BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0030.p (415 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B5JDP2.1 B5JDP2_9BACT Glycosyl hydrolase family 26 178 8e-43 B5JPX8.1 B5JPX8_9BACT Glycosyl hydrolase family 26 166 5e-39 B1ZXJ4.1 B1ZXJ4_OPITP Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 159 4e-37 A8Y8G5.1 A8Y8G5_STRAM Putative glycosidase 136 5e-30 B5YAS3.1 B5YAS3_DICT6 Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 133 3e-29 >B5JDP2.1 B5JDP2_9BACT Glycosyl hydrolase family 26 Length = 405 Score = 178 bits (452), Expect = 8e-43, Method: Compositional matrix adjust. Identities = 100/247 (40%), Positives = 134/247 (54%), Gaps = 15/247 (6%) Query: 103 RRNVVNSAISWYKNGGIVAMTFHEQLPGTSYQ------WANVQRTLSQAEFNKYVTPGTA 156 R + + A + G I+ + +H P +Y WA S E+N+ VT GT Sbjct: 150 RESTIEEAKRRHAMGHIITLMWHGCYPTDAYPCDGLSVWAEGNLP-SDEEWNELVTDGTE 208 Query: 157 QYKSLIADLDKVAVSLKSLRDAGVPVLWRPYHEMXXXXX---XXXXXXXXAELWNIMYDR 213 ++ +D +AV LK LRD +PVLWRPYHEM LW + YD Sbjct: 209 LNEAWKVGMDVIAVYLKELRDDNIPVLWRPYHEMNGEWFWWGHKKGEDGFKRLWLMTYDY 268 Query: 214 FVNVHQLNNLLWVWNPNAPNA----SSDPYELTYPGADKVDVLAADIYNNDYMNKYHDSL 269 N H+LNNL+WVWN NAP PYE +PGA+ VDVLA D+Y NDY +HD L Sbjct: 269 LTNHHELNNLIWVWNANAPRGLPGERGIPYEDYFPGAEYVDVLACDVYRNDYKQSHHDQL 328 Query: 270 LSLADGKPIAIGENGEMPSTAKLQLSQRQWVYMMNWGKMLYENNSTTTIKNFMNDSYTVT 329 ++LA+GKP+A+GE GEMPS L+ Q QW +MM WG +L+ +N IK + +T Sbjct: 329 VALAEGKPLALGEVGEMPSLEVLK-EQPQWTWMMTWGWILFFSNEAEFIKELYDSEQVLT 387 Query: 330 LDQYKAG 336 LD+ AG Sbjct: 388 LDEISAG 394 >B5JPX8.1 B5JPX8_9BACT Glycosyl hydrolase family 26 Length = 323 Score = 166 bits (419), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 103/302 (34%), Positives = 158/302 (52%), Gaps = 18/302 (5%) Query: 42 KLLDDLNAISGKGIITGQHDYLESPDEFSNRLKWTS---GKYAALHGYELGLISGQSEST 98 +LL+ L +ISG ++GQH P S RL+ +Y AL G + G S Sbjct: 12 ELLEFLYSISGDSTLSGQHCV---PLVGSTRLQGIEKSVDRYPALFGQDFGF-SEPGTWD 67 Query: 99 GAAQRRNVVNSAISWYKNGGIVAMTFH-----EQLPGTSYQWANVQRTLSQAEFNKYVTP 153 G R+N+V+ AI ++ G ++++ +H E+ P + ++Q L+ E+ +TP Sbjct: 68 GINYRQNIVDEAIRRHEEGFVISLMWHAVRPIEEEPVRFRE--SIQGELTDQEWKDLMTP 125 Query: 154 GTAQYKSLIADLDKVAVSLKSLRDAGVPVLWRPYHEMXXXXX---XXXXXXXXAELWNIM 210 GT + + +D +A LK LRDA VPVLWRPYHEM +LW ++ Sbjct: 126 GTEVNERWKSQVDVIAFFLKQLRDADVPVLWRPYHEMNGAWFWWGKKGGEDGYEKLWRML 185 Query: 