BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0156.0001.p (330 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K653.1 B3K653_9BACI Regulatory protein LacI 549 e-154 B3KCU1.1 B3KCU1_9BACI Periplasmic binding protein/LacI transcrip... 148 9e-34 B9R3G4.1 B9R3G4_9RHOB Periplasmic binding proteins and sugar bin... 140 1e-31 B9LAQ6.1 B9LAQ6_CHLSY Transcriptional regulator, LacI family EC=... 140 1e-31 A8LIQ1.1 A8LIQ1_DINSH Transcriptional regulator, LacI family EC=... 137 1e-30 >B3K653.1 B3K653_9BACI Regulatory protein LacI Length = 330 Score = 549 bits (1414), Expect = e-154, Method: Compositional matrix adjust. Identities = 257/330 (77%), Positives = 300/330 (90%) Query: 1 MAKQRVTLVQVAKDAGVSPATVSHFMNGNFHRMRPETREKISDSITKLGYQANPVAKSLI 60 MA +RVTLVQVAKDAGVSPATVSHF+NGNF +M ET+EKIS SITKLGYQ NPVAKSLI Sbjct: 1 MANKRVTLVQVAKDAGVSPATVSHFLNGNFQKMGSETKEKISLSITKLGYQVNPVAKSLI 60 Query: 61 TGKMYTIGLVLSNSTYDGYFEDLYFLHFAKVLKQQLKTMGYKLILLDFDEIFMNIQMVDG 120 TG+M+TIGLVLS+ST DGYFEDLYFLHFA+V+K QLK +GYK+ILLDF+EIFMNIQMVDG Sbjct: 61 TGRMHTIGLVLSDSTDDGYFEDLYFLHFAQVIKHQLKALGYKIILLDFEEIFMNIQMVDG 120 Query: 121 IIVKATLETEKFMPRLMDLNVPVITIGRHNLSYGAHIVRVNDYQCGQTGVDHLMSRGYHK 180 IIVKAT++TEKFMPRLM+LNVPVITIGRHNLSYGAH++RV+DY GQTGV+HL+SRGY Sbjct: 121 IIVKATMDTEKFMPRLMNLNVPVITIGRHNLSYGAHMLRVDDYLSGQTGVNHLLSRGYQN 180 Query: 181 IIILTYPHGQVPGFDDRLNGASQAMQEKGNLTTVITGDMTESFGYDTVMQLHSNGQLPQA 240 ++ILTYPHG VPGFDDRLNGAS+A+++ G LTTVITGDMTE+FGYDTV+ L N QLPQA Sbjct: 181 LVILTYPHGHVPGFDDRLNGASEALRQNGKLTTVITGDMTETFGYDTVVNLVQNNQLPQA 240 Query: 241 LFCLNDISAIGVLKACKDLNLSVPENLAVLGVDDMPTVSELLSLSTIRHPINELALNASE 300 LFCLNDISAIG LKACKDLN+S+P+ LAVLGVDDMPTVSELLSLSTIRHPIN+LA A++ Sbjct: 241 LFCLNDISAIGALKACKDLNISIPDKLAVLGVDDMPTVSELLSLSTIRHPINDLAETAAK 300 Query: 301 LIIQSVETKGEPVQPVDRMFPIELMARHTS 330 L+I+S+E+ G+ ++PV+ +FP ELM R TS Sbjct: 301 LMIKSIESDGQRIEPVEEVFPTELMVRRTS 330 >B3KCU1.1 B3KCU1_9BACI Periplasmic binding protein/LacI transcriptional regulator Length = 336 Score = 148 bits (373), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 96/340 (28%), Positives = 178/340 (52%), Gaps = 25/340 (7%) Query: 5 RVTLVQVAKDAGVSPATVSHFMNGNFHRMRPETREKISDSITKLGYQANPVAKSLITGKM 64 R T+ VA++AGVS ATVSH +NG R E RE+I D + +L Y+ N +A +L + Sbjct: 3 RATIYDVAREAGVSIATVSHVINGKGKISR-ERREEILDIMKRLNYRPNAIASALAGKRT 61 Query: 65 YTIGLVLSNSTYDGYFEDLYFLHFAKVLKQQLKTMGYKLILLDFD------EIFMNI--- 115 +T+GL++ + + +F A+ ++ Q +GY +I+ D + ++N+ Sbjct: 62 FTLGLLVPD------ISNPFFAEMARSVEDQGHQLGYSVIICSTDNQEEKVKRYVNLLRQ 115 Query: 116 QMVDGIIVKATLETEKFMPRLMDLNVPVITIGRHNLSYGAHIVRVNDYQCGQTGVDHLMS 175 + VDG+I+ + + + L + ++ ++ I R N + A VRV+D + G+ +HL+ Sbjct: 116 KQVDGVIIGTGISHGELLKPLQEHSLAIVLIARDNPALDASSVRVHDLEGGRLAAEHLLQ 175 Query: 176 RGYHKIIILTYPHGQVPGFDDRLNGASQAMQEKG---NLTTVITGDMTESFGYDTVMQLH 232 G+ ++ +L + V +R+ G +A G + + D GY + Sbjct: 176 LGHRRLAVLAE-NEWVTSSSERVRGFVEAAASHGLELSPGCIAACDHKIEAGYRRAKAML 234 Query: 233 SNGQLPQALFCLNDISAIGVLKACKDLNLSVPENLAVLGVDD--MPTVSELLSLSTIRHP 290 P ALFC ND+ A+G L+A ++L L VPE +V+ DD + +V++ L+ I P Sbjct: 235 ELAVRPTALFCCNDLLAVGALRAARELGLRVPEECSVVSFDDTILASVTD-PPLTVIAQP 293 Query: 291 INELALNASELIIQSVETKGEPVQPVDRMFPIELMARHTS 330 I ++ A +L+++ + KG+P + + + P EL+ R ++ Sbjct: 294 IEQMGRMAVDLVVRGLSGKGDPARRI--VLPPELIVRQST 331 >B9R3G4.1 B9R3G4_9RHOB Periplasmic binding proteins and sugar binding domain of the LacI family, putative Length = 376 Score = 140 bits (354), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 102/338 (30%), Positives = 179/338 (52%), Gaps = 26/338 (7%) Query: 6 VTLVQVAKDAGVSPATVSH-FMNGNFHRMRPETREKISDSITKLGYQANPVAKSLITGKM 64 VTL +VA+ AGVS + VS F +G + +TR K+ + TKLGY N +A+ L T + Sbjct: 3 VTLKEVAELAGVSRSAVSRTFTDG--ASVSDKTRRKVEKAATKLGYTPNILARGLTTRRT 60 Query: 65 YTIGLVLSNSTYDGYFEDLYFLHFAKVLKQQLKTMGYKLILLDF-DEI--FMNIQM---- 117 IGLV +N F + FL ++L+ +G + +L++ DE +++M Sbjct: 61 KLIGLVSNN------FHNPIFLEVFDQFTRKLQGVGLRPLLVNLSDETDPEASVRMLRQY 114 Query: 118 -VDGIIVKATLETEKFMPRLMDLNVPVI-TIGRHNLSYGAHIVRVNDYQCGQTGVDHLMS 175 VDG++V ++ F D VPV+ + GR++ + H+V +++ +CG+ L Sbjct: 115 SVDGVVVASSTLPPGFSKAFKDAGVPVVNSFGRYSTAPEFHVVGIDNVECGRMAALELAK 174 Query: 176 RGYHKIIILTYPHGQVPGFDDRLNGASQAMQEKGNLTTVITGDMTESF--GYDTVMQLHS 233 RGY + L P DR G S+ + ++ ++ SF G + + +L Sbjct: 175 RGYKHVAFLGGPD-TATSTQDRFKGFSEEISRHPDMEMTVSYAKAYSFEAGREEMQRLLG 233 Query: 234 NGQLPQALFCLNDISAIGVLKACKDLNLSVPENLAVLGVDDMPTVS-ELLSLSTIRHPIN 292 L +A FC +D+ +IGVL A +D L++P ++ ++G++DM + ++L+TIR P+ Sbjct: 234 IRPLAEAYFCGDDVLSIGVLSAIEDAGLNIPGDIGIIGLNDMEMAGWQNINLTTIRQPVQ 293 Query: 293 ELALNASELIIQSVETKGEPVQ-PVDRMFPIELMARHT 329 ++ ++ ELI+ +E EP + P R+FP E++ R T Sbjct: 294 QIINSSVELIVSMLE---EPARYPEARLFPCEVIERGT 328 >B9LAQ6.1 B9LAQ6_CHLSY Transcriptional regulator, LacI family EC=5.1.1.1 Length = 342 Score = 140 bits (354), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 97/339 (28%), Positives = 165/339 (48%), Gaps = 22/339 (6%) Query: 6 VTLVQVAKDAGVSPATVSHFMNGNFHRMRPETREKISDSITKLGYQANPVAKSLITGKMY 65 +TL ++A+ AGVS +T S +N + H + P RE++ +I + YQ N A+SL + + Sbjct: 3 ITLDEIARRAGVSRSTASRVLNNHPH-VNPAVRERVWQAIRETNYQPNHPARSLASRRSQ 61 Query: 66 TIGLVLSNSTYDGYFEDLYFLHFAKVLKQQLKTMGYKLILLDFDEIFMNIQ--------- 116 IG+++ S + F D YF + + + ++L E+ N Sbjct: 62 VIGIIIPQSIHT-LFADPYFPLLIEGIACVCNAREFSIML----ELATNTTGNPYRHIGR 