BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0028.p (270 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DE22.1 B1DE22_9BACL Phosphoesterase 341 4e-92 C1QQC5.1 C1QQC5_9ACTO Phosphatidylinositol-3-phosphate phosphata... 284 7e-75 Q0RKU3.1 Q0RKU3_FRAAA Putative acid phosphatase EC=3.1.3.2 265 4e-69 Q7TWR9.1 Q7TWR9_MYCBO POSSIBLE ACID PHOSPHATASE (ACID PHOSPHOMON... 260 1e-67 C1AH84.1 C1AH84_MYCBT Putative acid phosphatase EC=3.1.3.2 260 1e-67 >B1DE22.1 B1DE22_9BACL Phosphoesterase Length = 290 Score = 341 bits (875), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 158/263 (60%), Positives = 200/263 (76%), Gaps = 5/263 (1%) Query: 7 VHPGSATAAQPAPASKSAYDHIVIVVEENHSSRKILNNSSAPFMNSLAKQGLSLTNHYAI 66 VH S T ++ A +HIVIVVEENHS ++I+ N +AP++N L K G +LTNH+AI Sbjct: 31 VHASSTTTQ-----TRLAPNHIVIVVEENHSYKEIIGNKNAPYINQLVKNGANLTNHFAI 85 Query: 67 THPSQPNYIELFSGSNQGVTDDLSHPRTNHANLASELISHGYSFGGYSEGLPKVGYTGPY 126 HPSQPNYI+LFSGSNQGVTDD NLA+ELI HGY+FGGYSEGLP G+TGPY Sbjct: 86 EHPSQPNYIDLFSGSNQGVTDDKPSHTFKTDNLANELIKHGYTFGGYSEGLPSAGFTGPY 145 Query: 127 DLSTKYARKHNPWVNFSNLPSSINMPLTSFPKDFNQLPTVSFVIPNLNNDMHDGSIKTAD 186 DL T YARKHNPWV+F+N+ + +NMP SFPKD+++LPTVSFV+PNL +D+HDG+IK AD Sbjct: 146 DLKTLYARKHNPWVDFTNVSAKVNMPFISFPKDYSKLPTVSFVVPNLKHDIHDGTIKEAD 205 Query: 187 QWLKSHLSAYAVWAANHNSLLIVTWDEDDMSEHNKIPTFIVGAKVKKGSYTVKTNHYSLL 246 WLK++++ Y WA HNS+ I+TWDEDD S NKIPT ++G K+K +Y KTNHY++L Sbjct: 206 DWLKNNMAGYIEWAKKHNSIFILTWDEDDRSAKNKIPTIMIGQKIKPQAYITKTNHYNML 265 Query: 247 RTLEDMYGLNTLGKSKAAQPIKI 269 RT+ED YGL LG SK +PI + Sbjct: 266 RTIEDFYGLPALGNSKNIKPINV 288 >C1QQC5.1 C1QQC5_9ACTO Phosphatidylinositol-3-phosphate phosphatase EC=3.1.3.- Length = 292 Score = 284 bits (726), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 140/252 (55%), Positives = 179/252 (71%), Gaps = 9/252 (3%) Query: 17 PAPASKSAYDHIVIVVEENHSSRKILNNSSAPFMNSLAKQGLSLTNHYAITHPSQPNYIE 76 P PA H+V+V+EENHS I+ +SSAP++NSLA G LT +A+THPS+PNY+ Sbjct: 44 PTPA------HVVVVMEENHSYNDIIGSSSAPYINSLASSGALLTGSFAVTHPSEPNYMA 97 Query: 77 LFSGSNQGVTDDLSH-PRTNHANLASELISHGYSFGGYSEGLPKVGYTGPYDLSTKYARK 135 LF GS G++ D N ANL SEL+ G +F GYSEGLP G T S KYARK Sbjct: 98 LFGGSTFGLSSDACPVSEGNTANLGSELLGAGKTFKGYSEGLPSTGSTTCS--SGKYARK 