BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0009.p (275 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBK4.1 B3KBK4_9BACI 5'-Nucleotidase domain protein 297 7e-79 B1DKG2.1 B1DKG2_9BACL Metallophosphoesterase 191 8e-47 C0ZKY6.1 C0ZKY6_BREBN Putative nucleotidase EC=3.1.3.- 145 5e-33 Q5KVL8.1 Q5KVL8_GEOKA Hypothetical conserved protein 145 6e-33 Q815Z7.1 Q815Z7_BACCR 5'-nucleotidase EC=3.1.3.5 142 2e-32 >B3KBK4.1 B3KBK4_9BACI 5'-Nucleotidase domain protein Length = 477 Score = 297 bits (761), Expect = 7e-79, Method: Compositional matrix adjust. Identities = 150/270 (55%), Positives = 186/270 (68%), Gaps = 2/270 (0%) Query: 1 MLETPQMINGTAVCGAGKFGRYIGRLIFERAHAGAAFELVSGRCFTVDPSLTEDVLAPAA 60 +LE P MI TAVCGAGKFG+Y+GRL+ ER G F V G VD SL E ++ A Sbjct: 208 ILEEPLMIGKTAVCGAGKFGQYVGRLVMERNDVGERFTCVEGTLLPVDKSLMEPIVEQAI 267 Query: 61 APHLQHGREVLNETVAITDRELTLDTEGESPFGNLLAQAVRRFTGAPISLVNSGQLLGPL 120 H + +E L E VA+ DR L + E ESPF NLLAQAV + TGA I++VNSGQLLGPL Sbjct: 268 LKHHELAKEKLTEAVAVIDRSLPIHYEEESPFANLLAQAVLQHTGAEIAIVNSGQLLGPL 327 Query: 121 PQGTITAGMLHALCPSPVNACIIRLKGEDIRKALEQSRMEAFYNKAIFGYGFRGKVLGSL 180 P+G I+ GMLH LCPSP+N C+++L+GE I +ALE+S + F K I G+GFRG +LG L Sbjct: 328 PEGEISTGMLHGLCPSPINPCVVKLRGEHIYQALEESLLPEFSGKKIMGFGFRGYLLGGL 387 Query: 181 AVDGLKILYDPGVLPYDNGNGIAVFVGGEPLDDKAVYSVGTLDMFTFRIGYESLADGTEP 240 AVDGLKI YD PY I+ G+PL+ + Y VGTLDMFTF+IGYE+LA GT+ Sbjct: 388 AVDGLKIRYDKDGEPYRKITQIS--FKGQPLEWQREYHVGTLDMFTFKIGYETLALGTDT 445 Query: 241 LYLLPHFLRDLLRMELQRPGSLDECAGLRW 270 +Y+LP F+RDLLR EL RPGSL+ RW Sbjct: 446 VYMLPEFIRDLLRSELSRPGSLEASMISRW 475 >B1DKG2.1 B1DKG2_9BACL Metallophosphoesterase Length = 479 Score = 191 bits (484), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 118/271 (43%), Positives = 159/271 (58%), Gaps = 3/271 (1%) Query: 1 MLETPQMINGTAVCGAGKFGRYIGRLIFERAHAGAAFELVSGRCFTVDPSLTEDVLAPAA 60 +LE P +I GT++C AGK+G IGR+ E + A ++ C + A Sbjct: 207 LLEEPLVIGGTSICAAGKYGEQIGRVELELDES-AKRPIIRAACVPMAAYAEHSAAAAII 265 Query: 61 APHLQHGREVLNETVAITDRELTLDTEGESPFGNLLAQAVRRFTGAPISLVNSGQLLGPL 120 + + G L+ +A D L E ESP GNLLA +RR A I +VN+GQLLG L Sbjct: 266 GGYREAGERRLSRVIARLDAPLPALPERESPLGNLLAAGLRRHADAEIGIVNTGQLLGGL 325 Query: 121 