BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0048.p (548 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6TKJ7.1 A6TKJ7_ALKMQ Integral membrane sensor signal transducti... 205 9e-51 C0GEE5.1 C0GEE5_9FIRM Multi-sensor signal transduction histidine... 190 4e-46 B8G2Y3.1 B8G2Y3_CHLAD GAF sensor signal transduction histidine k... 102 8e-20 A6CLJ0.1 A6CLJ0_9BACI Two-component sensor histidine kinase 99 1e-18 B9LCJ4.1 B9LCJ4_CHLSY GAF sensor signal transduction histidine k... 96 7e-18 >A6TKJ7.1 A6TKJ7_ALKMQ Integral membrane sensor signal transduction histidine kinase Length = 570 Score = 205 bits (522), Expect = 9e-51, Method: Compositional matrix adjust. Identities = 145/551 (26%), Positives = 267/551 (48%), Gaps = 21/551 (3%) Query: 10 KVITIYRNISLSLTSLMYLLDHTGPSVFYKSLLIMVLFLFAQVFTIYYRRLRKWPRVLML 69 K+I +YR +SL +TS YLL + S K ++ + + + + + Y + + +M+ Sbjct: 20 KIIFLYRYVSLIVTSSFYLLADSTHSFENKIFIVACISISSVIISYLYIKNQNCTDKIMI 79 Query: 70 AVSIEMAGLIVLTLFTGGYDSSFKLYVLNPVLIAAGSLSIYFSWSLLFSYFGIISVFCYF 129 + IE G + + +GG +S + Y LN ++IA+ +L + W L Y + + Sbjct: 80 LILIETIGNSFILIPSGGLNSPYVWYSLNTIVIASVALHKKYCWINLVVYLFSSTYLSHL 139 Query: 130 FLNSSGKTFAEAVLDNGNLFLVMVLTVIVMQMVNR--------------IKRQREAANAR 175 L +T + + + NL L +L ++Q++ + R+ AN + Sbjct: 140 ILQKE-QTLMQIISEESNLILSFILITGIIQLLTKYAKRVQDEGLKLTETNRKLVLANKK 198 Query: 176 TNETMEHIKSLYHIVET-SSQHDFMNIGQVITDYVVKLTKLDKALFWFAQKSGEPAPQSR 234 E++ +I LY + ++Q ++ +++ DY K+ K D +F ++ G+ Sbjct: 199 NKESLGYIMELYEATQLFTTQETPSDLIELMLDYSQKVMKTDTVIFIHPRREGKKVV-IE 257 Query: 235 QTGWHQEEERFLFAELEKHEHEWRLQREPVFKSLPGLGDFLLMPVRMSTRFVGMIGVKLE 294 ++ E + E+ H ++ E + + ++ + ++ + + G++G+ Sbjct: 258 SNNLAEDLEEVICCEISSIWHN-TVESEIPIEMIIKDQKYVFIAIKYNYKVFGILGITRT 316 Query: 295 ASEGLEGRRWYIQQLMFLSELSAIILERHELGVIENRLIITNEQNRIADEMHDSVSQSLF 354 +++ +QL FL+ LS+I LE+ EL + L+I EQNRIA+E+HD + Q LF Sbjct: 317 STKMQNNYEEITEQLRFLASLSSITLEKFELEKVTEGLLINEEQNRIANEIHDGILQRLF 376 Query: 355 GIVYATHSLKQTWRKMXXXXXXXXXXXXHDSATKVAKELRITIYSLSSKKSGGPTWLGMV 414 S+ + R+M + V K+LR T+Y LS KK G ++ + Sbjct: 377 STSCGVFSIVKRVREMNTDEIEEELNIIRRAVNNVMKDLRSTVYGLSWKKDGANNFISDI 436 Query: 415 RSHLKSLSRLNDVEIELKITGDDFSLPYPYHKALFRIISEATGNAIRHGAASRVDVELSL 474 S++ + LN+++I+ +TGDD L KA++RII E GNA+RHG A ++V + Sbjct: 437 ESYINEIKALNNIDIKFDLTGDDELLSIAQKKAMYRIICEGIGNAVRHGNAQNIEVSIIS 496 Query: 475 KPKWIRLSIVDDGVGFDTDLLWTESEDNTGGLGMKNMQYLAQSLGGDFQLSSSENAGTRI 534 + + + L I DDG GF+ + + GLG+KN+ +L SL G + S GTRI Sbjct: 497 RRQGMSLIINDDGKGFNMRGI---DKSERFGLGVKNIYHLTHSLNGTIKFESQIGKGTRI 553 Query: 535 LISIPVGVAEL 545 I IP + E+ Sbjct: 554 NIVIPNVIQEM 564 >C0GEE5.1 C0GEE5_9FIRM Multi-sensor signal transduction histidine kinase Length = 747 Score = 190 bits (482), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 120/379 (31%), Positives = 193/379 (50%), Gaps = 14/379 (3%) Query: 175 RTNETMEHIKSLYHIVET-SSQHDFMNIGQVITDYVVKLTKLDKALFWFAQKSGEPAPQS 233 RT +TM+ + +LY VET SS+ D + + Y LT K +FW + S + Sbjct: 366 RTEQTMQRMTALYEAVETISSRDDIKEVIDLFAAYARALTGNKKVIFWLDKTSVKEDSSL 425 Query: 234 RQTGWHQEEERFLFAELEKHEH---EW-RLQREP------VFKSL-PGLGDFLLMPVRMS 282 + + E+ + E +E W R+Q+ P V+ S + L MPV+ Sbjct: 426 NNRIYTVKGEKDVLPESYWYEPLIAAWSRIQKTPHSVEHLVYDSQGKPVARLLCMPVKSR 485 Query: 283 TRFVGMIGVKLEASEGLEGRRWYIQQLMFLSELSAIILERHELGVIENRLIITNEQNRIA 342 + G++ L+ + +Q L F++ L+A+ +ER+ + +++++ EQNRIA Sbjct: 486 SHCFGLLAA-LQPKDAFNTEE-VMQTLSFMANLAAVAIERNIAELFSDKMLLLEEQNRIA 543 Query: 343 DEMHDSVSQSLFGIVYATHSLKQTWRKMXXXXXXXXXXXXHDSATKVAKELRITIYSLSS 402 +E+HDSVSQ+LF +VY ++ + + D K AKELR+ IY LS Sbjct: 544 NEIHDSVSQNLFSVVYGAEAIIRQGDTLSPNEKKQILQAIRDVTAKTAKELRLLIYRLSP 603 Query: 403 KKSGGPTWLGMVRSHLKSLSRLNDVEIELKITGDDFSLPYPYHKALFRIISEATGNAIRH 462 + G T++ + L L+ LN ++I+ I+G + L +A +RI+ EAT NAIRH Sbjct: 604 RHRGDHTFVSELEKQLNGLAELNQIDIDFNISGKEEYLNAAIRRAFYRIVKEATNNAIRH 663 Query: 463 GAASRVDVELSLKPKWIRLSIVDDGVGFDTDLLWTESEDNTGGLGMKNMQYLAQSLGGDF 522 G + + V+L + P +L I DDG G D DL T +E + LG+ NM LA+SL G Sbjct: 664 GKSKNIKVDLLMDPFESQLFIQDDGKGCDIDLYSTNAESSARKLGVVNMTELARSLQGHL 723 Query: 523 QLSSSENAGTRILISIPVG 541 + S GT+I +S+P Sbjct: 724 NIESKVGEGTKISVSVPTS 742 Score = 60.1 bits (144), Expect = 5e-07, Method: Compositional matrix adjust. Identities = 52/184 (28%), Positives = 83/184 (45%), Gaps = 5/184 (2%) Query: 14 IYRNISLSLTSLMYLLDHTGPSVFYKSLLIMVLFLFAQVFTIYYRRLRKWPRVLMLAVSI 73 ++R ISL LTS++++L +F K ++ L + + +F Y R K L + V Sbjct: 35 LFRAISLLLTSIVFMLAPGQRLLFDKLGFLLFLVISSFIFVYLYNRAIKRSDNLAVYVLA 94 Query: 74 EMAGLIVLTLFTGGYDSSFKLYVLNPVLIAAGSLSIYFSWSLLFSY--FGIISVFCYFFL 131 E L ++ TGG DS F Y LNPV+IA+ L + W+L+ Y F I+ + Sbjct: 95 ESVILAIIVALTGGLDSPFIWYALNPVMIASAYLPFFMPWTLMSVYLLFSILEDLYFEGA 154 Query: 132 NSSGKTFAEAVLDNGNLFLVMVLTVIVMQMVNRIKRQREAANARTNETMEHIKSLYHIVE 191 N S F L L + L + Q+ + I R + E E + Y+ +E Sbjct: 155 NFSLLGFFSNYL---GLISALFLMALAFQIFSGIYRVLAVQSQALEEQQEKLTEAYNSLE 211 Query: 192 TSSQ 195 + Q Sbjct: 212 KNHQ 215 >B8G2Y3.1 B8G2Y3_CHLAD GAF sensor signal transduction histidine kinase Length = 725 Score = 102 bits (255), Expect = 8e-20, Method: Compositional matrix adjust. Identities = 71/266 (26%), Positives = 123/266 (46%), Gaps = 7/266 (2%) Query: 275 LLMPVRMSTRFVGMIGVKLEASEGLEGRRWYIQQLMFLSELSAIILERHELGVIENRLII 334 L +P+ + + +G++ ++ GL + L ++++ A +E L + Sbjct: 459 LCVPLIANGKTLGVLNIESPQPAGLTAEDAAV--LETVADMLAGAVENGRLYQRAQQAAA 516 Query: 335 TNEQNRIADEMHDSVSQSLFGIVYATHSLKQTWRKMXXXXXXXXXXXXHDSATKVAKELR 394 E+NR+A E+HDSVSQ LF + + + + + H++AT E+R Sbjct: 517 LEERNRLARELHDSVSQQLFSMTLTAQAARSQFER-NPARVPVLLERLHETATAALAEMR 575 Query: 395 ITIYSLSSKKSGGPTWLGMVRSHLKSLSRLNDVEIELKITGDDFSLPYPYHKALFRIISE 