155 Query: 136 HNPWVNFSNLPSSINMPLTSFPKDFNQLPTVSFVIPNLNNDMHDGSIKTADQWLKSHLSA 195 H+PWVN+SN+P S ++P + FP +++ LPT+SFVIPNLN+DMHDG++KT D WLK++LSA Sbjct: 156 HSPWVNYSNVPGSDSLPFSQFPTNYSTLPTLSFVIPNLNDDMHDGTVKTGDTWLKTNLSA 215 Query: 196 YAVWAANHNSLLIVTWDEDDMSEHNKIPTFIVGAKVKKGSYTVKTNHYSLLRTLEDMYGL 255 YA WA +NS+LIVTWDEDD +E N+IPT +VGA VK G Y+ + HY+LL TLE MYGL Sbjct: 216 YATWAKANNSVLIVTWDEDDYTESNQIPTIVVGAGVKTGHYSEQVTHYNLLATLEKMYGL 275 Query: 256 NTLGKSKAAQPI 267 +G S A I Sbjct: 276 TPVGNSSGAAVI 287 >Q0RKU3.1 Q0RKU3_FRAAA Putative acid phosphatase EC=3.1.3.2 Length = 294 Score = 265 bits (676), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 132/246 (53%), Positives = 171/246 (69%), Gaps = 4/246 (1%) Query: 26 DHIVIVVEENHSSRKILNNSSAPFMNSLAKQGLSLTNHYAITHPSQPNYIELFSGSNQGV 85 DH+V+V+ ENHS +++ +SSAP++NSL G +LT YA+THPSQPNY+ LFSG QG+ Sbjct: 44 DHVVVVMLENHSYSQVIGSSSAPYINSLKSGGANLTASYALTHPSQPNYVALFSGGTQGI 103 Query: 86 TDDLSHP--RTNHANLASELISHGYSFGGYSEGLPKVGYTGPYDLSTKYARKHNPWVNFS 143 TDD + ++ ANL SELI+ G +FG Y+EGLP G T + +TKYARKHNPW FS Sbjct: 104 TDDACYTPGFSSAANLGSELIAAGKTFGSYNEGLPSAGSTVCTNSATKYARKHNPWFAFS 163 Query: 144 NLPSSINMPLTSFPKDFNQLPTVSFVIPNLNNDMHDGSIKTADQWLKSHLSAYAVWAANH 203 N+P+S FP +++ LP VSFVIPNL NDMHD S+ T D WL+++L AYA WA H Sbjct: 164 NVPTSTAHTFAQFPTNYSTLPKVSFVIPNLCNDMHDCSVATGDTWLQNNLGAYATWAKTH 223 Query: 204 NSLLIVTWDEDDMSEHNKIPTFIVGAKVKKGSYTVKT-NHYSLLRTLEDMYGLNT-LGKS 261 NS+L+VT+DED+ N+IPT G V GS T T NHY+LLRTLED+ GL T G + Sbjct: 224 NSVLVVTFDEDNSQSGNRIPTVFYGQHVTAGSSTSTTYNHYNLLRTLEDLGGLTTHAGNA 283 Query: 262 KAAQPI 267 +A I Sbjct: 284 SSASDI 289 >Q7TWR9.1 Q7TWR9_MYCBO POSSIBLE ACID PHOSPHATASE (ACID PHOSPHOMONOESTERASE) (PHOSPHOMONOESTERASE) (GLYCEROPHOSPHATASE) EC=3.1.3.2 Length = 299 Score = 260 bits (664), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 132/247 (53%), Positives = 171/247 (69%), Gaps = 7/247 (2%) Query: 25 YDHIVIVVEENHSSRKILNNSSAPFMNSLAKQGLSLTNHYAITHPSQPNYIELFSGSNQG 84 + H+VIVVEEN S I+ N SAPF+NSLA G + +A THPS+PNY+ LF+G+ G Sbjct: 50 FAHVVIVVEENRSQAAIIGNKSAPFINSLAANGAMMAQAFAETHPSEPNYLALFAGNTFG 109 Query: 85 VTDDLSHPRTNHA--NLASELISHGYSFGGYSEGLPKVGYTGPYDLSTKYARKHNPWVNF 142 +T + + P A NL SEL+S GY+F G++E LP VG T + KYARKH PWVNF