BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0012.p (346 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBK2.1 B3KBK2_9BACI Metal dependent phophohydrolase 428 e-118 B1DKG5.1 B1DKG5_9BACL Metal dependent phosphohydrolase 344 7e-93 A3DDV3.1 A3DDV3_CLOTH Metal dependent phosphohydrolase 205 5e-51 B4BFA5.1 B4BFA5_CLOTM Metal dependent phosphohydrolase 205 5e-51 B2A7I2.1 B2A7I2_NATTJ Metal dependent phosphohydrolase 197 1e-48 >B3KBK2.1 B3KBK2_9BACI Metal dependent phophohydrolase Length = 348 Score = 428 bits (1100), Expect = e-118, Method: Compositional matrix adjust. Identities = 203/348 (58%), Positives = 268/348 (77%), Gaps = 3/348 (0%) Query: 1 LRVHVTDLKPGDYLKVDTFSSKGLHVLPKGSQLRIEEIAKLVQHGVDYVDIE--AIQEEP 58 +RVHVTDLKPGD ++ D +++ G+HVL KG+ LR E+I+KL+QHG+DY++I+ A+ Sbjct: 1 MRVHVTDLKPGDQIQQDAYNTNGVHVLQKGALLRSEDISKLLQHGIDYIEIQPRALSVSL 60 Query: 59 APXXXXXXXXXXXR-NFDTMIDGFESVYMEALTKGSFNQSVVDDILQPSLESLDKHKDVV 117 P + +F+ DG +++ EA G FN+SVVDD+ QP + SL++H DVV Sbjct: 61 DPLSEISDSFNKVKPHFNLAFDGCSALFTEASQSGKFNESVVDDVFQPLVGSLNEHTDVV 120 Query: 118 SLLLLLDREDNYTYNHSLQVGMLSYYIATWLGYSKKECYEIGRAGYLIDIGKCRISPELL 177 SLLLL + D+YTY HS+QVGMLSYYIA W+GYS KE YEIG+AGYL DIGK +IS ++L Sbjct: 121 SLLLLFNGTDDYTYRHSIQVGMLSYYIADWMGYSGKEAYEIGKAGYLHDIGKSKISRDIL 180 Query: 178 NKPGKLTAAEYDEVKLHTIYGYEIIRNSMDDPYTALVALQHHEREDGSGYPHNLTKDDIH 237 +KPGKLT E++EVKLHTIYGYE+I SMDD TA+VALQHHER+D SGYP L D IH Sbjct: 181 DKPGKLTPEEFEEVKLHTIYGYEMIAESMDDKVTAMVALQHHERDDRSGYPRALHADQIH 240 Query: 238 PYSQIAAVADIYSAMTSERIYQSKQELISVLREINTLSFGKLNGKPVQAFIQHLMPNFIG 297 PYS I AVAD+YSAMT+ R+YQSKQ+L++VLRE+ +LSFGKLNGKP AFIQ ++PNFIG Sbjct: 241 PYSHICAVADVYSAMTTNRVYQSKQQLLTVLRELYSLSFGKLNGKPTHAFIQQMLPNFIG 300 Query: 298 KRVLLSTGDMGVIIMNNPLDVFRPLVQIEGKFLDLARERTIAVAEIYM 345 K+VLL+TG+ G+I+MN+P D FRPLV+ + +F+DL++E + EI + Sbjct: 301 KKVLLTTGESGIIVMNHPSDYFRPLVKTDDRFIDLSKEYHTEIQEILL 348 >B1DKG5.1 B1DKG5_9BACL Metal dependent phosphohydrolase Length = 354 Score = 344 bits (882), Expect = 7e-93, Method: Compositional matrix adjust. Identities = 168/352 (47%), Positives = 235/352 (66%), Gaps = 9/352 (2%) Query: 1 LRVHVTDLKPGDYLKVDTFSSKGLHVLPKGSQLRIEEIAKLVQHGVDYVDIEAIQEEPAP 60 +R+H++DLK GD L DTF+ GLHVL KG+ L ++IAKL QH VDYVDIE Q P Sbjct: 1 MRLHISDLKEGDILLKDTFNGYGLHVLSKGTVLAQKDIAKLFQHQVDYVDIEDRQTTEQP 60 Query: 61 XXXXXXXXXXXR-------NFDTMIDGFESVYMEALTKGSFNQSVVDDILQPSLESLDKH 113 + +++ + GF+ ++ +A G Q V QP L++ Sbjct: 61 IHRTLETTVNPKWLPNVRPDYENAVKGFQGLFQKATDGGVVKQDEVAAAFQPLLKNFQME 120 Query: 114 KDVVSLLLLLDREDNYTYNHSLQVGMLSYYIATWLGYSKKECYEIGRAGYLIDIGKCRIS 173 +DVVS+LLLL+ D+YTY HS+QVGMLSYY+A+WLGY++ E EI ++GYL DIGK RI