BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0051.p (643 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A5UZB8.1 A5UZB8_ROSS1 Glycoside hydrolase, family 20 488 e-136 A7NGQ6.1 A7NGQ6_ROSCS Glycoside hydrolase family 20 476 e-132 Q67SQ4.1 Q67SQ4_SYMTH N-acetyl-beta-hexosaminidase 465 e-129 A9B0V5.1 A9B0V5_HERA2 Glycoside hydrolase, family 20, catalytic ... 434 e-119 C5HLB1.1 C5HLB1_9BACT Glycoside hydrolase family protein 406 e-111 >A5UZB8.1 A5UZB8_ROSS1 Glycoside hydrolase, family 20 Length = 619 Score = 488 bits (1257), Expect = e-136, Method: Compositional matrix adjust. Identities = 254/552 (46%), Positives = 352/552 (63%), Gaps = 28/552 (5%) Query: 85 QGYEIHVDENGIAVNYGSPEGAYYAAATLKQILQQCGRSLPFVHISDQPDFAARGLMIDI 144 QGY + + + GI + +P G +Y TL QI++Q GR+LP +HISD PDFAARG+MIDI Sbjct: 85 QGYVLTIKDGGIIIEASAPAGIFYGVCTLIQIVEQTGRTLPCLHISDYPDFAARGVMIDI 144 Query: 145 SRNKIPKLETLFRIVDFMADLKLNQLQLYIEGSPFAYESFPQVWELETPITGEEILQLDA 204 SR+K+P +ETLF +VD +A K+NQ+QLY E + FAY + P VW +P+TGEEIL LDA Sbjct: 145 SRDKVPTMETLFMLVDMLAGWKINQVQLYTEHT-FAYRNHPDVWARASPMTGEEILTLDA 203 Query: 205 YCQERYIELVPNQNSFGHMEGWLTRPEFNALAEIPEGFMLPENMYDSDMYPEGLFMHPGT 264 YC+ER+I+LVPNQNSFGHM WL P + ALAE + F P G+ P + Sbjct: 204 YCKERHIDLVPNQNSFGHMHRWLIHPRYAALAETHDAFQAPW----------GVMQGPFS 253 Query: 265 FHTENPEVLQLLATMYDDLLPYFTSKYFNVGCDETYEXXXXXXXXXXXXXXXXQLYLSFL 324 ++P L+L+ ++YD+LLP+F+S+ FNVGCDET + ++YL FL Sbjct: 254 LAPDDPGSLELVRSLYDELLPHFSSRLFNVGCDETVDLGQGRSKDICAQRGVGRVYLDFL 313 Query: 325 QKIHGLVLERGKTMQFWGDIIIQHPELIPRLPKDIVAMEWGYSAAHPFEADTLKFREAGI 384 K++ V + G+TM FWGDI+ HPELI LP+D++A+EWGY A HPF+ + ++ EAGI Sbjct: 314 LKLYDAVKQHGRTMMFWGDIVNNHPELIGELPRDVIALEWGYEADHPFDRNCARYAEAGI 373 Query: 385 PFYVCPGTSSWNSLTGRSDNMMANLRSAAIHGKNNGAIGYLITDWGDFGHWQHLPISYAG 444 PFYVCPGTSSW S+ GR+DN + NL +AA HG +GAIGYLITDWGD GHWQ LPIS+ G Sbjct: 374 PFYVCPGTSSWQSIAGRTDNALGNLHNAAEHGLKHGAIGYLITDWGDMGHWQALPISFPG 433 Query: 445 FAYGAAVAWNVENNLEADVAAYLNTSVFHDRAGKIGQLLLDLGNYYKLESGIVRPNDTEM 504 FA GAA AW N +V A ++ F D G +GQ+ DLGN Y+ G PN + + Sbjct: 434 FAVGAAFAWAYAANRTINVPAAVSRHAFTDPTGAMGQVAYDLGNVYR-AVGYEPPNSSVL 492 Query: 505 SMLLRTPLQQVQLVGKLTVEHFEQLEQYLAEIEARLDQP----ELECADAGLVVAELRNG 560 +L+ P + + L ++ L I+A + QP + ADA L++ E N Sbjct: 493 FWVLQAPDTDARNLPPL------DFDRALDAIDAAI-QPIATERMTRADAPLILQEFDNT 