Sbjct: 110 LTKN-TCPVNGGALPNLGSELLSAGYTFMGFAEDLPAVGST--VCSAGKYARKHVPWVNF 166 Query: 143 SNLPSSINMPLTSFPK--DFNQLPTVSFVIPNLNNDMHDGSIKTADQWLKSHLSAYAVWA 200 SN+P+++++P ++FPK ++ LPTVSFVIPN +NDMHDGSI D WL HLSAYA WA Sbjct: 167 SNVPATLSVPFSAFPKPQNYPGLPTVSFVIPNADNDMHDGSIAQGDAWLNRHLSAYANWA 226 Query: 201 ANHNSLLIVTWDEDDMSEHNKIPTFIVGAKVKKGSYTVKTNHYSLLRTLEDMYGLNTLGK 260 +NSLL+VTWDEDD S N+IPT GA V+ G+Y +HY++L TLE +YGL G Sbjct: 227 KTNNSLLVVTWDEDDGSSRNQIPTVFYGAHVRPGTYNETISHYNVLSTLEQIYGLPKTGY 286 Query: 261 SKAAQPI 267 + A PI Sbjct: 287 ATNAPPI 293 >C1AH84.1 C1AH84_MYCBT Putative acid phosphatase EC=3.1.3.2 Length = 299 Score = 260 bits (664), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 132/247 (53%), Positives = 171/247 (69%), Gaps = 7/247 (2%) Query: 25 YDHIVIVVEENHSSRKILNNSSAPFMNSLAKQGLSLTNHYAITHPSQPNYIELFSGSNQG 84 + H+VIVVEEN S I+ N SAPF+NSLA G + +A THPS+PNY+ LF+G+ G Sbjct: 50 FAHVVIVVEENRSQAAIIGNKSAPFINSLAANGAMMAQAFAETHPSEPNYLALFAGNTFG 109 Query: 85 VTDDLSHPRTNHA--NLASELISHGYSFGGYSEGLPKVGYTGPYDLSTKYARKHNPWVNF 142 +T + + P A NL SEL+S GY+F G++E LP VG T + KYARKH PWVNF Sbjct: 110 LTKN-TCPVNGGALPNLGSELLSAGYTFMGFAEDLPAVGST--VCSAGKYARKHVPWVNF 166 Query: 143 SNLPSSINMPLTSFPK--DFNQLPTVSFVIPNLNNDMHDGSIKTADQWLKSHLSAYAVWA 200 SN+P+++++P ++FPK ++ LPTVSFVIPN +NDMHDGSI D WL HLSAYA WA Sbjct: 167 SNVPATLSVPFSAFPKPQNYPGLPTVSFVIPNADNDMHDGSIAQGDAWLNRHLSAYANWA 226 Query: 201 ANHNSLLIVTWDEDDMSEHNKIPTFIVGAKVKKGSYTVKTNHYSLLRTLEDMYGLNTLGK 260 +NSLL+VTWDEDD S N+IPT GA V+ G+Y +HY++L TLE +YGL G Sbjct: 227 KTNNSLLVVTWDEDDGSSRNQIPTVFYGAHVRPGTYNETISHYNVLSTLEQIYGLPKTGY 286 Query: 261 SKAAQPI 267 + A PI Sbjct: 287 ATNAPPI 293 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.128 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,688,197,845 Number of extensions: 70611087 Number of successful extensions: 129813 Number of sequences better than 1.0e-05: 104 Number of HSP's gapped: 129500 Number of HSP's successfully gapped: 104 Length of query: 270 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 137 Effective length of database: 1,807,701,208 Effective search space: 247655065496 Effective search space used: 247655065496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)