BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0045.p (408 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBJ9.1 B3KBJ9_9BACI Cysteine desulfurase EC=2.8.1.7 671 0.0 B1DKG8.1 B1DKG8_9BACL Cysteine desulfurase EC=2.8.1.7 633 e-180 Q8ENU8.1 Q8ENU8_OCEIH Cysteine desulfurase EC=2.8.1.7 618 e-175 B7GF74.1 B7GF74_ANOFW Cysteine desulfurase EC=2.8.1.7 612 e-173 C2WUQ6.1 C2WUQ6_BACCE Cysteine desulfurase 612 e-173 >B3KBJ9.1 B3KBJ9_9BACI Cysteine desulfurase EC=2.8.1.7 Length = 406 Score = 671 bits (1731), Expect = 0.0, Method: Compositional matrix adjust. Identities = 311/404 (76%), Positives = 357/404 (88%), Gaps = 1/404 (0%) Query: 1 MISSAIREQFPILNQTINGHPLVYLDSAATSQKPRQVIEAVKSYYEWDNSNVHRGVHTLG 60 MI+S +REQFPIL+Q INGHPLVYLD+AATSQKP+ VI+AVK YYEW+NSNVHRGVHTLG Sbjct: 1 MIAS-VREQFPILHQEINGHPLVYLDNAATSQKPQVVIDAVKRYYEWENSNVHRGVHTLG 59 Query: 61 SRATDAYEGAREKLAKFINARSTKEIIFTRGTTTALNIVASSYGPGNVGEGDEIVITQME 120 SRATDAYEGAREK+A+FINA S+++IIFTRGTTTALN+VASSY G GDEIV+T+ME Sbjct: 60 SRATDAYEGAREKVARFINAESSEQIIFTRGTTTALNLVASSYARSVCGPGDEIVLTEME 119 Query: 121 HHSNFIPWQQLAKKTGATLKFIPLQKDGSITLEDAEQTITDKTKIVAIAYVSNVMGISHP 180 HHSN IPWQQ A GATLK+IPLQ DG+I +ED E +T++TK+VAIAYVSNVMG+ HP Sbjct: 120 HHSNLIPWQQAAIAVGATLKYIPLQPDGTIRVEDVEALVTERTKVVAIAYVSNVMGVVHP 179 Query: 181 VKELAAIAHRHGAVIVVDGAQSTPHMKVDVQDLDCDFYALSGHKMLAPTGIGALYGKKAL 240 VK++A IAHRHGAVIVVDGAQSTPHMKVDVQDLDCDFYALSGHKM APTGIGALYGKKAL Sbjct: 180 VKQIAEIAHRHGAVIVVDGAQSTPHMKVDVQDLDCDFYALSGHKMCAPTGIGALYGKKAL 239 Query: 241 LEAMEPVEFGGEMIDDVGLYDSTWKELPWKFEGGTPXXXXXXXXXXXXDFLQEIGLDEIH 300 LE+MEP+EFGGEMI+DVGLY+S WKELPWKFEGGTP DFL+EIG+D I Sbjct: 240 LESMEPIEFGGEMINDVGLYESNWKELPWKFEGGTPIIAGAVGLGAAIDFLEEIGMDAIE 299 Query: 301 RHEMELAAYAEQRLSEVDGLTIYGPRNRKVGVVTFNLGDVHPHDVATVLDAEGVAVRAGH 360 RHE +LA YA R+SE++G++IYGPR RKVG++TFNLGDVHPHDVATVLDA+G+A+RAGH Sbjct: 300 RHEAQLANYAMDRISEIEGISIYGPRKRKVGLITFNLGDVHPHDVATVLDAQGIAIRAGH 359 Query: 361 HCCQPLMRWLQVSSTARASFYLYNSEQDVDALVQALIKAKEYFG 404 HCCQPLMRWL+VSSTARASFYLYN+E+DVD LV ALI+ KEYFG Sbjct: 360 HCCQPLMRWLKVSSTARASFYLYNTEEDVDRLVSALIQTKEYFG 403 >B1DKG8.1 B1DKG8_9BACL Cysteine desulfurase EC=2.8.1.7 Length = 408 Score = 633 bits (1633), Expect = e-180, Method: Compositional matrix adjust. Identities = 290/404 (71%), Positives = 345/404 (85%) Query: 