PQGTITAGMLHALCPSPVNACIIRLKGEDIRKALEQSRMEAFYNKAIFGYGFRGKVLGSL 180 QG +T G LHALCPSP+N C + L G +IR ALE++ + +F I GYGFRG+VLG+L Sbjct: 326 QQGDVTEGQLHALCPSPINPCRMLLSGANIRAALEEALLPSFTGMPIKGYGFRGEVLGTL 385 Query: 181 AVDGLKILYDPGVLPYDNGNGIAVFVGGEPLDDKAVYSVGTLDMFTFRIGYESLADGTEP 240 A+DG+++ YDP LP + +++ V GE L D+ Y VG +DMFTF IGY SL TE Sbjct: 386 AIDGIEVEYDPS-LP-ELHRIVSIHVNGELLSDEREYVVGCIDMFTFHIGYLSLGQATEV 443 Query: 241 LYLLPHFLRDLLRMELQRPGSLDECAGLRWK 271 Y LP F+RDLL EL+ + C RW+ Sbjct: 444 SYYLPEFIRDLLAAELREGHGITACHQERWR 474 >C0ZKY6.1 C0ZKY6_BREBN Putative nucleotidase EC=3.1.3.- Length = 484 Score = 145 bits (365), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 87/275 (31%), Positives = 144/275 (52%), Gaps = 16/275 (5%) Query: 6 QMINGTAVCGAGKFGRYIGR--LIFERAHAG---AAFELVSGRCFTVDPSLTEDVLAPAA 60 + I GT + AG+FG Y+G L+++R G EL + VD SL++ + + Sbjct: 213 ERIGGTLITQAGRFGEYVGHVELVWDRTQNGIKDVRAELFHTEQYQVDESLSQFIESEKV 272 Query: 61 APHLQHGREVLNETVAITDRELTLDTEGESPFGNLLAQAVRRFTGAPISLVNSGQLLGPL 120 L + +A +++L + E+P+G+ +A ++R++TGA I + N G LL L Sbjct: 273 W-----AENRLFQPIAQLEQDLHIGWTEETPYGSFIAASIRKWTGAEIGMANGGLLLADL 327 Query: 121 PQGTIT-AGMLHALCPSPVNACIIRLKGEDIRKALEQSRMEAFYNKAIFGYGFRGKVLGS 179 +G+++ A +LH++ P P+N C + + G + LEQ+ + + GYGFRGK+ G Sbjct: 328 ARGSLSFADLLHSM-PHPINPCAVTITGAQLTTVLEQAIQPEMIQRELRGYGFRGKIEGW 386 Query: 180 LAVDGLKILYDPGVLPYDNGNGIAVFVGGEPLDDKAVYSVGTLDMFTFRIGYESLADGTE 239 + VDGL I Y D I + V G+PLD + +Y VGT+DMF + + L G+E Sbjct: 387 MCVDGLHIRYSED----DQPQIIQIEVNGQPLDRERLYRVGTIDMFMYNRMFPELLLGSE 442 Query: 240 PLYLLPHFLRDLLRMELQRPGSLDECAGLRWKNGS 274 + LP LR++L ++ L RW+ S Sbjct: 443 LTFFLPEMLREVLATTIKDQQMLQNAFLPRWEKIS 477 >Q5KVL8.1 Q5KVL8_GEOKA Hypothetical conserved protein Length = 460 Score = 145 bits (365), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 84/252 (33%), Positives = 130/252 (51%), Gaps = 5/252 (1%) Query: 6 QMINGTAVCGAGKFGRYIGRLIFERAHAGAAFELVSGRCFTVDP-SLTEDVLAPAAAPHL 64 + +N T +C AGK+G+Y+G + E S V+P +E V Sbjct: 211 KTLNQTLLCAAGKYGKYVGVVKLEIDQN--RLSRASATVVDVEPLPRSEAVERHLVCLEQ 268 Query: 65 QHGREVLNETVAITDRELTLDTEGESPFGNLLAQAVRRFTGAPISLVNSGQLLGPLPQGT 124 + + E VA+ +L LD SP GNLLA A+R + A I +VN+G LL PL +G Sbjct: 269 