BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0156.0008.p (296 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K646.1 B3K646_9BACI ROK family protein 441 e-122 B1DFB9.1 B1DFB9_9BACL ROK family protein 409 e-112 B3KI95.1 B3KI95_9BACI ROK family protein 380 e-103 Q5KUZ7.1 Q5KUZ7_GEOKA Fructokinases EC=2.7.1.4 348 2e-94 C3J4H9.1 C3J4H9_9BACI ROK family protein 346 2e-93 >B3K646.1 B3K646_9BACI ROK family protein Length = 304 Score = 441 bits (1135), Expect = e-122, Method: Compositional matrix adjust. Identities = 212/293 (72%), Positives = 243/293 (82%) Query: 1 MIIGAIEAGGTKFICGVGNEEGEILDRISFPTETPEETLQQAVAYFKDTGVEAIGIGSFG 60 M IGAIEAGGTKFICG+GNEEGEILDRISFPT+ PE TL+Q VAYF+D +EAIG+GSFG Sbjct: 1 MRIGAIEAGGTKFICGIGNEEGEILDRISFPTDIPEPTLEQVVAYFRDKDIEAIGVGSFG 60 Query: 61 PLNMDFSSPLYGHVTTTPKRGWGGFDFVGYMKRHFPVPVGFDTDVNAAAFGEVKWGAAQG 120 P+++D SS YG VTTTPK GW GFDFVG+MKR+FPVP+GFDTDVNAA FGE WGAA+G Sbjct: 61 PIDLDRSSLTYGQVTTTPKPGWSGFDFVGFMKRNFPVPIGFDTDVNAAVFGEAMWGAAKG 120 Query: 121 SDSCXXXXXXXXXXXXXXAEGRLVHGLVHPEGGHVLTRRHAEDVFEGLCPYHGDCLEGLA 180 DSC AEG L+HGLVHPEGGHVLTRRH ED FEG CPYHGDCLEG+A Sbjct: 121 LDSCVYYTIGTGVGVGVFAEGNLIHGLVHPEGGHVLTRRHPEDPFEGFCPYHGDCLEGMA 180 Query: 181 AGPAIEKRWGVKGSALPADHPAWEMEAYYLGQAIAGVVLLLSPKRVILGGGVMHREQLFS 240 +GPAIEKRWG+KGS LP DH AWEME +YL QAI+GV+LLLSPK+VILGGGVMH++QL+ Sbjct: 181 SGPAIEKRWGLKGSELPEDHLAWEMEGFYLAQAISGVILLLSPKKVILGGGVMHQKQLYP 240 Query: 241 LIRKEVRQALNGYVAAPEILEAMDTYIVPPGLGDHAGLCGALALGIEALQGPA 293 LIRK+V + LNGYVA+P IL+ ++ YIV PGLGD+AGLCGALALGIEA A Sbjct: 241 LIRKKVAKFLNGYVASPAILDDLENYIVSPGLGDNAGLCGALALGIEASSSKA 293 >B1DFB9.1 B1DFB9_9BACL ROK family protein Length = 293 Score = 409 bits (1051), Expect = e-112, Method: Compositional matrix adjust. Identities = 193/290 (66%), Positives = 233/290 (80%) Query: 1 MIIGAIEAGGTKFICGVGNEEGEILDRISFPTETPEETLQQAVAYFKDTGVEAIGIGSFG 60 M IGAIEAGGTKF+CG+GNE GEI +RIS PTE P+ TL++ + YF+D VEAIGIG+FG Sbjct: 1 MRIGAIEAGGTKFVCGIGNEHGEIEERISLPTEHPDSTLREVLNYFRDKQVEAIGIGTFG 60 Query: 61 PLNMDFSSPLYGHVTTTPKRGWGGFDFVGYMKRHFPVPVGFDTDVNAAAFGEVKWGAAQG 120 P+++D SSP YG+VTTTPK GW G+DF+G MKR PVP G+DTDVNAAAFGE KWGAA+G Sbjct: 61 PIDIDPSSPTYGYVTTTPKPGWSGYDFLGTMKRELPVPYGWDTDVNAAAFGEAKWGAAKG 120 Query: 121 SDSCXXXXXXXXXXXXXXAEGRLVHGLVHPEGGHVLTRRHAEDVFEGLCPYHGDCLEGLA 