BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0033.p (446 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D9N7.1 B1D9N7_9BACL Nucleotide sugar dehydrogenase EC=1.1.1.22 546 e-153 B1D9I6.1 B1D9I6_9BACL Nucleotide sugar dehydrogenase EC=1.1.1.22 468 e-130 B2A1P9.1 B2A1P9_NATTJ Nucleotide sugar dehydrogenase EC=1.1.1.22 466 e-129 O32271.1 TUAD_BACSU UDP-glucose 6-dehydrogenase tuaD EC=1.1.1.22 453 e-125 C3ED88.1 C3ED88_BACTU NDP-sugar dehydrogenase (Teichuronic acid ... 452 e-125 >B1D9N7.1 B1D9N7_9BACL Nucleotide sugar dehydrogenase EC=1.1.1.22 Length = 445 Score = 546 bits (1408), Expect = e-153, Method: Compositional matrix adjust. Identities = 267/432 (61%), Positives = 339/432 (78%) Query: 1 MKLAVIGTGYVGLVSGVCFTLNGNHVICVDKDEEKIKKLSQMESPIYEPGIEALIEMNLR 60 MKLAVIGTGYVGLVSGVCF+ GN V+CVD KI+ L+ E PI+EPG++ LI N Sbjct: 1 MKLAVIGTGYVGLVSGVCFSELGNEVVCVDNATSKIELLNNGEVPIHEPGLKELIASNTA 60 Query: 61 EGRLSFSSDLHESVRRSDIVILAVGTPSLPGGEADLRYIEGAATEIAQAMEGYKIIMTKS 120 GRLSF++DL +V S+I+I+AVGTPSLP G+A+L YIE AA EI +A+ GYK+++ KS Sbjct: 61 AGRLSFTTDLAAAVNDSEIIIIAVGTPSLPDGKANLAYIEQAAAEIGRAINGYKVVVVKS 120 Query: 121 TVPVGTNEKIRKIIASHTNHPFDIVSAPEFLREGSAIQDTLHPDRIVIGLDNPELEPAMR 180 TVPVGTNEK+ II + FD S PEFLREG+A++DTL+PDRI+IG ++ + Sbjct: 121 TVPVGTNEKVAAIIRDYATESFDFASVPEFLREGTAVKDTLNPDRIIIGTESGRAAEMLT 180 Query: 181 QLHQGFTENVFVTDIRSAEMIKYASNAFLATKISFINEIANICEKVGADVTEVAEGMGMD 240 LH+ T+ + +TD+RS+EMIKYASNAFLATKISFINEIANICEKVGADV EVA+GMGMD Sbjct: 181 ALHRPLTDQITITDVRSSEMIKYASNAFLATKISFINEIANICEKVGADVEEVAKGMGMD 240 Query: 241 RRIGSTFLQAGIGYGGSCFPKDTNALIQIAGNVDYEFKLLKSVVEVNKDQRFMIISKLHE 300 RRIGS+FL+AGIGYGGSCFPKDT ALIQIAG +DY+FKLLKSVVEVN+DQRF +I KL E Sbjct: 241 RRIGSSFLKAGIGYGGSCFPKDTQALIQIAGQMDYDFKLLKSVVEVNRDQRFNVIRKLDE 300 Query: 301 SLGNLRGAVIGIWGLAFKPNTDDVREAPAREIVESLVAEGATVKLYDPIAAENFRQQYDH 360 LG++ G IGIWGLAFKP TDDVR+APA EI+E+L+ GA VK YDPIA+ NF + +D Sbjct: 301 LLGDVTGRTIGIWGLAFKPETDDVRDAPAFEIMETLLERGALVKAYDPIASSNFEKAFDR 360 Query: 361 PQLRWCGLPEEAAEGSDAVCLLTDWSVFKDIDLHKLAESMRRQVLIDGRNVFSKEQIEGT 420 ++ +C +EAAEG+DA+C+LT+W F +I L + + ++ LIDGRN++ +E +E T Sbjct: 361 SEVEYCTSAQEAAEGADALCILTEWKEFINIPLSDIQKWLKTPNLIDGRNIYREEDLEET 420 Query: 421 GFEYHSVGRPQM 432 F Y+S+GRP+M Sbjct: 421 PFAYYSIGRPKM 432 >B1D9I6.1 B1D9I6_9BACL Nucleotide sugar dehydrogenase EC=1.1.1.22 Length = 447 Score = 468 bits (1204), Expect = e-130, Method: Compositional matrix adjust. Identities = 233/431 (54%), Positives = 305/431 (70%), Gaps = 4/431 (0%) Query: 2 KLAVIGTGYVGLVSGVCFTLNGNHVICVDKDEEKIKKLSQMESPIYEPGIEALIEMNLRE 61 K+ VIGTGYVGLVSG CF GN VIC D D+ KI L+ PIYEPG+E + N E Sbjct: 3 KITVIGTGYVGLVSGTCFAEVGNRVICCDVDQRKIDLLNSGVIPIYEPGLEEMALRNKSE 62 Query: 62 GRLSFSSDLHESVRRSDIVILAVGTPSLPGGEADLRYIEGAATEIAQAMEGYKIIMTKST 121 GRL F++++ E++ SDIV +AVGTP GEAD+RY+ AA +I + + G+KII+ KST Sbjct: 63 GRLLFTTEVGEAIEASDIVYIAVGTPMSETGEADMRYVMSAAKKIGEHLNGHKIIVNKST 122 Query: 122 VPVGTNEKIRKIIASHTNHP---FDIVSAPEFLREGSAIQDTLHPDRIVIGLDNPELEPA 178 VPVGT E +R+++ ++ +P FD+VS PEFLREG+A+ D ++ DR +IG DN E Sbjct: 123 VPVGTGELVREVVQANKKYPWTSFDVVSNPEFLREGTALSDCMNMDRAIIGSDNAEAAEQ 182 Query: 179 MRQLHQGFTENVFVTDIRSAEMIKYASNAFLATKISFINEIANICEKVGADVTEVAEGMG 238 + QLH F +F TD+ SAEMIKYA+N FLATKISFIN IANICEKVGADV +VA+GMG Sbjct: 183 IAQLHAPFKTRIFKTDLESAEMIKYAANTFLATKISFINAIANICEKVGADVEDVAKGMG 242 Query: 239 MDRRIGSTFLQAGIGYGGSCFPKDTNALIQIAGNVDYEFKLLKSVVEVNKDQRFMIISKL 298 MD RIG FLQAGIGYGGSCFPKDT AL IA Y+F+LLKSV++ N QRF+++ KL Sbjct: 243 MDSRIGEKFLQAGIGYGGSCFPKDTYALAHIADKSGYDFELLKSVIKTNHRQRFVVMDKL 302 Query: 299 HESLGNLRGAVIGIWGLAFKPNTDDVREAPAREIVESLVAEGATVKLYDPIAAENFRQQY 358 E+LG L G I + GLAFKPNTDD+REAP+ I+ L+ +GA V+ +DPIAAE ++Q Sbjct: 303 RETLGGLAGKQIAVLGLAFKPNTDDMREAPSLSIIPELLKQGAVVRAFDPIAAEAAKKQL 362 Query: 359 DHPQLRWCGLPEEAAEGSDAVCLLTDWSVFKDIDLHKLAESMRRQVLIDGRNVFSKEQIE 418 ++ + E AA G DA +LT+W+ +DL K+ + M++ ++IDGRN F + +E Sbjct: 363 P-AEVAYLESAELAAAGCDACVILTEWNEVLTMDLAKVRDRMKQPIMIDGRNSFDPKVME 421 Query: 419 GTGFEYHSVGR 429 GF Y+SVGR Sbjct: 422 QHGFVYYSVGR 432 >B2A1P9.1 B2A1P9_NATTJ Nucleotide sugar dehydrogenase EC=1.1.1.22 Length = 427 Score = 466 bits (1199), Expect = e-129, Method: Compositional matrix adjust. Identities = 229/423 (54%), Positives = 300/423 (70%), Gaps = 1/423 (0%) Query: 2 KLAVIGTGYVGLVSGVCFTLNGNHVICVDKDEEKIKKLSQMESPIYEPGIEALIEMNLRE 61 K++VIGTGYVGL +GVC + GN V CVD DEEKI L +SPIYEPG+E LI N + Sbjct: 3 KISVIGTGYVGLSTGVCLSDMGNQVTCVDIDEEKINTLKNGKSPIYEPGMEDLIHKNTKA 62 Query: 62 GRLSFSSDLHESVRRSDIVILAVGTPSLPGGEADLRYIEGAATEIAQAMEGYKIIMTKST 121 RL F+++L +V+ +DI+ +AVGTPS G ADL +I A+ IA+AM YKII+TKST Sbjct: 63 DRLQFTTNLESAVQNTDIIFIAVGTPSRDDGSADLSFIYEASKTIAKAMNDYKIIVTKST 122 Query: 122 VPVGTNEKIRKIIASHTNHPFDIVSAPEFLREGSAIQDTLHPDRIVIGLDNPELEPAMRQ 181 VPVGTN++I I+ +T+ F +VS+PEFLREGSAI DT++PDRIVIG + + ++ Sbjct: 123 VPVGTNKEIEDSISQNTDQDFSVVSSPEFLREGSAIYDTMNPDRIVIGYRDEQAGQTIKD 182 Query: 182 