ESLARLEAEQVAVLHDDLPLDWFAPSPLGNLLASALREWCQADIGMVNAGVLLAPLAKGP 328 Query: 125 ITAGMLHALCPSPVNACIIRLKGEDIRKALEQSRMEAFYNKAIFGYGFRGKVLGSLAVDG 184 +T LH +CP P+N C ++L+G ++++ + ++ E + G+GFRG+V+G + DG Sbjct: 329 VTKKDLHRICPHPLNPCKVKLRGAELKEIILEANTERMKHWQFKGFGFRGEVMGEMVYDG 388 Query: 185 LKILYDPGVLPYDNGNGIAVFVGGEPLDDKAVYSVGTLDMFTFRIGYESLADGTEPLYLL 244 + I + G+ + + + GEPLD + Y V T DMF Y + E Y + Sbjct: 389 VDI--EAGLEEDGQWHIRRIMINGEPLDPERTYEVATTDMFAIGHFYPQIQRAAEKTYYM 446 Query: 245 PHFLRDLLRMEL 256 P FLRDLL +L Sbjct: 447 PEFLRDLLAWKL 458 >Q815Z7.1 Q815Z7_BACCR 5'-nucleotidase EC=3.1.3.5 Length = 426 Score = 142 bits (359), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 81/262 (30%), Positives = 138/262 (52%), Gaps = 22/262 (8%) Query: 1 MLETPQMINGTAVCGAGKFGRYIGRLIFERAHAGAAFELVSGRCFTVD-------PSLTE 53 + E ++N T +C K+GRY+G + GR + P T Sbjct: 164 LFERGVLMNNTLLCCCEKWGRYVGHVQLTVDKETKKLLKKDGRAIKAERLGAYSEPLSTI 223 Query: 54 DVLAPAAAPHLQHGREVLNETVAITDRELTLDTEGESPFGNLLAQAVRRFTGAPISLVNS 113 ++L + + ++ E V L +D E+PF ++LA A++++ GA I +VN+ Sbjct: 224 EILQ-------EESKHIMAEPVVHLKESLQVDWFHETPFSHMLASALKKWCGAEIGMVNA 276 Query: 114 GQLLGPLPQGTITAGMLHALCPSPVNACIIRLKGEDIRKALEQSRMEAFYNKAIFGYGFR 173 G LL L +G +T G +H +CP P+N C +++ G+ +R+ + ++R N + G+GFR Sbjct: 277 GVLLEGLEEGVVTRGDIHRICPHPINPCALKVPGKILREVILKARKPNMENLEVKGFGFR 336 Query: 174 GKVLGSLAVDGLKILYDPGVLPYDNGNGIA---VFVGGEPLDDKAVYSVGTLDMFTFRIG 230 GKV+G + G++++ P +P GN I V + GE L+ +Y+VGT+DMFTF Sbjct: 337 GKVMGKMIYAGVEVI--PDTIP---GNKILLEDVLINGESLELDRIYTVGTIDMFTFGYL 391 Query: 231 YESLADGTEPLYLLPHFLRDLL 252 Y L+ ++ Y +P LRD+L Sbjct: 392 YPELSTLSDKQYYMPELLRDVL 413 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.141 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,762,199,978 Number of extensions: 77885242 Number of successful extensions: 167415 Number of sequences better than 1.0e-05: 244 Number of HSP's gapped: 167146 Number of HSP's successfully gapped: 244 Length of query: 275 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 141 Effective length of database: 1,798,280,193 Effective search space: 253557507213 Effective search space used: 253557507213 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)