454 I+ L + ++ H + L+ + IEL + GD+ + + LFRI+ E Sbjct: 576 ALIFQLRPPALRDQGLVAAIQQHAQHLAHREGLRIELNVIGDERHV-RGIEQPLFRIVQE 634 Query: 455 ATGNAIRHGAASRVDVELSLKPKWIRLSIVDDGVGFDTDLLWTESEDNTGGLGMKNMQYL 514 A N ++H AA V ++L L RL + DDG GFD + + G LGM+ Sbjct: 635 ALNNIVKHAAARNVRIQLELTVDQARLCVADDGQGFDPKAYPSGEGRHLGLLGMRER--- 691 Query: 515 AQSLGGDFQLSSSENAGTRILISIPV 540 A LGG F + S +GT I + +P+ Sbjct: 692 AAELGGSFTVRSHPGSGTEIEVIVPL 717 >A6CLJ0.1 A6CLJ0_9BACI Two-component sensor histidine kinase Length = 348 Score = 99.0 bits (245), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 61/208 (29%), Positives = 103/208 (49%), Gaps = 8/208 (3%) Query: 333 IITNEQNRIADEMHDSVSQSLFGIVYATHSLKQTWRKMXXXXXXXXXXXXHDSATKVAKE 392 I++ E+NR+A E+HDSVSQ LF ++ ++ + E Sbjct: 141 IVSQERNRLARELHDSVSQQLFAASMMMSAITESKSGTSTPTESKQLKMVEGMIHQSQLE 200 Query: 393 LRITIYSLSSKKSGGPTWLGMVRSHLKSLSRLNDVEIELKITGDDFSLPYPYHKALFRII 452 +R + L G T ++ L L+ +E+E KI ++ SL LFRI+ Sbjct: 201 MRALLLHLRPAALKGKTLHEGIKELLVELNNKVPMEVEWKI--EEISLDKGIEDHLFRIL 258 Query: 453 SEATGNAIRHGAASRVDVELSLKPKWIRLSIVDDGVGFDTDLLWTESEDNTGGLGMKNMQ 512 E+ N +RH A+ ++V L + + L ++DDG+GFD++L + TG G++NMQ Sbjct: 259 QESVSNTLRHSKANSLEVLLIKRDSLVILRVMDDGIGFDSEL------EKTGSYGLQNMQ 312 Query: 513 YLAQSLGGDFQLSSSENAGTRILISIPV 540 A +GG ++ S N GTR+ + +P+ Sbjct: 313 ERALEIGGSLKIVSVPNKGTRLEVKVPM 340 >B9LCJ4.1 B9LCJ4_CHLSY GAF sensor signal transduction histidine kinase Length = 731 Score = 96.3 bits (238), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 57/205 (27%), Positives = 94/205 (45%), Gaps = 5/205 (2%) Query: 336 NEQNRIADEMHDSVSQSLFGIVYATHSLKQTWRKMXXXXXXXXXXXXHDSATKVAKELRI 395 E+NR+A E+HDSVSQ LF + + + + + H++AT E+R Sbjct: 521 EERNRLARELHDSVSQQLFSMTLTAQAARSQFER-NPARVPALLDRLHETATAALAEMRA 579 Query: 396 TIYSLSSKKSGGPTWLGMVRSHLKSLSRLNDVEIELKITGDDFSLPYPYHKALFRIISEA 455 I+ L + ++ H + L+ + IEL + GD+ + LFRI+ EA Sbjct: 580 LIFQLRPPALRDQGLVAAIQQHAQHLAHREGLRIELNVIGDERHA-RGIEQPLFRIVQEA 638 Query: 456 TGNAIRHGAASRVDVELSLKPKWIRLSIVDDGVGFDTDLLWTESEDNTGGLGMKNMQYLA 515 N ++H AA V + L + + ++DDG GFD LG+ +M+ A Sbjct: 639 LNNIVKHAAARNVQIMLEFNADQVAIRVIDDGKGFDP---AARPSGEGRHLGLLSMRERA 695 Query: 516 QSLGGDFQLSSSENAGTRILISIPV 540 LGG F + S AG + + +P+ Sbjct: 696 AELGGSFNVHSRPGAGAEVEVVVPL 720 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,947,983,801 Number of extensions: 115684851 Number of successful extensions: 375756 Number of sequences better than 1.0e-05: 1649 Number of HSP's gapped: 375418 Number of HSP's successfully gapped: 1650 Length of query: 548 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 407 Effective length of database: 1,732,333,088 Effective search space: 705059566816 Effective search space used: 705059566816 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)