180 +S EG+LVHGL+HPEGGHVLTRRH ED F G CPYHGDCLEG+A Sbjct: 121 LNSALYYTVGTGIGVGVYTEGKLVHGLLHPEGGHVLTRRHPEDTFAGFCPYHGDCLEGMA 180 Query: 181 AGPAIEKRWGVKGSALPADHPAWEMEAYYLGQAIAGVVLLLSPKRVILGGGVMHREQLFS 240 AGPAI +RWGVK L DHPAW+MEA+YLGQAI+ +LLLSPK+VILGGGVMH+EQLF Sbjct: 181 AGPAIGQRWGVKADELTKDHPAWQMEAFYLGQAISSAILLLSPKKVILGGGVMHQEQLFP 240 Query: 241 LIRKEVRQALNGYVAAPEILEAMDTYIVPPGLGDHAGLCGALALGIEALQ 290 L+R EV++ LNGY + + E +D+YIVPPGLGD+AGLCG++ALGI+AL+ Sbjct: 241 LVRAEVQKNLNGYASHEALREGIDSYIVPPGLGDNAGLCGSIALGIDALE 290 >B3KI95.1 B3KI95_9BACI ROK family protein Length = 294 Score = 380 bits (975), Expect = e-103, Method: Compositional matrix adjust. Identities = 183/290 (63%), Positives = 219/290 (75%) Query: 1 MIIGAIEAGGTKFICGVGNEEGEILDRISFPTETPEETLQQAVAYFKDTGVEAIGIGSFG 60 M IGA+EAGGTKF+CG+GNE G + DRISFPT PE TL +A++YF + VEAIGIGSFG Sbjct: 1 MRIGAMEAGGTKFVCGIGNENGIVEDRISFPTGQPEGTLSRAISYFHEHEVEAIGIGSFG 60 Query: 61 PLNMDFSSPLYGHVTTTPKRGWGGFDFVGYMKRHFPVPVGFDTDVNAAAFGEVKWGAAQG 120 P+++ SP YG+VT+TPK GW D +G MK H +P G+DTDVNAAA GE WGAA+G Sbjct: 61 PIDLKPDSPTYGYVTSTPKHGWSQIDVLGNMKSHMDIPYGWDTDVNAAALGEATWGAAKG 120 Query: 121 SDSCXXXXXXXXXXXXXXAEGRLVHGLVHPEGGHVLTRRHAEDVFEGLCPYHGDCLEGLA 180 +SC AEGRLVHGLVHPEGGHV +RH ED F G CPYHGDCLEGLA Sbjct: 121 LNSCVYYTVGTGIGLGLYAEGRLVHGLVHPEGGHVPVKRHPEDAFAGTCPYHGDCLEGLA 180 Query: 181 AGPAIEKRWGVKGSALPADHPAWEMEAYYLGQAIAGVVLLLSPKRVILGGGVMHREQLFS 240 AGPA+E RWG+KG LPADHPAWE+EAYY+ Q+I G++L SP+++ILGGGVM + QL+ Sbjct: 181 AGPALEARWGLKGHLLPADHPAWEIEAYYIAQSITGLILTNSPEKIILGGGVMQQAQLYP 240 Query: 241 LIRKEVRQALNGYVAAPEILEAMDTYIVPPGLGDHAGLCGALALGIEALQ 290 LIR EVR+ LNGYV A EI+ ++ YIVPP LG +GLCGALALGI A Q Sbjct: 241 LIRAEVRRNLNGYVLADEIVHDIEKYIVPPVLGTQSGLCGALALGIRAWQ 290 >Q5KUZ7.1 Q5KUZ7_GEOKA Fructokinases EC=2.7.1.4 Length = 290 Score = 348 bits (894), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 159/275 (57%), Positives = 205/275 (74%) Query: 1 MIIGAIEAGGTKFICGVGNEEGEILDRISFPTETPEETLQQAVAYFKDTGVEAIGIGSFG 60 MI+GAIEAGGTKF+C +G+E+G I +R FPT PEET+ + +F+ +EAIGIGSFG Sbjct: 1 MILGAIEAGGTKFVCAIGDEQGNIHERAVFPTTAPEETMAHVIDFFRPHRIEAIGIGSFG 60 Query: 61 PLNMDFSSPLYGHVTTTPKRGWGGFDFVGYMKRHFPVPVGFDTDVNAAAFGEVKWGAAQG 120 P+++ SP YG++T+TPK+ W FDFVG MK+HFPVP+GFDTDVNAAA GE++WGAAQG Sbjct: 61 PVDLRPDSPTYGYITSTPKQAWAQFDFVGTMKQHFPVPIGFDTDVNAAALGELRWGAAQG 