545 Query: 561 IHFVKHAVQLARIKKQLADGPGTL-QPELIHRQIKDLDILLHHYRQLWLQRNRPGGLEQS 619 + ++HA +L ++ Q DGPG L + L++ +++ ++ Y +LWL RNR GGL S Sbjct: 546 VRLLRHACRLGQLLVQ-PDGPGALPRRRLLNNDMRE---IIREYERLWLARNRLGGLSDS 601 Query: 620 VSKLVRLRAEYT 631 V++L R+RA YT Sbjct: 602 VARLERVRARYT 613 >A7NGQ6.1 A7NGQ6_ROSCS Glycoside hydrolase family 20 Length = 619 Score = 476 bits (1225), Expect = e-132, Method: Compositional matrix adjust. Identities = 250/552 (45%), Positives = 347/552 (62%), Gaps = 28/552 (5%) Query: 85 QGYEIHVDENGIAVNYGSPEGAYYAAATLKQILQQCGRSLPFVHISDQPDFAARGLMIDI 144 QGY + + + GI + P G +Y TL QI++Q GRSLP +HI+D PDFAARG+MIDI Sbjct: 85 QGYALTIKDGGIIIEAPEPAGIFYGVCTLIQIIEQSGRSLPCLHINDHPDFAARGVMIDI 144 Query: 145 SRNKIPKLETLFRIVDFMADLKLNQLQLYIEGSPFAYESFPQVWELETPITGEEILQLDA 204 SR+K+P +ETL + D +A K+NQ+QLY E + FAY + P VW +P+TGEE+L LDA Sbjct: 145 SRDKVPTMETLLMLTDMLAGWKINQVQLYTEHT-FAYRNHPDVWARASPLTGEEVLALDA 203 Query: 205 YCQERYIELVPNQNSFGHMEGWLTRPEFNALAEIPEGFMLPENMYDSDMYPEGLFMHPGT 264 YC+ER+IELVPNQNSFGHM WL P + ALAEI F P G+ P + Sbjct: 204 YCKERFIELVPNQNSFGHMHRWLIHPRYAALAEIHGEFQAPW----------GVMRGPFS 253 Query: 265 FHTENPEVLQLLATMYDDLLPYFTSKYFNVGCDETYEXXXXXXXXXXXXXXXXQLYLSFL 324 E+P+ L+L+ ++YD+LLP+F+S+ FNVGCDET + ++YL FL Sbjct: 254 LAPEDPDSLKLVRSLYDELLPHFSSRLFNVGCDETVDLGQGRSRDACAQRGVGRVYLDFL 313 Query: 325 QKIHGLVLERGKTMQFWGDIIIQHPELIPRLPKDIVAMEWGYSAAHPFEADTLKFREAGI 384 K++ V + G+TM FWGDI+ HPELI LP+D++A+EWGY A HPF+ ++ AGI Sbjct: 314 LKLYDAVKQHGRTMMFWGDIVNNHPELIDELPRDLIALEWGYEADHPFDRHCARYAAAGI 373 Query: 385 PFYVCPGTSSWNSLTGRSDNMMANLRSAAIHGKNNGAIGYLITDWGDFGHWQHLPISYAG 444 PFYVCPGTSSW S+ GR+DN + NLR+AA +G +GAIGYLITDWGD GHWQ LPIS+ G Sbjct: 374 PFYVCPGTSSWQSIAGRTDNTLGNLRNAAENGLKHGAIGYLITDWGDMGHWQVLPISFLG 433 Query: 445 FAYGAAVAWNVENNLEADVAAYLNTSVFHDRAGKIGQLLLDLGNYYKLESGIVRPNDTEM 504 FA GAA AW N + +V A ++ F D G +GQ+ DLGN Y+ G PN + + Sbjct: 434 FAVGAAFAWAYAANRDMNVPAAVSRHAFTDPTGAMGQVAYDLGNVYR-AVGYEPPNSSVL 492 Query: 505 SMLLRTPLQQVQLVGKLTVEHFEQLEQYLAEIEA----RLDQPELECADAGLVVAELRNG 560 +L+ P + + L + L+ A I+ R+ +P DA L++ E N Sbjct: 493 FWVLQAPDSDARDLPPLDFD--RALDAINAAIQPIAVERMIRP-----DAPLILQEFDNT 545 Query: 561 IHFVKHAVQLARIKKQLADGPGTL-QPELIHRQIKDLDILLHHYRQLWLQRNRPGGLEQS 619 + ++HA +L ++ Q DG G L + L++ +++ ++ Y +LWL RNR GGL S Sbjct: 546 