQFPQDSILNITNEQLENTFRTNIFSMFYITKAALPYLKYGSS 185 Query: 186 IINTASITAYQGHKELIDYSSTKGAIVTFTRSLALSLADSGIRVNSVAPGPVWTPLIPSS 245 IINTASITAY+G K LIDYSSTKGAIV+FTRSL+ SL D GIRVN+VAPGP+WTPLIP+S Sbjct: 186 IINTASITAYEGEKTLIDYSSTKGAIVSFTRSLSKSLVDKGIRVNAVAPGPIWTPLIPAS 245 Query: 246 YSAEEVSVFGTDTPFKRAAQPYELAGAYVYLAGPDSTYVTGTCIHVNGGDMVTS 299 +SA V+ FG DTP KRA QPYELA AYVYLA DS+YVTG IH+NGG MVT+ Sbjct: 246 FSAARVATFGKDTPMKRAGQPYELAPAYVYLASDDSSYVTGEVIHINGGRMVTT 299 >B1D7Z2.1 B1D7Z2_9BACL Short-chain dehydrogenase/reductase SDR Length = 299 Score = 378 bits (970), Expect = e-103, Method: Compositional matrix adjust. Identities = 182/296 (61%), Positives = 222/296 (75%) Query: 1 MNPVYPYYGERTVCRVQPISFPPQHQDRQPGLESLMHPRPISENPDSRGSGKLDGKVALI 60 M P+YPYY + T C+ P++FPPQHQDRQPGLE LM+P PIS+NP +GSGKL GKVA+I Sbjct: 1 MIPIYPYYSQETKCQQVPVTFPPQHQDRQPGLEYLMNPTPISDNPGYKGSGKLKGKVAII 60 Query: 61 TGGDSXXXXXXXXXXXXXXXDVAIAYLYEASDAEATQRRIEELGQRCLRIQTDLRCKANC 120 TGGDS DVAI YLYE DA AT++ +E+ G RCL I+ DLR Sbjct: 61 TGGDSGIGRAVAIGFSKEGADVAIVYLYEREDALATKQMVEQYGGRCLLIEGDLRKPEFS 120 Query: 121 TQAVEHTVRTFGKLDILVNNHGVQYPQQSLLDISEEQLYETYRTNIFPFFFLTQAALPHL 180 ++ V+ T+ +G LDILV N GVQ+PQ+ +LDIS EQL +TYRTNIFP F+LT+AALPHL Sbjct: 121 SEVVQQTIECYGCLDILVLNQGVQFPQKCILDISNEQLEDTYRTNIFPHFYLTKAALPHL 180 Query: 181 RTGASIINTASITAYQGHKELIDYSSTKGAIVTFTRSLALSLADSGIRVNSVAPGPVWTP 240 + G+SII+TAS+TAY G L+DYSSTKGAIV+FTRSL+L L GIRVN+VAPGP+WTP Sbjct: 181 KAGSSIISTASVTAYAGAPLLVDYSSTKGAIVSFTRSLSLQLVGCGIRVNAVAPGPIWTP 240 Query: 241 LIPSSYSAEEVSVFGTDTPFKRAAQPYELAGAYVYLAGPDSTYVTGTCIHVNGGDM 296 LI SSYSAE V FG +TP KRA QP+ELA Y+YLA DS++VTG +HVNGG M Sbjct: 241 LIVSSYSAEYVKTFGLETPMKRAGQPFELAPTYIYLASDDSSFVTGQVLHVNGGVM 296 >C1IB72.1 C1IB72_9CLOT Short-chain dehydrogenase/reductase SDR Length = 296 Score = 363 bits (933), Expect = 7e-99, Method: Compositional matrix adjust. Identities = 171/280 (61%), Positives = 209/280 (74%) Query: 20 SFPPQHQDRQPGLESLMHPRPISENPDSRGSGKLDGKVALITGGDSXXXXXXXXXXXXXX 79 SFPPQHQD QPG+ES+M+PRPI +NP+ G GKL KVA+ITGGDS Sbjct: 17 SFPPQHQDVQPGIESIMNPRPIFDNPNYMGCGKLKDKVAIITGGDSGIGRATAVAFAKEG 76 Query: 80 XDVAIAYLYEASDAEATQRRIEELGQRCLRIQTDLRCKANCTQAVEHTVRTFGKLDILVN 139 + I YLYE DAE T+ IE+ G CL ++ D+ ++ C + VE T+ FGK+DILVN Sbjct: 77 AKIVIVYLYEKEDAEETKEYIEKYGSECLNLEGDISEESFCKEIVERTIERFGKVDILVN 136 Query: 140 NHGVQYPQQSLLDISEEQLYETYRTNIFPFFFLTQAALPHLRTGASIINTASITAYQGHK 199 N GVQ+PQ+S+ DI+ EQL T++ N+F F+LT+AALP+L+ G SIINT S+TAYQGHK Sbjct: 137 NAGVQFPQKSIEDITAEQLELTFKVNVFSMFYLTKAALPYLKRGGSIINTTSVTAYQGHK 196 Query: 200 ELIDYSSTKGAIVTFTRSLALSLADSGIRVNSVAPGPVWTPLIPSSYSAEEVSVFGTDTP 259 ELIDYS+TKGAI FTRSL+ SL D GIRVN VAPGP+WTPLI SS+S+EEV+ FG+DTP Sbjct: 197 ELIDYSATKGAITAFTRSLSQSLVDKGIRVNGVAPGPIWTPLIVSSFSSEEVATFGSDTP 256 Query: 260 FKRAAQPYELAGAYVYLAGPDSTYVTGTCIHVNGGDMVTS 299 KRA +PYELA AYVYLA DS+YVTG IHVNGG MV S Sbjct: 257 MKRAGEPYELAPAYVYLASDDSSYVTGQVIHVNGGTMVES 296 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,722,852,434 Number of extensions: 65419284 Number of successful extensions: 223759 Number of sequences better than 1.0e-05: 22974 Number of HSP's gapped: 193720 Number of HSP's successfully gapped: 23017 Length of query: 299 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 164 Effective length of database: 1,788,859,178 Effective search space: 293372905192 Effective search space used: 293372905192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)