5 AIREQFPILNQTINGHPLVYLDSAATSQKPRQVIEAVKSYYEWDNSNVHRGVHTLGSRAT 64 A+REQFPIL+Q +NGHPLVYLDSAA+SQKPR VI+AV YYE D+SNVHRGVHTLGSRAT Sbjct: 5 AVREQFPILHQQVNGHPLVYLDSAASSQKPRSVIDAVSRYYELDHSNVHRGVHTLGSRAT 64 Query: 65 DAYEGAREKLAKFINARSTKEIIFTRGTTTALNIVASSYGPGNVGEGDEIVITQMEHHSN 124 DAYEGAREK+AKF+NA+S +EI+FTRGTTTALN+VASSY EGDEIVI QMEHH+N Sbjct: 65 DAYEGAREKVAKFLNAKSAEEIVFTRGTTTALNLVASSYARSVCKEGDEIVINQMEHHAN 124 Query: 125 FIPWQQLAKKTGATLKFIPLQKDGSITLEDAEQTITDKTKIVAIAYVSNVMGISHPVKEL 184 FIPWQQ AK TGATLKFIPLQ DG+I+L DAEQTIT TK+VA+ YVSNV+G+ +P+KE+ Sbjct: 125 FIPWQQAAKATGATLKFIPLQPDGTISLADAEQTITPNTKVVAMTYVSNVLGVVNPIKEI 184 Query: 185 AAIAHRHGAVIVVDGAQSTPHMKVDVQDLDCDFYALSGHKMLAPTGIGALYGKKALLEAM 244 AIAHR+GAV+VVDGAQSTPHMKVDVQDLDCDFY LSGHKM PTGIGALYGKK LL M Sbjct: 185 TAIAHRNGAVMVVDGAQSTPHMKVDVQDLDCDFYGLSGHKMCGPTGIGALYGKKELLSNM 244 Query: 245 EPVEFGGEMIDDVGLYDSTWKELPWKFEGGTPXXXXXXXXXXXXDFLQEIGLDEIHRHEM 304 EP+E+GGEMID V LY+STWKE P++FEGGTP DFL++IG+DEI +HE Sbjct: 245 EPIEYGGEMIDFVDLYESTWKEAPYRFEGGTPIIAGAVGLGAAIDFLEQIGIDEIDKHER 304 Query: 305 ELAAYAEQRLSEVDGLTIYGPRNRKVGVVTFNLGDVHPHDVATVLDAEGVAVRAGHHCCQ 364 ++AAYA +LS +D +TIYGP+ +VG+VTFNLGD+HPHDV+TVLD+ G+A+RAGHHCCQ Sbjct: 305 KMAAYAYDKLSTIDDITIYGPKANRVGLVTFNLGDIHPHDVSTVLDSYGIAIRAGHHCCQ 364 Query: 365 PLMRWLQVSSTARASFYLYNSEQDVDALVQALIKAKEYFGYELG 408 PLMRWL VS+TARASFYLYN+E+++D L + LI+ KE+FG +G Sbjct: 365 PLMRWLNVSATARASFYLYNNEEEIDRLAEGLIRTKEFFGDAIG 408 >Q8ENU8.1 Q8ENU8_OCEIH Cysteine desulfurase EC=2.8.1.7 Length = 407 Score = 618 bits (1593), Expect = e-175, Method: Compositional matrix adjust. Identities = 290/404 (71%), Positives = 340/404 (84%), Gaps = 1/404 (0%) Query: 1 MISSAIREQFPILNQTINGHPLVYLDSAATSQKPRQVIEAVKSYYEWDNSNVHRGVHTLG 60 M +AI++QFPILNQ +NGHPLVYLDS+ATSQKP VIEAV +YY +NSNVHRGVHTLG Sbjct: 1 MDVNAIKQQFPILNQEVNGHPLVYLDSSATSQKPLSVIEAVNAYYRENNSNVHRGVHTLG 60 Query: 61 SRATDAYEGAREKLAKFINARSTKEIIFTRGTTTALNIVASSYGPGNVGEGDEIVITQME 120 +RATD YEG+REK+ FINA STKEIIFTRGTTT++NIVASSY NVG GDEIVITQME Sbjct: 61 TRATDQYEGSREKVRSFINANSTKEIIFTRGTTTSINIVASSYARMNVGPGDEIVITQME 120 Query: 121 HHSNFIPWQQLAKKTGATLKFIPLQKDGSITLEDAEQTITDKTKIVAIAYVSNVMGISHP 180 HHSN IPWQQ+AK TGA+L ++PLQ+DG+IT+EDA++TITD+TKIVA+A+VSNV+G +P Sbjct: 121 HHSNLIPWQQVAKATGASLVYLPLQEDGTITIEDAKKTITDQTKIVAMAHVSNVLGSINP 180 Query: 