LHQGFTENVFVTDIRSAEMIKYASNAFLATKISFINEIANICEKVGADVTEVAEGMGMDR 241 L++ F +T S+EMIKYASNAFLATKISFINE+ANICE+VGA+V EVA+GMG+D Sbjct: 183 LYKDFDTEFVMTTTESSEMIKYASNAFLATKISFINEMANICERVGANVEEVAKGMGLDH 242 Query: 242 RIGSTFLQAGIGYGGSCFPKDTNALIQIAGNVDYEFKLLKSVVEVNKDQRFMIISKLHES 301 RI FL AGIGYGGSCFPKDT ALI A VDY K++K+ EVN+ Q+ +++ KL E Sbjct: 243 RISDKFLNAGIGYGGSCFPKDTKALINKAEEVDYNLKIVKAAEEVNEKQKLIVVDKLKEV 302 Query: 302 LGNLRGAVIGIWGLAFKPNTDDVREAPAREIVESLVAEGATVKLYDPIAAENFRQQYDHP 361 LG+L G IGI GLAFKPNTDD+RE+PA +I+ L+ GA VK YDP A EN R+ Sbjct: 303 LGDLEGKTIGILGLAFKPNTDDIRESPALKIIPKLLEAGANVKAYDPKAMENARKVL-PG 361 Query: 362 QLRWCGLPEEAAEGSDAVCLLTDWSVFKDIDLHKLAESMRRQVLIDGRNVFSKEQIEGTG 421 + +C + EE A+G DAV LLT+W+ F +I+ ++ M + ++IDGRN ++ G Sbjct: 362 DVVYCSVSEEVAKGVDAVALLTEWNEFIEIEWKDISIKMNQAIIIDGRNFLDVTYLQEQG 421 Query: 422 FEY 424 F Y Sbjct: 422 FRY 424 >O32271.1 TUAD_BACSU UDP-glucose 6-dehydrogenase tuaD EC=1.1.1.22 Length = 461 Score = 453 bits (1165), Expect = e-125, Method: Compositional matrix adjust. Identities = 226/435 (51%), Positives = 307/435 (70%), Gaps = 3/435 (0%) Query: 2 KLAVIGTGYVGLVSGVCFTLNGNHVICVDKDEEKIKKLSQMESPIYEPGIEALIEMNLRE 61 K+AVIGTGYVGLVSG CF GN V+C D DE KI+ L PIYEPG+ L+E N+ + Sbjct: 3 KIAVIGTGYVGLVSGTCFAEIGNKVVCCDIDESKIRSLKNGVIPIYEPGLADLVEKNVLD 62 Query: 62 GRLSFSSDLHESVRRSDIVILAVGTPSLPGGEADLRYIEGAATEIAQAMEGYKIIMTKST 121 RL+F++D+ ++R SDI+ +AVGTP GEADL Y++ AA I + + GYK+I+ KST Sbjct: 63 QRLTFTNDIPSAIRASDIIYIAVGTPMSKTGEADLTYVKAAAKTIGEHLNGYKVIVNKST 122 Query: 122 VPVGTNEKIRKII--ASHTNHPFDIVSAPEFLREGSAIQDTLHPDRIVIGLDNPELEPAM 179 VPVGT + ++ I+ AS + FD+VS PEFLREGSAI DT++ +R VIG + + + Sbjct: 123 VPVGTGKLVQSIVQKASKGRYSFDVVSNPEFLREGSAIHDTMNMERAVIGSTSHKAAAII 182 Query: 180 RQLHQGFTENVFVTDIRSAEMIKYASNAFLATKISFINEIANICEKVGADVTEVAEGMGM 239 +LHQ F V T++ SAEMIKYA+NAFLATKISFIN+IANICE+VGADV++VA+G+G+ Sbjct: 183 EELHQPFHAPVIKTNLESAEMIKYAANAFLATKISFINDIANICERVGADVSKVADGVGL 242 Query: 240 DRRIGSTFLQAGIGYGGSCFPKDTNALIQIAGNVDYEFKLLKSVVEVNKDQRFMIISKLH 299 D RIG FL+AGIG+GGSCFPKDT AL+QIA + Y FKL+++V+E N+ QR I+ KL Sbjct: 243 DSRIGRKFLKAGIGFGGSCFPKDTTALLQIAKSAGYPFKLIEAVIETNEKQRVHIVDKLL 302 Query: 300 ESLGNLRGAVIGIWGLAFKPNTDDVREAPAREIVESLVAEGATVKLYDPIAAENFRQQYD 359 +G+++G I + GLAFKPNT+DVR APA +I+ L GA VK YDPIA Sbjct: 303 TVMGSVKGRTISVLGLAFKPNTNDVRSAPALDIIPMLQQLGAHVKAYDPIAIPEASAILG 362 Query: 360 HPQLRWCGLPEEAAEGSDAVCLLTDWSVFKDIDLHKLAESMRRQVLIDGRNVFSKEQIEG 419 Q+ + A E +DA +LTDW K+++L K+ +++ V+IDGRN+FS E+++ Sbjct: 363 E-QVEYYTDVYAAMEDTDACLILTDWPEVKEMELVKVKTLLKQPVIIDGRNLFSLEEMQA 421 Query: 420 TGFEYHSVGRPQMGG 434 G+ YHS+GRP + G Sbjct: 422 AGYIYHSIGRPAVRG 436 >C3ED88.1 C3ED88_BACTU NDP-sugar dehydrogenase (Teichuronic acid biosynthesis) Length = 440 Score = 452 bits (1164), Expect = e-125, Method: Compositional matrix adjust. Identities = 222/434 (51%), Positives = 309/434 (71%), Gaps = 3/434 (0%) Query: 1 MKLAVIGTGYVGLVSGVCFTLNGNHVICVDKDEEKIKKLSQMESPIYEPGIEALIEMNLR 60 MK+A+ GTGYVGLV+GVC + G+ + C+D D++K++K+ SPIYEPG++ L++ N++ Sbjct: 1 MKIAIFGTGYVGLVTGVCLSDIGHKITCIDIDKKKVEKMKDGISPIYEPGLDKLMQKNIK 60 Query: 61 EGRLSFSSDLHESVRRSDIVILAVGTPSLPGGEADLRYIEGAATEIAQAMEGYKIIMTKS 120 GRLSF++ E ++ D++ +AVGTP G A+L+YIE A EIA ++E II+TKS Sbjct: 61 AGRLSFTTKYSEVLKNVDLIYIAVGTPQKDDGSANLQYIEQVALEIAGSIEKDMIIVTKS 120 Query: 121 TVPVGTNEKIRKIIASHTNH--PFDIVSAPEFLREGSAIQDTLHPDRIVIGLDNPELEPA 178 TVPVGTN+ ++ +I +H ++VS PEFLREGSAIQD+ H DRIVIG D+ E+ Sbjct: 121 TVPVGTNDYLKDLILTHLKFDVKINMVSNPEFLREGSAIQDSFHGDRIVIGADSEEIAKI 180 Query: 179 MRQLHQGFTENVFVTDIRSAEMIKYASNAFLATKISFINEIANICEKVGADVTEVAEGMG 238 ++++++ F +F T IRSAE+IKYASNAFLATKISFINEI+NICEKV A+V +VA GMG Sbjct: 181 VQEVNKPFRTPIFQTTIRSAELIKYASNAFLATKISFINEISNICEKVNANVEDVAYGMG 240 Query: 239 MDRRIGSTFLQAGIGYGGSCFPKDTNALIQIAGNVDYEFKLLKSVVEVNKDQRFMIISKL 298 +D+RIG+ FL+AGIGYGGSCFPKDT+ALIQIAGNVDYEF+LLKSV+ VN QR +++ K Sbjct: 241 LDKRIGTHFLKAGIGYGGSCFPKDTHALIQIAGNVDYEFELLKSVIRVNNKQRSLLVQKA 300 Query: 299 HESLGNLRGAVIGIWGLAFKPNTDDVREAPAREIVESLVAEGATVKLYDPIAAENFRQQY 358 + LGNL G + I GLAFKPNTDD+REA + I LVA GA VK YDP+A N ++ Sbjct: 301 KKKLGNLAGKQVAILGLAFKPNTDDIREAASIGIARELVAMGAVVKAYDPVAMNN-AAKF 359 Query: 359 DHPQLRWCGLPEEAAEGSDAVCLLTDWSVFKDIDLHKLAESMRRQVLIDGRNVFSKEQIE 418 ++ + +EA + +D +LT+W K+ L K + M + + DGRN + +++ Sbjct: 360 FPSEVEYTRSIDEAIQDADVTFILTEWDEIKNYPLSKYVKLMEQANIFDGRNCYDLNEVK 419 Query: 419 GTGFEYHSVGRPQM 432 Y S+GR ++ Sbjct: 420 KFELYYSSIGRLEV 433 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,663,487,400 Number of extensions: 112565612 Number of successful extensions: 337593 Number of sequences better than 1.0e-05: 2796 Number of HSP's gapped: 327414 Number of HSP's successfully gapped: 2810 Length of query: 446 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 307 Effective length of database: 1,751,175,118 Effective search space: 537610761226 Effective search space used: 537610761226 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)