116 Query: 117 --MVDGIIVKATLETEKFMPRLMDLNVPVITIGRHNLSYGAHIVRVNDYQCGQTGVDHLM 174 +DG IV + +E E L +PV+++GR + V V++ + VDHL+ Sbjct: 117 RGFLDGAIVASAIEDEFLAQWLQQETLPVVSVGRISSHPVIPYVDVDNRHGARMMVDHLI 176 Query: 175 SRGYHKIIILTYPHGQVPGFDDRLNGASQAMQEKG---NLTTVITGDMTESFGYDTVMQL 231 RGY +I +T P V G DR G + + G V+ G+ +E G+ + L Sbjct: 177 RRGYRRIATITGPMNIVAG-RDRYAGYCEGLAAAGLPVQPELVVEGNFSEQSGFAAMQML 235 Query: 232 HSNGQLPQALFCLNDISAIGVLKACKDLNLSVPENLAVLGVDDMPTVSELL-SLSTIRHP 290 LP A+F +D+ A+G KA + +L VP ++A+ G DD+P + + +L+T+R P Sbjct: 236 LQLDPLPDAVFAASDMMALGAFKALRAADLVVPRDIALTGFDDIPLAAAMTPALTTVRQP 295 Query: 291 INELALNASELIIQSVETKGEPVQPVDRMFPIELMARHT 329 INEL A+ L++ + P + P EL+ R + Sbjct: 296 INELGQTAARLLLDHLIIASSEPAPQRVILPTELIIRES 334 >A8LIQ1.1 A8LIQ1_DINSH Transcriptional regulator, LacI family EC=5.1.1.1 Length = 332 Score = 137 bits (345), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 102/338 (30%), Positives = 173/338 (51%), Gaps = 27/338 (7%) Query: 6 VTLVQVAKDAGVSPATVSH-FMNGNFHRMRPETREKISDSITKLGYQANPVAKSLITGKM 64 VTL +VA+ AGVS + VS F G + P+TR K+ + +LGY+ N +A SL TG+ Sbjct: 3 VTLKEVAERAGVSRSAVSRTFTEG--ASVSPKTRRKVEKAAVELGYRPNALASSLTTGRT 60 Query: 65 YTIGLVLSNSTYDGYFEDLYFLHFAKVLKQQLKTMGYKLILLDFDE-------IFMNIQM 117 IGL+ +N F + FL + + L+ G + +L++ + I M Q Sbjct: 61 KLIGLISNN------FRNPLFLEVFDLFTRLLQERGLRPLLVNLSDENDPENSISMLRQY 114 Query: 118 -VDGIIVKATLETEKFMPRLMDLNVPVI-TIGRHNLSYGAHIVRVNDYQCGQTGVDHLMS 175 VDG+IV ++ F D +PV+ + GR + H+V +++ +CG+ L++ Sbjct: 115 SVDGVIVASSTLPPSFSKAFRDAGIPVVHSFGRFASAPHVHVVGIDNIECGRMAARTLLA 174 Query: 176 RGYHKIIILTYPHGQVPGFDDRLNGASQAMQEKGNLTTVITGDMTESF--GYDTVMQLHS 233 RGYHKI + P + DR G Q + + + + SF G + +L Sbjct: 175 RGYHKIGFMGGPR-EATSTIDRREGFHQVVARRNGIEVSESFAAAYSFDAGKTEMSRLLQ 233 Query: 234 NGQLPQALFCLNDISAIGVLKACKDLNLSVPENLAVLGVDDMPTVSEL-LSLSTIRHPIN 292 +G +A FC +D+ +IG L A + L VP+++ ++G++DM L L+TIR P+ Sbjct: 234 HGPA-EAYFCGDDVLSIGALAAIEAAGLRVPQDVGIIGLNDMEMAGWSNLDLTTIRQPVQ 292 Query: 293 ELALNASELIIQSVETKGEPVQ-PVDRMFPIELMARHT 329 ++ ++ EL++ +E EP + P R+F E++ R T Sbjct: 293 QIISSSIELVVAMLE---EPDRYPEARLFACEVIERGT 327 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,867,175,919 Number of extensions: 74482463 Number of successful extensions: 248888 Number of sequences better than 1.0e-05: 7533 Number of HSP's gapped: 237753 Number of HSP's successfully gapped: 7546 Length of query: 330 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 194 Effective length of database: 1,779,438,163 Effective search space: 345211003622 Effective search space used: 345211003622 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)