Sbjct: 121 RDVVSMLLLLNTADDYTYQHSVQVGMLSYYLASWLGYTEMEALEISKSGYLHDIGKSRIP 180 Query: 174 PELLNKPGKLTAAEYDEVKLHTIYGYEIIRNSMDDP--YTALVALQHHEREDGSGYPHNL 231 +LNKP KLT E++EVK HT+YGY+II+ S +D A+ ALQHHER DGSGYPH L Sbjct: 181 SSILNKPAKLTDEEFEEVKKHTLYGYDIIQQSFEDKSEIMAVSALQHHERNDGSGYPHGL 240 Query: 232 TKDDIHPYSQIAAVADIYSAMTSERIYQSKQELISVLREINTLSFGKLNGKPVQAFIQHL 291 D+IHP +++ AV DIYSAM SER+Y+ K++L VL+E+ +SF +L+ FI+H+ Sbjct: 241 RGDEIHPVAKLIAVVDIYSAMISERVYKKKRDLFFVLKELYKMSFNELDAHTTHTFIKHM 300 Query: 292 MPNFIGKRVLLSTGDMGVIIMNNPLDVFRPLVQIEGKFLDLARERTIAVAEI 343 +PNFIGK+ +L +G++G I+M +P + FRPL+Q +F+DL +R + EI Sbjct: 301 IPNFIGKKAVLESGEVGTIVMTHPTEFFRPLIQTGERFIDLTVDREYEIKEI 352 >A3DDV3.1 A3DDV3_CLOTH Metal dependent phosphohydrolase Length = 345 Score = 205 bits (522), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 114/338 (33%), Positives = 186/338 (55%), Gaps = 4/338 (1%) Query: 7 DLKPGDYLKVDTFSSKGLHVLPKGSQLRIEEIAKLVQHGVDYVDIEAIQEEPAPXXXXXX 66 ++KPG + + G V+ +G+ L I KLV ++Y + I E+ Sbjct: 10 EVKPGMRIAETIMNDYGSVVISEGTILDDHMIKKLV--NLNYTKVRVI-EKDKNFVSSEI 66 Query: 67 XXXXXRNFDTMIDGFESVYMEALTKGSFNQSVVDDILQPSLESLDKHKDVVSLLLLLDRE 126 +++ ++ F+S+ +A + N VD ++ + LD+ +D+V + + Sbjct: 67 SESFNVSYNASVNCFKSILYDAAVGKNINMEKVDKVVMSVVARLDEKRDIVRCINQIKSA 126 Query: 127 DNYTYNHSLQVGMLSYYIATWLGYSKKECYEIGRAGYLIDIGKCRISPELLNKPGKLTAA 186 D YTY H + V +L + WLGYSK ++ +AG L DIGK ++ E+LNKP KLT Sbjct: 127 DQYTYTHCVNVSLLCMLLGKWLGYSKDSLIKLVQAGLLHDIGKAKVPKEILNKPSKLTPE 186 Query: 187 EYDEVKLHTIYGYEIIRNSMD-DPYTALVALQHHEREDGSGYPHNLTKDDIHPYSQIAAV 245 EY+E+K HT+YGY I+ + D A+ L HHER DGSGYP D I+ +++ AV Sbjct: 187 EYEEIKKHTVYGYRILEKEHEIDRDVAIAVLMHHERMDGSGYPTGAKSDQINMMARVIAV 246 Query: 246 ADIYSAMTSERIYQSKQELISVLREINTLSFGKLNGKPVQAFIQHLMPNFIGKRVLLSTG 305 ADIY AMTS R+Y+ K+ V I + GKL+ + V AF+ ++ +IG L+TG Sbjct: 247 ADIYDAMTSNRVYREKESPFEVFHIIEEEAVGKLDVRVVNAFLSNIAAYYIGDFAKLNTG 306 Query: 306 DMGVIIMNNPLDVFRPLVQIEGKFLDLARERTIAVAEI 343 ++ I+ NP V RP+++++ +++DL E+ + + EI Sbjct: 307 EICEIVYINPRYVSRPIIRVDDQYIDLTVEKDLKILEI 344 >B4BFA5.1 B4BFA5_CLOTM Metal dependent phosphohydrolase Length = 345 Score = 205 bits (522), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 114/338 (33%), Positives = 186/338 (55%), Gaps = 4/338 (1%) Query: 7 DLKPGDYLKVDTFSSKGLHVLPKGSQLRIEEIAKLVQHGVDYVDIEAIQEEPAPXXXXXX 66 ++KPG + + G V+ +G+ L I KLV ++Y + I E+ Sbjct: 10 EVKPGMRIAETIMNDYGSVVISEGTILDDHMIKKLV--NLNYTKVRVI-EKDKNFVSSEI 66 Query: 67 XXXXXRNFDTMIDGFESVYMEALTKGSFNQSVVDDILQPSLESLDKHKDVVSLLLLLDRE 126 +++ ++ F+S+ +A + N VD ++ + LD+ +D+V + + Sbjct: 67 SESFNVSYNASVNCFKSILYDAAVGKNINMEKVDKVVMSVVARLDEKRDIVRCINQIKSA 126 Query: 127 