181 VKELAAIAHRHGAVIVVDGAQSTPHMKVDVQDLDCDFYALSGHKMLAPTGIGALYGKKAL 240 +KE+AAIAH+H A++VVDGAQ PHMKVDV+DLDCDFYA SGHKM PTGIG LYGK+ L Sbjct: 181 IKEIAAIAHQHDAIMVVDGAQGAPHMKVDVEDLDCDFYAFSGHKMCGPTGIGVLYGKQHL 240 Query: 241 LEAMEPVEFGGEMIDDVGLYDSTWKELPWKFEGGTPXXXXXXXXXXXXDFLQEIGLDEIH 300 LE MEP+EFGGEMID V LYDSTWKELPWKFEGGTP DFL+ IG EI Sbjct: 241 LENMEPIEFGGEMIDFVNLYDSTWKELPWKFEGGTPIIAGAIGLGAAIDFLENIGYQEIV 300 Query: 301 RHEMELAAYAEQRLSEVDGLTIYGPRNRKVGVVTFNLGDVHPHDVATVLDAEGVAVRAGH 360 HE ELAA+A +++ +DG++IYGP R G+VTFNL DVHPHD ATVLDAEG+AVRAGH Sbjct: 301 NHENELAAHALKQMRTIDGISIYGPEQR-AGLVTFNLDDVHPHDTATVLDAEGIAVRAGH 359 Query: 361 HCCQPLMRWLQVSSTARASFYLYNSEQDVDALVQALIKAKEYFG 404 HC QPLM+WL VS+TARASFYLYNS++DVD LV+ L+K KEYFG Sbjct: 360 HCAQPLMKWLNVSATARASFYLYNSKEDVDRLVEGLLKTKEYFG 403 >B7GF74.1 B7GF74_ANOFW Cysteine desulfurase EC=2.8.1.7 Length = 407 Score = 612 bits (1578), Expect = e-173, Method: Compositional matrix adjust. Identities = 289/400 (72%), Positives = 336/400 (84%), Gaps = 1/400 (0%) Query: 6 IREQFPILNQTINGHPLVYLDSAATSQKPRQVIEAVKSYYEWDNSNVHRGVHTLGSRATD 65 +R+ FPIL+Q +NG PLVYLDSAATSQKP VIEA+ +YY NSNVHRGVHTLG++ATD Sbjct: 7 LRQLFPILDQQVNGKPLVYLDSAATSQKPLPVIEAIDNYYRQYNSNVHRGVHTLGTKATD 66 Query: 66 AYEGAREKLAKFINARSTKEIIFTRGTTTALNIVASSYGPGNVGEGDEIVITQMEHHSNF 125 AYEGAREK+ +FI A ST+EIIFTRGTTTA+N+VA+SYG NV EGDEIVIT MEHHSN Sbjct: 67 AYEGAREKVRRFIGAASTEEIIFTRGTTTAINLVAASYGRANVREGDEIVITYMEHHSNI 126 Query: 126 IPWQQLAKKTGATLKFIPLQKDGSITLEDAEQTITDKTKIVAIAYVSNVMGISHPVKELA 185 IPWQQ+AK+TGATLK+IPLQ DG+I LED EQT+T TKIVA+ +VSNV+G +PVKE+A Sbjct: 127 IPWQQVAKQTGATLKYIPLQPDGTIRLEDVEQTVTPNTKIVAVMHVSNVLGTINPVKEIA 186 Query: 186 AIAHRHGAVIVVDGAQSTPHMKVDVQDLDCDFYALSGHKMLAPTGIGALYGKKALLEAME 245 IAH++GAV+VVDGAQSTPHMKVDVQD+DCDFYA SGHKM PTGIG LYGKK LLE ME Sbjct: 187 QIAHKNGAVVVVDGAQSTPHMKVDVQDIDCDFYAFSGHKMCGPTGIGVLYGKKRLLEQME 246 Query: 246 PVEFGGEMIDDVGLYDSTWKELPWKFEGGTPXXXXXXXXXXXXDFLQEIGLDEIHRHEME 305 PVEFGGEMID VGLY+STWKELPWKFEGGTP DFL+++GLD I HE Sbjct: 247 PVEFGGEMIDFVGLYESTWKELPWKFEGGTPIIAGAIGLGAAIDFLEQVGLDHIAEHEHR 306 Query: 306 LAAYAEQRLSEVDGLTIYGPRNRKVGVVTFNLGDVHPHDVATVLDAEGVAVRAGHHCCQP 365 LA YA ++L+ +DGLTIYGP+ R G+VTFN+ VHPHDVATVLDAEG+AVRAGHHC QP Sbjct: 307 LAQYALEQLATIDGLTIYGPKAR-AGLVTFNIDGVHPHDVATVLDAEGIAVRAGHHCAQP 365 Query: 366 