BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0154.0001.p (176 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9K9X4.1 Q9K9X4_BACHD Transposase related protein (20) 258 1e-67 Q75U24.1 Q75U24_BACHD Transposase B of IS642 258 2e-67 Q75TQ2.1 Q75TQ2_BACAO Transposase B of IS642 (Fragment) 243 5e-63 B7GF49.1 B7GF49_ANOFW Transposase 238 2e-61 B7GF72.1 B7GF72_ANOFW Transposase 236 6e-61 >Q9K9X4.1 Q9K9X4_BACHD Transposase related protein (20) Length = 188 Score = 258 bits (660), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 118/176 (67%), Positives = 144/176 (81%) Query: 1 MKDEIHHLLFEDESMIRDYQAIQKTWFLRGKQRIIPTTGKHRGVKLLATVDYGTGKIVWQ 60 + +EI HLLF+DESMIRDYQA+Q TWFL+GKQRIIPT GKH+GVKL+ T++Y TG ++ Sbjct: 13 LNEEIDHLLFQDESMIRDYQALQYTWFLKGKQRIIPTYGKHQGVKLIGTLNYETGDVLCV 72 Query: 61 EDEQYTAETFLSFLKKVIDEYPTGKIVMVLDNARIHHAELLTPFLTEMKERLELVFLPPY 120 E+E+Y AE FL FL+KV+ YPTGKIVMVLDNARIHHA+L+ PFL + RLELVFLPPY Sbjct: 73 EEERYDAEAFLRFLQKVLKHYPTGKIVMVLDNARIHHAKLIEPFLRNHQHRLELVFLPPY 132 Query: 121 SPQFNAVEGLWKWLKSDVINNVFYHTVTEIRTNVQQFMEEIMKVPLTIIDRLCVRF 176 SP+ N +EGLWKWLKSDVINN+FY +V EIR NVQ F+ E+ P +IDRLC+R Sbjct: 133 SPELNLIEGLWKWLKSDVINNLFYSSVKEIRRNVQAFIRELNDSPRKVIDRLCIRL 188 >Q75U24.1 Q75U24_BACHD Transposase B of IS642 Length = 188 Score = 258 bits (658), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 117/176 (66%), Positives = 144/176 (81%) Query: 1 MKDEIHHLLFEDESMIRDYQAIQKTWFLRGKQRIIPTTGKHRGVKLLATVDYGTGKIVWQ 60 + +EI HLLF+DESMIRDYQA+Q TWFL+GKQRIIPT GKH+GVKL+ T++Y TG ++ Sbjct: 13 LNEEIDHLLFQDESMIRDYQALQYTWFLKGKQRIIPTYGKHQGVKLIGTLNYETGDVLCV 72 Query: 61 EDEQYTAETFLSFLKKVIDEYPTGKIVMVLDNARIHHAELLTPFLTEMKERLELVFLPPY 120 E+E+Y AE FL FL+KV+ YPTGKIVMVLDNARIHHA+L+ PFL + RLELVFLPPY Sbjct: 73 EEERYDAEAFLRFLQKVLKHYPTGKIVMVLDNARIHHAKLIEPFLRNHQHRLELVFLPPY 132 Query: 121 SPQFNAVEGLWKWLKSDVINNVFYHTVTEIRTNVQQFMEEIMKVPLTIIDRLCVRF 176 SP+ N +EGLWKWL+SDVINN+FY +V EIR NVQ F+ E+ P +IDRLC+R Sbjct: 133 SPELNLIEGLWKWLRSDVINNLFYSSVKEIRRNVQAFIRELNDSPRKVIDRLCIRL 188 >Q75TQ2.1 Q75TQ2_BACAO Transposase B of IS642 (Fragment) Length = 177 Score = 243 bits (620), Expect = 5e-63, Method: Compositional matrix adjust. Identities = 112/165 (67%), Positives = 136/165 (82%) Query: 1 MKDEIHHLLFEDESMIRDYQAIQKTWFLRGKQRIIPTTGKHRGVKLLATVDYGTGKIVWQ 60 + +EI HLLF+DESMIRDYQA+Q TWFL+GKQRIIPT GKH+GVKL+ T++Y TG ++ Sbjct: 13 LNEEIDHLLFQDESMIRDYQALQYTWFLKGKQRIIPTYGKHQGVKLIGTLNYETGDVLCV 72 Query: 61 