DNYTYNHSLQVGMLSYYIATWLGYSKKECYEIGRAGYLIDIGKCRISPELLNKPGKLTAA 186 D YTY H + V +L + WLGYSK ++ +AG L DIGK ++ E+LNKP KLT Sbjct: 127 DQYTYTHCVNVSLLCMLLGKWLGYSKDSLIKLVQAGLLHDIGKAKVPKEILNKPSKLTPE 186 Query: 187 EYDEVKLHTIYGYEIIRNSMD-DPYTALVALQHHEREDGSGYPHNLTKDDIHPYSQIAAV 245 EY+E+K HT+YGY I+ + D A+ L HHER DGSGYP D I+ +++ AV Sbjct: 187 EYEEIKKHTVYGYRILEKEHEIDRDVAIAVLMHHERMDGSGYPTGAKSDQINMMARVIAV 246 Query: 246 ADIYSAMTSERIYQSKQELISVLREINTLSFGKLNGKPVQAFIQHLMPNFIGKRVLLSTG 305 ADIY AMTS R+Y+ K+ V I + GKL+ + V AF+ ++ +IG L+TG Sbjct: 247 ADIYDAMTSNRVYREKESPFEVFHIIEEEAVGKLDVRVVNAFLSNIAAYYIGDFAKLNTG 306 Query: 306 DMGVIIMNNPLDVFRPLVQIEGKFLDLARERTIAVAEI 343 ++ I+ NP V RP+++++ +++DL E+ + + EI Sbjct: 307 EICEIVYINPRYVSRPIIRVDDQYIDLTVEKDLKILEI 344 >B2A7I2.1 B2A7I2_NATTJ Metal dependent phosphohydrolase Length = 346 Score = 197 bits (501), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 124/347 (35%), Positives = 185/347 (53%), Gaps = 11/347 (3%) Query: 2 RVHVTDLKPGDYLKVDTFSSKGLHVLPKGSQLRIEEIAKLVQHGVDYVDIEAIQEEPAPX 61 R V LK G L D S G ++PKG L + I L+ G+ V IE +E+ Sbjct: 3 RYSVFQLKEGMKLSKDVNSKSGATLVPKGVILSKKTIKFLINWGIKSVLIEEEEEQITRG 62 Query: 62 XXXXXXXXXXRNFDTMIDGFESV--YMEALTKGSFNQSVVDDILQPSLESLDKHKDVVSL 119 F+T E V +ME L KGS +D++ + + E + D+ S+ Sbjct: 63 RGHSQAKFKKEYFETT----EKVISFMEGL-KGS-KAIELDEVKEIAQELVTYSDDINSV 116 Query: 120 LLLLDREDN--YTYNHSLQVGMLSYYIATWLGYSKKECYEIGRAGYLIDIGKCRISPELL 177 LL + +D YTY HS+ VG+ + + WLGY + + AG L DIGK +I E+L Sbjct: 117 KLLTEVKDKDYYTYQHSINVGIYASLLGRWLGYCTNDIKNLSLAGLLHDIGKTQIPDEVL 176 Query: 178 NKPGKLTAAEYDEVKLHTIYGYEIIRNSMD-DPYTALVALQHHEREDGSGYPHNLTKDDI 236 KP LT E++ +K H IYGYE+++ D P + LQHHERE+GSGYP +T + I Sbjct: 177 LKPESLTDKEFEIIKNHPIYGYELLKPCEDLSPEINIAVLQHHERENGSGYPFGITGEKI 236 Query: 237 HPYSQIAAVADIYSAMTSERIYQSKQELISVLREINTLSFGKLNGKPVQAFIQHLMPNFI 296 H YS+I +V DI+ A+TS R+Y+ + +RE+ SF LN + F + + F+ Sbjct: 237 HSYSKIISVVDIFDAITSNRVYKGRNSPFEAIREVRDKSFQDLNPEIAYTFFKRISEFFV 296 Query: 297 GKRVLLSTGDMGVIIMNNPLDVFRPLVQIEGKFLDLARERTIAVAEI 343 G V+ + G +G I+ N D RPLVQIE KF+DL++ + + ++ Sbjct: 297 GSTVIFNDGSIGKIVFLNKNDPTRPLVQIEDKFVDLSKHKNREIIDV 343 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,993,616,046 Number of extensions: 80796634 Number of successful extensions: 200544 Number of sequences better than 1.0e-05: 2305 Number of HSP's gapped: 198619 Number of HSP's successfully gapped: 2305 Length of query: 346 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 210 Effective length of database: 1,779,438,163 Effective search space: 373682014230 Effective search space used: 373682014230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)