211 YDRFVNVHQLNNLLWVWNPNAPNASSDPYELTYPGADKVDVLAADIYNNDYMNKYHDSLL 270 YDR + H+L+NL+WVWN N D Y YPG D VDVLA D+Y+ + ++ LL Sbjct: 186 YDRLTHFHKLDNLVWVWNANEVKQGVDSYATYYPGHDVVDVLATDVYSEAFNQANYEELL 245 Query: 271 SLADGKPIAIGENGEMPSTAKLQLSQRQWVYMMNWGKMLYENNSTTTIKNFMNDSYTVTL 330 LA KPIAIGE G++P+ KL+ Q +W + M W + ++ ++ + +T Sbjct: 246 ELAGDKPIAIGECGKVPTVEKLR-EQPRWAWFMIWERGYEGWDAFGAFRSLFHSEEVITH 304 Query: 331 DQ 332 D+ Sbjct: 305 DE 306 >B1ZXJ4.1 B1ZXJ4_OPITP Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 Length = 506 Score = 159 bits (403), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 104/306 (33%), Positives = 151/306 (49%), Gaps = 21/306 (6%) Query: 43 LLDDLNAISGKGIITGQHDYLESPDEFSNRLKWTSGKYAALHGYELG-LISGQSESTGAA 101 LL+ L +SG+ +TGQH+Y D S GK A+ + G ++G S+S A Sbjct: 39 LLELLYDLSGRHTLTGQHNYPNIKDRNSRFAAQHIGKMPAVFSTDFGHAVAGNSDSYLA- 97 Query: 102 QRRNVVNSAISWYKNGGIVAMTFH------------EQLPGTSYQ-WANVQRTLSQAEFN 148 R ++V I ++ G +V + +H LPG+ + +VQ L +F Sbjct: 98 -RPDIVQECIRQHQLGALVTICWHAVPPTADEPVTFRPLPGSDPKALKSVQGQLLDEQFR 156 Query: 149 KYVTPGTAQYKSLIADLDKVAVSLKSLRDAGVPVLWRPYHEMXXXXX---XXXXXXXXAE 205 +TPGTA + A +D +AV LK L A VPVLWRPYHEM Sbjct: 157 DVLTPGTALHTKWAAQVDAIAVFLKQLEAAHVPVLWRPYHEMNGGWFWWGGRTGEYSTER 216 Query: 206 LWNIMYDRFVNVHQLNNLLWVWNPNAPNASSDPYELTYPGADKVDVLAADIYNNDYMNKY 265 L+ ++DR VN HQL NL+WVW+ + + +E +PG D VDVL D+Y ND+ Y Sbjct: 217 LYRQIFDRLVNHHQLKNLVWVWSMDRVSKPGMEHEKFFPGIDYVDVLGLDVYGNDFAQSY 276 Query: 266 HDSLLSLADGKPIAIGENGEMPSTAKLQLSQRQWVYMMNWGKMLYENNSTTTIKNFMNDS 325 ++SL L+ GKP+A+ E G P+ L Q +W + + W M+ N S M D Sbjct: 277 YESLEKLSQGKPLALAEVGNPPALEILD-RQPKWTFYVTWAGMV-RNTSRKAYATLMADP 334 Query: 326 YTVTLD 331 + LD Sbjct: 335 RILNLD 340 >A8Y8G5.1 A8Y8G5_STRAM Putative glycosidase Length = 327 Score = 136 bits (342), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 95/287 (33%), Positives = 152/287 (52%), Gaps = 19/287 (6%) Query: 43 LLDDLNAISGKGIITGQHDY--LESPDEFSNRLKWTSGKYAALHGYELGLISGQSESTGA 100 +L+ L AISG I++GQH+ +P +++ ++K +G+Y L G +L + Sbjct: 33 VLNHLRAISGNHIVSGQHNKEPASAPGQYTQQVKDVTGQYPGLWGGDLMFAAAD-----V 87 Query: 101 AQRRNVVNSAISWYKNGGIVAMTFHEQLP---GTSYQWANVQRTLSQAEFNKYVTPGTAQ 157 A R+ VV+ A + + NG +V++T+H P T V+ TL+ A+F++ +T G+A Sbjct: 88 AGRQRVVDQARTEWANGSLVSLTWHVCPPTGGSTCAFEGGVKSTLTNAQFSQVLTEGSAL 147 Query: 158 