BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0162.0003.p (188 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K4K6.1 B3K4K6_9BACI Carbohydrate kinase, YjeF related protein 197 3e-49 B0TE80.1 B0TE80_HELMI Yjef family protein 137 3e-31 Q2RGI2.1 Q2RGI2_MOOTA Putative uncharacterized protein 133 6e-30 A8ZVC4.1 A8ZVC4_DESOH Carbohydrate kinase, YjeF related protein 129 2e-28 A3DIW6.1 A3DIW6_CLOTH Carbohydrate kinase, YjeF related protein 124 5e-27 >B3K4K6.1 B3K4K6_9BACI Carbohydrate kinase, YjeF related protein Length = 582 Score = 197 bits (502), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 105/189 (55%), Positives = 123/189 (65%), Gaps = 1/189 (0%) Query: 1 VLRLSTGRDVTAVGPGLGRFAGDQEWLRALWEGIAAPMVIDADALNIL-ADSDYSSWTSR 59 VL L+ RDV AVGPGLGRF GD WLR LWE P+V+DADALN+L A D S+W R Sbjct: 394 VLALAKSRDVLAVGPGLGRFEGDARWLRRLWEEWEGPLVVDADALNMLSAAGDLSAWKPR 453 Query: 60 CHPVILTPHPGEMARLAGISTAEVQRDRIGLALAYAREHRVTLVLKGAHTVVATPEGEAY 119 +LTPHPGEM+RL GI TAE+QRDRIG A +A H VTLVLKGAHTVVA+P+G+ Y Sbjct: 454 AAATVLTPHPGEMSRLLGIPTAELQRDRIGAAAGFATGHGVTLVLKGAHTVVASPDGQTY 513 Query: 120 VNITXXXXXXXXXXXDVLTXXXXXXXXXXXXXXXXXXFGVYLHGLAGERAARQRHDPSAL 179 +N T DVLT GV+LHG AGERAAR R P+++ Sbjct: 514 INTTGTSGMATGGTGDVLTGIIAGLLAQGHAGPSAAALGVWLHGRAGERAARHRGHPASV 573 Query: 180 IAGDIIEAL 188 IAGD+IEAL Sbjct: 574 IAGDVIEAL 582 >B0TE80.1 B0TE80_HELMI Yjef family protein Length = 515 Score = 137 bits (346), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 85/178 (47%), Positives = 100/178 (56%), Gaps = 2/178 (1%) Query: 11 TAVGPGLGRFAGDQEWLRALWEGIAAPMVIDADALNILADSDYSSWTSRCHPVILTPHPG 70 T VGPGLGR A ++L A EG P+VIDAD LN LAD + + PVILTPHPG Sbjct: 330 TVVGPGLGRAAERADFLLACLEGAVGPVVIDADGLNALAD-NIDLLAAAKGPVILTPHPG 388 Query: 71 EMARLAGISTAEVQRDRIGLALAYAREHRVTLVLKGAHTVVATPEGEAYVNITXXXXXXX 130 EMARLAG T VQ DR G+A YARE +VLKGA T+V TP+G+ YVN T Sbjct: 389 EMARLAGCDTVAVQSDRAGVATRYAREWGCIIVLKGARTLVVTPDGQVYVNPTGNPGMAA 448 Query: 131 XXXXDVLTXXXXXXXXXXXXXXXXXXFGVYLHGLAGERAARQRHDPSALIAGDIIEAL 188 DVL GVY+HGLAG+ +AR R A+ AGDI +L Sbjct: 449 GGSGDVLAGIIGALAAQGFAAERAAALGVYIHGLAGDCSAR-RLGQRAMSAGDIAGSL 505 >Q2RGI2.1 Q2RGI2_MOOTA Putative uncharacterized protein Length = 530 Score = 133 bits (335), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 77/180 (42%), Positives = 103/180 (57%), Gaps = 2/180 (1%) Query: 9 DVTAVGPGLGRFAGDQEWLRALWEGIAAPMVIDADALNILADSDYSSWTSRCHPVILTPH 68 DV A+GPGL R + ++ L + P V+DADALN LA +D T P++LTPH Sbjct: 331 DVLAIGPGLSRDPATVDLVKELLPRVQVPAVVDADALNALA-TDTRVLTGDHGPLVLTPH 389 Query: 69 