VRLLRHACRLGQLLTQ-PDGAGALPRRRLLNDDMRE---IIREYERLWLARNRLGGLSDS 601 Query: 620 VSKLVRLRAEYT 631 V++L R+RA Y Sbjct: 602 VARLERVRARYN 613 >Q67SQ4.1 Q67SQ4_SYMTH N-acetyl-beta-hexosaminidase Length = 645 Score = 465 bits (1196), Expect = e-129, Method: Compositional matrix adjust. Identities = 247/537 (45%), Positives = 331/537 (61%), Gaps = 20/537 (3%) Query: 85 QGYEIHVDENGIAVNYGSPEGAYYAAATLKQILQQCGRSLPFVHISDQPDFAARGLMIDI 144 QGY + V +GI + +P GA Y ATLKQ+L GR +P V I+D PDF RG+M+DI Sbjct: 109 QGYRLTVSPDGITIGARAPAGAAYGLATLKQLLSG-GRRVPAVTIADWPDFRRRGVMLDI 167 Query: 145 SRNKIPKLETLFRIVDFMADLKLNQLQLYIEGSPFAYESFPQVWELETPITGEEILQLDA 204 SR K+P +E+L+R VD +ADLK+N+ QLY E + FAY +VW+ +P+TGEEI+ LD Sbjct: 168 SRGKVPTMESLYRFVDLLADLKINEFQLYTEHT-FAYREHREVWQAYSPMTGEEIMLLDR 226 Query: 205 YCQERYIELVPNQNSFGHMEGWLTRPEFNALAEIPEGFMLPENMYDSDMYPEGLFMHPGT 264 YC+ER+I+LVPNQNSFGHM WL + +AE P+GF LP G P + Sbjct: 227 YCRERFIDLVPNQNSFGHMTPWLIHSRYKHMAEAPDGFELPWG---------GRRNEPFS 277 Query: 265 FHTENPEVLQLLATMYDDLLPYFTSKYFNVGCDETYEXXXXXXXXXXXXXXXXQLYLSFL 324 P V+ LA +YD+LLP+FTS+YFNVGCDET++ ++YL L Sbjct: 278 LSPAEPSVISFLAGLYDELLPHFTSQYFNVGCDETWDVGQGRSARAVAERGEHEVYLEQL 337 Query: 325 QKIHGLVLERGKTMQFWGDIIIQHPELIPRLPKDIVAMEWGYSAAHPFEADTLKFREAGI 384 KIH LV ER +TMQFWGDIII++PE + RLPKD++A+EWGY A HPF+ + AG+ Sbjct: 338 LKIHRLVAERRRTMQFWGDIIIKYPEAVARLPKDVIALEWGYEADHPFDEHCAVYAHAGV 397 Query: 385 PFYVCPGTSSWNSLTGRSDNMMANLRSAAIHGKNNGAIGYLITDWGDFGHWQHLPISYAG 444 PFYVCPGTS+W SL GR++N + N+RSAA G +GA G+L T WGD GH + P++Y Sbjct: 398 PFYVCPGTSTWMSLVGRTENALQNMRSAAESGLKHGAQGFLNTIWGDLGHQDYEPVAYLP 457 Query: 445 FAYGAAVAWNVENNLEADVAAYLNTSVFHDRAGKIGQLLLDLGNYYKLESGIVRPNDTEM 504 A+GAAV+W +E N EADV +L+ VF D AG+IG +L DLGN Y L +G N T + Sbjct: 458 VAFGAAVSWALEANREADVRPFLDRLVFEDGAGQIGSVLYDLGNTY-LAAGPRIHNGTAL 516 Query: 505 SMLLRTPLQQVQLVGKLTVE-HFEQLEQYLAEIEARLDQPELECADAGLVVAELRNGIHF 563 L + + KL E F + + A LD +L CA LV+ ELRN + Sbjct: 517 GRLFHSARTWREW--KLLPEIDFAAQAEAVRRAAAGLDLADLRCARGPLVMRELRNAVRL 574 Query: 564 VKHAVQLARIKKQLADG--PGTLQPELIHRQIKDLDILLHHYRQLWLQRNRPGGLEQ 618 + L + + + G P Q E + R+ L+ + +R LWLQRNRPGGLE+ Sbjct: 575 LLFLCDLGQTLQGVLRGSLPSAEQREALGRE---LEAIACEHRDLWLQRNRPGGLEE 628 >A9B0V5.1 A9B0V5_HERA2 Glycoside hydrolase, family 20, catalytic core Length = 599 Score = 434 bits (1115), Expect = e-119, Method: Compositional matrix adjust. Identities = 228/554 (41%), Positives = 321/554 (57%), Gaps = 22/554 (3%) Query: 79 SPLLPAQGYEIHVDENGIAVNYGSPEGAYYAAATLKQILQQCGRSLPFVHISDQPDFAAR 138 S ++ A GY + + +G+ + + G +Y TL+QI QQ LP++ I D PDF R Sbjct: 60 SSIVHADGYRLQIAADGVQIVAQTAAGLFYGLQTLRQIQQQSAEQLPYLLIDDAPDFVVR 119 Query: 139 GLMIDISRNKIPKLETLFRIVDFMADLKLNQLQLYIEGSPFAYESFPQVWELETPITGEE 198 G M+DISR+K+P + TL+ ++D +A K+NQ+QLYIE + FAY++ P VW +P+T EE Sbjct: 120 GFMLDISRDKVPTMATLYALIDELASWKINQIQLYIEHT-FAYQNHPTVWADASPLTAEE 178 Query: 199 ILQLDAYCQERYIELVPNQNSFGHMEGWLTRPEFNALAEIPEGFMLPENMYDSDMYPEGL 258 + LD +C ER+IELVPNQN FGHM WLT+P + LAE P+G + D D G Sbjct: 179 VRALDDFCLERFIELVPNQNCFGHMRRWLTKPAYRDLAECPDGC----DTGDPDW---GY 231 Query: 259 FMHPGTFHTENPEVLQLLATMYDDLLPYFTSKYFNVGCDETYEXXXXXXXXXXXXXXXXQ 318 F P T E+P ++L+ +M D+LLP F ++ NVGCDET E + Sbjct: 232 FEEPFTLAPEHPGSIELVRSMLDELLPNFRTRTLNVGCDETVELGLGASAAAVAERGKGR 291 Query: 319 LYLSFLQKIHGLVLERGKTMQFWGDIIIQHPELIPRLPKDIVAMEWGYSAAHPFEADTLK 378 +YL FLQK++ RG MQFW DII+ +PEL+ LP+D + WGY + HPFE Sbjct: 292 VYLEFLQKLYHEAHSRGYVMQFWSDIILHYPELVSELPRDAEVLIWGYESHHPFEEQAAT 351 Query: 379 FREAGIPFYVCPGTSSWNSLTGRSDNMMANLRSAAIHGKNNGAIGYLITDWGDFGHWQHL 438 +AG+PFYVCPGTSSWN++ GR+ N + N+ SAA HG N+GA G+L+TDWGD GHWQ L Sbjct: 352 IAKAGLPFYVCPGTSSWNTVAGRTTNALENIASAAKHGLNHGAKGFLMTDWGDNGHWQPL 411 Query: 439 PISYAGFAYGAAVAWNVENNLEADVAAYLNTSVFHDRAGKIGQLLLDLGNYYKLESGIVR 498 SY G A GAA AWN L DV A L+T VF D +G L+ LG+ Y+ ++ Sbjct: 412 VTSYLGLAIGAARAWNALAEL--DVVALLDTVVFADSNKVLGNLVYQLGDVYRAVPPLLH 469 Query: 499 PNDTEMSMLLRTPLQQVQLVGKLTVEHFEQLEQYLAEIEARLDQPELECADAGLVVAELR 558 + +L P +L L ++ ++ L ++ L + + AD L EL Sbjct: 470 NTSSLFRILQANPAAIAEL--NLDRKNLHNADEILLQLRNDLQTIKPQRADGELCQVELA 527 Query: 559 NGIHFVKHAVQ--LARIKKQLADGPGTLQPELIHRQIKDLDILLHHYRQLWLQRNRPGGL 616 I ++HAVQ L + +Q ++ LQPE +D L+ ++Q+WL RNRPGGL Sbjct: 528 WAIDLLRHAVQRGLWVLDQQPSETASKLQPE--------IDALIERFQQVWLSRNRPGGL 579 Query: 617 EQSVSKLVRLRAEY 630 + S+ LR Y Sbjct: 580 QDSLKHFASLRDSY 593 >C5HLB1.1 C5HLB1_9BACT Glycoside hydrolase family protein Length = 638 Score = 406 bits (1043), Expect = e-111, Method: Compositional matrix adjust. Identities = 226/546 (41%), Positives = 313/546 (57%), Gaps = 36/546 (6%) Query: 84 AQGYEIHVDENGIAVNYGSPEGAYYAAATLKQI--LQQCGRSLPFVHISDQPDFAARGLM 141 