YKSLIADLDKVAVSLKSLRDAGVPVLWRPYHEMXXXXX---XXXXXXXXAELWNIMYDRF 214 + LD+V L+ L +AGVPVL+RP HEM A L+ I D Sbjct: 148 NSAWKRRLDEVVPYLQQLENAGVPVLFRPLHEMNESWNWWGNRPGANGSARLYQITRDHL 207 Query: 215 VNVHQLNNLLWVWN-PNAPNASSDPYELTYPGADKVDVLAADI-YNNDYMNKYHDSLLSL 272 L+NL+WVWN + P + + Y YPG VDV++ D+ Y + + + + S+ Sbjct: 208 AGTKGLDNLIWVWNVQDNPAGNWNSY---YPGDQYVDVVSLDVWYKSHPSSADYQQMRSI 264 Query: 273 ADGKPIAIGENGEMPSTAKLQLSQRQWVYMMNWGKMLYENNSTTTIK 319 A KP+A+ E G+MP+ A L SQ +W + M W + L NNS I+ Sbjct: 265 AGTKPMALAELGKMPTAALLD-SQTRWTWFMMWSEHLRGNNSNAEIQ 310 >B5YAS3.1 B5YAS3_DICT6 Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 Length = 469 Score = 133 bits (335), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 100/314 (31%), Positives = 152/314 (48%), Gaps = 41/314 (13%) Query: 42 KLLDDLNAISGKGIITGQHDYLESPDEFSNRLKWT-SGKYAALHGYELGLISGQSESTGA 100 KL+D L +I GK ++GQ Y D F W + K+ A+ G+++ S GA Sbjct: 166 KLMDYLVSIYGKYTLSGQMGY---KDAF---WIWNITDKFPAICGFDMMDYSPSRVERGA 219 Query: 101 AQRRNVVNSAISWYKNGGIVAMTFHEQLPGTSY-----QW-----ANVQRTLSQAEFNKY 150 + R V AI W+ GGIV +H P Y +W N + FN Sbjct: 220 SSRD--VEDAIDWWNMGGIVQFQWHWNAPKGLYDTPGKEWWRGFYTNATSFDIEYAFNH- 276 Query: 151 VTPGTAQYKSLIADLDKVAVSLKSLRDAGVPVLWRPYHEMXXXXXXXXXXXXXA--ELWN 208 P + YK +I D+D +AV LK L++A VP+LWRP HE A +LW Sbjct: 277 --PESEDYKLIIRDIDAIAVQLKRLQEAKVPILWRPLHEAEGRWFWWGAKGPEACKKLWR 334 Query: 209 IMYDRFVNVHQLNNLLWVW-NPNAPNASSDPYELTYPGADKVDVLAADIY--NNDY---M 262 +++DR VN H++NNL+WVW ++P+A YPG + VD++ ADIY + DY Sbjct: 335 LLFDRLVNYHKINNLIWVWTTTDSPDALK-----WYPGDEYVDIVGADIYLKDKDYSPST 389 Query: 263 NKYHDSLLSLADGKPIAIGENGEMPSTAKLQLSQRQWVYMMNWGKMLYEN----NSTTTI 318 +++ + K +A+ ENG +P ++ + WV+ M W +EN N + I Sbjct: 390 GMFYNIVKLFGGKKLVALTENGIIPDPDLMKEQKAYWVWFMTWSG--FENDPNKNEISHI 447 Query: 319 KNFMNDSYTVTLDQ 332 K N + +T D+ Sbjct: 448 KKVFNHPFVITKDE 461 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,080,871,163 Number of extensions: 77048876 Number of successful extensions: 195045 Number of sequences better than 1.0e-05: 130 Number of HSP's gapped: 194765 Number of HSP's successfully gapped: 130 Length of query: 415 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 277 Effective length of database: 1,760,596,133 Effective search space: 487685128841 Effective search space used: 487685128841 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)