EDEQYTAETFLSFLKKVIDEYPTGKIVMVLDNARIHHAELLTPFLTEMKERLELVFLPPY 120 E+E+Y AE FL FL+KV+ YPTGKIVMVLDNARIHHA+L+ PFL + RLELVFLPPY Sbjct: 73 EEERYDAEAFLRFLQKVLKHYPTGKIVMVLDNARIHHAKLIEPFLRNHQHRLELVFLPPY 132 Query: 121 SPQFNAVEGLWKWLKSDVINNVFYHTVTEIRTNVQQFMEEIMKVP 165 SP+ N +EGLWKWLKSDVINN+FY +V EIR NVQ F+ E+ P Sbjct: 133 SPELNLIEGLWKWLKSDVINNLFYSSVREIRRNVQAFIRELNDSP 177 >B7GF49.1 B7GF49_ANOFW Transposase Length = 162 Score = 238 bits (606), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 110/162 (67%), Positives = 135/162 (83%) Query: 15 MIRDYQAIQKTWFLRGKQRIIPTTGKHRGVKLLATVDYGTGKIVWQEDEQYTAETFLSFL 74 MIRDYQAIQKTWF++GKQRIIPT GKH+G+KL+ T++Y TG++ E+E+Y AETFL FL Sbjct: 1 MIRDYQAIQKTWFVKGKQRIIPTFGKHQGLKLIGTLNYETGEVFCIEEERYDAETFLRFL 60 Query: 75 KKVIDEYPTGKIVMVLDNARIHHAELLTPFLTEMKERLELVFLPPYSPQFNAVEGLWKWL 134 + V++ YPTGKIVM+LDNARIHHA+L+ PFL E ++RLELVFLPPYSPQ N +EGLWKWL Sbjct: 61 QLVLERYPTGKIVMILDNARIHHAKLIQPFLKEHEDRLELVFLPPYSPQLNLIEGLWKWL 120 Query: 135 KSDVINNVFYHTVTEIRTNVQQFMEEIMKVPLTIIDRLCVRF 176 KSDVI NVFY +V EIR NVQ F++ I + P IDRLCV+ Sbjct: 121 KSDVIYNVFYSSVQEIRKNVQAFIQRINQKPEQTIDRLCVQL 162 >B7GF72.1 B7GF72_ANOFW Transposase Length = 162 Score = 236 bits (602), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 110/162 (67%), Positives = 134/162 (82%) Query: 15 MIRDYQAIQKTWFLRGKQRIIPTTGKHRGVKLLATVDYGTGKIVWQEDEQYTAETFLSFL 74 MIRDYQAIQKTWF++GKQRIIPT GKH+G+KL+ T++Y TG++ E+E+Y AETFL FL Sbjct: 1 MIRDYQAIQKTWFVKGKQRIIPTFGKHQGLKLIGTLNYETGEVFCIEEERYDAETFLRFL 60 Query: 75 KKVIDEYPTGKIVMVLDNARIHHAELLTPFLTEMKERLELVFLPPYSPQFNAVEGLWKWL 134 + V++ YPTGKIVM+LDNARIHHA+L+ PFL E ++RLELVFLPPYSPQ N +EGLWKWL Sbjct: 61 QLVLERYPTGKIVMILDNARIHHAKLIQPFLKEHEDRLELVFLPPYSPQLNLIEGLWKWL 120 Query: 135 KSDVINNVFYHTVTEIRTNVQQFMEEIMKVPLTIIDRLCVRF 176 KSDVI NVFY V EIR NVQ F++ I + P IDRLCV+ Sbjct: 121 KSDVIYNVFYSGVQEIRKNVQAFIQRINQKPEQTIDRLCVQL 162 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.139 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,070,019,472 Number of extensions: 42267274 Number of successful extensions: 133393 Number of sequences better than 1.0e-05: 459 Number of HSP's gapped: 133682 Number of HSP's successfully gapped: 459 Length of query: 176 Length of database: 3,060,696,203 Length adjustment: 126 Effective length of query: 50 Effective length of database: 1,873,648,313 Effective search space: 93682415650 Effective search space used: 93682415650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 126 (53.1 bits)