LMRWLQVSSTARASFYLYNSEQDVDALVQALIKAKEYFGY 405 LM+WL V++TARASFYLYN+E+++DALV AL K KEYF Y Sbjct: 366 LMKWLNVTATARASFYLYNTEEEIDALVSALKKTKEYFSY 405 >C2WUQ6.1 C2WUQ6_BACCE Cysteine desulfurase Length = 406 Score = 612 bits (1577), Expect = e-173, Method: Compositional matrix adjust. Identities = 289/398 (72%), Positives = 334/398 (83%), Gaps = 1/398 (0%) Query: 6 IREQFPILNQTINGHPLVYLDSAATSQKPRQVIEAVKSYYEWDNSNVHRGVHTLGSRATD 65 IR+QFPIL+Q +NG LVY DSAATSQKP QVIE ++ YY+ NSNVHRGVHTLG++ATD Sbjct: 6 IRKQFPILDQKVNGKQLVYFDSAATSQKPIQVIETLERYYKEYNSNVHRGVHTLGTKATD 65 Query: 66 AYEGAREKLAKFINARSTKEIIFTRGTTTALNIVASSYGPGNVGEGDEIVITQMEHHSNF 125 AYEGAREK+ KFINA+S +EIIFTRGTTTALN VA+SYG NV EGDEIVI+ MEHHSN Sbjct: 66 AYEGAREKVRKFINAKSMEEIIFTRGTTTALNTVAASYGLENVKEGDEIVISYMEHHSNI 125 Query: 126 IPWQQLAKKTGATLKFIPLQKDGSITLEDAEQTITDKTKIVAIAYVSNVMGISHPVKELA 185 IPWQQ+AKKTGATLK++PLQ DG+I++EDA QTIT TKIV+I YVSNV+G +PVKE+ Sbjct: 126 IPWQQVAKKTGATLKYLPLQPDGTISIEDARQTITPNTKIVSIMYVSNVLGTINPVKEIG 185 Query: 186 AIAHRHGAVIVVDGAQSTPHMKVDVQDLDCDFYALSGHKMLAPTGIGALYGKKALLEAME 245 AIAH +GA++VVDGAQSTPHMKVDVQDL+CDFYALS HKM PTGIG LYGKK LL ME Sbjct: 186 AIAHENGAIMVVDGAQSTPHMKVDVQDLNCDFYALSAHKMCGPTGIGVLYGKKELLNNME 245 Query: 246 PVEFGGEMIDDVGLYDSTWKELPWKFEGGTPXXXXXXXXXXXXDFLQEIGLDEIHRHEME 305 P+EFGGEMID V L +STWKELPWKFE GTP DFL+EIGLD I +HE E Sbjct: 246 PIEFGGEMIDFVDLQESTWKELPWKFEAGTPIIGNAIGLGAAIDFLEEIGLDNIEKHEHE 305 Query: 306 LAAYAEQRLSEVDGLTIYGPRNRKVGVVTFNLGDVHPHDVATVLDAEGVAVRAGHHCCQP 365 LA YA +RLSEVDG+TIYGP+NR G+VTFN+ DVHPHDVATVLD EG+AVRAGHHC QP Sbjct: 306 LAQYALERLSEVDGVTIYGPKNR-AGLVTFNIEDVHPHDVATVLDVEGIAVRAGHHCAQP 364 Query: 366 LMRWLQVSSTARASFYLYNSEQDVDALVQALIKAKEYF 403 LM+WL+ SSTARASFYLYN+++++D V +LIK KEYF Sbjct: 365 LMKWLKASSTARASFYLYNTKEEIDTFVDSLIKTKEYF 402 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,298,699,165 Number of extensions: 91708937 Number of successful extensions: 254567 Number of sequences better than 1.0e-05: 5028 Number of HSP's gapped: 247136 Number of HSP's successfully gapped: 5036 Length of query: 408 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 270 Effective length of database: 1,760,596,133 Effective search space: 475360955910 Effective search space used: 475360955910 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)