120 Query: 121 SDSCXXXXXXXXXXXXXXAEGRLVHGLVHPEGGHVLTRRHAEDVFEGLCPYHGDCLEGLA 180 DSC EGRL+HG++HPE GH+ RRH +D F G+CPYHGDCLEG+A Sbjct: 121 LDSCLYMTVGTGIGVGAVVEGRLLHGMLHPEMGHMFVRRHPDDAFAGICPYHGDCLEGMA 180 Query: 181 AGPAIEKRWGVKGSALPADHPAWEMEAYYLGQAIAGVVLLLSPKRVILGGGVMHREQLFS 240 +GPAIE+RWG KG+ L H WE+EA+YL QAIA +L+LSP++VI GGGVM + + Sbjct: 181 SGPAIERRWGKKGAELADRHEVWELEAFYLAQAIANYILVLSPEKVITGGGVMKQTHVLP 240 Query: 241 LIRKEVRQALNGYVAAPEILEAMDTYIVPPGLGDH 275 L+R+ V++ LNGYV ILE +D YIV PGLGD+ Sbjct: 241 LVRRHVQELLNGYVQHETILEKIDEYIVLPGLGDN 275 >C3J4H9.1 C3J4H9_9BACI ROK family protein Length = 290 Score = 346 bits (887), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 159/275 (57%), Positives = 204/275 (74%) Query: 1 MIIGAIEAGGTKFICGVGNEEGEILDRISFPTETPEETLQQAVAYFKDTGVEAIGIGSFG 60 MI+GAIEAGGTKF+C +G+E+G I +R FPT PEET+ + +F+ +EAIGIGSFG Sbjct: 1 MILGAIEAGGTKFVCAIGDEQGNIHERAVFPTTAPEETMAHVIDFFRPHRIEAIGIGSFG 60 Query: 61 PLNMDFSSPLYGHVTTTPKRGWGGFDFVGYMKRHFPVPVGFDTDVNAAAFGEVKWGAAQG 120 P+++ SP YG++T+TPK+ W FDFVG MK+HFPVP+GFDTDVNAAA GE +WGAA+G Sbjct: 61 PVDLRPDSPTYGYITSTPKQAWAQFDFVGTMKQHFPVPIGFDTDVNAAALGEQRWGAARG 120 Query: 121 SDSCXXXXXXXXXXXXXXAEGRLVHGLVHPEGGHVLTRRHAEDVFEGLCPYHGDCLEGLA 180 DSC EGRL+HGL+HPE GH+L RRH +D F G+CPYHGDCLEG+A Sbjct: 121 LDSCLYMTVGTGIGVGAIIEGRLLHGLLHPEMGHMLVRRHPDDAFAGICPYHGDCLEGMA 180 Query: 181 AGPAIEKRWGVKGSALPADHPAWEMEAYYLGQAIAGVVLLLSPKRVILGGGVMHREQLFS 240 +GPAIE+RWG KG+ L H WE+EA+YL QAIA +L+LSPK+VI GGGVM + + Sbjct: 181 SGPAIERRWGKKGAELADRHEVWELEAFYLAQAIANYILILSPKKVITGGGVMKQTHVLP 240 Query: 241 LIRKEVRQALNGYVAAPEILEAMDTYIVPPGLGDH 275 L+R+ V++ L GY+ ILE +D YIV PGLGD+ Sbjct: 241 LVRRHVQELLGGYIQHEAILEKIDEYIVLPGLGDN 275 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.141 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,906,059,038 Number of extensions: 82457100 Number of successful extensions: 165669 Number of sequences better than 1.0e-05: 578 Number of HSP's gapped: 165273 Number of HSP's successfully gapped: 578 Length of query: 296 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 161 Effective length of database: 1,788,859,178 Effective search space: 288006327658 Effective search space used: 288006327658 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)