BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0010.p (213 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBK4.1 B3KBK4_9BACI 5'-Nucleotidase domain protein 249 8e-65 B1DKG2.1 B1DKG2_9BACL Metallophosphoesterase 165 3e-39 C1P856.1 C1P856_BACCO Metallophosphoesterase 148 3e-34 B4AG33.1 B4AG33_BACPU Bifunctional 5'-nucleotidase/2',3'-cyclic ... 147 8e-34 A8FH39.1 A8FH39_BACP2 Bifunctional 5'-nucleotidase/2',3'-cyclic ... 145 2e-33 >B3KBK4.1 B3KBK4_9BACI 5'-Nucleotidase domain protein Length = 477 Score = 249 bits (637), Expect = 8e-65, Method: Compositional matrix adjust. Identities = 117/178 (65%), Positives = 143/178 (80%), Gaps = 1/178 (0%) Query: 6 QRLTLIHTNDIHSHFEMMSPIXXXXXXXXXXXGEEPVLLLDIGDHMDRAAVETEGTMGQA 65 Q+LT+++TNDIHSHFEMMS + G++ ++L DIGDHMDRA+VETEG+MGQA Sbjct: 8 QKLTILYTNDIHSHFEMMSNVAALISREKTAAGDKSIVL-DIGDHMDRASVETEGSMGQA 66 Query: 66 NIDMINLTGYDAITIGNNEGLTFTREILSALYAGLQCPVVCCNILESATGEAPGWMKRHI 125 N+D+INLTGYDAITIGNNEGLT + ++L +YAGLQCPVVCCNI E+ +G P WMKRH+ Sbjct: 67 NVDVINLTGYDAITIGNNEGLTISYDVLEHVYAGLQCPVVCCNIRETESGSPPSWMKRHV 126 Query: 126 ILEKDGIKIGITGATAAFASFYGLLGWEALDPEEALREQCELLAPQVDLLIILSHLGL 183 ILE+DGI IG+TGATAAFA FY LLGWEA DP EA+ EQ L Q D++I+LSHLGL Sbjct: 127 ILERDGISIGLTGATAAFAGFYQLLGWEAGDPVEAIAEQVRALQGQCDIVIVLSHLGL 184 >B1DKG2.1 B1DKG2_9BACL Metallophosphoesterase Length = 479 Score = 165 bits (417), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 75/178 (42%), Positives = 116/178 (65%) Query: 6 QRLTLIHTNDIHSHFEMMSPIXXXXXXXXXXXGEEPVLLLDIGDHMDRAAVETEGTMGQA 65 + + ++H+NDIHS E + I G E +L LDIGDHMDR +ETEG+ G Sbjct: 6 RSILILHSNDIHSRLENAARIMSYIEEQRAAYGSERILCLDIGDHMDRIRLETEGSDGMV 65 Query: 66 NIDMINLTGYDAITIGNNEGLTFTREILSALYAGLQCPVVCCNILESATGEAPGWMKRHI 125 N++++N GYDA+T+GNNEGLT++++ L +Y + ++ N+ E +G+ PGW+ Sbjct: 66 NVELLNAAGYDAVTLGNNEGLTYSKDTLERVYREAKFTILGANMRELESGKLPGWLLPST 125 Query: 126 ILEKDGIKIGITGATAAFASFYGLLGWEALDPEEALREQCELLAPQVDLLIILSHLGL 183 ++EK+GI+IG+ ATA + FY LLGW+ DP EA+ E+ L QVD++ ++SHLG+ Sbjct: 126 VMEKNGIRIGLVAATARYTDFYELLGWDLTDPFEAIGEEVRRLRDQVDVIAVMSHLGI 183 >C1P856.1 C1P856_BACCO Metallophosphoesterase Length = 472 Score = 148 bits (374), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 80/179 (44%), Positives = 105/179 (58%), Gaps = 4/179 (2%) Query: 8 LTLIHTNDIHSHFEMMSPIXXXXXXXXXXXGE--EPVLLLDIGDHMDRAAVETEGTMGQA 65 L + HTNDIHSHFE I E +LL D+GDH+DR TEGTMG+A Sbjct: 21 LHIYHTNDIHSHFENWPKIRHFLERKQAQHARQNEEMLLFDLGDHVDRWHPYTEGTMGKA 80 Query: 66 NIDMINLTGYDAITIGNNEGLTFTREILSALYAGLQCPVVCCNILESATGEAPGW-MKRH 124 N+ ++N GY A TIGNNEG+T E L LY Q