A+GY I + I V G + A+ TLKQI L + LP + I+D PDF RG M Sbjct: 107 AEGYTISISRRVITVRAHDEAGLFRASQTLKQIGRLARTRGHLPCIDITDWPDFPNRGAM 166 Query: 142 IDISRNKIPKLETLFRIVDFMADLKLNQLQLYIEGSPFAYESFPQVWELETPITGEEILQ 201 +D++R K+P++E+L+ +VD +A+ K NQ+QLY + + FAY +WE +P+T +I Sbjct: 167 LDVTRTKVPEMESLYALVDKLAEWKYNQIQLYFQHA-FAYRGHHPIWENASPMTPVQIRA 225 Query: 202 LDAYCQERYIELVPNQNSFGHMEGWLTRPEFNALAEIPEGFMLPENMYDSDMYPEGLFMH 261 LDAYC++RYIELVPNQ FGHM+ WL P++N LAEI G P ++ +D Sbjct: 226 LDAYCRDRYIELVPNQECFGHMQNWLWHPDYNHLAEI-AGAKSPADLCPTD--------- 275 Query: 262 PGTFHTENPEVLQLLATMYDDLLPYFTSKYFNVGCDETYEXXXXXXXXXXXXXXXXQLYL 321 P ++ L +Y DLLP FTS+ FNVGCDE ++YL Sbjct: 276 --------PASIEFLDGLYADLLPNFTSRQFNVGCDEVGTLGLGRSKAVVEEKGQGRVYL 327 Query: 322 SFLQKIHGLVLERGKTMQFWGDIIIQHPELIPRLPKDIVAMEWGYSAAHPFEADTLKFRE 381 FL +I+ V +RG MQFW DI++QHPELIP LPKD++A+EWGY HPF+ + Sbjct: 328 DFLLQIYERVKKRGFRMQFWADIVLQHPELIPELPKDVIALEWGYGEHHPFDERCRLLAQ 387 Query: 382 AGIPFYVCPGTSSWNSLTGRSDNMMANLRSAAIHGKNNGAIGYLITDWGDFGHWQHLPIS 441 +GI FYV PGTSSW SL G DN +ANL+SAA +G +GAIGYL+TDWGD GHWQ +S Sbjct: 388 SGISFYVAPGTSSW-SLIGMHDNAVANLKSAAENGLKHGAIGYLVTDWGDSGHWQFRSVS 446 Query: 442 YAGFAYGAAVAWNVENNLEADVAAYLNTSVFHDRAGKIGQLLLDLGNYYKLES-GIVRPN 500 Y G YGAAV+W E N++ D+ L+ VF D AG +GQL+ D+G Y+ + RP Sbjct: 447 YPGLTYGAAVSWCYETNVDVDIPRALDAHVFEDAAGVMGQLVYDMGLAYRQNGVSLFRP- 505 Query: 501 DTEMSMLLRTPLQQVQLV-----GKLTVEHFEQLEQYLAEIEARLDQPELECADAGLVVA 555 LLR P ++ + E + Y+ ++ ARL + + C DA V Sbjct: 506 ------LLRKPDASFSVIPPHLRPAASKESLLKTIAYMDDVMARLPETRMACPDADQTVE 559 Query: 556 ELRNGIHFVKHAVQLARIKKQLADGPGTLQPELIHRQI-KDLDILLHHYRQLWLQRNRPG 614 E + + A +L + + P + P I R++ +L+ L+ YRQLWL RNR G Sbjct: 560 EWKLAAALARLACRLGIARDEHDGAPTSALPADIRRELATELEPLIPAYRQLWLARNRSG 619 Query: 615 GLEQSV 620 GL+QS Sbjct: 620 GLKQSA 625 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,778,548,144 Number of extensions: 158108876 Number of successful extensions: 361528 Number of sequences better than 1.0e-05: 497 Number of HSP's gapped: 361221 Number of HSP's successfully gapped: 499 Length of query: 643 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 501 Effective length of database: 1,722,912,073 Effective search space: 863178948573 Effective search space used: 863178948573 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)