PGEMARLAGISTAEVQRDRIGLALAYAREHRVTLVLKGAHTVVATPEGEAYVNITXXXXX 128 PGEMARL G + A++Q DR+ +A YARE + L+LKGA TV+A P+G+ Y+N T Sbjct: 390 PGEMARLLGTTAAKIQEDRLEIAAKYAREWQAVLLLKGARTVIAWPDGQVYINPTGNPGM 449 Query: 129 XXXXXXDVLTXXXXXXXXXXXXXXXXXXFGVYLHGLAGERAARQRHDPSALIAGDIIEAL 188 DVLT G YLHG AG+ AARQR A++AGD+++ L Sbjct: 450 ATAGSGDVLTGIIAGLAGQGLKPGVAAALGAYLHGAAGDEAARQRGQ-RAMMAGDLLDFL 508 >A8ZVC4.1 A8ZVC4_DESOH Carbohydrate kinase, YjeF related protein Length = 533 Score = 129 bits (323), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 78/185 (42%), Positives = 100/185 (54%), Gaps = 4/185 (2%) Query: 1 VLRLSTGRDVTAVGPGLGRFAGDQEWLRALWEGIAAPMVIDADALNILADS-DYSSWTSR 59 +L +TG+ A+GPG+G + LR L E APMVIDAD L LAD D S++ Sbjct: 322 ILEQTTGKKALAIGPGMGTGQAARNLLRGLMEKSDAPMVIDADGLTCLADDPDLLSFSR- 380 Query: 60 CHPVILTPHPGEMARLAGISTAEVQRDRIGLALAYAREHRVTLVLKGAHTVVATPEGEAY 119 + TPHPGEMARL G++ A+VQ DRI A +AR H V LVLKGA TV+A P+G + Sbjct: 381 -DRAVFTPHPGEMARLCGMTVAQVQNDRIRCAQDFARTHHVHLVLKGAGTVIAHPDGTVF 439 Query: 120 VNITXXXXXXXXXXXDVLTXXXXXXXXXXXXXXXXXXFGVYLHGLAGERAARQRHDPSAL 179 VN T DVLT GV+LHGLAG+ A + + L Sbjct: 440 VNRTGNPGMASGGMGDVLTGVIAGLFCQGYETGIAARLGVFLHGLAGDIAGEETGK-TGL 498 Query: 180 IAGDI 184 +A D+ Sbjct: 499 LASDV 503 >A3DIW6.1 A3DIW6_CLOTH Carbohydrate kinase, YjeF related protein Length = 515 Score = 124 bits (310), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 71/180 (39%), Positives = 100/180 (55%), Gaps = 2/180 (1%) Query: 9 DVTAVGPGLGRFAGDQEWLRALWEGIAAPMVIDADALNILADSDYSSWTSRCHPVILTPH 68 D A+GPGL ++ + ++ E PMVIDAD LN+++ + +R PV+LTPH Sbjct: 330 DAVAIGPGLSTKEDVEDVVFSVVENCKVPMVIDADGLNLISRNLPVLKKARA-PVVLTPH 388 Query: 69 PGEMARLAGISTAEVQRDRIGLALAYAREHRVTLVLKGAHTVVATPEGEAYVNITXXXXX 128 PGEMARL G+S E+Q+ R+G A ++ + VT VLKGA TVVA+P+G ++N T Sbjct: 389 PGEMARLTGLSIGEIQKRRVGTAREFSEKWGVTTVLKGAKTVVASPDGRVFINPTGNSGM 448 Query: 129 XXXXXXDVLTXXXXXXXXXXXXXXXXXXFGVYLHGLAGERAARQRHDPSALIAGDIIEAL 188 DVLT GVYLHGL G+R A + + L+AGD+ E + Sbjct: 449 STGGTGDVLTGIIASFIGQGLDPVDAAVAGVYLHGLCGDRVANVKGE-HGLVAGDLAEEI 507 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 968,647,626 Number of extensions: 32034475 Number of successful extensions: 78966 Number of sequences better than 1.0e-05: 903 Number of HSP's gapped: 77688 Number of HSP's successfully gapped: 903 Length of query: 188 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 60 Effective length of database: 1,854,806,283 Effective search space: 111288376980 Effective search space used: 111288376980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)