V+ N+ + G+ P W + R Sbjct: 81 NVRLLNQLGYLAATIGNNEGITMPHEALDTLYEEAQFDVILANLFDE-NGKRPQWAVPRK 139 Query: 125 IILEKDGIKIGITGATAAFASFYGLLGWEALDPEEALREQCELLAPQVDLLIILSHLGL 183 + K G+KIG+TG TA F Y LLGW+ +P E L +Q +L + D+LI+LSHLG+ Sbjct: 140 LYTTKSGVKIGVTGVTAGFYKLYRLLGWQLSNPLEELGKQMNILKKEADILIVLSHLGI 198 >B4AG33.1 B4AG33_BACPU Bifunctional 5'-nucleotidase/2',3'-cyclic phosphodiesterase Length = 457 Score = 147 bits (370), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 75/180 (41%), Positives = 107/180 (59%), Gaps = 5/180 (2%) Query: 5 LQRLTLIHTNDIHSHFEMMSPIXXXXXXXXXXXGEEPVLLLDIGDHMDRAAVETEGTMGQ 64 LQ+L L HTND+HSHFE I + +LL DIGDHMDR + +E G+ Sbjct: 2 LQKLWLYHTNDLHSHFENWPKIAAYIEEKRQV--HDQMLLFDIGDHMDRVNLVSEADYGK 59 Query: 65 ANIDMINLTGYDAITIGNNEGLTFTREILSALYAGLQCPVVCCNILESATGEAPGWMK-R 123 N+ ++N GYD +TIGNNEG+T + L LY Q PV+ N+ E G+ P W K Sbjct: 60 VNVKLLNQLGYDGVTIGNNEGITLPHDQLDQLYEHAQFPVILSNLFEK--GKRPAWAKPY 117 Query: 124 HIILEKDGIKIGITGATAAFASFYGLLGWEALDPEEALREQCELLAPQVDLLIILSHLGL 183 HII ++G+KI + G T A+ Y LGWE DP +++R+ + + Q D++++LSHLG+ Sbjct: 118 HIIQMENGLKICLLGVTIAYTPVYEKLGWEITDPFDSIRDMLQEVKGQADIIVLLSHLGI 177 >A8FH39.1 A8FH39_BACP2 Bifunctional 5'-nucleotidase/2',3'-cyclic phosphodiesterase Length = 457 Score = 145 bits (366), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 74/180 (41%), Positives = 107/180 (59%), Gaps = 5/180 (2%) Query: 5 LQRLTLIHTNDIHSHFEMMSPIXXXXXXXXXXXGEEPVLLLDIGDHMDRAAVETEGTMGQ 64 LQ+L + HTND+HSHFE I + +LL DIGDHMDR + +E G+ Sbjct: 2 LQKLWIYHTNDLHSHFENWPKIAAYIKEKRQI--HDQMLLFDIGDHMDRVNLVSEADYGK 59 Query: 65 ANIDMINLTGYDAITIGNNEGLTFTREILSALYAGLQCPVVCCNILESATGEAPGWMK-R 123 N+ ++N GYD +TIGNNEG+T + L LY Q PVV N+ E G+ P W K Sbjct: 60 VNVKLLNQLGYDGVTIGNNEGITLPHDQLDQLYEHAQFPVVLSNLFEK--GKRPSWAKPY 117 Query: 124 HIILEKDGIKIGITGATAAFASFYGLLGWEALDPEEALREQCELLAPQVDLLIILSHLGL 183 H+I ++G+KI + G T A+ Y LGWE DP +++R+ + + Q D++++LSHLG+ Sbjct: 118 HMIQMENGLKICLLGVTIAYTPVYEKLGWEITDPFDSIRDMLQEVKGQADVIVLLSHLGI 177 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.139 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,183,092,305 Number of extensions: 44828090 Number of successful extensions: 88761 Number of sequences better than 1.0e-05: 421 Number of HSP's gapped: 88471 Number of HSP's successfully gapped: 421 Length of query: 213 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 83 Effective length of database: 1,835,964,253 Effective search space: 152385032999 Effective search space used: 152385032999 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 128 (53.9 bits)